##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626290
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.640457296140767	31.0	31.0	34.0	30.0	34.0
2	31.84412332944802	33.0	31.0	34.0	30.0	34.0
3	32.02267799262322	33.0	31.0	34.0	30.0	34.0
4	35.745614651359595	37.0	35.0	37.0	35.0	37.0
5	35.50477574286672	37.0	35.0	37.0	33.0	37.0
6	32.85826693704195	37.0	35.0	37.0	28.0	37.0
7	34.33217040029379	37.0	35.0	37.0	29.0	37.0
8	35.37819221127592	37.0	35.0	37.0	32.0	37.0
9	37.17875584792987	39.0	37.0	39.0	34.0	39.0
10	36.66944067444794	38.0	37.0	39.0	32.0	39.0
11	36.733739960721074	39.0	37.0	39.0	32.0	39.0
12	36.771318398824825	39.0	37.0	39.0	32.0	39.0
13	36.77192035638442	39.0	37.0	39.0	33.0	39.0
14	37.93106388414313	40.0	37.0	41.0	33.0	41.0
15	37.96852416612113	40.0	37.0	41.0	33.0	41.0
16	37.88867936578901	40.0	37.0	41.0	33.0	41.0
17	37.873566558623004	40.0	37.0	41.0	33.0	41.0
18	37.772669210749015	39.0	37.0	41.0	33.0	41.0
19	37.751460186175734	39.0	36.0	41.0	33.0	41.0
20	37.646272493573264	39.0	36.0	41.0	33.0	41.0
21	37.613503329128676	39.0	36.0	41.0	32.0	41.0
22	37.532195947564226	39.0	36.0	41.0	32.0	41.0
23	37.4177521595427	39.0	36.0	41.0	32.0	41.0
24	37.2680483482093	39.0	36.0	41.0	32.0	41.0
25	37.30336904628846	39.0	36.0	41.0	32.0	41.0
26	37.22839100097399	39.0	36.0	41.0	32.0	41.0
27	37.12566861996839	39.0	35.0	41.0	32.0	41.0
28	37.06932571173099	39.0	35.0	41.0	31.0	41.0
29	36.88520493701001	39.0	35.0	40.0	31.0	41.0
30	36.877971866068435	39.0	35.0	40.0	31.0	41.0
31	36.54090597007776	39.0	35.0	40.0	30.0	41.0
32	36.6182040268885	39.0	35.0	40.0	30.0	41.0
33	36.6040029379361	39.0	35.0	40.0	30.0	41.0
34	36.56765076881317	39.0	35.0	40.0	30.0	41.0
35	36.53229813664596	39.0	35.0	40.0	30.0	41.0
36	36.443537338932444	39.0	35.0	40.0	30.0	41.0
37	36.352897220137635	39.0	35.0	40.0	30.0	41.0
38	36.3109501987897	39.0	35.0	40.0	30.0	41.0
39	36.24196458509636	38.0	35.0	40.0	30.0	41.0
40	36.11304826837408	38.0	35.0	40.0	30.0	41.0
41	36.07550655447157	38.0	35.0	40.0	30.0	41.0
42	35.97215347522713	38.0	35.0	40.0	29.0	41.0
43	35.91242874706606	38.0	35.0	40.0	29.0	41.0
44	35.72831116575389	38.0	35.0	40.0	28.0	41.0
45	35.57833751137652	38.0	34.0	40.0	28.0	41.0
46	35.33322262849479	38.0	34.0	40.0	27.0	41.0
47	35.30482683740759	38.0	34.0	40.0	27.0	41.0
48	35.233348768142555	38.0	34.0	40.0	27.0	41.0
49	35.10213160037682	37.0	34.0	40.0	27.0	41.0
50	34.98588353638091	37.0	34.0	40.0	26.0	41.0
51	33.88976512478245	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	5.0
12	8.0
13	15.0
14	27.0
15	38.0
16	71.0
17	145.0
18	270.0
19	535.0
20	955.0
21	1546.0
22	2144.0
23	2758.0
24	3392.0
25	4090.0
26	4936.0
27	6087.0
28	7744.0
29	9941.0
30	12671.0
31	16769.0
32	21837.0
33	28958.0
34	41666.0
35	56810.0
36	65381.0
37	89431.0
38	122350.0
39	125668.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.942534608567918	32.12728927493653	19.57926839004295	27.3509077264526
2	22.113078605757718	31.28614539590286	20.31981989174344	26.280956106595987
3	20.69408740359897	32.101262993182075	20.376023886697855	26.8286257165211
4	20.344249469095786	33.46213415510387	19.32443436746555	26.869182008334796
5	18.548915039358764	35.546472081623534	17.7443356911335	28.16027718788421
6	18.972999728560254	42.32336457551614	17.62921330374108	21.074422392182534
7	85.06825911319036	7.6608280509029365	3.8028708745150013	3.468041961391687
8	86.49060339459356	7.07771160325089	2.994778776605087	3.4369062255504637
9	78.79193344936051	11.453480017244408	5.819987545705663	3.934598987689409
10	33.11357358412237	40.94253460856792	12.10780948123074	13.836082326078971
11	22.44678982579955	30.009739896852892	29.859490012613964	17.68398026473359
12	24.566574590046145	26.696258921585848	30.22625301377956	18.51091347458845
13	23.804627249357328	26.798128662440725	28.87496207827045	20.5222820099315
14	19.955451947181018	29.776461383704035	28.817321049354135	21.450765619760816
15	19.239489693273086	32.26859761452363	28.064634594197575	20.427278098005715
16	20.73847578597774	30.635648022481597	27.951108911207267	20.67476728033339
17	21.69027128007792	30.003512749684653	28.461894649443547	19.84432132079388
18	21.233773491513517	30.289642178543485	27.352344760414503	21.124239569528495
19	21.150585192163373	31.743281866228106	26.991649235976944	20.114483705631574
20	22.03947053282026	30.678599370898468	27.13136087116192	20.15056922511935
21	23.340145938782353	30.121668875441088	27.047374219610727	19.490810966165835
22	22.089287710166218	28.975235114723212	27.125612735314313	21.80986443979626
23	21.16272014561944	30.04742212074279	26.18323779718661	22.606619936451164
24	18.89444187197624	31.292532213511315	28.29344233502052	21.51958357949193
25	21.181720928004598	31.494515320378742	25.80689456960833	21.516869182008335
26	21.746954286352967	30.827412221175493	25.838509316770185	21.58712417570135
27	19.605933353558257	31.479027287678228	26.958916795733607	21.956122563029908
28	21.009436523016493	30.184100017563747	27.905922176627442	20.900541282792318
29	21.55918184866436	30.486835172204568	26.292771719171633	21.661211259959444
30	22.386913410720275	30.67125453064874	26.97041306742883	19.971418991202157
31	22.45908444969583	31.154896294049085	24.248351402704817	22.137667853550273
32	22.595922016957	30.32796308419422	26.458190295230644	20.617924603618132
33	22.411821999393254	29.7339890466078	25.490108416228903	22.364080537770043
34	21.07490140350317	29.947947436491084	27.22636478308771	21.75078637691804
35	22.58250969997924	28.878953839275734	28.00507751999872	20.5334589407463
36	22.74345750371234	30.997301569560427	26.353446486451965	19.905794440275272
37	20.715802583467724	30.7574765683629	27.242012486228422	21.284708361940954
38	21.14212265883217	30.05077519998723	27.695636206869022	21.11146593431158
39	21.002730364527615	28.973478739880886	27.41908700442287	22.60470389116863
40	22.00546072905523	30.719315333152373	27.13742834788995	20.137795589902442
41	20.09452490060515	29.381596385061233	29.118299829152626	21.405578885180987
42	20.831084641300357	30.62351306902553	26.433122036117453	22.11228025355666
43	20.193041562215587	29.679701096935922	27.358412237142538	22.76884510370595
44	21.515911159367064	28.80790049338166	26.393523766945027	23.282664580306246
45	21.521339954334255	28.994714908429003	27.86839962317776	21.615545514058983
46	20.33083715211803	29.59172268437944	28.712577240575456	21.364862922927077
47	21.025722907918055	30.521323987290234	26.920116878762236	21.532836226029474
48	20.655127816187388	29.32698909450893	28.6333807022306	21.38450238707308
49	20.24253939868112	29.3376870140031	27.967714636989253	22.452058950326524
50	20.14210669178815	28.413514506059496	28.320266968976032	23.124111833176325
51	20.696961471522776	28.146385859585816	28.33112455891041	22.825528109981
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11240.0
1	8474.5
2	5709.0
3	3669.0
4	1629.0
5	1406.0
6	1183.0
7	1248.0
8	1313.0
9	1493.5
10	1674.0
11	1969.0
12	2264.0
13	2446.5
14	2629.0
15	2807.0
16	2985.0
17	2947.0
18	2909.0
19	3036.0
20	3163.0
21	3349.5
22	3536.0
23	4340.0
24	5144.0
25	5396.5
26	6427.5
27	7206.0
28	9201.0
29	11196.0
30	12893.0
31	14590.0
32	16807.0
33	19024.0
34	20117.5
35	21211.0
36	23178.5
37	25146.0
38	25824.5
39	26503.0
40	27519.5
41	28536.0
42	30651.5
43	32767.0
44	35358.0
45	37949.0
46	42239.0
47	46529.0
48	47795.5
49	49062.0
50	48751.0
51	48440.0
52	44311.5
53	40183.0
54	35794.0
55	31405.0
56	30246.0
57	29087.0
58	26524.0
59	23961.0
60	22394.5
61	20828.0
62	18871.0
63	16914.0
64	14932.5
65	12951.0
66	11369.5
67	9788.0
68	8356.0
69	6924.0
70	6385.5
71	5847.0
72	4662.0
73	3477.0
74	2901.0
75	2011.0
76	1697.0
77	1214.5
78	732.0
79	631.0
80	530.0
81	392.0
82	254.0
83	202.5
84	151.0
85	85.0
86	19.0
87	15.0
88	11.0
89	10.0
90	9.0
91	9.0
92	9.0
93	5.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.340396339352967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1473496214481	20.05751369220222
2	8.841212399450054	4.657620774438326
3	3.4946062036649357	2.761479373634881
4	1.9428970961035998	2.0470671823178708
5	1.299515764398337	1.7114880141744715
6	0.9086424695429952	1.436040166711857
7	0.6730535094561557	1.2409947337667313
8	0.554868846163226	1.1692372259439074
9	0.45152677045230355	1.0704054682387552
>10	4.147486254233086	24.940180827277477
>50	1.133269317071752	21.067656778946315
>100	0.39820887210588357	14.644975022240962
>500	0.00429501094721292	0.6530965520595455
>1k	0.002454291969835954	0.9761700505913524
>5k	6.135729924589885E-4	1.5660741374553317
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9690	1.5472065656485017	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2094	0.33434990180267926	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1683	0.26872535087579236	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	1262	0.2015040955467914	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1001	0.15983011065161506	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	716	0.11432403519136501	No Hit
TCAGGCGGGATTATAGCAAGGCCTAGAAGAGATTTACAGACAATCCCAAAG	644	0.10282776349614395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5967044021140367E-4	0.0	0.0	0.24589247792556165	0.0
2	1.5967044021140367E-4	0.0	0.0	0.36021651311692665	0.0
3	1.5967044021140367E-4	0.0	0.0	0.48779319484583816	0.0
4	1.5967044021140367E-4	0.0	0.0	1.0780948123073975	0.0
5	1.5967044021140367E-4	0.0	0.0	1.17166169027128	0.0
6	1.5967044021140367E-4	0.0	0.0	1.739609446103243	0.0
7	1.5967044021140367E-4	0.0	0.0	2.182535247249677	0.0
8	1.5967044021140367E-4	0.0	0.0	2.5941656421146755	0.0
9	1.5967044021140367E-4	0.0	0.0	3.256638298551789	0.0
10	1.5967044021140367E-4	0.0	0.0	4.23733414233023	0.0
11	1.5967044021140367E-4	0.0	0.0	5.110571779846397	0.0
12	1.5967044021140367E-4	0.0	0.0	5.542639991058455	0.0
13	1.5967044021140367E-4	0.0	0.0	5.724504622459244	0.0
14	1.5967044021140367E-4	0.0	0.0	5.90716760606109	0.0
15	1.5967044021140367E-4	0.0	0.0	6.075300579603698	0.0
16	1.5967044021140367E-4	0.0	0.0	6.3856999153746665	0.0
17	1.5967044021140367E-4	0.0	0.0	6.8605598045633815	0.0
18	1.5967044021140367E-4	0.0	0.0	7.339092113876958	0.0
19	1.5967044021140367E-4	0.0	0.0	7.588018330166537	0.0
20	1.5967044021140367E-4	0.0	0.0	7.842692682303725	0.0
21	1.5967044021140367E-4	0.0	0.0	8.12195628223347	0.0
22	1.5967044021140367E-4	0.0	0.0	8.425170448194926	0.0
23	1.5967044021140367E-4	0.0	0.0	8.710341854412492	0.0
24	1.5967044021140367E-4	0.0	0.0	8.922224528573025	0.0
25	1.5967044021140367E-4	0.0	0.0	9.097383001484936	0.0
26	1.5967044021140367E-4	1.5967044021140367E-4	0.0	9.27653323540213	0.0
27	1.5967044021140367E-4	1.5967044021140367E-4	0.0	9.482029091954207	0.0
28	1.5967044021140367E-4	1.5967044021140367E-4	0.0	9.671717574925355	0.0
29	1.5967044021140367E-4	1.5967044021140367E-4	0.0	9.888869373612863	0.0
30	1.5967044021140367E-4	1.5967044021140367E-4	0.0	10.157435054048443	0.0
31	1.5967044021140367E-4	1.5967044021140367E-4	0.0	10.358140797394178	0.0
32	1.5967044021140367E-4	1.5967044021140367E-4	0.0	10.563317313065832	0.0
33	1.5967044021140367E-4	1.5967044021140367E-4	0.0	10.77136789666129	0.0
34	1.5967044021140367E-4	1.5967044021140367E-4	0.0	10.971754299126603	0.0
35	1.5967044021140367E-4	1.5967044021140367E-4	0.0	11.199444346868065	0.0
36	3.1934088042280735E-4	1.5967044021140367E-4	0.0	11.393763272605343	0.0
37	3.1934088042280735E-4	1.5967044021140367E-4	0.0	11.611553753053697	0.0
38	3.1934088042280735E-4	1.5967044021140367E-4	0.0	11.817368950486197	0.0
39	3.1934088042280735E-4	1.5967044021140367E-4	0.0	12.042184930303852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	65	0.0	45.000004	1
AACGTGC	20	7.030824E-4	45.0	24
CCTTAGG	50	2.1827873E-11	45.0	2
CTCCGTA	25	3.8887654E-5	45.0	41
CGAGTCG	20	7.030824E-4	45.0	38
TTAGCCG	40	6.8066583E-9	45.0	1
CGTGCAC	25	3.8887654E-5	45.0	39
CAGCCGA	20	7.030824E-4	45.0	40
CGTGATC	20	7.030824E-4	45.0	20
AGCCACG	25	3.8887654E-5	45.0	1
TCGATAC	25	3.8887654E-5	45.0	24
CGTTCCA	20	7.030824E-4	45.0	44
ACGATGT	20	7.030824E-4	45.0	21
AAGCCCG	20	7.030824E-4	45.0	1
CTAGTCG	20	7.030824E-4	45.0	3
CACTACG	40	6.8066583E-9	45.0	1
TACGAAT	100	0.0	45.0	11
ACGTGCC	20	7.030824E-4	45.0	25
ACTACGT	20	7.030824E-4	45.0	24
TCGTCGA	20	7.030824E-4	45.0	45
>>END_MODULE