FastQCFastQC Report
Sat 18 Jun 2016
SRR3553432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501621
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109692.1867106839625934No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27300.5442355882229811No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT24230.48303400375981065No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22840.4553238401103622No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11720.2336425309147743No Hit
CTATTTGGGTTTGTGTTTTGTTTTTAATTGAAAGATTGGAATCCCAAGCAT8820.17582995927204004No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGT6400.1275863650046549No Hit
GCATCTGGGGTCCCTTCCCCCACCCATCACAGAAACACTTTCCTTTACCCT5880.11721997284802671No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT5630.11223613046503238No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA5050.10067361613648551No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC207.029479E-445.00000436
ACGCGAG302.1630458E-645.0000041
TCCGGCA207.029479E-445.00000433
TCACGAT302.1630458E-645.00000432
CTATGCA207.029479E-445.00000429
ACGCCTA207.029479E-445.00000432
CTCGTGA207.029479E-445.00000430
ATTAGCG406.8012014E-945.0000041
CGTATAG207.029479E-445.0000041
CTATCCA207.029479E-445.00000413
CGGCCGT600.045.00000441
TCACACG207.029479E-445.0000041
CGTGATC207.029479E-445.00000440
CGTTGCA207.029479E-445.00000425
GGCCGTA600.045.00000442
ATTACGT207.029479E-445.00000442
GGCCGCT207.029479E-445.00000434
GATCCGT207.029479E-445.0000049
CCAATAT207.029479E-445.00000412
CCTAATC207.029479E-445.00000435