FastQCFastQC Report
Sat 18 Jun 2016
SRR3553433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497488
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127132.5554385231402565No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32630.6558952175730871No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24620.494886308815489No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT21520.4325732479979417Illumina PCR Primer Index 8 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13210.26553404303219375No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGT9380.188547261441482No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC8530.17146142218505772No Hit
AATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT6990.14050590164988905RNA PCR Primer, Index 40 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5590.11236451934519025No Hit
AAGCAAGGGACCTGTGAACTCAAGACCAGTGTGGTAAGTTACAGGACACAA5420.10894735149390537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTG600.045.0000041
TATCAAC302.1630094E-645.00000444
CAGCGCG302.1630094E-645.0000041
AATTTCG302.1630094E-645.00000416
GTCGAAG302.1630094E-645.00000436
TAGCGAG1150.045.0000041
TATATCA302.1630094E-645.00000442
ACGGGTT650.045.0000045
AGAACCG302.1630094E-645.0000041
ATCGTTA302.1630094E-645.00000410
ATCGTAG302.1630094E-645.0000041
GCATTAC302.1630094E-645.00000435
AATTGCG302.1630094E-645.0000041
CGAGGTA302.1630094E-645.00000429
CGTAGAC302.1630094E-645.0000048
ACTACCG302.1630094E-645.0000041
GACGGCG302.1630094E-645.0000049
TATAGCG600.045.0000041
GACGCAT302.1630094E-645.00000432
ACTCACG302.1630094E-645.0000041
Produced by FastQC (version 0.11.5)