SRR3553428_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368448
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18104	4.913583463609519	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4167	1.130960135487233	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGT	3666	0.9949843668577384	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3619	0.9822281570262289	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCC	3343	0.9073193503560882	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	2874	0.780028660760813	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCT	2741	0.7439313010248394	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1763	0.47849357304151463	No Hit
GCAGGGATTGCTTTAGTGAAACAGCTTGTGTCTTTTATTTCGTTTATTGTG	835	0.22662628104915755	No Hit
CTCCACGGGGTAAGAAACCAATCAAGATCAAGGAAGGTGAAATAATCTATA	824	0.2236407851311447	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTT	823	0.22336937641132532	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCT	707	0.1918859649122807	No Hit
AAACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCT	603	0.16365945805106827	No Hit
AATTTTGGGATGTATGGGAAACCATGAACTGTACATGCGAAGGAGAAAGCC	542	0.1471035261420879	No Hit
AACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	523	0.14194676046552024	No Hit
AGCGCGGGAAAGCTATTTTTATTTTATGCCGACTTTTGATTATTTTGATGC	482	0.13081900295292687	No Hit
AGGAAAGGGATTTTTATTTACTTTTTTGTAAAGTGATTTCCAGTCTTGTGT	481	0.13054759423310752	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	475	0.1289191419141914	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTAT	474	0.12864773319437206	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	471	0.12783350703491403	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	443	0.12023406287997221	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTT	436	0.11833420184123675	No Hit
AAGCTGGGAAGAATATAGTTTGACTTGGGTCTGGGATGCTCTTCTTTTTTT	432	0.11724856696195936	No Hit
GCAGCAGGGATTTTTTATTTGCTTATTCCTTCATGTCGGACGAGGCTTATA	432	0.11724856696195936	No Hit
GGAGGAGGGGAGGAACTGGGAGGAGCTGGGGTAAGGAAAACTATAATTAGA	423	0.1148058884835852	No Hit
AAAGTTGGGGATGTATCTGGGGAACTGAGGAGAGCAGAACGGAAAGGTTCT	403	0.10937771408719821	No Hit
AAACTCGGGATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACA	372	0.10096404377279833	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	70	0.0	45.000004	29
TGATCCG	35	1.2089185E-7	45.000004	25
GTGCATT	35	1.2089185E-7	45.000004	16
TCGCAGC	35	1.2089185E-7	45.000004	45
CGTATTT	35	1.2089185E-7	45.000004	17
AGCTTAG	35	1.2089185E-7	45.000004	36
TTGTACG	35	1.2089185E-7	45.000004	1
GACCACA	35	1.2089185E-7	45.000004	41
ATTCCCG	35	1.2089185E-7	45.000004	1
GCATTAT	35	1.2089185E-7	45.000004	18
TCGTATT	35	1.2089185E-7	45.000004	16
CTACAAC	35	1.2089185E-7	45.000004	35
GTAAGTC	35	1.2089185E-7	45.000004	39
TTGCACG	35	1.2089185E-7	45.000004	1
TAATGCG	35	1.2089185E-7	45.000004	1
AATACAT	35	1.2089185E-7	45.000004	31
TGACTAT	35	1.2089185E-7	45.000004	22
CTAGCAG	35	1.2089185E-7	45.000004	1
CGGGTTA	20	7.02704E-4	45.0	6
AGGTATT	20	7.02704E-4	45.0	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content