SRR3553408_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269635
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16796	6.229161644445268	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4466	1.6563131640921986	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3009	1.1159530476384742	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	1873	0.6946427578022141	TruSeq Adapter, Index 15 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1585	0.5878316984071059	No Hit
AAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	597	0.22141042520444307	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	537	0.1991581211637955	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGT	450	0.16689228030485656	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	423	0.15687874348656516	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	406	0.1505739240083817	No Hit
TAAAGCGGGAGTGATCTTTTTAATAGAAATTAATGAGATAATTTGCCTTGC	405	0.15020305227437092	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402	0.14909043707233852	No Hit
GCACAAGGGCGATGAATTGCGGGGATTTTGAGCGGTGAGACATCTCAGGAC	388	0.14389823279618744	No Hit
AGTGCCGGGAGAAACCTTGATCTTTCAACTGGATTTATGTTTGTGTCTGCT	374	0.13870602852003633	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	373	0.13833515678602554	No Hit
AATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	349	0.12943423516976654	No Hit
TTAAAAGGGACCTAGCTGCAGTCCTTGTCCTCCTTTTTTATCTCTTTTTTT	338	0.1253546460956478	No Hit
TTACAAGGGTAGAATTTAGAATCTGGGAAAGCTACGGGCCATTGTACATTA	319	0.11830808314944276	No Hit
TGATACGGGATGAAATTTTAATTATTGAGCTTAGAATATGTGGGGTTTTTT	316	0.1171954679474104	No Hit
ACCAAAGGGTCTTCTATTTTTTATGTACTTAGAGTTCTTTGAGGTATAGAG	306	0.11348675060730246	No Hit
TAGCACGGGATTTCTTAATGACTGCTCCTTTTCTCTTATTTCATCTTTTTT	297	0.11014890500120533	No Hit
TTGGCAGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG	287	0.1064401876610974	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	271	0.10050623991692473	No Hit
TCAGCTGGGATGATCTGTGGGGTTGTTTTTTGTTTTGTTGTTTTTTTTTTA	270	0.10013536818291394	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGCCA	60	0.0	45.000004	8
ATTACGG	30	2.1593005E-6	45.000004	2
GGATCGT	60	0.0	45.000004	8
GGATCGC	30	2.1593005E-6	45.000004	8
GGATCCC	30	2.1593005E-6	45.000004	8
GGAGTAC	30	2.1593005E-6	45.000004	43
CGGGACG	30	2.1593005E-6	45.000004	6
TTAACGG	60	0.0	45.000004	2
ATAACGG	30	2.1593005E-6	45.000004	2
CCTGGCG	30	2.1593005E-6	45.000004	1
CAGTATA	30	2.1593005E-6	45.000004	29
TCAAGTA	30	2.1593005E-6	45.000004	18
AACACGT	30	2.1593005E-6	45.000004	41
CGGACAG	20	7.0236716E-4	45.0	1
CCTTCCG	25	3.8828377E-5	45.0	1
GTCATCG	20	7.0236716E-4	45.0	42
CGGGTCC	20	7.0236716E-4	45.0	35
CGGGTAC	40	6.7811925E-9	45.0	6
AGACTAG	25	3.8828377E-5	45.0	1
AGGTACC	20	7.0236716E-4	45.0	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content