##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553433_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497488
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.496751680442543	31.0	31.0	34.0	30.0	34.0
2	31.71144228604509	31.0	31.0	34.0	30.0	34.0
3	31.896264834528672	33.0	31.0	34.0	30.0	34.0
4	35.648361367510375	37.0	35.0	37.0	33.0	37.0
5	35.40669925706751	37.0	35.0	37.0	33.0	37.0
6	32.48081159746567	37.0	35.0	37.0	25.0	37.0
7	34.116177676647474	37.0	35.0	37.0	26.0	37.0
8	35.27881275528254	37.0	35.0	37.0	32.0	37.0
9	37.12547639340045	39.0	37.0	39.0	34.0	39.0
10	36.57117960634226	38.0	35.0	39.0	32.0	39.0
11	36.65593340944907	39.0	35.0	39.0	32.0	39.0
12	36.72508884636413	39.0	35.0	39.0	32.0	39.0
13	36.733434776316216	39.0	37.0	39.0	32.0	39.0
14	37.85897549287621	39.0	37.0	41.0	33.0	41.0
15	37.919686505001124	40.0	37.0	41.0	33.0	41.0
16	37.82317362428842	40.0	37.0	41.0	33.0	41.0
17	37.79748456244171	39.0	37.0	41.0	33.0	41.0
18	37.645354259801245	39.0	37.0	41.0	33.0	41.0
19	37.597186263789276	39.0	36.0	41.0	32.0	41.0
20	37.48215836361882	39.0	36.0	41.0	32.0	41.0
21	37.43144357250828	39.0	36.0	40.0	32.0	41.0
22	37.37582213038304	39.0	36.0	40.0	32.0	41.0
23	37.23622881355932	39.0	35.0	40.0	32.0	41.0
24	37.10537339594121	39.0	35.0	40.0	31.0	41.0
25	37.11453743607886	39.0	35.0	40.0	32.0	41.0
26	37.0334942752388	39.0	35.0	40.0	31.0	41.0
27	36.91856285980767	39.0	35.0	40.0	31.0	41.0
28	36.87863626861351	39.0	35.0	40.0	31.0	41.0
29	36.62549448428907	39.0	35.0	40.0	31.0	41.0
30	36.65443789598945	39.0	35.0	40.0	31.0	41.0
31	36.2706417039205	38.0	35.0	40.0	30.0	41.0
32	36.332008008233366	38.0	35.0	40.0	30.0	41.0
33	36.320853970347024	38.0	35.0	40.0	30.0	41.0
34	36.288523542276394	38.0	35.0	40.0	30.0	41.0
35	36.23632127810118	38.0	35.0	40.0	30.0	41.0
36	36.10728097964172	38.0	35.0	40.0	30.0	41.0
37	36.051754012157076	38.0	35.0	40.0	30.0	41.0
38	36.00207241179687	38.0	35.0	40.0	30.0	41.0
39	35.91410446081112	38.0	35.0	40.0	29.0	41.0
40	35.79911877271412	38.0	35.0	40.0	28.0	41.0
41	35.81503071430868	38.0	35.0	40.0	28.0	41.0
42	35.7161378766925	38.0	35.0	40.0	28.0	41.0
43	35.60877850320008	38.0	34.0	40.0	27.0	41.0
44	35.428408323416846	38.0	34.0	40.0	27.0	41.0
45	35.225683835590004	38.0	34.0	40.0	26.0	41.0
46	34.91474769240665	38.0	33.0	40.0	25.0	41.0
47	34.93356422667481	38.0	33.0	40.0	25.0	41.0
48	34.950609863956515	38.0	34.0	40.0	26.0	41.0
49	34.853487923326796	37.0	33.0	40.0	26.0	41.0
50	34.70253352844692	37.0	33.0	40.0	25.0	41.0
51	33.63620027015727	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	4.0
12	6.0
13	14.0
14	12.0
15	36.0
16	65.0
17	134.0
18	276.0
19	564.0
20	964.0
21	1448.0
22	2004.0
23	2590.0
24	3156.0
25	3731.0
26	4392.0
27	5266.0
28	6521.0
29	8499.0
30	10917.0
31	14263.0
32	18425.0
33	24206.0
34	33841.0
35	48897.0
36	53486.0
37	69446.0
38	91663.0
39	92638.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.53333547743865	35.406080146656805	20.99226514006368	24.068319235840864
2	21.81901875020101	33.75277393625575	20.35023960376934	24.077967709773905
3	20.42883446434889	34.24384105747274	19.94239057022481	25.38493390795356
4	19.83203614961567	36.382988132377065	18.303758080596918	25.481217637410346
5	17.047245360692116	39.03893159231981	16.427531920367926	27.486291126620138
6	16.670552857556366	45.995280288167756	16.45225613482134	20.881910719454538
7	83.11396455793908	10.098334030167562	3.6396857813655807	3.1480156305277713
8	84.20404110249895	9.260725886855562	2.9363522336217156	3.598880777023768
9	78.02982182484803	13.488566558389348	5.555108866947545	2.926502749815071
10	27.868410896343228	49.441594571125336	11.049914771813592	11.640079760717846
11	18.359236805711895	31.07492040009005	34.343742964654425	16.222099829543627
12	22.77341363007751	27.653732351333097	32.61465603190429	16.958197986685107
13	23.134829382819284	29.840518766281797	29.466640401376516	17.5580114495224
14	19.571125333676388	32.73847811404496	28.651545363908276	19.038851188370373
15	16.52964493615926	35.30658025922233	28.711647316116167	19.452127488502235
16	20.25335284469173	34.34394397452803	26.85893931109896	18.54376386968128
17	18.100135078635063	34.480027658958605	28.04489756536841	19.374939697037917
18	18.60708197986685	33.98011610330299	27.70117068150387	19.71163123532628
19	18.907189721159103	35.357636767118	27.513427459556812	18.221746052166083
20	19.423584086450326	34.4756054417393	28.063993503360884	18.03681696844949
21	20.26501141736082	34.83038786865211	26.39420448332422	18.510396230662852
22	19.446499212041296	32.72983468947995	26.777731322162545	21.045934776316212
23	18.353809539124562	34.25288650178497	25.901529283118386	21.491774675972085
24	15.682589328787829	35.494524491043	29.413975814492012	19.408910365677162
25	18.34697520342199	36.569324285208886	25.54815392532081	19.53554658604831
26	20.219985205673304	34.95018975332068	25.209653298170004	19.620171742836007
27	17.242023928215353	35.709806065673945	26.4539044157849	20.594265590325797
28	19.740978676872608	35.062956292413084	26.83924034348567	18.356824687228638
29	18.648087994082267	35.52929919917666	26.266563213585055	19.556049593156015
30	20.31204772778439	35.21471874698485	25.57709934711993	18.89613417811083
31	21.016788344643487	35.64809603447721	23.824494259158012	19.510621361721288
32	20.212346830476314	35.279644936159265	24.168221143022546	20.339787090341876
33	19.862388640530025	34.70355063840736	25.147340237352463	20.28672048371016
34	19.512229440710126	34.13006946901232	26.565665905509277	19.792035184768274
35	20.478684913002926	35.29833885440453	24.18771910076223	20.035257131830317
36	20.54240504293571	37.013958125623134	25.075981732222687	17.367655099218474
37	19.290113530376612	36.54460007075547	24.905324349532048	19.25996204933586
38	20.123500466342907	34.89692213681536	26.46254784034992	18.517029556491813
39	20.601501945775578	33.66372656224873	25.386139967195188	20.348631524780497
40	20.771154279098187	34.50254076480237	24.147718135914836	20.578586820184608
41	19.65715755957933	31.55834914611006	27.088693596629465	21.69579969768115
42	20.71044929726948	33.013258611262984	24.693660952625994	21.582631138841542
43	19.190010613321327	34.2259511787219	25.799014247579844	20.785023960376932
44	20.31928408323417	32.343292702537546	25.40342681632522	21.933996397903066
45	21.560721062618597	33.9642363232882	25.187341202199853	19.28770141189335
46	19.496550670568936	33.21145434663751	27.277039848197344	20.01495513459621
47	18.89693821760525	34.386960087479494	26.298523783488243	20.41757791142701
48	19.750024121184833	31.777248898465892	28.232037757694656	20.240689222654616
49	20.091137876692503	32.675361013732996	26.012687743221946	21.220813366352555
50	18.817941337278487	31.115524394558257	27.19181166178883	22.874722606374426
51	19.08870163702441	30.41761811340173	28.21655999742707	22.277120252146783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14555.0
1	11140.5
2	7726.0
3	4834.0
4	1942.0
5	1599.5
6	1257.0
7	1362.5
8	1468.0
9	1673.0
10	1878.0
11	2208.5
12	2539.0
13	2846.5
14	3154.0
15	3060.0
16	2966.0
17	3218.0
18	3470.0
19	3456.0
20	3442.0
21	3464.5
22	3487.0
23	3531.5
24	3576.0
25	3916.5
26	6037.5
27	7818.0
28	8437.0
29	9056.0
30	11233.0
31	13410.0
32	13789.0
33	14168.0
34	16651.0
35	19134.0
36	20294.5
37	21455.0
38	23450.0
39	25445.0
40	25100.0
41	24755.0
42	27843.0
43	30931.0
44	32635.0
45	34339.0
46	37458.5
47	40578.0
48	42103.0
49	43628.0
50	42258.0
51	40888.0
52	35851.0
53	30814.0
54	27220.5
55	23627.0
56	20145.5
57	16664.0
58	14777.5
59	12891.0
60	11997.0
61	11103.0
62	9072.0
63	7041.0
64	6098.5
65	5156.0
66	4127.0
67	3098.0
68	2530.5
69	1963.0
70	1585.5
71	1208.0
72	1139.5
73	1071.0
74	922.5
75	530.5
76	287.0
77	203.5
78	120.0
79	167.5
80	215.0
81	132.0
82	49.0
83	43.0
84	37.0
85	32.0
86	27.0
87	19.5
88	12.0
89	7.0
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	497488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.296594600969588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16901905383993	21.55323853320036
2	9.627778058435995	5.4486666525534675
3	3.727095399674608	3.163923226911933
4	1.9811518858170487	2.242394070236456
5	1.2414758185567647	1.756476897230382
6	0.8927754776435639	1.515750345634015
7	0.6577997357944236	1.3029444716679794
8	0.5028533762867676	1.138323050601238
9	0.4380435227562036	1.11556259829116
>10	3.6227538397240746	22.49735591993229
>50	0.7170682259950797	14.203208708115211
>100	0.4149933464384764	18.872079317708
>500	0.0035961295185309916	0.730827529708718
>1k	0.002876903614824793	1.8719441989030319
>5k	0.0	0.0
>10k+	7.192259037061983E-4	2.5873044793057454
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12713	2.5554385231402565	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3263	0.6558952175730871	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2462	0.494886308815489	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	2152	0.4325732479979417	Illumina PCR Primer Index 8 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1321	0.26553404303219375	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGT	938	0.188547261441482	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	853	0.17146142218505772	No Hit
AATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT	699	0.14050590164988905	RNA PCR Primer, Index 40 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	559	0.11236451934519025	No Hit
AAGCAAGGGACCTGTGAACTCAAGACCAGTGTGGTAAGTTACAGGACACAA	542	0.10894735149390537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0100987360499147E-4	0.0	0.0	0.541319589618242	0.0
2	2.0100987360499147E-4	0.0	0.0	0.6948911330524555	0.0
3	2.0100987360499147E-4	0.0	0.0	0.8846444537355674	0.0
4	2.0100987360499147E-4	0.0	0.0	1.8281848004373975	0.0
5	2.0100987360499147E-4	0.0	0.0	1.9654745441096066	0.0
6	2.0100987360499147E-4	0.0	0.0	2.780167561830637	0.0
7	2.0100987360499147E-4	0.0	0.0	3.4298314733219697	0.0
8	2.0100987360499147E-4	0.0	0.0	4.060801466568038	0.0
9	2.0100987360499147E-4	0.0	0.0	5.097610394622584	0.0
10	2.0100987360499147E-4	0.0	0.0	6.475935097932011	0.0
11	2.0100987360499147E-4	0.0	0.0	7.911145595471649	0.0
12	2.0100987360499147E-4	0.0	0.0	8.583322612806741	0.0
13	2.0100987360499147E-4	0.0	0.0	8.868756633325829	0.0
14	2.0100987360499147E-4	0.0	0.0	9.170271443733316	0.0
15	2.0100987360499147E-4	0.0	0.0	9.416910558646642	0.0
16	2.0100987360499147E-4	0.0	0.0	9.909183739105265	0.0
17	2.0100987360499147E-4	0.0	0.0	10.605883961020165	0.0
18	2.0100987360499147E-4	0.0	0.0	11.58299295661403	0.0
19	2.0100987360499147E-4	0.0	0.0	11.957474351140128	0.0
20	2.0100987360499147E-4	0.0	0.0	12.37316276975525	0.0
21	2.0100987360499147E-4	0.0	0.0	12.776388576206863	0.0
22	2.0100987360499147E-4	0.0	0.0	13.254189045765928	0.0
23	2.0100987360499147E-4	0.0	0.0	13.741034959637217	0.0
24	2.0100987360499147E-4	0.0	0.0	14.063052777152414	0.0
25	2.0100987360499147E-4	0.0	0.0	14.348285787797897	0.0
26	2.0100987360499147E-4	0.0	0.0	14.609196603737175	0.0
27	2.0100987360499147E-4	0.0	0.0	14.959556813430675	0.0
28	2.0100987360499147E-4	0.0	0.0	15.276147364358538	0.0
29	2.0100987360499147E-4	0.0	0.0	15.598165181873734	0.0
30	2.0100987360499147E-4	0.0	0.0	16.012647541247226	0.0
31	2.0100987360499147E-4	0.0	0.0	16.324212845334962	0.0
32	2.0100987360499147E-4	0.0	0.0	16.641004406136428	0.0
33	2.0100987360499147E-4	0.0	0.0	16.9298555945068	0.0
34	2.0100987360499147E-4	0.0	0.0	17.262325925449456	0.0
35	2.0100987360499147E-4	0.0	0.0	17.59901746373782	0.0
36	2.0100987360499147E-4	0.0	0.0	17.921839320747434	0.0
37	2.0100987360499147E-4	0.0	0.0	18.2466712764931	0.0
38	2.0100987360499147E-4	0.0	0.0	18.560045669443284	0.0
39	2.0100987360499147E-4	0.0	0.0	18.898948316341297	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	60	0.0	45.000004	1
TATCAAC	30	2.1630094E-6	45.000004	44
CAGCGCG	30	2.1630094E-6	45.000004	1
AATTTCG	30	2.1630094E-6	45.000004	16
GTCGAAG	30	2.1630094E-6	45.000004	36
TAGCGAG	115	0.0	45.000004	1
TATATCA	30	2.1630094E-6	45.000004	42
ACGGGTT	65	0.0	45.000004	5
AGAACCG	30	2.1630094E-6	45.000004	1
ATCGTTA	30	2.1630094E-6	45.000004	10
ATCGTAG	30	2.1630094E-6	45.000004	1
GCATTAC	30	2.1630094E-6	45.000004	35
AATTGCG	30	2.1630094E-6	45.000004	1
CGAGGTA	30	2.1630094E-6	45.000004	29
CGTAGAC	30	2.1630094E-6	45.000004	8
ACTACCG	30	2.1630094E-6	45.000004	1
GACGGCG	30	2.1630094E-6	45.000004	9
TATAGCG	60	0.0	45.000004	1
GACGCAT	30	2.1630094E-6	45.000004	32
ACTCACG	30	2.1630094E-6	45.000004	1
>>END_MODULE