##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	779032
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.368960453485865	31.0	31.0	34.0	30.0	34.0
2	31.576933938528843	31.0	31.0	34.0	30.0	34.0
3	31.633179381591514	31.0	31.0	34.0	30.0	34.0
4	35.29602635065055	37.0	35.0	37.0	33.0	37.0
5	35.02055114552419	35.0	35.0	37.0	32.0	37.0
6	33.77388605346122	35.0	35.0	37.0	30.0	37.0
7	34.29180187720145	35.0	35.0	37.0	30.0	37.0
8	35.00418981505253	37.0	35.0	37.0	32.0	37.0
9	36.802508754454244	39.0	35.0	39.0	33.0	39.0
10	36.24436865237885	38.0	35.0	39.0	32.0	39.0
11	36.25642592345372	38.0	35.0	39.0	32.0	39.0
12	35.80933774222368	37.0	35.0	39.0	30.0	39.0
13	36.00151726758336	37.0	35.0	39.0	31.0	39.0
14	37.01463996344181	39.0	36.0	40.0	31.0	41.0
15	37.227103636307625	39.0	36.0	40.0	32.0	41.0
16	36.73443452900523	38.0	35.0	40.0	30.0	41.0
17	36.78803694841804	38.0	35.0	40.0	30.0	41.0
18	36.49915151110609	38.0	35.0	40.0	30.0	41.0
19	36.29147198061184	38.0	34.0	40.0	29.0	41.0
20	36.57899547130285	38.0	35.0	40.0	30.0	41.0
21	36.67358593741977	39.0	35.0	40.0	30.0	41.0
22	36.47263783772682	38.0	35.0	40.0	30.0	41.0
23	36.38537441337455	38.0	35.0	40.0	30.0	41.0
24	36.368160743076025	38.0	35.0	40.0	30.0	41.0
25	36.378325922426804	38.0	35.0	40.0	30.0	41.0
26	36.09344673902998	38.0	35.0	40.0	29.0	41.0
27	36.01239486953039	38.0	35.0	40.0	29.0	41.0
28	35.91255558179895	38.0	34.0	40.0	29.0	41.0
29	35.72385986711714	38.0	34.0	40.0	27.0	41.0
30	35.99877797060968	38.0	35.0	40.0	29.0	41.0
31	35.5984478172912	38.0	34.0	40.0	27.0	41.0
32	35.43931443124288	38.0	34.0	40.0	26.0	41.0
33	35.319199981515524	38.0	34.0	40.0	26.0	41.0
34	34.885805717865246	38.0	34.0	40.0	24.0	41.0
35	35.02428141591103	38.0	34.0	40.0	25.0	41.0
36	34.7780655993592	38.0	34.0	40.0	24.0	41.0
37	34.92215210671706	38.0	34.0	40.0	24.0	41.0
38	34.82550780969203	38.0	34.0	40.0	24.0	41.0
39	34.66710610090471	38.0	33.0	40.0	24.0	41.0
40	34.44981592540486	38.0	33.0	40.0	23.0	41.0
41	34.502994742192875	37.0	33.0	40.0	23.0	41.0
42	34.41586481684963	37.0	33.0	40.0	23.0	41.0
43	34.484113617925836	37.0	33.0	40.0	23.0	41.0
44	34.450978907156575	37.0	33.0	40.0	23.0	41.0
45	34.17365525421292	37.0	33.0	40.0	23.0	41.0
46	33.81427335462472	37.0	32.0	40.0	22.0	41.0
47	33.84463154273509	37.0	32.0	40.0	22.0	41.0
48	33.72838599698087	37.0	32.0	40.0	21.0	41.0
49	33.521642243194115	36.0	32.0	40.0	20.0	41.0
50	33.48217659865063	36.0	32.0	40.0	20.0	41.0
51	31.762658016615493	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	8.0
11	8.0
12	12.0
13	16.0
14	34.0
15	65.0
16	155.0
17	272.0
18	721.0
19	1796.0
20	4180.0
21	6570.0
22	8315.0
23	8698.0
24	8462.0
25	8762.0
26	9110.0
27	10285.0
28	12463.0
29	15958.0
30	21069.0
31	27743.0
32	36295.0
33	45857.0
34	60367.0
35	71407.0
36	78949.0
37	97368.0
38	126361.0
39	117701.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.430005442651908	46.714127275901376	14.962543258813502	21.893324022633216
2	17.816212941188553	45.49941465819119	16.99288347590343	19.691488924716825
3	17.6996580371538	24.534935663746804	36.1665502829152	21.598856016184186
4	33.68218507070313	24.792177984986495	16.066220643054457	25.459416301255917
5	15.568680105566909	43.778432721634026	16.700597664794255	23.952289508004807
6	18.420809414760882	32.65963400733218	30.9677907967837	17.951765781123243
7	65.51348339991169	21.839154232432044	7.636528409616036	5.010833958040235
8	67.3621879460664	5.317881678801385	5.349074235718174	21.970856139414042
9	73.28184208094147	7.86219308064367	9.822317953562882	9.03364688485197
10	39.40942092237546	26.82829460150546	16.968109140574455	16.794175335544626
11	26.30829028846055	24.072695344992248	23.045523162078066	26.573491204469136
12	27.433917990531842	21.14958050503702	35.124744554780804	16.291756949650335
13	23.180305815422216	30.909256615902812	22.758500292670906	23.151937276004066
14	17.483877427371404	24.067560767721993	35.245920578358785	23.20264122654782
15	16.679930991281488	28.534386263978888	22.652856365335442	32.132826379404186
16	18.703724622351842	30.706825907023074	30.957521642243197	19.63192782838189
17	17.250254161574876	27.343421066143627	27.54033210445784	27.865992667823658
18	18.34558785775167	28.760307663870034	24.12121710019614	28.772887378182155
19	22.026181209501022	28.03248133581162	31.516677106973784	18.424660347713573
20	19.869787120426373	36.92287351482352	24.706045451277998	18.501293913472104
21	19.256205136631102	24.547387013627166	31.242105587446982	24.954302262294746
22	23.094686739440743	31.390879963852576	25.560952566775175	19.953480729931506
23	22.79161831606404	28.00180223662186	22.139141909446593	27.067437537867505
24	16.610742562564823	40.06125550683412	22.99366393164851	20.334337998952545
25	19.82717012908327	23.9461280152805	30.448428305897572	25.77827354973865
26	26.375039792973844	29.865268692428554	20.328818328387026	23.43087318621058
27	18.365484344673902	28.954908142412634	29.908270777066924	22.771336735846536
28	26.913400219759907	24.78293574590004	25.644261082985036	22.659402951355016
29	18.73183643290648	37.633242280163074	25.0517308659978	18.583190420932645
30	26.26182236416476	23.77951098286078	21.77240472791875	28.18626192505571
31	27.09529261955863	28.99187709875846	21.344437712443135	22.568392569239776
32	23.89760626007661	24.236488359913327	24.10619846168065	27.759706918329414
33	28.282535248872957	27.058708756508075	22.226044629745633	22.43271136487333
34	21.41888908286181	27.91939227143429	27.030853674816953	23.630864970886947
35	32.32537302703869	26.5535947175469	22.65324145863071	18.4677907967837
36	20.16656568664702	28.437342753571098	27.922087924501177	23.474003635280706
37	25.456592283757278	24.74622351841773	25.643362531962744	24.15382166586225
38	23.049117366167245	29.73998500703437	23.604678626808656	23.606218999989732
39	18.483964715185	24.063838199201058	34.46379609566744	22.9884009899465
40	25.23349490136477	29.48582343215683	22.79636780003902	22.484313866439376
41	19.2470912619764	22.907916491235277	36.20262068823873	21.642371558549584
42	28.127984473038335	23.846902309532855	23.403531562246478	24.62158165518233
43	22.814723913780178	24.090666365438135	28.856324258823772	24.23828546195792
44	21.24585382885427	20.982706743753788	28.207698785159018	29.56374064223293
45	29.326009714620195	26.838948849341232	23.416368005422115	20.418673430616458
46	17.856391008328284	24.610799042914795	36.47411146140338	21.058698487353535
47	24.13585064541636	27.665872518715535	23.518417728668396	24.679859107199704
48	19.96413497776728	21.951858203514103	35.16440916419351	22.919597654525102
49	23.30135347456844	23.139357561691945	28.28150833341891	25.277780630320706
50	23.426765524394376	21.977659454297125	25.651321126731634	28.94425389457686
51	18.34186528923074	23.1242105587447	34.51924953018618	24.014674621838385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7870.0
1	5897.5
2	3925.0
3	2463.5
4	1002.0
5	910.5
6	819.0
7	923.0
8	1027.0
9	1097.5
10	1168.0
11	1253.5
12	1339.0
13	1458.5
14	1578.0
15	1621.0
16	1664.0
17	1767.0
18	1870.0
19	2144.5
20	2419.0
21	2299.5
22	2180.0
23	2774.0
24	3368.0
25	5019.0
26	6854.0
27	7038.0
28	7846.5
29	8655.0
30	12279.0
31	15903.0
32	16071.0
33	16239.0
34	17868.0
35	19497.0
36	20038.5
37	20580.0
38	23147.0
39	25714.0
40	25529.5
41	25345.0
42	28543.5
43	31742.0
44	72263.0
45	112784.0
46	100628.5
47	88473.0
48	75161.0
49	61849.0
50	55198.0
51	48547.0
52	45744.0
53	42941.0
54	40906.5
55	38872.0
56	36841.0
57	34810.0
58	32486.5
59	30163.0
60	28196.5
61	26230.0
62	23666.5
63	21103.0
64	20100.0
65	19097.0
66	16634.5
67	14172.0
68	12240.5
69	10309.0
70	9237.0
71	8165.0
72	6777.0
73	5389.0
74	4586.0
75	2988.5
76	2194.0
77	1619.5
78	1045.0
79	847.0
80	649.0
81	568.5
82	488.0
83	320.5
84	153.0
85	117.5
86	82.0
87	61.0
88	40.0
89	38.5
90	37.0
91	35.0
92	33.0
93	19.5
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	779032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.518955403151708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.227287343104	17.048884630489407
2	8.414890257020605	3.621592963264844
3	2.998094646001559	1.9354759494520635
4	1.5857389660394807	1.3649378436497395
5	0.9405890936572521	1.0120247379550646
6	0.6913667912259766	0.8926494688567133
7	0.46330109111517764	0.6978828862557324
8	0.3718126995458016	0.6400816719881239
9	0.2909009246464223	0.563389562178176
>10	3.2109786598888137	16.384813033385072
>50	1.1471787360129575	17.609669472986408
>100	0.6427583860546533	21.074561474794855
>500	0.0048327698199597995	0.7572220271079093
>1k	0.006040962274949749	2.644232318180598
>5k	0.0024163849099798997	3.110525092641726
>10k+	0.001812288682484925	10.642056866813562
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	31537	4.048229084299489	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	27229	3.4952351122932046	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	23099	2.9650900091395473	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6812	0.8744185091241438	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	6734	0.8644060834471498	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	5380	0.6906006428490742	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	5002	0.6420788876451802	RNA PCR Primer, Index 38 (96% over 26bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT	4301	0.5520954209839903	No Hit
AACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	4108	0.527321085655018	RNA PCR Primer, Index 38 (95% over 24bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGTC	3339	0.42860883763439755	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1507	0.19344519865679458	No Hit
AATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	1354	0.17380544059807557	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1237	0.15878680208258455	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATG	1234	0.15840170878731555	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1194	0.1532671315170622	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1052	0.13503938220766284	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	1015	0.1302898982326785	No Hit
AATGAAACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	920	0.11809527721582681	No Hit
AATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	879	0.11283233551381716	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCGAGCTATGTCGTATGCCGT	872	0.1119337844915228	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.80137914745479	0.0
2	0.0	0.0	0.0	0.8963688269544768	0.0
3	0.0	0.0	0.0	1.6398556156871604	0.0
4	0.0	0.0	0.0	5.409277154211893	0.0
5	0.0	0.0	0.0	5.675890078969799	0.0
6	0.0	0.0	0.0	6.960047854260159	0.0
7	0.0	0.0	0.0	7.960006777641997	0.0
8	0.0	0.0	0.0	8.18220560901221	0.0
9	0.0	0.0	0.0	13.009221700777376	0.0
10	0.0	0.0	0.0	13.488791217819037	0.0
11	0.0	0.0	0.0	17.929558734429396	0.0
12	0.0	0.0	0.0	18.323509175489583	0.0
13	0.0	0.0	0.0	18.64878464556013	0.0
14	0.0	0.0	0.0	19.434631696772406	0.0
15	0.0	0.0	0.0	19.696751866418836	0.0
16	0.0	0.0	0.0	19.961439324700397	0.0
17	0.0	0.0	0.0	20.203534642992842	0.0
18	0.0	0.0	0.0	21.14431756333501	0.0
19	0.0	0.0	0.0	21.368313496749813	0.0
20	0.0	0.0	0.0	21.531079596216845	0.0
21	0.0	0.0	0.0	21.742239086456014	0.0
22	0.0	0.0	0.0	21.92323293523244	0.0
23	0.0	0.0	0.0	22.0997340288974	0.0
24	0.0	0.0	0.0	22.233874860082768	0.0
25	0.0	0.0	0.0	22.35530761252426	0.0
26	0.0	0.0	0.0	22.47892256030561	0.0
27	0.0	0.0	0.0	22.60497643229033	0.0
28	0.0	0.0	0.0	22.728591380071677	0.0
29	0.0	0.0	0.0	22.847071750582774	0.0
30	0.0	0.0	0.0	23.02049209788558	0.0
31	0.0	0.0	0.0	23.140127748282485	0.0
32	0.0	0.0	0.0	23.265411433676665	0.0
33	0.0	0.0	0.0	23.40211955349716	0.0
34	0.0	0.0	0.0	23.525477772414998	0.0
35	0.0	0.0	0.0	23.661800798940224	0.0
36	0.0	0.0	0.0	23.784902288994548	0.0
37	0.0	0.0	0.0	23.91827293358938	0.0
38	0.0	0.0	0.0	24.043043161256534	0.0
39	0.0	0.0	0.0	24.17795417903244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATAG	35	1.2112469E-7	45.000004	1
AATTGCG	35	1.2112469E-7	45.000004	1
TACGAAC	35	1.2112469E-7	45.000004	34
ACTACGG	65	0.0	45.000004	2
TAAGCGG	35	1.2112469E-7	45.000004	2
AATACGC	35	1.2112469E-7	45.000004	42
CTTAACG	25	3.8896447E-5	45.0	3
GTCGCTA	20	7.031886E-4	45.0	13
CATTGCG	20	7.031886E-4	45.0	12
CGGTTAT	45	3.8380676E-10	45.0	30
ATTAACG	25	3.8896447E-5	45.0	1
GTCTAAC	20	7.031886E-4	45.0	1
TCGATTG	45	3.8380676E-10	45.0	21
CCGTCGA	85	0.0	45.0	40
CATTACG	25	3.8896447E-5	45.0	1
GTTACGC	20	7.031886E-4	45.0	35
AATGCGT	20	7.031886E-4	45.0	43
ACGGCTA	270	0.0	45.0	29
ATATATC	25	3.8896447E-5	45.0	34
CTAAACG	40	6.8102963E-9	45.0	1
>>END_MODULE