##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269635
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.405377640143158	31.0	31.0	34.0	30.0	34.0
2	31.609479481521316	31.0	31.0	34.0	30.0	34.0
3	31.802269735012146	31.0	31.0	34.0	30.0	34.0
4	35.57180262206316	37.0	35.0	37.0	33.0	37.0
5	35.30038014352736	37.0	35.0	37.0	33.0	37.0
6	33.87662210024663	37.0	35.0	37.0	31.0	37.0
7	34.71660207317299	37.0	35.0	37.0	32.0	37.0
8	35.39652493185232	37.0	35.0	37.0	32.0	37.0
9	36.98727539080609	39.0	37.0	39.0	33.0	39.0
10	36.453520499935095	38.0	35.0	39.0	32.0	39.0
11	36.58338865503366	38.0	35.0	39.0	32.0	39.0
12	36.60564095907431	38.0	35.0	39.0	32.0	39.0
13	36.56566098614794	39.0	35.0	39.0	32.0	39.0
14	37.66576297587479	39.0	37.0	41.0	32.0	41.0
15	37.67756040573368	39.0	37.0	41.0	32.0	41.0
16	37.55946001075528	39.0	36.0	41.0	32.0	41.0
17	37.45114692083743	39.0	36.0	40.0	32.0	41.0
18	37.176894690971125	38.0	36.0	40.0	32.0	41.0
19	36.921330687781634	38.0	35.0	40.0	32.0	41.0
20	36.796688115415286	38.0	35.0	40.0	31.0	41.0
21	36.71621636656962	38.0	35.0	40.0	31.0	41.0
22	36.66471340886754	38.0	35.0	40.0	31.0	41.0
23	36.40877482522669	38.0	35.0	40.0	31.0	41.0
24	36.23540341572867	38.0	35.0	40.0	30.0	41.0
25	36.28946538839542	38.0	35.0	40.0	31.0	41.0
26	36.2240695755373	38.0	35.0	40.0	30.0	41.0
27	36.15930795334434	38.0	35.0	40.0	30.0	41.0
28	35.99362842360969	38.0	35.0	40.0	30.0	41.0
29	35.84606226936414	38.0	35.0	40.0	30.0	41.0
30	35.75127116286832	38.0	35.0	40.0	29.0	41.0
31	35.64938528010088	38.0	34.0	40.0	29.0	41.0
32	35.43749513230849	38.0	34.0	40.0	28.0	41.0
33	35.341880690563166	38.0	34.0	40.0	27.0	41.0
34	35.234491071263	38.0	34.0	40.0	27.0	41.0
35	35.09124557271868	38.0	34.0	40.0	26.0	41.0
36	35.01515752776902	37.0	34.0	40.0	26.0	41.0
37	34.95223913809409	37.0	34.0	40.0	25.0	41.0
38	34.79912845142508	37.0	33.0	40.0	25.0	41.0
39	34.70544996013129	37.0	33.0	40.0	25.0	41.0
40	34.51653531626087	37.0	33.0	40.0	24.0	41.0
41	34.410881376675874	37.0	33.0	40.0	24.0	41.0
42	34.2157843009995	37.0	33.0	40.0	23.0	41.0
43	34.12864427837632	37.0	33.0	40.0	22.0	41.0
44	33.917703562223004	36.0	33.0	40.0	21.0	41.0
45	33.61180484729356	36.0	33.0	40.0	21.0	41.0
46	33.38187920707623	35.0	33.0	40.0	20.0	41.0
47	33.33646225452927	35.0	33.0	40.0	20.0	41.0
48	33.17458786878558	35.0	32.0	39.0	20.0	41.0
49	33.04861757561147	35.0	32.0	39.0	19.0	41.0
50	32.87982272331114	35.0	32.0	39.0	18.0	41.0
51	31.700487696330224	35.0	30.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	1.0
13	4.0
14	9.0
15	11.0
16	59.0
17	100.0
18	249.0
19	538.0
20	899.0
21	1288.0
22	1782.0
23	2360.0
24	2695.0
25	3055.0
26	3404.0
27	3940.0
28	4743.0
29	5717.0
30	7217.0
31	8998.0
32	11416.0
33	14958.0
34	21114.0
35	34193.0
36	29260.0
37	34031.0
38	42458.0
39	35125.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.628516327628088	36.62395460529976	19.906911194763293	24.84061787230886
2	18.875887774213286	40.53183006657148	17.493648821555066	23.09863333766017
3	17.713575759823467	42.080590427800544	17.62308305672483	22.58275075565116
4	17.15393031320118	41.800953140356405	16.855749439056503	24.189367107385912
5	14.595657091994735	44.63218795779479	15.551764422274555	25.220390527935916
6	15.031802251191426	48.83119773026499	16.982587572088192	19.154412446455392
7	72.43755447178593	18.846959778960446	5.057948708439186	3.657537040814434
8	73.76490440781056	17.752888163628608	3.756930665529327	4.725276763031506
9	66.29109722402508	22.136962931370185	7.654421718248744	3.9175181263559997
10	23.801435273610622	51.917221428968794	11.59382127691138	12.687522020509206
11	16.987408904630332	37.76883564819107	30.834275965657277	14.409479481521315
12	19.831253361025087	33.32690488994381	30.741558032154582	16.10028371687652
13	20.83779924713038	35.139355054054555	27.018376694420237	17.004469004394828
14	15.405270087340291	39.951415802844586	27.425593858364085	17.217720251451034
15	14.28115786155358	41.56137000018544	27.54538542844957	16.61208670981141
16	15.188680994677991	40.43874126133476	25.323492869990915	19.04908487399633
17	14.414300814063456	39.47187865076863	26.42980325254511	19.684017282622804
18	15.115990134811874	39.57127227548353	26.407180076770448	18.905557512934152
19	16.63025942477794	42.96734474382035	24.16526044467521	16.2371353867265
20	15.898529493574648	41.70230125910954	24.447493834257422	17.951675413058393
21	16.89617445806368	42.49893374376472	23.94459176293879	16.660300035232815
22	15.053683683498061	40.012980510690376	24.517217720251452	20.416118085560107
23	15.576612828453278	41.2405659502661	22.494112411222577	20.68870881005804
24	12.63782520815176	43.153893226027776	24.878446789177964	19.3298347766425
25	14.19659910619912	43.564819107311735	22.80008159178148	19.43850019470766
26	15.0343983533295	42.54603445398409	24.51684684851744	17.90272034416897
27	14.49589259554583	42.141413392178315	22.937675005099486	20.425019007176367
28	15.85254139855731	42.08021955606653	25.086505831958018	16.98073321341814
29	17.02227084762735	42.11582324253157	22.51858994566729	18.34331596417379
30	17.63606356741521	43.66421273202663	21.66261798357038	17.03710571698778
31	20.02002707363658	41.17492165334619	19.527509410870252	19.277541862146975
32	18.21462347247205	42.058338123759896	20.60377918296957	19.123259220798484
33	17.900865985498914	41.11966176497858	21.191981753110685	19.787490496411817
34	15.116731878279897	41.951897936098796	23.180225119142545	19.75114506647876
35	17.36384371465129	41.556919539377304	22.164036567952973	18.91520017801843
36	18.700094572292173	40.34120199528993	24.36664379624307	16.59205963617483
37	19.741873273128487	41.26615609991284	19.932872216144045	19.05909841081462
38	19.323529957164315	40.85077975782076	21.86325959167022	17.962430693344707
39	19.890221966732806	36.18261724182691	22.73777514046767	21.18938565097261
40	19.099152558087784	37.15949338921134	22.039423665325348	21.701930387375526
41	16.60207317299312	34.73658835091884	25.6253824614757	23.035956014612346
42	18.045135090029113	38.50241993806442	23.21063660133143	20.241808370575036
43	16.5497802584976	36.62951768130992	25.946557383129043	20.874144677063438
44	19.245646893022048	36.758581044745675	23.007769762827525	20.98800229940475
45	20.356036864650363	37.56782316835722	24.392604817623827	17.68353514936859
46	17.19991840821852	38.123018154171376	26.756541250208617	17.920522187401485
47	18.440855230218627	36.02907634394645	24.683368257088286	20.84670016874664
48	17.59267157453595	35.42418454577484	26.78176052812135	20.201383351567863
49	18.658927809816976	34.207354386485434	25.34945389137167	21.784263912325923
50	18.05700298551746	33.0758247260185	25.64392604817624	23.223246240287796
51	17.96947725629091	33.48860496597252	26.77211786303707	21.7697999146995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18787.0
1	14367.0
2	9947.0
3	6152.5
4	2358.0
5	1843.0
6	1328.0
7	1469.0
8	1610.0
9	1830.5
10	2051.0
11	2325.5
12	2600.0
13	2848.5
14	3097.0
15	3017.5
16	2938.0
17	2891.0
18	2844.0
19	2691.0
20	2538.0
21	2732.5
22	2927.0
23	2636.0
24	2345.0
25	2692.5
26	2993.5
27	2947.0
28	3641.0
29	4335.0
30	4790.5
31	5246.0
32	5903.5
33	6561.0
34	6647.5
35	6734.0
36	7905.0
37	9076.0
38	8315.5
39	7555.0
40	8185.0
41	8815.0
42	10608.0
43	12401.0
44	14609.0
45	16817.0
46	19428.0
47	22039.0
48	24004.0
49	25969.0
50	25162.0
51	24355.0
52	20847.0
53	17339.0
54	14689.0
55	12039.0
56	9909.0
57	7779.0
58	7159.5
59	6540.0
60	5539.5
61	4539.0
62	3887.0
63	3235.0
64	2869.0
65	2503.0
66	2079.5
67	1656.0
68	1348.0
69	1040.0
70	905.0
71	770.0
72	575.5
73	381.0
74	316.5
75	196.5
76	141.0
77	94.0
78	47.0
79	48.5
80	50.0
81	35.0
82	20.0
83	25.5
84	31.0
85	20.5
86	10.0
87	5.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.55597381645558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69492889723638	27.19379902460734
2	9.029246042393346	6.2402877964655925
3	3.540649315803596	3.6705175515048123
4	1.7311510598336461	2.3928644278376323
5	1.0786155084518378	1.8636304634042318
6	0.8285484303729541	1.7178778719379901
7	0.6686342903139254	1.6173716320210654
8	0.4743761738663805	1.3114024514621618
9	0.3992487255165012	1.2416785654681328
>10	2.929970485645291	20.66052255827322
>50	0.4196404614971827	10.01094071615332
>100	0.19747786423396835	11.374636082111001
>500	0.0021464985242822645	0.4205685463682386
>1k	0.004292997048564529	4.054740667939993
>5k	0.0	0.0
>10k+	0.0010732492621411322	6.229161644445268
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16796	6.229161644445268	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4466	1.6563131640921986	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3009	1.1159530476384742	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	1873	0.6946427578022141	TruSeq Adapter, Index 15 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1585	0.5878316984071059	No Hit
AAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	597	0.22141042520444307	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	537	0.1991581211637955	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGT	450	0.16689228030485656	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	423	0.15687874348656516	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	406	0.1505739240083817	No Hit
TAAAGCGGGAGTGATCTTTTTAATAGAAATTAATGAGATAATTTGCCTTGC	405	0.15020305227437092	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402	0.14909043707233852	No Hit
GCACAAGGGCGATGAATTGCGGGGATTTTGAGCGGTGAGACATCTCAGGAC	388	0.14389823279618744	No Hit
AGTGCCGGGAGAAACCTTGATCTTTCAACTGGATTTATGTTTGTGTCTGCT	374	0.13870602852003633	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	373	0.13833515678602554	No Hit
AATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	349	0.12943423516976654	No Hit
TTAAAAGGGACCTAGCTGCAGTCCTTGTCCTCCTTTTTTATCTCTTTTTTT	338	0.1253546460956478	No Hit
TTACAAGGGTAGAATTTAGAATCTGGGAAAGCTACGGGCCATTGTACATTA	319	0.11830808314944276	No Hit
TGATACGGGATGAAATTTTAATTATTGAGCTTAGAATATGTGGGGTTTTTT	316	0.1171954679474104	No Hit
ACCAAAGGGTCTTCTATTTTTTATGTACTTAGAGTTCTTTGAGGTATAGAG	306	0.11348675060730246	No Hit
TAGCACGGGATTTCTTAATGACTGCTCCTTTTCTCTTATTTCATCTTTTTT	297	0.11014890500120533	No Hit
TTGGCAGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG	287	0.1064401876610974	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	271	0.10050623991692473	No Hit
TCAGCTGGGATGATCTGTGGGGTTGTTTTTTGTTTTGTTGTTTTTTTTTTA	270	0.10013536818291394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9223580024848406	0.0
2	0.0	0.0	0.0	1.218313646225453	0.0
3	0.0	0.0	0.0	1.545051643888961	0.0
4	0.0	0.0	0.0	3.123852615572904	0.0
5	0.0	0.0	0.0	3.379754112040351	0.0
6	0.0	0.0	0.0	4.842843102712926	0.0
7	0.0	0.0	0.0	5.988095017338254	0.0
8	0.0	0.0	0.0	7.18452723125707	0.0
9	0.0	0.0	0.0	8.767778663749143	0.0
10	0.0	0.0	0.0	11.399855360023736	0.0
11	0.0	0.0	0.0	13.289446844808722	0.0
12	0.0	0.0	0.0	14.331225545645038	0.0
13	0.0	0.0	0.0	14.750681476811245	0.0
14	0.0	0.0	0.0	15.1271162868322	0.0
15	0.0	0.0	0.0	15.459046488771857	0.0
16	0.0	0.0	0.0	16.170378474604558	0.0
17	0.0	0.0	0.0	17.03191351271163	0.0
18	0.0	0.0	0.0	18.474975429747623	0.0
19	0.0	0.0	0.0	18.921504997496616	0.0
20	0.0	0.0	0.0	19.496727056947353	0.0
21	0.0	0.0	0.0	19.959574980992823	0.0
22	0.0	0.0	0.0	20.499564225712536	0.0
23	0.0	0.0	0.0	21.00172455356315	0.0
24	0.0	0.0	0.0	21.331058653364735	0.0
25	0.0	0.0	0.0	21.61996773415914	0.0
26	0.0	0.0	0.0	21.893300202125094	0.0
27	0.0	0.0	0.0	22.221150814990636	0.0
28	0.0	0.0	0.0	22.56902850149276	0.0
29	0.0	0.0	0.0	22.889090807944072	0.0
30	0.0	0.0	0.0	23.367144473083982	0.0
31	0.0	0.0	0.0	23.701299905427707	0.0
32	0.0	0.0	0.0	24.05362805273796	0.0
33	0.0	0.0	0.0	24.355888515956757	0.0
34	0.0	0.0	0.0	24.698573998182727	0.0
35	0.0	0.0	0.0	25.02197415024014	0.0
36	0.0	0.0	0.0	25.352791736977768	0.0
37	0.0	0.0	0.0	25.652456098058487	0.0
38	0.0	0.0	0.0	25.960279637287446	0.0
39	0.0	0.0	0.0	26.287388506684962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGCCA	60	0.0	45.000004	8
ATTACGG	30	2.1593005E-6	45.000004	2
GGATCGT	60	0.0	45.000004	8
GGATCGC	30	2.1593005E-6	45.000004	8
GGATCCC	30	2.1593005E-6	45.000004	8
GGAGTAC	30	2.1593005E-6	45.000004	43
CGGGACG	30	2.1593005E-6	45.000004	6
TTAACGG	60	0.0	45.000004	2
ATAACGG	30	2.1593005E-6	45.000004	2
CCTGGCG	30	2.1593005E-6	45.000004	1
CAGTATA	30	2.1593005E-6	45.000004	29
TCAAGTA	30	2.1593005E-6	45.000004	18
AACACGT	30	2.1593005E-6	45.000004	41
CGGACAG	20	7.0236716E-4	45.0	1
CCTTCCG	25	3.8828377E-5	45.0	1
GTCATCG	20	7.0236716E-4	45.0	42
CGGGTCC	20	7.0236716E-4	45.0	35
CGGGTAC	40	6.7811925E-9	45.0	6
AGACTAG	25	3.8828377E-5	45.0	1
AGGTACC	20	7.0236716E-4	45.0	12
>>END_MODULE