##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762201
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65004769083221	31.0	31.0	34.0	30.0	34.0
2	31.853381194724225	33.0	31.0	34.0	30.0	34.0
3	32.023363915817484	33.0	31.0	34.0	30.0	34.0
4	35.75429840685069	37.0	35.0	37.0	35.0	37.0
5	35.52148842628126	37.0	35.0	37.0	33.0	37.0
6	32.79089111664771	37.0	35.0	37.0	26.0	37.0
7	34.27358531411006	37.0	35.0	37.0	28.0	37.0
8	35.35057288038195	37.0	35.0	37.0	32.0	37.0
9	37.183088188023895	39.0	37.0	39.0	34.0	39.0
10	36.70170073248395	39.0	37.0	39.0	32.0	39.0
11	36.74938894071249	39.0	37.0	39.0	32.0	39.0
12	36.684964989550004	39.0	35.0	39.0	32.0	39.0
13	36.67066036386727	39.0	35.0	39.0	32.0	39.0
14	37.82777771217828	40.0	37.0	41.0	33.0	41.0
15	37.87204556278462	40.0	37.0	41.0	33.0	41.0
16	37.820382025213824	40.0	37.0	41.0	33.0	41.0
17	37.77221100470873	39.0	37.0	41.0	33.0	41.0
18	37.6357561850483	39.0	36.0	41.0	33.0	41.0
19	37.632758288168084	39.0	36.0	41.0	33.0	41.0
20	37.511510743229145	39.0	36.0	41.0	32.0	41.0
21	37.4576430626567	39.0	36.0	41.0	32.0	41.0
22	37.38940253292767	39.0	36.0	41.0	32.0	41.0
23	37.24511119770244	39.0	35.0	41.0	32.0	41.0
24	37.083475356238054	39.0	35.0	40.0	31.0	41.0
25	37.13727612532652	39.0	35.0	41.0	32.0	41.0
26	37.064266512376655	39.0	35.0	40.0	32.0	41.0
27	36.99351876998325	39.0	35.0	40.0	31.0	41.0
28	36.888766873829866	39.0	35.0	40.0	31.0	41.0
29	36.69364511460888	39.0	35.0	40.0	31.0	41.0
30	36.71383532690196	39.0	35.0	40.0	31.0	41.0
31	36.31846061603173	39.0	35.0	40.0	30.0	41.0
32	36.41619205432688	39.0	35.0	40.0	30.0	41.0
33	36.42256438918343	39.0	35.0	40.0	30.0	41.0
34	36.31485789181594	38.0	35.0	40.0	30.0	41.0
35	36.28032238215379	38.0	35.0	40.0	30.0	41.0
36	36.14317614382558	38.0	35.0	40.0	30.0	41.0
37	36.01965360843137	38.0	35.0	40.0	30.0	41.0
38	35.9529481068642	38.0	35.0	40.0	29.0	41.0
39	35.86775141990105	38.0	35.0	40.0	29.0	41.0
40	35.70044909413659	38.0	35.0	40.0	28.0	41.0
41	35.6681164154862	38.0	34.0	40.0	28.0	41.0
42	35.53148054122207	38.0	34.0	40.0	28.0	41.0
43	35.46732030002585	38.0	34.0	40.0	28.0	41.0
44	35.31479491630161	38.0	34.0	40.0	27.0	41.0
45	35.14816826532634	37.0	34.0	40.0	27.0	41.0
46	34.88358582578611	37.0	34.0	40.0	26.0	41.0
47	34.85935468465667	37.0	34.0	40.0	26.0	41.0
48	34.792834173662854	37.0	33.0	40.0	26.0	41.0
49	34.634661985486765	37.0	33.0	40.0	26.0	41.0
50	34.48285163624818	37.0	33.0	40.0	25.0	41.0
51	33.30225754098984	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	11.0
11	11.0
12	13.0
13	16.0
14	23.0
15	66.0
16	108.0
17	294.0
18	489.0
19	837.0
20	1430.0
21	1939.0
22	2725.0
23	3749.0
24	4586.0
25	5648.0
26	6870.0
27	8437.0
28	10464.0
29	13085.0
30	16540.0
31	21402.0
32	27958.0
33	37753.0
34	55410.0
35	72350.0
36	79837.0
37	108028.0
38	142613.0
39	139490.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.471370412791376	32.00691156269803	21.00587640268118	25.51584162182941
2	23.44880156284235	31.391850706047354	19.876121915347788	25.28322581576251
3	21.11529635883448	32.02855939574994	21.291627798966413	25.564516446449165
4	20.486197210447113	33.691244173124936	19.495907247563306	26.326651368864646
5	18.86457771637665	36.041542847621564	17.087093824332428	28.00678561166936
6	19.11962854942463	43.825710016124354	16.899348072227667	20.15531336222335
7	84.50513709638271	7.9392443725474	3.9804461027996547	3.5751724282702333
8	85.06207680126371	7.568344832924649	3.7871899931907724	3.5823883726208705
9	78.59921464285668	11.074900190369732	6.31618168960681	4.009703477166784
10	36.28622896060226	34.53800244292516	13.260281736707247	15.915486859765338
11	26.32179700630149	31.049814943827155	24.209755694364084	18.418632355507274
12	26.948009776948602	25.897893075448604	28.04115974657603	19.112937401026763
13	24.62368850211427	27.954830812344778	26.376244586401747	21.045236099139203
14	21.420858802336916	28.807230638637314	27.382672024833344	22.389238534192422
15	20.38045082596323	32.012684318178536	27.09901981235921	20.507845043499024
16	22.646782148016076	29.668814394103393	26.066090178312546	21.61831327956799
17	22.379923406030695	30.034072377233827	26.091936378986645	21.494067837748833
18	22.742032613444486	30.673798643664863	25.30290566399152	21.281263078899133
19	23.10610980568118	31.16002209390961	25.107943967536123	20.625924132873088
20	24.342397871427615	30.530660547545857	25.06845307208991	20.05848850893662
21	25.02030304342293	29.926095609950654	25.555988512216594	19.49761283440982
22	23.217628945645572	28.463751687546985	25.502065728069105	22.816553638738338
23	22.541035763532193	30.57828578026006	24.3029069759814	22.577771480226343
24	21.446705003011015	31.027773513810665	26.06281020360771	21.462711279570613
25	22.543134947343287	30.613053512131316	23.198473893369336	23.645337647156065
26	23.219203333503895	30.369941787008937	24.42505323398946	21.98580164549771
27	22.478322647175744	28.720245709465086	25.753705387424052	23.047726255935114
28	21.928205289680804	30.582877744846833	27.104005373910557	20.384911591561806
29	22.624347121035	31.128140739778615	24.936466889967345	21.31104524921904
30	23.10886498443324	30.346850765086902	25.8661429203058	20.678141330174064
31	23.91849394057473	29.60229650708934	24.2373074818847	22.241902070451232
32	23.444340797243772	30.078548834231388	25.180759405983462	21.29635096254138
33	23.047726255935114	29.060051088886002	25.078424195192607	22.813798459986277
34	22.452082849537064	29.53354823727599	26.133657657232146	21.880711255954793
35	24.218546026573044	28.037617373894815	26.623554679146316	21.120281920385832
36	24.25895531493661	30.35800267908334	25.293721734817982	20.08932027116207
37	21.777326453258393	31.361018943821904	24.99380084780786	21.86785375511184
38	22.961266122715664	29.994319083811227	25.445125367193167	21.599289426279945
39	22.614769594896885	29.26616469933784	25.361420412725778	22.7576452930395
40	22.942898264368587	29.448400093938474	26.487501328389758	21.121200313303184
41	21.93187886135022	27.91638950880411	28.43108313948683	21.720648490358844
42	22.537493390850972	28.88595003155336	25.766562888267007	22.809993689328667
43	21.890026384116528	28.26039325584721	26.450896810683794	23.398683549352466
44	22.714612024912064	27.707914316564793	25.458507663988893	24.11896599453425
45	22.610177630310115	28.59941144133896	26.450503213719216	22.339907714631703
46	21.45930010587758	28.804213061908868	27.587211247426858	22.14927558478669
47	22.4095743773624	28.513869701036864	27.70752071960021	21.36903520200052
48	22.02870371463695	28.675900451455718	27.33740837390662	21.95798746000071
49	22.08708726438302	29.30906676847708	26.338196879825666	22.265649087314237
50	21.60296299794936	28.641395117560855	26.837540228889758	22.918101655600033
51	21.976486517335978	28.202796900030307	27.14047869262832	22.68023789000539
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16021.0
1	12146.5
2	8272.0
3	5248.0
4	2224.0
5	1954.0
6	1684.0
7	1731.0
8	1778.0
9	1945.0
10	2112.0
11	2187.0
12	2262.0
13	2308.0
14	2354.0
15	2475.5
16	2597.0
17	2422.0
18	2247.0
19	2265.0
20	2283.0
21	2496.5
22	2710.0
23	2973.5
24	3237.0
25	4103.0
26	6317.0
27	7665.0
28	9695.0
29	11725.0
30	14565.5
31	17406.0
32	18718.5
33	20031.0
34	21817.5
35	23604.0
36	25740.0
37	27876.0
38	28709.0
39	29542.0
40	30033.0
41	30524.0
42	32748.0
43	34972.0
44	38924.5
45	42877.0
46	46439.0
47	50001.0
48	53874.0
49	57747.0
50	56886.0
51	56025.0
52	51725.5
53	47426.0
54	43343.5
55	39261.0
56	37943.5
57	36626.0
58	35268.0
59	33910.0
60	32486.0
61	31062.0
62	28523.5
63	25985.0
64	23506.0
65	21027.0
66	19807.5
67	18588.0
68	15952.0
69	13316.0
70	11389.5
71	9463.0
72	8614.5
73	7766.0
74	6395.5
75	4107.0
76	3189.0
77	2582.0
78	1975.0
79	1747.0
80	1519.0
81	1093.0
82	667.0
83	451.5
84	236.0
85	195.0
86	154.0
87	150.5
88	147.0
89	113.5
90	80.0
91	53.5
92	27.0
93	13.5
94	0.0
95	1.0
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	762201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.718838596370325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16069145492393	17.53001295148748
2	9.02029742821214	4.098613627256119
3	3.2391928271196635	2.2077209706555636
4	1.789421991670212	1.6261435763820429
5	1.120986192688706	1.2733752190227199
6	0.7948198640424466	1.0834430522621612
7	0.6082522842881735	0.96731498308316
8	0.4591941108495858	0.8345885511036419
9	0.3622845782381485	0.7407616373051934
>10	3.411063913356607	17.393733206837013
>50	1.1868673637473766	19.369937456250767
>100	0.8399189316283109	29.36807363245968
>500	0.004088617886925018	0.6076244681247672
>1k	0.0023363530782428673	1.1528808427752735
>5k	0.0	0.0
>10k+	5.840882695607168E-4	1.7457758249944177
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13156	1.7260538886724106	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2845	0.3732611214102317	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2640	0.3463653288305841	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1682	0.22067669814130392	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	1521	0.19955366104216604	TruSeq Adapter, Index 14 (95% over 22bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	911	0.11952227824419018	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.247959527736122E-4	0.0	0.0	0.25295164923688107	0.0
2	5.247959527736122E-4	0.0	0.0	0.34623412984239066	0.0
3	5.247959527736122E-4	0.0	0.0	0.4396478094360936	0.0
4	5.247959527736122E-4	0.0	0.0	0.9031738347233866	0.0
5	5.247959527736122E-4	0.0	0.0	0.9833364165095558	0.0
6	5.247959527736122E-4	0.0	0.0	1.496718057310342	0.0
7	5.247959527736122E-4	0.0	0.0	1.8962189763592543	0.0
8	5.247959527736122E-4	0.0	0.0	2.3054286205344785	0.0
9	5.247959527736122E-4	0.0	0.0	2.830355772296284	0.0
10	5.247959527736122E-4	0.0	0.0	3.668979704828516	0.0
11	5.247959527736122E-4	0.0	0.0	4.34649127985925	0.0
12	5.247959527736122E-4	0.0	0.0	4.675144745283724	0.0
13	5.247959527736122E-4	0.0	0.0	4.813559677827764	0.0
14	5.247959527736122E-4	0.0	0.0	4.967062494014046	0.0
15	5.247959527736122E-4	0.0	0.0	5.094981507502614	0.0
16	5.247959527736122E-4	0.0	0.0	5.367219408003925	0.0
17	5.247959527736122E-4	0.0	0.0	5.678948203951451	0.0
18	5.247959527736122E-4	0.0	0.0	6.101671343910596	0.0
19	5.247959527736122E-4	0.0	0.0	6.269999645762732	0.0
20	5.247959527736122E-4	0.0	0.0	6.479393230919404	0.0
21	5.247959527736122E-4	0.0	0.0	6.7057114855530235	0.0
22	5.247959527736122E-4	0.0	0.0	6.934522520962318	0.0
23	5.247959527736122E-4	0.0	0.0	7.177503047096501	0.0
24	5.247959527736122E-4	0.0	0.0	7.347012139842377	0.0
25	5.247959527736122E-4	0.0	0.0	7.508124497343877	0.0
26	6.559949409670153E-4	0.0	0.0	7.660446522636417	0.0
27	6.559949409670153E-4	0.0	0.0	7.836777962768351	0.0
28	6.559949409670153E-4	0.0	0.0	8.012715805935704	0.0
29	6.559949409670153E-4	0.0	0.0	8.188653649103058	0.0
30	6.559949409670153E-4	0.0	0.0	8.405919173551334	0.0
31	6.559949409670153E-4	0.0	0.0	8.573329082486115	0.0
32	6.559949409670153E-4	0.0	0.0	8.766191595130419	0.0
33	6.559949409670153E-4	0.0	0.0	8.95249415836505	0.0
34	6.559949409670153E-4	0.0	0.0	9.12174085313454	0.0
35	6.559949409670153E-4	0.0	0.0	9.304763441664338	0.0
36	6.559949409670153E-4	0.0	0.0	9.475059728339374	0.0
37	6.559949409670153E-4	0.0	0.0	9.640370453463063	0.0
38	6.559949409670153E-4	0.0	0.0	9.823261843004667	0.0
39	6.559949409670153E-4	0.0	0.0	10.010613998144846	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAT	55	1.8189894E-12	45.000004	20
ATCACGC	30	2.1645355E-6	45.000004	42
CGTAGTG	30	2.1645355E-6	45.000004	28
GAAACGT	30	2.1645355E-6	45.000004	35
GCGATTG	55	1.8189894E-12	45.000004	1
AATTCGC	30	2.1645355E-6	45.000004	39
TTAACCG	30	2.1645355E-6	45.000004	1
CACGTTA	30	2.1645355E-6	45.000004	38
CACGTCG	30	2.1645355E-6	45.000004	36
TCGCCTA	20	7.03179E-4	45.0	14
CTTAACG	20	7.03179E-4	45.0	1
CTTCGAA	25	3.8895654E-5	45.0	40
TCGTTTA	35	1.2112105E-7	45.0	38
TCACCGA	25	3.8895654E-5	45.0	38
CGAGTAT	20	7.03179E-4	45.0	34
CATGCGT	35	1.2112105E-7	45.0	34
CGTGATA	50	2.1827873E-11	45.0	14
CGCATAG	20	7.03179E-4	45.0	1
GGTCCAA	45	3.8380676E-10	45.0	10
CACGCGA	20	7.03179E-4	45.0	11
>>END_MODULE