##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424001
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.622543343058155	31.0	31.0	34.0	30.0	34.0
2	31.84292961573204	33.0	31.0	34.0	30.0	34.0
3	32.001549524647345	33.0	31.0	34.0	30.0	34.0
4	35.71673180016085	37.0	35.0	37.0	33.0	37.0
5	35.46886210174033	37.0	35.0	37.0	33.0	37.0
6	34.00667451256011	37.0	35.0	37.0	32.0	37.0
7	34.913858693729495	37.0	35.0	37.0	32.0	37.0
8	35.6578899578067	37.0	35.0	37.0	33.0	37.0
9	37.2995370293938	39.0	37.0	39.0	34.0	39.0
10	36.68625073997467	38.0	36.0	39.0	32.0	39.0
11	36.75512557753402	39.0	37.0	39.0	32.0	39.0
12	36.85103572868932	39.0	37.0	39.0	33.0	39.0
13	36.81536600149528	39.0	37.0	39.0	33.0	39.0
14	37.978459956462366	40.0	37.0	41.0	33.0	41.0
15	38.00318631323983	40.0	37.0	41.0	33.0	41.0
16	37.90804502819569	40.0	37.0	41.0	33.0	41.0
17	37.8664059754576	39.0	37.0	41.0	33.0	41.0
18	37.74876238499437	39.0	37.0	41.0	33.0	41.0
19	37.728889790354266	39.0	36.0	41.0	33.0	41.0
20	37.64037113120016	39.0	36.0	41.0	32.0	41.0
21	37.61403392916526	39.0	36.0	41.0	32.0	41.0
22	37.536576564677915	39.0	36.0	41.0	32.0	41.0
23	37.398815097134204	39.0	36.0	41.0	32.0	41.0
24	37.30085070554079	39.0	36.0	41.0	32.0	41.0
25	37.25834137183639	39.0	36.0	41.0	32.0	41.0
26	37.252723460557874	39.0	36.0	41.0	32.0	41.0
27	37.208775451001294	39.0	36.0	41.0	32.0	41.0
28	37.018249956957646	39.0	36.0	41.0	31.0	41.0
29	36.84908526159136	39.0	35.0	40.0	31.0	41.0
30	36.91734217607977	39.0	35.0	40.0	31.0	41.0
31	36.85411826858899	39.0	35.0	40.0	31.0	41.0
32	36.770153843976786	39.0	35.0	40.0	31.0	41.0
33	36.70063749849646	39.0	35.0	40.0	30.0	41.0
34	36.66850078183778	39.0	35.0	40.0	30.0	41.0
35	36.555328878941324	39.0	35.0	40.0	30.0	41.0
36	36.524404423574474	39.0	35.0	40.0	30.0	41.0
37	36.453932891667705	39.0	35.0	40.0	30.0	41.0
38	36.3295015813642	39.0	35.0	40.0	30.0	41.0
39	36.29367619415992	39.0	35.0	40.0	30.0	41.0
40	36.17053497515336	39.0	35.0	40.0	30.0	41.0
41	36.07630878229061	39.0	35.0	40.0	30.0	41.0
42	35.970849125355834	38.0	35.0	40.0	29.0	41.0
43	35.909285591307565	38.0	35.0	40.0	29.0	41.0
44	35.81130704880413	38.0	35.0	40.0	28.0	41.0
45	35.67327671397001	38.0	35.0	40.0	28.0	41.0
46	35.51370869408327	38.0	34.0	40.0	27.0	41.0
47	35.462074381900045	38.0	34.0	40.0	27.0	41.0
48	35.37053450345636	38.0	34.0	40.0	27.0	41.0
49	35.21982259475803	38.0	34.0	40.0	27.0	41.0
50	35.075304067679085	38.0	34.0	40.0	26.0	41.0
51	33.91842943766642	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	6.0
12	5.0
13	6.0
14	7.0
15	9.0
16	16.0
17	82.0
18	159.0
19	429.0
20	822.0
21	1381.0
22	1834.0
23	2155.0
24	2468.0
25	2702.0
26	3175.0
27	3821.0
28	4789.0
29	6232.0
30	8066.0
31	10756.0
32	13992.0
33	18734.0
34	26859.0
35	36697.0
36	42615.0
37	59199.0
38	85374.0
39	91596.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.110096438451794	32.516432744262396	22.517399723113858	25.856071094171945
2	23.98107551633133	31.727991207567907	20.077075289916767	24.213857986183996
3	21.40089292242235	32.06454701757779	21.5862698437032	24.94829021629666
4	19.958207645736685	33.80770328371867	19.27023757019441	26.963851500350234
5	17.385336355338783	37.07562010466957	18.6487767717529	26.890266768238753
6	17.414109872382376	43.639519718113874	18.19594765106686	20.75042275843689
7	86.2113532751102	7.736538357220855	3.240794243409803	2.811314124259141
8	87.34413362232635	5.879938962408107	2.901879948396348	3.874047466869182
9	80.35169728373283	11.237945193525487	5.268619649481958	3.1417378732597325
10	29.153704826167864	48.31545208619791	10.514597842929616	12.016245244704612
11	18.1504288904979	30.93223836736234	32.99614859398917	17.921184148150594
12	23.169992523602538	24.182254287136114	34.38789059459765	18.25986259466369
13	24.428951818509862	26.461965891589877	29.924221876835194	19.184860413065064
14	19.365520364338764	31.195916990761813	28.977761844901313	20.460800799998115
15	16.47283850745635	32.65558335947321	30.936247791868414	19.935330341202025
16	20.014575437322083	32.083179049106015	28.172810913181806	19.729434600390093
17	19.34382230230589	29.189317949721815	28.404649989033043	23.062209758939247
18	20.42848955544916	29.779646746116168	28.8419131086955	20.949950589739174
19	20.30136721375657	31.737660996082557	29.18271419171181	18.77825759844906
20	21.164101028063616	31.776575998641515	27.34993549543515	19.70938747785972
21	22.664097490336108	30.33058884295084	27.920453017799485	19.084860648913565
22	19.997830193796712	30.303230416909393	27.664557394911803	22.034381994382088
23	18.794059447972998	32.18600899526181	27.47210501862024	21.547826538144957
24	16.890054504588434	32.578932596857086	29.06007297152601	21.470939927028475
25	18.86056872507376	33.436949441156976	26.882955464727676	20.819526369041583
26	20.23650887615831	31.88553800580659	25.664090414881098	22.213862703154003
27	17.840523961028392	32.07563189709458	26.88248376772696	23.20136037415006
28	19.105143619944293	31.45134091664878	29.849457902221925	19.594057561184997
29	18.870946059089484	33.69378845804609	26.50064504564848	20.93462043721595
30	19.451369218468827	31.910537946844464	27.58342551078889	21.05466732389782
31	22.17093827608897	30.70936153452468	25.61196789630213	21.507732293084214
32	20.72117754439258	31.72516102556362	26.134136476093218	21.41952495395058
33	19.032030584833525	30.792616055150813	28.295687981867967	21.879665378147692
34	19.89122667163521	30.486248853186666	28.30842380088726	21.314100674290863
35	19.211982990606156	30.156532649687147	29.378940144009096	21.252544215697604
36	22.02211787236351	30.069740401555656	28.335310529927995	19.57283119615284
37	20.44240461697024	33.05746920408207	25.543335982698157	20.956790196249536
38	21.298770521767636	31.80157593967939	27.170926483663955	19.728727054889024
39	20.663158813304687	29.635071615397134	26.696399300945046	23.005370270353136
40	23.32871856434301	29.57563779330709	26.288617243827257	20.807026398522645
41	21.245704609187243	27.33979400991979	29.533892608743844	21.88060877214912
42	21.370940162876977	30.170447711208226	26.884842252730536	21.57376987318426
43	20.50773465156922	29.941202968860924	28.02658484296028	21.524477536609584
44	22.023532963365653	28.452055537604863	26.978474107372392	22.545937391657095
45	21.76881658297976	29.625401826882484	27.422576833545204	21.183204756592556
46	20.091697896938925	29.8551182662305	29.140733158648214	20.912450678182363
47	20.036745196355668	30.061249855542794	27.984603809896676	21.917401138204863
48	20.378253824873056	29.610071674359258	28.440734809587713	21.570939691179973
49	21.36716658687126	29.398751418039108	27.63106690786107	21.60301508722857
50	20.739809575920813	28.042386692484218	28.420451838556986	22.797351893037987
51	21.133204874516807	26.89569128374697	28.711488887997906	23.259614953738318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7063.0
1	5274.0
2	3485.0
3	2220.0
4	955.0
5	840.0
6	725.0
7	752.0
8	779.0
9	951.0
10	1123.0
11	1298.5
12	1474.0
13	1520.0
14	1566.0
15	1512.5
16	1459.0
17	1538.5
18	1618.0
19	1636.0
20	1654.0
21	1977.5
22	2301.0
23	2806.0
24	3311.0
25	3780.5
26	5791.5
27	7333.0
28	7138.5
29	6944.0
30	8728.5
31	10513.0
32	11849.0
33	13185.0
34	14127.0
35	15069.0
36	16969.5
37	18870.0
38	19719.5
39	20569.0
40	20597.5
41	20626.0
42	22704.5
43	24783.0
44	28852.5
45	32922.0
46	35263.0
47	37604.0
48	37391.0
49	37178.0
50	36020.5
51	34863.0
52	31177.5
53	27492.0
54	24317.0
55	21142.0
56	18584.0
57	16026.0
58	14092.5
59	12159.0
60	11474.0
61	10789.0
62	9608.5
63	8428.0
64	6908.0
65	5388.0
66	4450.5
67	3513.0
68	2929.5
69	2346.0
70	2069.0
71	1792.0
72	1442.5
73	1093.0
74	935.0
75	564.5
76	352.0
77	271.0
78	190.0
79	180.0
80	170.0
81	109.0
82	48.0
83	47.5
84	47.0
85	31.0
86	15.0
87	9.0
88	3.0
89	5.0
90	7.0
91	3.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	424001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.94148635983127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57263014043838	21.39553100605464
2	9.18140174341895	5.130840231557433
3	3.619564457482171	3.0340803275180446
4	1.87653619477529	2.097328419601738
5	1.232079988235779	1.7213073092755549
6	0.8704127254155166	1.459237517677273
7	0.6150318911606137	1.2029433638407865
8	0.47335863440890796	1.0581075061315888
9	0.3783469890032676	0.951441950926623
>10	3.9005354599475317	24.608773797113063
>50	0.8788505232864818	17.267930127814708
>100	0.39107151663616607	16.084909451805824
>500	0.0033932452636543696	0.5617629737810168
>1k	0.005938179211395147	1.9806292864616777
>5k	8.483113159135924E-4	1.4451767304400251
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6097	1.437968306678522	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1384	0.32641432449451774	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCC	1352	0.31886717248308377	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGT	1258	0.29669741344949657	No Hit
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT	1229	0.28985780693913454	Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1055	0.24882016787696254	No Hit
GTTATAGGGGTTTGTTGTTAGTTTTATTTTCACTTGACTGTTAAATGTTTT	1043	0.24598998587267484	No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT	1035	0.24410319786981635	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	689	0.1624996167461869	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	575	0.13561288770545352	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	569	0.13419779670330967	No Hit
CGGCTAGGGAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTC	537	0.12665064469187573	No Hit
GATGCCGGGGGTAATATTGTAGAATCAGGCAAAAAGCAAAGCAAAGCAAAA	469	0.11061294666757861	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4004707536067132	0.0
2	0.0	0.0	0.0	0.5066025787675029	0.0
3	0.0	0.0	0.0	0.6714606805172629	0.0
4	0.0	0.0	0.0	1.5096662507871443	0.0
5	0.0	0.0	0.0	1.625703712962941	0.0
6	0.0	0.0	0.0	2.3129662430041438	0.0
7	0.0	0.0	0.0	2.8848988563706217	0.0
8	0.0	0.0	0.0	3.560368961393959	0.0
9	0.0	0.0	0.0	4.61343251548935	0.0
10	0.0	0.0	0.0	5.840080565847722	0.0
11	0.0	0.0	0.0	7.040077735665718	0.0
12	0.0	0.0	0.0	7.6105952580300515	0.0
13	0.0	0.0	0.0	7.870264456923451	0.0
14	0.0	0.0	0.0	8.133707231822566	0.0
15	0.0	0.0	0.0	8.341961457638071	0.0
16	0.0	0.0	0.0	8.762715182275514	0.0
17	0.0	0.0	0.0	9.330638371135917	0.0
18	0.0	0.0	0.0	10.259173917042649	0.0
19	0.0	0.0	0.0	10.553276996988215	0.0
20	2.3584850035731047E-4	0.0	0.0	10.971908085122442	0.0
21	2.3584850035731047E-4	0.0	0.0	11.303511076624819	0.0
22	2.3584850035731047E-4	0.0	0.0	11.682047919698302	0.0
23	2.3584850035731047E-4	0.0	0.0	12.043839519246417	0.0
24	2.3584850035731047E-4	0.0	0.0	12.312235112653036	0.0
25	2.3584850035731047E-4	0.0	0.0	12.540064763998197	0.0
26	2.3584850035731047E-4	0.0	0.0	12.764828384838715	0.0
27	2.3584850035731047E-4	0.0	0.0	13.082280466319656	0.0
28	2.3584850035731047E-4	0.0	0.0	13.38086466777201	0.0
29	2.3584850035731047E-4	0.0	0.0	13.633930108655404	0.0
30	2.3584850035731047E-4	0.0	0.0	14.07803283482822	0.0
31	2.3584850035731047E-4	0.0	0.0	14.337230336720904	0.0
32	2.3584850035731047E-4	0.0	0.0	14.591946717106799	0.0
33	2.3584850035731047E-4	0.0	0.0	14.856097037506988	0.0
34	2.3584850035731047E-4	0.0	0.0	15.088879507359653	0.0
35	2.3584850035731047E-4	0.0	0.0	15.372841101789854	0.0
36	2.3584850035731047E-4	0.0	0.0	15.62213296666753	0.0
37	2.3584850035731047E-4	0.0	0.0	15.883217256563075	0.0
38	2.3584850035731047E-4	0.0	0.0	16.15349963797255	0.0
39	2.3584850035731047E-4	0.0	0.0	16.412461291364878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	30	2.162249E-6	45.000004	1
GTACCGC	35	1.2095006E-7	45.000004	40
ATAGCGG	35	1.2095006E-7	45.000004	2
CCGGAAG	35	1.2095006E-7	45.000004	1
ATTCGGA	30	2.162249E-6	45.000004	38
TACGGGT	30	2.162249E-6	45.000004	4
ATTCCGG	30	2.162249E-6	45.000004	2
CTACGCG	35	1.2095006E-7	45.000004	1
TATTCGG	30	2.162249E-6	45.000004	37
ACTACGG	70	0.0	45.000004	2
GAACCGC	30	2.162249E-6	45.000004	9
TAGTGCG	35	1.2095006E-7	45.000004	1
TATACCG	30	2.162249E-6	45.000004	1
TTATTCG	30	2.162249E-6	45.000004	36
TTCGAGA	35	1.2095006E-7	45.000004	35
GTTATCG	35	1.2095006E-7	45.000004	32
TAGTACG	30	2.162249E-6	45.000004	1
TAGTACC	35	1.2095006E-7	45.000004	38
AACACGT	30	2.162249E-6	45.000004	41
TCGCCTT	25	3.8866274E-5	45.0	43
>>END_MODULE