##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538306
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.556374998606742	31.0	31.0	34.0	30.0	34.0
2	31.752586818649615	31.0	31.0	34.0	30.0	34.0
3	31.914151059063062	33.0	31.0	34.0	30.0	34.0
4	35.65109621665001	37.0	35.0	37.0	33.0	37.0
5	35.3977923337284	37.0	35.0	37.0	33.0	37.0
6	34.07112311584861	37.0	35.0	37.0	32.0	37.0
7	34.844608828435874	37.0	35.0	37.0	32.0	37.0
8	35.530397209022375	37.0	35.0	37.0	32.0	37.0
9	37.2005160633543	39.0	37.0	39.0	34.0	39.0
10	36.63291139240506	38.0	35.0	39.0	32.0	39.0
11	36.65116866614899	39.0	35.0	39.0	32.0	39.0
12	36.5566685119616	39.0	35.0	39.0	32.0	39.0
13	36.50894658428477	39.0	35.0	39.0	32.0	39.0
14	37.63773021292722	39.0	37.0	41.0	32.0	41.0
15	37.66109053215086	39.0	36.0	41.0	32.0	41.0
16	37.565557508183076	39.0	36.0	41.0	32.0	41.0
17	37.551916567900044	39.0	36.0	41.0	32.0	41.0
18	37.39926918889999	39.0	36.0	41.0	32.0	41.0
19	37.343115254149126	39.0	35.0	41.0	32.0	41.0
20	37.23552960583757	39.0	35.0	40.0	32.0	41.0
21	37.205377610504065	39.0	35.0	40.0	32.0	41.0
22	37.17177033137286	39.0	35.0	40.0	32.0	41.0
23	37.02565455335813	39.0	35.0	40.0	31.0	41.0
24	36.83027125835491	39.0	35.0	40.0	31.0	41.0
25	36.886999216059266	39.0	35.0	40.0	31.0	41.0
26	36.7638982288884	39.0	35.0	40.0	31.0	41.0
27	36.716796766151596	39.0	35.0	40.0	31.0	41.0
28	36.6106563924608	39.0	35.0	40.0	31.0	41.0
29	36.47825214654862	39.0	35.0	40.0	30.0	41.0
30	36.483539102294976	39.0	35.0	40.0	30.0	41.0
31	36.39803940509673	38.0	35.0	40.0	30.0	41.0
32	36.30950240197954	38.0	35.0	40.0	30.0	41.0
33	36.22585295352457	38.0	35.0	40.0	30.0	41.0
34	36.11541762491966	38.0	35.0	40.0	30.0	41.0
35	36.01992732757948	38.0	35.0	40.0	29.0	41.0
36	35.9418787826998	38.0	35.0	40.0	29.0	41.0
37	35.86086723907963	38.0	35.0	40.0	29.0	41.0
38	35.76278547889119	38.0	35.0	40.0	29.0	41.0
39	35.70496334798423	38.0	35.0	40.0	29.0	41.0
40	35.557805411791804	38.0	34.0	40.0	28.0	41.0
41	35.45028478226139	38.0	34.0	40.0	27.0	41.0
42	35.347971228260505	38.0	34.0	40.0	27.0	41.0
43	35.21018156959053	38.0	34.0	40.0	26.0	41.0
44	35.039979119682855	38.0	34.0	40.0	26.0	41.0
45	34.86223077580409	37.0	33.0	40.0	25.0	41.0
46	34.6841833455321	37.0	33.0	40.0	25.0	41.0
47	34.658203698268274	37.0	33.0	40.0	25.0	41.0
48	34.451354805631	37.0	33.0	40.0	24.0	41.0
49	34.26328705234569	37.0	33.0	40.0	24.0	41.0
50	34.13819277511304	36.0	33.0	40.0	24.0	41.0
51	32.8637596460006	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	4.0
11	15.0
12	9.0
13	13.0
14	35.0
15	56.0
16	95.0
17	188.0
18	381.0
19	706.0
20	1105.0
21	1732.0
22	2356.0
23	3127.0
24	3774.0
25	4482.0
26	5288.0
27	6288.0
28	7837.0
29	9851.0
30	12642.0
31	16059.0
32	20476.0
33	27176.0
34	38559.0
35	51971.0
36	56241.0
37	73828.0
38	100170.0
39	93826.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.06218396228168	32.70611882460906	21.022243853867504	25.209453359241767
2	23.06197590218203	33.442874498890966	19.484085260056546	24.011064338870455
3	20.22325591763792	33.651863438267455	21.586792642103187	24.538088001991433
4	21.189806541260918	34.71166957083889	18.655374452448978	25.44314943545121
5	18.067604670949237	37.310377369005735	16.407953840380753	28.21406411966428
6	17.507885849312473	42.5687248516643	18.74770112166686	21.175688177356374
7	81.01005747660253	10.775841250143971	4.4095737368708505	3.8045275363826523
8	82.4662924061779	8.64805519537215	4.104171233462008	4.781481164987944
9	75.95884125385933	12.577233023596243	6.865797520369456	4.598128202174971
10	34.538905380954326	37.70216196735686	12.738479600821837	15.02045305086698
11	25.674430528361192	32.69088585302783	23.439270600736386	18.195413017874593
12	25.92986145426579	28.09071420344562	27.495885239993612	18.483539102294976
13	24.53418687512307	28.419151932172408	26.036120719442103	21.01054047326242
14	20.211924072925065	30.207354181450697	28.825612198266416	20.755109547357822
15	18.92250875895866	32.17222174748191	26.750398472244413	22.154871021315014
16	21.816030287605933	30.60434028229297	27.320148762971247	20.259480667129846
17	21.488707166555827	29.718784483175	26.520975058795553	22.271533291473624
18	21.93715098847124	30.16035489108425	24.89940665718012	23.00308746326439
19	22.57526388336745	30.85828506462867	25.39317785794697	21.173273194056915
20	23.211147562910313	30.81853072416061	25.422157657540506	20.548164055388572
21	23.36886454915977	30.77970522342311	25.314598016741407	20.536832210675712
22	21.63955073879912	30.285190950871808	25.871158783294263	22.20409952703481
23	20.835732835970617	31.497512567201554	24.27708403770346	23.389670559124365
24	19.27881167960231	32.271421830705954	25.292120095261804	23.157646394429936
25	22.78425282274394	30.992409521721846	23.733712795324593	22.48962486020962
26	23.435741009760246	30.387920625071985	23.60757635991425	22.568762005253518
27	20.224742061206825	30.396465950593154	26.149067630678463	23.229724357521558
28	21.701783000746786	31.258614988500966	26.14033653721118	20.89926547354107
29	21.252038803208585	31.017859730339246	24.231756658852028	23.498344807600137
30	21.68302043818943	29.595248799010225	26.095194926305854	22.626535836494487
31	23.922453028574825	30.019357019984916	22.94587093586176	23.1123190155785
32	22.736324692646935	29.299134692907007	25.68279008593625	22.281750528509807
33	22.63080849925507	29.29783431728422	24.289716258039107	23.781640925421602
34	20.87920253536093	29.66918444156298	26.943968672093565	22.507644350982527
35	23.042470267840226	28.748890036521978	25.971473474194973	22.237166221442823
36	22.480893766742334	30.71505797817598	26.026460786244254	20.777587468837428
37	20.71814172608145	31.66507525459497	25.678703191121777	21.938079828201804
38	21.91281538753051	29.685903556713093	27.430866458854258	20.970414596902135
39	20.73820466426159	28.083840789439463	27.572421633791933	23.605532912507012
40	22.726850527395197	28.680527432352605	26.011970886447482	22.580651153804713
41	21.278046315664326	26.774362537292916	28.656191831411874	23.291399315630887
42	23.75247535788195	27.716206024082958	25.624830486749172	22.906488131285922
43	22.338038216181875	27.308445382366166	27.262560699676392	23.09095570177557
44	22.935467930879465	27.070476643396134	27.376436450643315	22.61761897508109
45	22.837010919439873	27.528951934401626	27.285967460886557	22.348069685271945
46	20.82291484768886	27.492355649017476	29.514068206559095	22.17066129673457
47	22.334137089313515	28.195487325053037	26.385921761971815	23.084453823661637
48	21.681720062566644	28.6535910801663	27.36398999825378	22.300698859013274
49	22.16545979424342	27.680167042537146	27.00452902252622	23.14984414069321
50	20.96707077387211	27.736640498155325	27.14385498211055	24.15243374586202
51	21.53254840183836	27.034623429796433	27.703388035801197	23.729440132564005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13244.0
1	10178.0
2	7112.0
3	4356.5
4	1601.0
5	1355.0
6	1109.0
7	1109.5
8	1110.0
9	1199.5
10	1289.0
11	1469.5
12	1650.0
13	1809.0
14	1968.0
15	1988.5
16	2009.0
17	1969.0
18	1929.0
19	2128.5
20	2328.0
21	2240.0
22	2152.0
23	3063.5
24	3975.0
25	4016.0
26	4513.5
27	4970.0
28	6422.0
29	7874.0
30	8907.0
31	9940.0
32	11524.0
33	13108.0
34	13905.0
35	14702.0
36	15720.5
37	16739.0
38	18495.5
39	20252.0
40	20273.0
41	20294.0
42	23622.5
43	26951.0
44	31162.0
45	35373.0
46	37182.0
47	38991.0
48	40097.5
49	41204.0
50	39779.0
51	38354.0
52	35862.5
53	33371.0
54	31005.5
55	28640.0
56	26822.5
57	25005.0
58	24126.5
59	23248.0
60	22615.5
61	21983.0
62	20058.0
63	18133.0
64	16251.0
65	14369.0
66	13057.5
67	11746.0
68	9931.5
69	8117.0
70	7584.0
71	7051.0
72	6070.5
73	5090.0
74	4174.5
75	2542.5
76	1826.0
77	1478.0
78	1130.0
79	729.0
80	328.0
81	367.5
82	407.0
83	260.0
84	113.0
85	81.0
86	49.0
87	41.0
88	33.0
89	30.0
90	27.0
91	25.0
92	23.0
93	37.0
94	51.0
95	36.5
96	22.0
97	11.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.28642733438732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39609842811079	19.823572462018994
2	8.924964928454925	4.513609542506615
3	3.2348322124367854	2.4539204903615444
4	1.6815380031692437	1.7008035410859939
5	0.9996567156064473	1.263887344925736
6	0.6857980022023672	1.0404828809254891
7	0.4979186628092978	0.8813408859893828
8	0.4056136801721623	0.820521667960544
9	0.30308054412018093	0.6897441739825372
>10	3.090426911609125	18.652625216733636
>50	1.1835192285213256	21.104203001621443
>100	0.5899161263657698	22.36188170996047
>500	7.373951579572123E-4	0.10068888102343619
>1k	0.0051617661057004865	2.4480450351049883
>5k	0.0	0.0
>10k+	7.373951579572123E-4	2.144673165799191
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11502	2.136702916185218	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2940	0.5461577615705565	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2235	0.41519135956129044	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	1943	0.3609471192964597	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT	1715	0.3185920275828247	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	1531	0.28441072549813673	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1480	0.27493656024640256	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1285	0.23871181075447792	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	540	0.10031469090071446	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4278235798969359	0.0
2	0.0	0.0	0.0	0.5589757498523145	0.0
3	0.0	0.0	0.0	0.7471586792642103	0.0
4	0.0	0.0	0.0	1.6356867655199832	0.0
5	0.0	0.0	0.0	1.7675820072598114	0.0
6	0.0	0.0	0.0	2.4902193176371803	0.0
7	0.0	0.0	0.0	3.059969608364016	0.0
8	0.0	0.0	0.0	3.522531794183977	0.0
9	0.0	0.0	0.0	4.483137843531375	0.0
10	0.0	0.0	0.0	5.527153700683255	0.0
11	0.0	0.0	0.0	6.6319156762139	0.0
12	3.715358922248684E-4	0.0	0.0	7.087790215973814	0.0
13	3.715358922248684E-4	0.0	0.0	7.288233829829131	0.0
14	3.715358922248684E-4	0.0	0.0	7.5148707240863	0.0
15	3.715358922248684E-4	0.0	0.0	7.703796725282646	0.0
16	3.715358922248684E-4	0.0	0.0	8.07644722518419	0.0
17	3.715358922248684E-4	0.0	0.0	8.533622140566889	0.0
18	3.715358922248684E-4	0.0	0.0	9.178236913577036	0.0
19	3.715358922248684E-4	0.0	0.0	9.419735243523201	0.0
20	3.715358922248684E-4	0.0	0.0	9.703588665183	0.0
21	3.715358922248684E-4	0.0	0.0	9.987813622735024	0.0
22	3.715358922248684E-4	0.0	0.0	10.28207004937712	0.0
23	3.715358922248684E-4	0.0	0.0	10.568709990228607	0.0
24	3.715358922248684E-4	0.0	0.0	10.781042752635118	0.0
25	3.715358922248684E-4	0.0	0.0	10.966253394909215	0.0
26	3.715358922248684E-4	0.0	0.0	11.128057275973145	0.0
27	3.715358922248684E-4	0.0	0.0	11.319769796361177	0.0
28	5.573038383373026E-4	0.0	0.0	11.51222538853366	0.0
29	5.573038383373026E-4	0.0	0.0	11.705981356328929	0.0
30	5.573038383373026E-4	0.0	0.0	11.97553064613807	0.0
31	5.573038383373026E-4	0.0	0.0	12.16631432679554	0.0
32	5.573038383373026E-4	0.0	0.0	12.389978933914911	0.0
33	5.573038383373026E-4	0.0	0.0	12.589493708039665	0.0
34	5.573038383373026E-4	0.0	0.0	12.774704350313762	0.0
35	5.573038383373026E-4	0.0	0.0	12.987965952450837	0.0
36	7.430717844497368E-4	0.0	0.0	13.184694207383904	0.0
37	7.430717844497368E-4	0.0	0.0	13.399070417197654	0.0
38	7.430717844497368E-4	0.0	0.0	13.610660107819715	0.0
39	7.430717844497368E-4	0.0	0.0	13.842684272514147	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTC	30	2.1633423E-6	45.000004	13
TCGTTGC	30	2.1633423E-6	45.000004	23
TGCCCGA	20	7.029939E-4	45.000004	20
CGAACCC	20	7.029939E-4	45.000004	33
CTCGTAT	20	7.029939E-4	45.000004	42
CACGGTT	20	7.029939E-4	45.000004	26
CGACGTA	20	7.029939E-4	45.000004	30
TTTACGA	20	7.029939E-4	45.000004	17
CCCTATA	20	7.029939E-4	45.000004	28
CGTGCGA	20	7.029939E-4	45.000004	43
TAGAATC	20	7.029939E-4	45.000004	28
CATTGCG	30	2.1633423E-6	45.000004	1
CTATACG	30	2.1633423E-6	45.000004	1
AACCACG	20	7.029939E-4	45.000004	1
CCTACGT	30	2.1633423E-6	45.000004	29
ACGGGTA	175	0.0	45.000004	5
TCGATAG	20	7.029939E-4	45.000004	35
ATTCCGC	20	7.029939E-4	45.000004	23
GTTACCA	20	7.029939E-4	45.000004	13
TTCCCGC	30	2.1633423E-6	45.000004	45
>>END_MODULE