SRR3553426_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	779032
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	31537	4.048229084299489	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	27229	3.4952351122932046	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	23099	2.9650900091395473	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6812	0.8744185091241438	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	6734	0.8644060834471498	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	5380	0.6906006428490742	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	5002	0.6420788876451802	RNA PCR Primer, Index 38 (96% over 26bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT	4301	0.5520954209839903	No Hit
AACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	4108	0.527321085655018	RNA PCR Primer, Index 38 (95% over 24bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGTC	3339	0.42860883763439755	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1507	0.19344519865679458	No Hit
AATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	1354	0.17380544059807557	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1237	0.15878680208258455	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATG	1234	0.15840170878731555	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1194	0.1532671315170622	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1052	0.13503938220766284	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	1015	0.1302898982326785	No Hit
AATGAAACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	920	0.11809527721582681	No Hit
AATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	879	0.11283233551381716	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCGAGCTATGTCGTATGCCGT	872	0.1119337844915228	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATAG	35	1.2112469E-7	45.000004	1
AATTGCG	35	1.2112469E-7	45.000004	1
TACGAAC	35	1.2112469E-7	45.000004	34
ACTACGG	65	0.0	45.000004	2
TAAGCGG	35	1.2112469E-7	45.000004	2
AATACGC	35	1.2112469E-7	45.000004	42
CTTAACG	25	3.8896447E-5	45.0	3
GTCGCTA	20	7.031886E-4	45.0	13
CATTGCG	20	7.031886E-4	45.0	12
CGGTTAT	45	3.8380676E-10	45.0	30
ATTAACG	25	3.8896447E-5	45.0	1
GTCTAAC	20	7.031886E-4	45.0	1
TCGATTG	45	3.8380676E-10	45.0	21
CCGTCGA	85	0.0	45.0	40
CATTACG	25	3.8896447E-5	45.0	1
GTTACGC	20	7.031886E-4	45.0	35
AATGCGT	20	7.031886E-4	45.0	43
ACGGCTA	270	0.0	45.0	29
ATATATC	25	3.8896447E-5	45.0	34
CTAAACG	40	6.8102963E-9	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content