##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368448
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.372858585200625	31.0	31.0	34.0	30.0	34.0
2	31.572707681952405	31.0	31.0	34.0	30.0	34.0
3	31.755504168837938	31.0	31.0	34.0	30.0	34.0
4	35.50388928695501	37.0	35.0	37.0	33.0	37.0
5	35.21289842800069	37.0	35.0	37.0	33.0	37.0
6	33.87917426611082	37.0	35.0	37.0	30.0	37.0
7	34.6639118681605	37.0	35.0	37.0	32.0	37.0
8	35.37110799895779	37.0	35.0	37.0	32.0	37.0
9	36.983316505992704	39.0	37.0	39.0	33.0	39.0
10	36.450372915581035	38.0	35.0	39.0	32.0	39.0
11	36.523978960396036	38.0	35.0	39.0	32.0	39.0
12	36.50331661455619	38.0	35.0	39.0	32.0	39.0
13	36.48914907938162	38.0	35.0	39.0	32.0	39.0
14	37.55938422789647	39.0	36.0	40.0	32.0	41.0
15	37.608680193677266	39.0	36.0	41.0	32.0	41.0
16	37.4414082855654	39.0	36.0	40.0	32.0	41.0
17	37.38234974813271	39.0	36.0	40.0	32.0	41.0
18	37.12699214000347	39.0	36.0	40.0	32.0	41.0
19	36.90873067569915	39.0	35.0	40.0	31.0	41.0
20	36.83468494875803	39.0	35.0	40.0	31.0	41.0
21	36.83632697151294	39.0	35.0	40.0	31.0	41.0
22	36.790097924266114	38.0	35.0	40.0	31.0	41.0
23	36.55010204967865	38.0	35.0	40.0	31.0	41.0
24	36.39406646256731	38.0	35.0	40.0	30.0	41.0
25	36.47905538909154	38.0	35.0	40.0	31.0	41.0
26	36.37273645127671	38.0	35.0	40.0	30.0	41.0
27	36.32277553413236	38.0	35.0	40.0	30.0	41.0
28	36.18925872850443	38.0	35.0	40.0	30.0	41.0
29	35.959828795379536	38.0	35.0	40.0	30.0	41.0
30	36.039050286607605	38.0	35.0	40.0	30.0	41.0
31	35.80414061142957	38.0	35.0	40.0	29.0	41.0
32	35.73910022581205	38.0	35.0	40.0	29.0	41.0
33	35.62994235278791	38.0	35.0	40.0	29.0	41.0
34	35.49440626628452	38.0	34.0	40.0	27.0	41.0
35	35.459288692027094	38.0	34.0	40.0	27.0	41.0
36	35.32118779312142	38.0	34.0	40.0	27.0	41.0
37	35.25387028834462	38.0	34.0	40.0	27.0	41.0
38	35.210078491401774	38.0	34.0	40.0	26.0	41.0
39	35.12910641393087	38.0	34.0	40.0	26.0	41.0
40	34.9755080771235	38.0	34.0	40.0	25.0	41.0
41	34.83072780962307	37.0	33.0	40.0	25.0	41.0
42	34.79047518238666	37.0	33.0	40.0	25.0	41.0
43	34.753341041340974	37.0	33.0	40.0	24.0	41.0
44	34.64223716779573	37.0	33.0	40.0	24.0	41.0
45	34.43477505645301	37.0	33.0	40.0	23.0	41.0
46	34.22382805714782	37.0	33.0	40.0	23.0	41.0
47	34.159061794337326	37.0	33.0	40.0	23.0	41.0
48	34.05081585461178	37.0	33.0	40.0	23.0	41.0
49	33.889810773840544	36.0	33.0	40.0	22.0	41.0
50	33.76766870766024	36.0	33.0	40.0	22.0	41.0
51	32.57040885009554	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	15.0
15	30.0
16	53.0
17	133.0
18	283.0
19	590.0
20	983.0
21	1614.0
22	2127.0
23	2721.0
24	3271.0
25	3567.0
26	4020.0
27	4831.0
28	5929.0
29	7280.0
30	9376.0
31	12006.0
32	15383.0
33	19971.0
34	27964.0
35	42914.0
36	39872.0
37	48395.0
38	61959.0
39	53151.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.13322911238492	39.97226202883446	18.420781222859127	22.473727635921488
2	19.282232499565747	40.8817526489491	18.888418447107867	20.94759640437728
3	17.46677957269411	37.98310752127844	22.55189334722946	21.998219558797985
4	20.637104828903944	37.28314443286434	18.49107608129234	23.58867465693938
5	14.794489317352788	44.12264417231197	16.22752735799896	24.855339152336285
6	16.825169359041166	44.71187250303978	20.13608433211742	18.326873805801632
7	71.93063878756296	18.34424396387007	5.415418186555498	4.309699062011465
8	73.95534783741532	14.46309927045336	4.291786086503388	7.289766805627932
9	68.19144085461177	18.756513809275667	8.240511551155114	4.811533784957443
10	26.958213913496614	48.042057495223204	11.294673875282266	13.705054715997916
11	16.65689595275317	35.1856978461004	30.728352440507205	17.42905376063922
12	21.13568264721209	32.09028139656071	32.48273840541949	14.291297550807712
13	22.511996265416016	34.721588935209304	25.37671530310926	17.389699496265415
14	15.07756861212437	36.78158111863818	30.382577731457356	17.758272537780094
15	14.682126107347576	39.299982629841935	26.95034306062185	19.06754820218864
16	16.272852614208787	38.112569480632274	26.339402032308495	19.275175872850443
17	14.74373588674657	37.191408285565394	27.907330206704884	20.15752562098315
18	16.424570088587807	35.76054151467778	28.782623328122288	19.032265068612126
19	17.255352179954837	40.4903812749696	27.339000347403164	14.9152661976724
20	16.81567005384749	41.54778964738579	25.403041948931737	16.23349834983498
21	16.76844493659892	39.25302892131318	25.95725855480285	18.021267587285045
22	16.304064616988015	37.72201233281223	25.678250390828556	20.2956726593712
23	15.416015285739102	39.606674483237796	23.972175178044118	21.005135052978982
24	12.746167708876152	42.4469667361473	25.25865250998784	19.54821304498871
25	15.60057321521626	39.32685209310405	24.085352614208787	20.987222077470904
26	17.32944676046552	39.541536390481156	23.30830944936599	19.820707399687336
27	14.720394736842104	38.767478721556365	25.80608389786347	20.70604264373806
28	16.975801198540907	38.736538127496964	25.35500260552371	18.932658068438425
29	17.063194806322738	41.38358737189509	22.963891783915233	18.589326037866943
30	19.77293946499913	37.36185296161195	21.97976376585027	20.885443807538646
31	18.214510595796423	42.54250260552371	20.045976637137397	19.19701016154247
32	19.55146994962654	36.258576515546295	21.91272581205489	22.277227722772277
33	17.524861038735455	38.535152857391004	21.44753126628452	22.492454837589023
34	16.002800937988535	39.30079685600139	22.608617769671703	22.08778443633837
35	18.9169163626889	36.87738839673441	23.93797767934688	20.267717561229805
36	18.564085026923745	40.255341323606046	23.939877540385616	17.240696109084595
37	19.878788865728676	39.78417578599965	21.793034566614555	18.544000781657115
38	18.76601311446934	37.70409935730415	24.985072520409936	18.54481500781657
39	18.830336981066527	35.575983585200625	24.80024318221296	20.79343625151989
40	17.9224748132708	37.615348705923225	24.801328817092237	19.66084766371374
41	15.885009553586936	36.65999001215911	28.203979937467434	19.25102049678652
42	18.56652770540212	35.23102310231023	25.822911238492267	20.379537953795378
43	17.70154811533785	36.286803022407504	26.132045770366506	19.879603091888136
44	18.564899253083205	33.86122329338197	24.739176220253604	22.834701233281223
45	20.52175612298072	37.07388830988362	24.133391957616816	18.270963609518848
46	17.160087719298247	35.353157026228935	29.173180475942328	18.313574778530484
47	17.764786347055757	36.337827861733544	24.64391175959701	21.25347403161369
48	17.84783741532048	34.728916970644434	29.19027922529095	18.23296638874414
49	18.652835678304672	34.09002084418968	26.150501563314226	21.106641914191417
50	18.615381274969604	31.134922702796597	26.428152683689422	23.82154333854438
51	17.05505254472816	32.039256557234665	28.737569480632274	22.168121417404897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20320.0
1	15514.5
2	10709.0
3	6592.0
4	2475.0
5	2019.5
6	1564.0
7	1640.5
8	1717.0
9	1939.0
10	2161.0
11	2663.5
12	3166.0
13	3152.5
14	3139.0
15	3310.0
16	3481.0
17	3552.0
18	3623.0
19	3402.5
20	3182.0
21	3227.0
22	3272.0
23	3375.5
24	3479.0
25	3971.0
26	4717.0
27	4971.0
28	6588.0
29	8205.0
30	8080.0
31	7955.0
32	8791.5
33	9628.0
34	11220.5
35	12813.0
36	12364.5
37	11916.0
38	13126.0
39	14336.0
40	14257.0
41	14178.0
42	15926.0
43	17674.0
44	25086.5
45	32499.0
46	31650.0
47	30801.0
48	32284.0
49	33767.0
50	31757.0
51	29747.0
52	25501.0
53	21255.0
54	17632.5
55	14010.0
56	12157.0
57	10304.0
58	9157.5
59	8011.0
60	6856.0
61	5701.0
62	5101.0
63	4501.0
64	4033.0
65	3565.0
66	3000.0
67	2435.0
68	1876.0
69	1317.0
70	1095.5
71	874.0
72	673.0
73	472.0
74	391.0
75	276.5
76	243.0
77	187.5
78	132.0
79	77.5
80	23.0
81	22.5
82	22.0
83	15.5
84	9.0
85	11.5
86	14.0
87	9.0
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.021548578262166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02154823883883	21.02219958391061
2	10.40976063763551	5.833952267911697
3	3.9685320893400786	3.3361324417750584
4	2.201049671790077	2.4670728120493464
5	1.355945249193267	1.899784283486647
6	0.9555321873456972	1.6065294963480339
7	0.7428570526347613	1.4571203490976732
8	0.5940857760477737	1.331776274654182
9	0.44831009111565595	1.1306108696690245
>10	3.423731170102956	19.649854548697828
>50	0.5221963843234718	10.190046595493675
>100	0.34147449988265277	17.44710786766948
>500	0.006989244149644938	1.358915359663732
>1k	0.006989244149644938	6.203671045876864
>5k	0.0	0.0
>10k+	9.98463449949277E-4	5.065226203696151
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18104	4.913583463609519	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4167	1.130960135487233	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGT	3666	0.9949843668577384	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3619	0.9822281570262289	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCC	3343	0.9073193503560882	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	2874	0.780028660760813	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCT	2741	0.7439313010248394	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1763	0.47849357304151463	No Hit
GCAGGGATTGCTTTAGTGAAACAGCTTGTGTCTTTTATTTCGTTTATTGTG	835	0.22662628104915755	No Hit
CTCCACGGGGTAAGAAACCAATCAAGATCAAGGAAGGTGAAATAATCTATA	824	0.2236407851311447	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTT	823	0.22336937641132532	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCT	707	0.1918859649122807	No Hit
AAACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCT	603	0.16365945805106827	No Hit
AATTTTGGGATGTATGGGAAACCATGAACTGTACATGCGAAGGAGAAAGCC	542	0.1471035261420879	No Hit
AACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	523	0.14194676046552024	No Hit
AGCGCGGGAAAGCTATTTTTATTTTATGCCGACTTTTGATTATTTTGATGC	482	0.13081900295292687	No Hit
AGGAAAGGGATTTTTATTTACTTTTTTGTAAAGTGATTTCCAGTCTTGTGT	481	0.13054759423310752	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	475	0.1289191419141914	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTAT	474	0.12864773319437206	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	471	0.12783350703491403	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	443	0.12023406287997221	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTT	436	0.11833420184123675	No Hit
AAGCTGGGAAGAATATAGTTTGACTTGGGTCTGGGATGCTCTTCTTTTTTT	432	0.11724856696195936	No Hit
GCAGCAGGGATTTTTTATTTGCTTATTCCTTCATGTCGGACGAGGCTTATA	432	0.11724856696195936	No Hit
GGAGGAGGGGAGGAACTGGGAGGAGCTGGGGTAAGGAAAACTATAATTAGA	423	0.1148058884835852	No Hit
AAAGTTGGGGATGTATCTGGGGAACTGAGGAGAGCAGAACGGAAAGGTTCT	403	0.10937771408719821	No Hit
AAACTCGGGATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACA	372	0.10096404377279833	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7140871981935034E-4	0.0	0.0	0.9583441896821261	0.0
2	2.7140871981935034E-4	0.0	0.0	1.2137397950321347	0.0
3	2.7140871981935034E-4	0.0	0.0	1.6295379537953796	0.0
4	2.7140871981935034E-4	0.0	0.0	3.698215216258468	0.0
5	2.7140871981935034E-4	0.0	0.0	3.979394649991315	0.0
6	2.7140871981935034E-4	0.0	0.0	5.338609518846622	0.0
7	2.7140871981935034E-4	0.0	0.0	6.431301024839326	0.0
8	2.7140871981935034E-4	0.0	0.0	7.317721903769324	0.0
9	2.7140871981935034E-4	0.0	0.0	9.630395605350008	0.0
10	2.7140871981935034E-4	0.0	0.0	11.776967170401251	0.0
11	2.7140871981935034E-4	0.0	0.0	14.328209136703144	0.0
12	2.7140871981935034E-4	0.0	0.0	15.202416623241271	0.0
13	2.7140871981935034E-4	0.0	0.0	15.620928869202709	0.0
14	2.7140871981935034E-4	0.0	0.0	16.069567483064095	0.0
15	2.7140871981935034E-4	0.0	0.0	16.3936294945284	0.0
16	2.7140871981935034E-4	0.0	0.0	17.027640264026402	0.0
17	2.7140871981935034E-4	0.0	0.0	17.893162671530312	0.0
18	2.7140871981935034E-4	0.0	0.0	19.21302327601181	0.0
19	2.7140871981935034E-4	0.0	0.0	19.651348358520064	0.0
20	2.7140871981935034E-4	0.0	0.0	20.188194806322738	0.0
21	2.7140871981935034E-4	0.0	0.0	20.63411933298593	0.0
22	2.7140871981935034E-4	0.0	0.0	21.154952666319264	0.0
23	2.7140871981935034E-4	0.0	0.0	21.64484540559319	0.0
24	2.7140871981935034E-4	0.0	0.0	21.99197715824214	0.0
25	2.7140871981935034E-4	0.0	0.0	22.274785044293903	0.0
26	2.7140871981935034E-4	0.0	0.0	22.537780093798855	0.0
27	2.7140871981935034E-4	0.0	0.0	22.875141132534306	0.0
28	2.7140871981935034E-4	0.0	0.0	23.208702449192288	0.0
29	2.7140871981935034E-4	0.0	0.0	23.53385009553587	0.0
30	2.7140871981935034E-4	0.0	0.0	24.024828469689073	0.0
31	2.7140871981935034E-4	0.0	0.0	24.324735105089456	0.0
32	2.7140871981935034E-4	0.0	0.0	24.642554715997914	0.0
33	2.7140871981935034E-4	0.0	0.0	24.95901728330728	0.0
34	2.7140871981935034E-4	0.0	0.0	25.304520583637313	0.0
35	2.7140871981935034E-4	0.0	0.0	25.654095014764636	0.0
36	2.7140871981935034E-4	0.0	0.0	25.958072780962308	0.0
37	2.7140871981935034E-4	0.0	0.0	26.234095449018586	0.0
38	2.7140871981935034E-4	0.0	0.0	26.535359128018065	0.0
39	2.7140871981935034E-4	0.0	0.0	26.832008858780615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	70	0.0	45.000004	29
TGATCCG	35	1.2089185E-7	45.000004	25
GTGCATT	35	1.2089185E-7	45.000004	16
TCGCAGC	35	1.2089185E-7	45.000004	45
CGTATTT	35	1.2089185E-7	45.000004	17
AGCTTAG	35	1.2089185E-7	45.000004	36
TTGTACG	35	1.2089185E-7	45.000004	1
GACCACA	35	1.2089185E-7	45.000004	41
ATTCCCG	35	1.2089185E-7	45.000004	1
GCATTAT	35	1.2089185E-7	45.000004	18
TCGTATT	35	1.2089185E-7	45.000004	16
CTACAAC	35	1.2089185E-7	45.000004	35
GTAAGTC	35	1.2089185E-7	45.000004	39
TTGCACG	35	1.2089185E-7	45.000004	1
TAATGCG	35	1.2089185E-7	45.000004	1
AATACAT	35	1.2089185E-7	45.000004	31
TGACTAT	35	1.2089185E-7	45.000004	22
CTAGCAG	35	1.2089185E-7	45.000004	1
CGGGTTA	20	7.02704E-4	45.0	6
AGGTATT	20	7.02704E-4	45.0	35
>>END_MODULE