##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	880519
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79645413670801	31.0	31.0	34.0	30.0	34.0
2	31.99084062921981	33.0	31.0	34.0	30.0	34.0
3	32.14743123089905	34.0	31.0	34.0	30.0	34.0
4	35.860433448909106	37.0	35.0	37.0	35.0	37.0
5	35.63500617249599	37.0	35.0	37.0	33.0	37.0
6	32.869963055879545	37.0	35.0	37.0	28.0	37.0
7	34.326107670589735	37.0	35.0	37.0	28.0	37.0
8	35.415899032275284	37.0	35.0	37.0	32.0	37.0
9	37.26384439177349	39.0	37.0	39.0	34.0	39.0
10	36.839716121968976	39.0	37.0	39.0	32.0	39.0
11	36.905696526707544	39.0	37.0	39.0	33.0	39.0
12	36.853450067516995	39.0	37.0	39.0	33.0	39.0
13	36.83952305401701	39.0	37.0	39.0	33.0	39.0
14	38.01441081907375	40.0	37.0	41.0	33.0	41.0
15	38.07055043673106	40.0	37.0	41.0	33.0	41.0
16	37.97946665546115	40.0	37.0	41.0	33.0	41.0
17	37.9432527861409	40.0	37.0	41.0	33.0	41.0
18	37.807072874066314	39.0	37.0	41.0	33.0	41.0
19	37.7813687154962	40.0	36.0	41.0	33.0	41.0
20	37.672878154815514	39.0	36.0	41.0	33.0	41.0
21	37.62977403099763	39.0	36.0	41.0	33.0	41.0
22	37.53835294865869	39.0	36.0	41.0	33.0	41.0
23	37.38212576900669	39.0	36.0	41.0	32.0	41.0
24	37.30250681700225	39.0	35.0	41.0	32.0	41.0
25	37.278237039745875	39.0	35.0	41.0	32.0	41.0
26	37.23300462568099	39.0	35.0	41.0	32.0	41.0
27	37.12071289773418	39.0	35.0	41.0	32.0	41.0
28	37.000122654934195	39.0	35.0	40.0	31.0	41.0
29	36.82075344200409	39.0	35.0	40.0	31.0	41.0
30	36.887709407747025	39.0	35.0	40.0	31.0	41.0
31	36.51038989505053	39.0	35.0	40.0	30.0	41.0
32	36.618683980697746	39.0	35.0	40.0	30.0	41.0
33	36.57688249770874	39.0	35.0	40.0	31.0	41.0
34	36.540673171163824	39.0	35.0	40.0	31.0	41.0
35	36.484303007657985	39.0	35.0	40.0	30.0	41.0
36	36.37463586816412	39.0	35.0	40.0	30.0	41.0
37	36.29107038008266	38.0	35.0	40.0	30.0	41.0
38	36.19116793618309	38.0	35.0	40.0	30.0	41.0
39	36.08462509042962	38.0	35.0	40.0	30.0	41.0
40	35.96917272653969	38.0	35.0	40.0	30.0	41.0
41	35.91378720958889	38.0	35.0	40.0	29.0	41.0
42	35.81990053593392	38.0	35.0	40.0	29.0	41.0
43	35.70645494305063	38.0	35.0	40.0	29.0	41.0
44	35.52248276300682	38.0	34.0	40.0	28.0	41.0
45	35.383895180001794	38.0	34.0	40.0	27.0	41.0
46	35.14356987185966	38.0	34.0	40.0	26.0	41.0
47	35.07732939323285	37.0	34.0	40.0	26.0	41.0
48	35.078131193080445	37.0	34.0	40.0	27.0	41.0
49	34.9216859602121	37.0	34.0	40.0	26.0	41.0
50	34.80742266776753	37.0	34.0	40.0	26.0	41.0
51	33.714584239522374	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	10.0
10	12.0
11	23.0
12	29.0
13	35.0
14	49.0
15	89.0
16	119.0
17	263.0
18	523.0
19	910.0
20	1424.0
21	2287.0
22	3296.0
23	4256.0
24	4972.0
25	6145.0
26	7057.0
27	8424.0
28	10316.0
29	13214.0
30	17035.0
31	22467.0
32	29841.0
33	41405.0
34	61756.0
35	82241.0
36	90351.0
37	124809.0
38	170486.0
39	176648.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.66565287063652	31.654172141657362	21.128561677828646	26.55161330987747
2	22.701611208843875	32.47323453554097	20.186617210985794	24.63853704462936
3	20.771840244219604	32.641090084370695	21.05122092765744	25.535848743752265
4	21.501864241430336	32.872203779816225	18.676144410285296	26.949787568468142
5	18.0850157691089	36.71073537311517	16.579880729433434	28.624368128342486
6	17.009740846023767	44.298873732423715	17.72136660310567	20.97001881844685
7	83.19627401566576	9.504280997911458	4.020810453834613	3.278634532588167
8	84.71049460602214	8.023109098156883	3.453985660729638	3.8124106350913496
9	77.93903368354346	12.412452201485715	5.750017887177903	3.898496227792927
10	35.8197835594689	38.921249853779415	12.525680876846495	12.733285709905182
11	25.382984353546036	32.04632722292193	24.249675475486615	18.321012948045414
12	26.531170820845436	26.855638549537264	27.53489703231844	19.078293597298863
13	24.603330535740852	27.921260075023934	25.328698188227627	22.146711201007587
14	20.683142555697266	30.74027931254181	25.928003825016837	22.64857430674409
15	20.367078961385275	31.9044790629163	26.9132182269775	20.815223748720925
16	22.36272016844611	31.865070486837876	25.614325187758585	20.157884156957433
17	22.709106788155623	30.623302847525153	25.91085484810663	20.756735516212597
18	22.92102725778774	31.173773649404502	24.75256070567472	21.152638387133045
19	22.76657289621235	31.97159856857149	24.988898592761768	20.272929942454393
20	23.739294665986765	30.52211252681657	25.671564157048287	20.067028650148377
21	25.82374713095345	30.29565517609501	25.12097978578543	18.759617907166117
22	23.97131691650038	29.852621011017366	24.840804116663012	21.33525795581924
23	23.178716188974914	30.236031249751566	24.87078643391	21.714466127363522
24	21.39579043723077	30.280436878704492	26.747406927050974	21.57636575701376
25	22.509111103792197	32.30378901534209	24.115663602943265	21.071436277922455
26	23.953145815138573	31.975573496994386	22.511496060845936	21.55978462702111
27	20.741062941288035	31.66019131898346	24.82149732146609	22.77724841826241
28	21.385682762098263	31.946499734815493	27.468799651114857	19.199017851971394
29	22.12695012827662	30.912564067328475	25.52176614019686	21.438719664198047
30	22.55113177569138	30.868499146526084	25.491783822949877	21.08858525483266
31	22.792353146269416	31.673365367470772	23.70181676942803	21.83246471683178
32	23.582114639207106	30.103382209810352	25.54050508847623	20.77399806250632
33	23.16406573850195	30.690081645029803	25.497803000275976	20.648049616192267
34	21.67801035525639	30.409565267756854	26.995215321872667	20.917209055114085
35	22.16056666579597	29.8278628854119	27.212359983146307	20.799210465645828
36	23.63356156993773	32.130709274870846	24.913261383343233	19.322467771848196
37	22.521717305361953	31.81373712549076	24.986513635708032	20.678031933439257
38	22.450282163133334	30.19787193689176	26.547070534537013	20.804775365437884
39	21.905603399813064	29.930529608106127	27.060517717391676	21.10334927468913
40	23.913850808443655	30.10917424836943	25.954579060758487	20.02239588242843
41	21.06950559840276	29.796858443713308	27.35477599006949	21.778859967814437
42	22.90524111347966	29.629797880568166	25.911649833791206	21.553311172160964
43	22.61995482209924	29.368361159725115	26.452012960538045	21.559671057637598
44	23.30545962097354	26.987606173177408	26.34003354839589	23.36690065745316
45	23.025283951851126	29.23400857903123	26.272346195823143	21.4683612732945
46	22.286515112110017	29.194827141719827	27.24904289402046	21.269614852149697
47	21.566371651264767	29.475002810842245	26.943768391142044	22.01485714675095
48	22.75726020676442	27.605537188862478	28.0483442151731	21.588858389200006
49	21.769093000832463	29.059679575341363	27.177494182408328	21.993733241417846
50	21.736725726531738	27.392935302929295	28.289452016367623	22.580886954171348
51	21.593287595156944	26.83303824221851	27.917739424135085	23.65593473848946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20021.0
1	14998.0
2	9975.0
3	6287.0
4	2599.0
5	2196.5
6	1794.0
7	1853.5
8	1913.0
9	1999.5
10	2086.0
11	2275.5
12	2465.0
13	2827.5
14	3190.0
15	3192.5
16	3195.0
17	3324.0
18	3453.0
19	3904.0
20	4355.0
21	4027.0
22	3699.0
23	5262.0
24	6825.0
25	6797.0
26	7751.0
27	8733.0
28	12011.5
29	15290.0
30	17770.5
31	20251.0
32	22547.5
33	24844.0
34	26951.0
35	29058.0
36	32557.5
37	36057.0
38	35558.0
39	35059.0
40	36335.5
41	37612.0
42	39252.5
43	40893.0
44	44875.5
45	48858.0
46	54013.5
47	59169.0
48	60558.0
49	61947.0
50	59546.0
51	57145.0
52	55456.0
53	53767.0
54	49273.0
55	44779.0
56	42196.5
57	39614.0
58	38895.5
59	38177.0
60	36490.5
61	34804.0
62	32914.0
63	31024.0
64	28262.0
65	25500.0
66	22294.0
67	19088.0
68	16922.0
69	14756.0
70	13020.5
71	11285.0
72	10004.0
73	8723.0
74	6780.0
75	3628.5
76	2420.0
77	2293.0
78	2166.0
79	1604.0
80	1042.0
81	798.5
82	555.0
83	459.5
84	364.0
85	245.0
86	126.0
87	121.0
88	116.0
89	101.5
90	87.0
91	51.5
92	16.0
93	14.5
94	13.0
95	8.0
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	880519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.9202769537289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.82805248379961	16.072641357767072
2	8.796394989658278	3.680460387560889
3	3.247964432525368	2.038449463928749
4	1.753605588155911	1.4674365828731326
5	1.1496288121486493	1.202527657206806
6	0.8260493702707112	1.0368708962109978
7	0.6169321405187354	0.9034473868916117
8	0.4859030931493611	0.8132181825086533
9	0.4101023838820262	0.7721509905176792
>10	3.61695483411132	17.002005334218882
>50	1.2971016122232109	19.569090088539742
>100	0.9646952067108437	31.100331649245756
>500	0.0016537632115043036	0.3106827159170124
>1k	0.004410035230678143	2.0278563016647166
>5k	0.0	0.0
>10k+	5.512544038347679E-4	2.0028310049483125
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17367	1.9723594834410159	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3608	0.40975833570882625	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3350	0.38045743476290683	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2785	0.3162907330790136	No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	2284	0.2593924719398446	Illumina Single End Adapter 2 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1900	0.21578182867149942	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	1385	0.15729359616317196	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC	1174	0.13333045624228437	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1098	0.12469918309542441	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	920	0.10448383283041024	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	914	0.10380241652934236	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2713876702263098E-4	0.0	0.0	0.2977789235666692	0.0
2	2.2713876702263098E-4	0.0	0.0	0.4085090724902018	0.0
3	2.2713876702263098E-4	0.0	0.0	0.5267348007254812	0.0
4	2.2713876702263098E-4	0.0	0.0	1.0560816972717226	0.0
5	2.2713876702263098E-4	0.0	0.0	1.1531835201738974	0.0
6	2.2713876702263098E-4	0.0	0.0	1.5859964407355207	0.0
7	2.2713876702263098E-4	0.0	0.0	1.925568897434354	0.0
8	2.2713876702263098E-4	0.0	0.0	2.1963183077253303	0.0
9	2.2713876702263098E-4	0.0	0.0	2.715216820988531	0.0
10	2.2713876702263098E-4	0.0	0.0	3.3082761416846203	0.0
11	2.2713876702263098E-4	0.0	0.0	3.9091717498429905	0.0
12	2.2713876702263098E-4	0.0	0.0	4.165952125962074	0.0
13	2.2713876702263098E-4	0.0	0.0	4.30450677384588	0.0
14	2.2713876702263098E-4	0.0	0.0	4.461800370009051	0.0
15	2.2713876702263098E-4	0.0	0.0	4.586499553104476	0.0
16	2.2713876702263098E-4	0.0	0.0	4.83805573758204	0.0
17	2.2713876702263098E-4	0.0	0.0	5.11777712917041	0.0
18	2.2713876702263098E-4	0.0	0.0	5.57273607951674	0.0
19	2.2713876702263098E-4	0.0	0.0	5.739569503894862	0.0
20	2.2713876702263098E-4	0.0	0.0	5.961143371125439	0.0
21	2.2713876702263098E-4	0.0	0.0	6.170792453087327	0.0
22	2.2713876702263098E-4	0.0	0.0	6.3938427223035506	0.0
23	2.2713876702263098E-4	0.0	0.0	6.614508034466036	0.0
24	2.2713876702263098E-4	0.0	0.0	6.77793437733882	0.0
25	2.2713876702263098E-4	0.0	0.0	6.92989021247696	0.0
26	2.2713876702263098E-4	0.0	0.0	7.06117641981604	0.0
27	2.2713876702263098E-4	0.0	0.0	7.257878592057638	0.0
28	2.2713876702263098E-4	0.0	0.0	7.418692839109662	0.0
29	2.2713876702263098E-4	0.0	0.0	7.57223864561696	0.0
30	2.2713876702263098E-4	0.0	0.0	7.8035794798295095	0.0
31	2.2713876702263098E-4	0.0	0.0	7.962235908594818	0.0
32	2.2713876702263098E-4	0.0	0.0	8.129182902356451	0.0
33	2.2713876702263098E-4	0.0	0.0	8.29045142694252	0.0
34	2.2713876702263098E-4	0.0	0.0	8.450924965844008	0.0
35	2.2713876702263098E-4	0.0	0.0	8.621392610494492	0.0
36	2.2713876702263098E-4	0.0	0.0	8.774370570084235	0.0
37	3.407081505339465E-4	0.0	0.0	8.936206941587859	0.0
38	3.407081505339465E-4	0.0	0.0	9.09872472939255	0.0
39	3.407081505339465E-4	0.0	0.0	9.288839877390494	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.0323853E-4	45.000004	29
AACCGTA	20	7.0323853E-4	45.000004	10
CGAGTAG	35	1.2115015E-7	45.000004	1
TTAGCCA	20	7.0323853E-4	45.000004	29
CACGATT	20	7.0323853E-4	45.000004	41
CGCGATA	20	7.0323853E-4	45.000004	26
CGCGACG	75	0.0	45.000004	1
TAACGCG	20	7.0323853E-4	45.000004	1
CGCGAAA	20	7.0323853E-4	45.000004	14
GTTACGA	20	7.0323853E-4	45.000004	10
ACGATAT	35	1.2115015E-7	45.000004	23
CCACTAG	70	0.0	45.000004	1
CCGTAGA	20	7.0323853E-4	45.000004	16
CGGATTG	20	7.0323853E-4	45.000004	1
CGTAGCG	40	6.8121153E-9	45.000004	1
CGCTAAC	20	7.0323853E-4	45.000004	16
GTACTAG	20	7.0323853E-4	45.000004	1
ACTCGTA	20	7.0323853E-4	45.000004	37
GCGATAT	20	7.0323853E-4	45.000004	18
CCGACTC	20	7.0323853E-4	45.000004	13
>>END_MODULE