FastQCFastQC Report
Sat 18 Jun 2016
SRR3553415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762201
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131561.7260538886724106No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28450.3732611214102317No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26400.3463653288305841No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16820.22067669814130392No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT15210.19955366104216604TruSeq Adapter, Index 14 (95% over 22bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9110.11952227824419018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT551.8189894E-1245.00000420
ATCACGC302.1645355E-645.00000442
CGTAGTG302.1645355E-645.00000428
GAAACGT302.1645355E-645.00000435
GCGATTG551.8189894E-1245.0000041
AATTCGC302.1645355E-645.00000439
TTAACCG302.1645355E-645.0000041
CACGTTA302.1645355E-645.00000438
CACGTCG302.1645355E-645.00000436
TCGCCTA207.03179E-445.014
CTTAACG207.03179E-445.01
CTTCGAA253.8895654E-545.040
TCGTTTA351.2112105E-745.038
TCACCGA253.8895654E-545.038
CGAGTAT207.03179E-445.034
CATGCGT351.2112105E-745.034
CGTGATA502.1827873E-1145.014
CGCATAG207.03179E-445.01
GGTCCAA453.8380676E-1045.010
CACGCGA207.03179E-445.011