##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651221
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5831537987872	31.0	31.0	34.0	30.0	34.0
2	31.76513963769596	31.0	31.0	34.0	30.0	34.0
3	31.937221004850887	33.0	31.0	34.0	30.0	34.0
4	35.68185147591985	37.0	35.0	37.0	33.0	37.0
5	35.42110128512441	37.0	35.0	37.0	33.0	37.0
6	34.03519388963194	37.0	35.0	37.0	32.0	37.0
7	34.859619084765384	37.0	35.0	37.0	32.0	37.0
8	35.553534053723695	37.0	35.0	37.0	32.0	37.0
9	37.11723976960202	39.0	37.0	39.0	34.0	39.0
10	36.58448207290613	38.0	35.0	39.0	32.0	39.0
11	36.70432003881939	39.0	35.0	39.0	32.0	39.0
12	36.670382251186616	39.0	35.0	39.0	32.0	39.0
13	36.62613459946777	39.0	35.0	39.0	32.0	39.0
14	37.739556924607776	39.0	37.0	41.0	33.0	41.0
15	37.788280783328545	39.0	37.0	41.0	33.0	41.0
16	37.680990017213816	39.0	36.0	41.0	33.0	41.0
17	37.61269062269184	39.0	36.0	41.0	32.0	41.0
18	37.50190488328847	39.0	36.0	41.0	32.0	41.0
19	37.44597149047712	39.0	36.0	41.0	32.0	41.0
20	37.3356771357189	39.0	36.0	41.0	32.0	41.0
21	37.26907301822269	39.0	35.0	40.0	32.0	41.0
22	37.175841995267355	39.0	35.0	40.0	31.0	41.0
23	37.04186290061285	39.0	35.0	40.0	31.0	41.0
24	36.88556112287534	39.0	35.0	40.0	31.0	41.0
25	36.83089918783332	39.0	35.0	40.0	31.0	41.0
26	36.823939952796366	39.0	35.0	40.0	31.0	41.0
27	36.80734036525235	39.0	35.0	40.0	31.0	41.0
28	36.52965122439233	39.0	35.0	40.0	30.0	41.0
29	36.30841296579809	39.0	35.0	40.0	30.0	41.0
30	36.33777934065394	39.0	35.0	40.0	30.0	41.0
31	36.254253164440335	38.0	35.0	40.0	30.0	41.0
32	36.20388623831234	38.0	35.0	40.0	30.0	41.0
33	36.17364612013433	38.0	35.0	40.0	30.0	41.0
34	36.082778350206766	38.0	35.0	40.0	30.0	41.0
35	35.98014652475888	38.0	35.0	40.0	29.0	41.0
36	35.93616913459486	38.0	35.0	40.0	29.0	41.0
37	35.88400404778101	38.0	35.0	40.0	29.0	41.0
38	35.775182311381236	38.0	35.0	40.0	28.0	41.0
39	35.704470525366965	38.0	35.0	40.0	28.0	41.0
40	35.54807507743147	38.0	34.0	40.0	27.0	41.0
41	35.41704429064787	38.0	34.0	40.0	27.0	41.0
42	35.328386216046475	38.0	34.0	40.0	27.0	41.0
43	35.19703602924353	38.0	34.0	40.0	26.0	41.0
44	35.086780063910716	38.0	34.0	40.0	26.0	41.0
45	34.95639882620493	38.0	34.0	40.0	26.0	41.0
46	34.76880966676443	37.0	33.0	40.0	24.0	41.0
47	34.70755703516932	37.0	33.0	40.0	24.0	41.0
48	34.608857822459655	37.0	33.0	40.0	24.0	41.0
49	34.40154110509336	37.0	33.0	40.0	24.0	41.0
50	34.259553976299905	37.0	33.0	40.0	23.0	41.0
51	33.03564995600572	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	8.0
11	9.0
12	10.0
13	23.0
14	28.0
15	46.0
16	96.0
17	200.0
18	404.0
19	918.0
20	1672.0
21	2624.0
22	3382.0
23	3966.0
24	4874.0
25	5499.0
26	6516.0
27	7435.0
28	9267.0
29	11494.0
30	14693.0
31	18697.0
32	24192.0
33	32093.0
34	46308.0
35	60730.0
36	65648.0
37	87416.0
38	121946.0
39	121009.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.489389930607278	31.81531308111993	20.793248374975622	27.902048613297175
2	21.323022445529244	34.16336389643455	21.550134286210056	22.963479371826153
3	20.404900947604578	32.473768505622516	20.292036036921413	26.829294509851493
4	19.91981216821939	33.51427549173015	20.503331434336424	26.062580905714032
5	17.536289523832924	36.82820425017007	17.214432581258894	28.421073644738115
6	18.117966097530637	41.338193946448285	18.391605921799208	22.15223403422187
7	82.10100718496486	9.406791242911392	4.792689424941763	3.699512147181986
8	83.34405677949574	8.538115324905062	3.9676238941926014	4.150204001406588
9	75.99785633448552	12.344964305512262	7.3924213131947525	4.264758046807458
10	30.441585882519146	42.26737159888886	12.757727407439257	14.533315111152742
11	22.83925119122387	32.16757444861268	26.369389193530306	18.623785166633137
12	24.726475344007643	26.914519034244904	30.400278860786123	17.958726760961333
13	24.95604410791421	26.972870960856604	27.364135984558235	20.706948946670945
14	18.923990473280192	31.646246051647598	28.248935461233593	21.18082801383862
15	18.475294869176516	31.913129337045337	30.28818173861101	19.32339405516714
16	20.332268154743165	31.97639511010855	26.263741494822803	21.42759524032548
17	19.54682051100932	29.485228516893653	27.915868806442052	23.052082165654976
18	22.924782831020497	29.216963212181422	26.398104483731327	21.460149473066746
19	22.259417309945473	31.583440951689212	26.278329476475726	19.87881226188959
20	21.90515969233179	30.369567320464174	25.54186673955539	22.183406247648648
21	24.18641290744617	31.31886103181562	25.778192042332787	18.716534018405426
22	20.787413182314452	31.621830377091648	25.33118557294682	22.25957086764708
23	19.37299319278709	30.85296696513165	25.233983547827847	24.54005629425341
24	18.932129031465507	31.250220739196067	26.63258709408941	23.185063135249017
25	20.145234874182496	32.91079372440385	24.897231508197677	22.046739893215975
26	20.47999066369174	31.22841554556748	25.03666190125933	23.25493188948145
27	18.94349230138463	29.277004273510837	27.299027519075707	24.48047590602883
28	20.440065661273206	30.963067837185843	27.100170295491083	21.49669620604987
29	21.51819428427523	32.03766463305084	25.32289345705989	21.12124762561404
30	21.407786296817825	31.031401014402178	25.80122569757425	21.75958699120575
31	20.95709444259322	31.73914846112149	24.297895798814842	23.005861297470446
32	21.65271083088537	30.8253265788419	25.06507007605713	22.456892514215603
33	20.750559333928116	28.8777542493255	27.284439537422777	23.087246879323608
34	19.909677359913147	29.453288514958825	27.184627031376447	23.452407093751585
35	20.43284844929755	28.498313168647815	28.625458945580682	22.443379436473947
36	20.77128962364543	28.797443571383603	28.518122112155474	21.913144692815496
37	19.503824354558592	30.778491479850928	25.829480314670445	23.888203850920043
38	21.745306124956045	29.289903120446052	26.85125326118169	22.113537493416214
39	22.17020028531021	27.258027612745906	26.298752650789826	24.273019451154063
40	23.595522871651866	27.927385634062784	24.997197571945623	23.479893922339727
41	18.4845083312731	27.172649530650883	28.251238826757735	26.09160331131828
42	21.265898980530416	28.88220742267218	26.78414854557823	23.06774505121917
43	19.57031483935561	27.950879962409076	28.25937738494305	24.219427813292263
44	21.629370060240685	26.50697689417264	26.988073173315968	24.875579872270702
45	21.911916231202618	27.55070859201408	27.674015426406704	22.8633597503766
46	19.874666203946127	28.375313449658414	29.65951650822071	22.09050383817475
47	20.433616237805598	28.270740654862177	28.028580159423605	23.26706294790862
48	20.670248655986217	26.945537689970074	28.492631533688257	23.891582120355455
49	20.067534677167966	27.473622625805987	29.093042146982363	23.365800550043687
50	20.237983725954784	26.92772499658334	28.317422196151536	24.51686908131034
51	20.80752924122533	26.14273802595432	28.070962085067897	24.97877064775245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15490.0
1	11581.0
2	7672.0
3	4781.5
4	1891.0
5	1584.0
6	1277.0
7	1383.5
8	1490.0
9	1595.5
10	1701.0
11	1878.5
12	2056.0
13	2072.5
14	2089.0
15	2040.5
16	1992.0
17	2075.5
18	2159.0
19	2461.0
20	2763.0
21	2827.0
22	2891.0
23	3510.0
24	4129.0
25	4407.0
26	5521.0
27	6357.0
28	9396.5
29	12436.0
30	13101.0
31	13766.0
32	14949.5
33	16133.0
34	17623.0
35	19113.0
36	21089.0
37	23065.0
38	24280.0
39	25495.0
40	27907.0
41	30319.0
42	32271.0
43	34223.0
44	37173.0
45	40123.0
46	43809.0
47	47495.0
48	50787.5
49	54080.0
50	52912.0
51	51744.0
52	46738.0
53	41732.0
54	38110.0
55	34488.0
56	31448.0
57	28408.0
58	27064.5
59	25721.0
60	24782.0
61	23843.0
62	21816.5
63	19790.0
64	17570.0
65	15350.0
66	13207.0
67	11064.0
68	8984.5
69	6905.0
70	6014.0
71	5123.0
72	4768.5
73	4414.0
74	3780.0
75	2577.0
76	2008.0
77	1557.5
78	1107.0
79	865.5
80	624.0
81	509.5
82	395.0
83	274.0
84	153.0
85	145.0
86	137.0
87	108.0
88	79.0
89	54.5
90	30.0
91	27.0
92	24.0
93	28.0
94	32.0
95	22.5
96	13.0
97	6.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.663163169596558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.62917439203736	19.922101691439213
2	9.410179534134905	4.829899456794044
3	3.5429257501487426	2.7276804487149744
4	1.8508017804404542	1.899897123440928
5	1.1272488725640608	1.4464385874677623
6	0.732417392473411	1.1277688230777354
7	0.5347172181353659	0.9605774653020455
8	0.3980587161294655	0.8172356626488471
9	0.3025198291306196	0.6987254163315806
>10	2.8373643802236703	16.537493203065576
>50	0.9704824281473928	17.95905091717143
>100	0.6538368538328013	26.035087518155287
>500	0.005438569024487258	0.8578957020689113
>1k	0.004229998130156756	2.1461349640151237
>5k	0.0	0.0
>10k+	6.042854471652509E-4	2.0340130203065514
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13116	2.0140628143134203	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3137	0.48171050994977127	No Hit
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	3045	0.46758320140167475	TruSeq Adapter, Index 20 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2353	0.3613212718877309	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1646	0.2527559768496409	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGT	1403	0.21544145535847278	No Hit
AATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT	1192	0.18304078031881652	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCC	1063	0.16323183681115935	No Hit
TATTACGGACTGCACTGGCTGCTTGGGCTTTTGTGAGGGGTCGGACTGCAA	803	0.12330683439262555	No Hit
TCTTATGGGACAACTGTTTAATCAGTTTTATTTTTGTACAGTATTAGTGAC	664	0.10196231386887093	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5564931106337173	0.0
2	0.0	0.0	0.0	0.7716274505889705	0.0
3	0.0	0.0	0.0	0.987836694455492	0.0
4	0.0	0.0	0.0	2.0495346433852717	0.0
5	0.0	0.0	0.0	2.215223403422187	0.0
6	0.0	0.0	0.0	3.0452027806228608	0.0
7	0.0	0.0	0.0	3.7021226281093513	0.0
8	0.0	0.0	0.0	4.241570833864387	0.0
9	0.0	0.0	0.0	5.248295125617878	0.0
10	0.0	0.0	0.0	6.618951170186465	0.0
11	0.0	0.0	0.0	7.726869987300778	0.0
12	0.0	0.0	0.0	8.356917237005563	0.0
13	0.0	0.0	0.0	8.662189947805738	0.0
14	0.0	0.0	0.0	8.95686717719484	0.0
15	0.0	0.0	0.0	9.18520747948853	0.0
16	0.0	0.0	0.0	9.64296298798718	0.0
17	0.0	0.0	0.0	10.238920427934604	0.0
18	0.0	0.0	0.0	11.154585002633514	0.0
19	0.0	0.0	0.0	11.436977615893836	0.0
20	0.0	0.0	0.0	11.90548216350517	0.0
21	0.0	0.0	0.0	12.25467237696573	0.0
22	0.0	0.0	0.0	12.643173362038386	0.0
23	0.0	0.0	0.0	13.029370981586895	0.0
24	0.0	0.0	0.0	13.302550132750634	0.0
25	0.0	0.0	0.0	13.534422262181348	0.0
26	0.0	0.0	0.0	13.741418043951285	0.0
27	0.0	0.0	0.0	14.072334890920287	0.0
28	0.0	0.0	0.0	14.35288481176129	0.0
29	0.0	0.0	0.0	14.60057338445781	0.0
30	0.0	0.0	0.0	15.008115524530075	0.0
31	0.0	0.0	0.0	15.258568135855569	0.0
32	0.0	0.0	0.0	15.5300581523016	0.0
33	0.0	0.0	0.0	15.812450765561922	0.0
34	0.0	0.0	0.0	16.086858378338537	0.0
35	0.0	0.0	0.0	16.36848320309081	0.0
36	0.0	0.0	0.0	16.605883409779477	0.0
37	0.0	0.0	0.0	16.85096150154863	0.0
38	0.0	0.0	0.0	17.0978822857371	0.0
39	0.0	0.0	0.0	17.391023938110106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	40	6.8066583E-9	45.0	42
CGGACAA	25	3.8889368E-5	45.0	27
TCACGCC	20	7.0310326E-4	45.0	43
CTTCGAT	35	1.2108467E-7	45.0	32
AGCGTAA	20	7.0310326E-4	45.0	44
ACGCCTC	20	7.0310326E-4	45.0	45
CGAGTTA	25	3.8889368E-5	45.0	33
AATCACG	20	7.0310326E-4	45.0	1
CGAAATT	35	1.2108467E-7	45.0	13
CTCCGAT	20	7.0310326E-4	45.0	44
ATAGGCG	20	7.0310326E-4	45.0	1
AGATACG	25	3.8889368E-5	45.0	1
CGACGAG	25	3.8889368E-5	45.0	1
ACGTTAG	20	7.0310326E-4	45.0	1
TCACACG	25	3.8889368E-5	45.0	1
CGGTTGA	50	2.1827873E-11	45.0	31
CGGCACG	20	7.0310326E-4	45.0	1
GCACGTC	20	7.0310326E-4	45.0	11
ACTTCGC	25	3.8889368E-5	45.0	29
TTCGTAG	40	6.8066583E-9	45.0	1
>>END_MODULE