##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432679
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38168249441272	31.0	31.0	34.0	28.0	34.0
2	31.587000986874795	31.0	31.0	34.0	30.0	34.0
3	31.79594341301519	31.0	31.0	34.0	30.0	34.0
4	35.57153686682275	37.0	35.0	37.0	33.0	37.0
5	35.30443122961826	37.0	35.0	37.0	33.0	37.0
6	32.74786157867611	37.0	35.0	37.0	27.0	37.0
7	34.15881750674287	37.0	35.0	37.0	28.0	37.0
8	35.12370371568761	37.0	35.0	37.0	32.0	37.0
9	36.848855618137236	39.0	37.0	39.0	32.0	39.0
10	36.43368409375079	38.0	35.0	39.0	32.0	39.0
11	36.564270510008576	38.0	35.0	39.0	32.0	39.0
12	36.61392625942096	38.0	35.0	39.0	32.0	39.0
13	36.61587689719168	39.0	35.0	39.0	32.0	39.0
14	37.69064364112887	39.0	37.0	41.0	32.0	41.0
15	37.74316063409594	39.0	37.0	41.0	32.0	41.0
16	37.67356169354186	39.0	37.0	41.0	32.0	41.0
17	37.590765902666874	39.0	36.0	40.0	32.0	41.0
18	37.23994000171027	38.0	36.0	40.0	32.0	41.0
19	36.993013296231155	39.0	35.0	40.0	32.0	41.0
20	36.83998298969906	38.0	35.0	40.0	31.0	41.0
21	36.72388768579016	38.0	35.0	40.0	31.0	41.0
22	36.618793609119	38.0	35.0	40.0	31.0	41.0
23	36.410916637969486	38.0	35.0	40.0	31.0	41.0
24	36.317741327866614	38.0	35.0	40.0	31.0	41.0
25	36.31369675902921	38.0	35.0	40.0	31.0	41.0
26	36.20433624003014	38.0	35.0	40.0	30.0	41.0
27	36.091853313888585	38.0	35.0	40.0	30.0	41.0
28	35.96177304653103	38.0	35.0	40.0	30.0	41.0
29	35.730915990838476	38.0	34.0	40.0	29.0	41.0
30	35.66726141088428	38.0	34.0	40.0	29.0	41.0
31	35.19000459925256	37.0	34.0	40.0	26.0	41.0
32	35.20793475070433	37.0	34.0	40.0	26.0	41.0
33	35.1217484555525	37.0	34.0	40.0	26.0	41.0
34	35.14446275414337	37.0	34.0	40.0	27.0	41.0
35	35.06827463315761	37.0	34.0	40.0	26.0	41.0
36	34.83588988603561	37.0	33.0	40.0	25.0	41.0
37	34.70521795603669	37.0	33.0	40.0	25.0	41.0
38	34.53496240862163	37.0	33.0	40.0	24.0	41.0
39	34.436635473411	37.0	33.0	40.0	24.0	41.0
40	34.381622403675706	37.0	33.0	40.0	23.0	41.0
41	34.33971142579141	37.0	33.0	40.0	23.0	41.0
42	34.152981771706045	36.0	33.0	40.0	23.0	41.0
43	33.97365945654862	36.0	33.0	40.0	22.0	41.0
44	33.76705363560515	36.0	33.0	40.0	21.0	41.0
45	33.62643437744841	36.0	33.0	40.0	22.0	41.0
46	33.293996242017755	35.0	32.0	40.0	20.0	41.0
47	33.36510207336155	35.0	32.0	39.0	20.0	41.0
48	33.361295556289996	35.0	32.0	39.0	20.0	41.0
49	33.16606075173512	35.0	32.0	39.0	20.0	41.0
50	32.98376625627775	35.0	32.0	39.0	20.0	41.0
51	32.0781040910236	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	4.0
12	3.0
13	5.0
14	15.0
15	35.0
16	82.0
17	185.0
18	435.0
19	781.0
20	1317.0
21	2001.0
22	2758.0
23	3538.0
24	4376.0
25	5120.0
26	5679.0
27	6719.0
28	7781.0
29	9528.0
30	11792.0
31	14823.0
32	18648.0
33	24494.0
34	34619.0
35	56763.0
36	48328.0
37	55366.0
38	64577.0
39	52897.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.633857894651694	38.2611589654224	20.22469313278435	23.880290007141554
2	18.287229100557227	42.13354472946457	18.56457096369364	21.014655206284566
3	17.225934237621885	42.984984249293355	18.230374018614263	21.558707494470497
4	16.282740784738802	42.55834001650184	17.918133304366517	23.24078589439284
5	13.74205820018998	45.545080764261726	16.771555818516727	23.941305217031562
6	15.184004770280046	50.83999916797441	15.640001016920166	18.335995044825378
7	70.3798890170311	20.348803616537893	4.930907208346142	4.3404001580848615
8	72.29216116335667	19.919617083334295	3.8703057000686423	3.917916053240393
9	65.45221746375488	24.303467466643863	6.7974179472542	3.4468971223470515
10	24.310401013222275	54.70845592228881	10.287071940168117	10.694071124320802
11	16.17365298523848	40.92803209769829	28.987771535017874	13.91054338204535
12	19.50545323438392	36.7667485595557	27.929250090713904	15.798548115346481
13	19.4116192373561	38.435884339198346	24.42272446779252	17.729771955653035
14	14.471005063800185	41.65096988760721	26.41034115360348	17.467683894989126
15	13.163107060892717	44.03241201907188	25.84017250663887	16.964308413396537
16	15.310657554445676	44.87067780040168	22.814141661601326	17.004522983551315
17	14.937170512088638	43.342986370958606	23.67667485595557	18.043168260997184
18	16.34190704887457	42.211200451142766	23.457574784077803	17.98931771590486
19	16.234668195128492	44.91805703535415	23.756641759826568	15.090633009690787
20	17.219231809262755	43.51863621761167	23.783220355043806	15.478911618081764
21	16.054395984089822	45.139699407644	22.639416287825387	16.166488320440788
22	16.267024745827737	43.56000637886285	23.385697017881615	16.787271857427793
23	16.06040505779111	43.31779448505706	21.169042176763835	19.452758280388004
24	12.552261607334767	44.502968713526656	23.64270047772136	19.302069201417215
25	14.938788339623601	45.7006233258374	22.068323167983657	17.292265166555346
26	16.809459206478707	45.45170900367247	20.61366509583317	17.125166694015657
27	13.281670707383533	45.725815211738954	21.604006665449443	19.388507415428066
28	17.04681761767962	43.802218272668654	23.328610817719373	15.822353291932355
29	17.331786382052282	43.54336586707467	21.519186278973557	17.60566147189949
30	16.733883548774035	45.93312825443343	20.301424381585427	17.031563815207114
31	18.527592048608785	46.39513357477483	18.290233637407873	16.78704073920851
32	18.67342764497468	45.90886084140899	18.56457096369364	16.853140549922692
33	17.057449055766515	44.15097566556269	21.545764874190798	17.245810404479993
34	18.661871734010663	43.47772829279905	20.201581310856316	17.65881866233397
35	20.41998802807624	43.75021667333058	20.139872746308466	15.689922552284719
36	18.843068417926453	44.81590278243225	21.92456763559128	14.416461164050023
37	17.338257692192133	46.18874500495749	20.075390763129246	16.39760653972113
38	17.850415666117375	45.03962521869562	23.023534768269318	14.086424346917692
39	18.488301951331128	42.32028825064308	22.87931699943838	16.312092798587404
40	21.8808862921473	41.98170005939738	20.349034734757176	15.788378913698145
41	20.13178360863365	39.3030398979382	23.938531798400202	16.626644695027952
42	22.30660605206169	40.52981540587826	20.63492797200696	16.528650570053088
43	17.992784489194065	42.118059808772784	22.48710938131964	17.402046320713506
44	19.107005424344607	40.465102304479764	20.448877805486283	19.979014465689346
45	18.86086452081104	42.352644801342336	21.972871343420874	16.813619334425752
46	17.08772554249224	41.68032190145581	24.61016134362888	16.62179121242307
47	18.314501050432305	40.01858190483014	23.33253982744714	18.334377217290417
48	19.138899738605293	38.364468809440716	25.758125538794346	16.738505913159642
49	19.157620314367	38.45090702345156	23.467743985726138	18.923728676455294
50	17.96227688424906	37.40856385449721	23.176303911213626	21.4528553500401
51	17.488253416505074	37.403248135453765	25.371926994376892	19.736571453664265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34934.0
1	26243.5
2	17553.0
3	10732.0
4	3911.0
5	3112.0
6	2313.0
7	2458.0
8	2603.0
9	3138.0
10	3673.0
11	4129.0
12	4585.0
13	4879.5
14	5174.0
15	5184.0
16	5194.0
17	5229.5
18	5265.0
19	5343.0
20	5421.0
21	5099.0
22	4777.0
23	4765.0
24	4753.0
25	4975.0
26	5140.5
27	5084.0
28	6637.5
29	8191.0
30	8187.5
31	8184.0
32	9402.0
33	10620.0
34	11769.0
35	12918.0
36	13990.5
37	15063.0
38	15392.5
39	15722.0
40	16679.0
41	17636.0
42	19305.5
43	20975.0
44	24784.5
45	28594.0
46	30486.0
47	32378.0
48	35614.0
49	38850.0
50	35138.0
51	31426.0
52	28185.5
53	24945.0
54	20866.0
55	16787.0
56	13900.5
57	11014.0
58	9701.0
59	8388.0
60	7548.5
61	6709.0
62	5585.0
63	4461.0
64	3804.5
65	3148.0
66	2628.0
67	2108.0
68	1905.5
69	1703.0
70	1289.5
71	876.0
72	788.5
73	701.0
74	504.5
75	237.5
76	167.0
77	135.0
78	103.0
79	138.0
80	173.0
81	100.0
82	27.0
83	23.5
84	20.0
85	24.5
86	29.0
87	17.5
88	6.0
89	5.5
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.380785040453034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33040343295356	25.813495740869342
2	9.704070002370905	6.4785894953330345
3	3.656394341720507	3.66159940632303
4	1.878497584214267	2.508228962306671
5	1.1861932153553367	1.979803036911015
6	0.8719525039554624	1.7463875460013236
7	0.6571209149980377	1.5354648406394713
8	0.4993701545573096	1.333549422791629
9	0.41535232469381195	1.247830799999093
>10	3.2049631607901254	21.305739978495467
>50	0.39362578773704465	9.012602690698515
>100	0.19226836878807685	10.19842847394007
>500	0.006292419342155242	1.3926043000595083
>1k	0.0013983154093678315	1.6019500445128985
>5k	0.0013983154093678315	2.995721266224878
>10k+	6.991577046839158E-4	7.18800399489405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30799	7.118210035615317	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6763	1.563052516992967	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6073	1.4035809456895296	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	3593	0.8304077618742763	Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3271	0.7559876952660055	No Hit
TGCCATGGGAGCAAGAGAGGCAATTTTTTAGAGTTTCTTTTTATGAACTGT	863	0.19945502323893693	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	843	0.19483265885333007	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	759	0.17541872843378117	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	752	0.17380090089881875	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	633	0.1462978328044578	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	545	0.12595942950778752	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	542	0.1252660748499465	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	517	0.1194881193679379	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT	513	0.11856364649081652	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	480	0.11093674525456515	No Hit
AATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	473	0.10931891771960274	No Hit
TGATTACGGGAACAAAACAAAACAAAACGAAAGACAAAAAGACAAAACAAA	470	0.10862556306176173	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.311182192803441E-4	0.0	0.0	0.9697720481003238	0.0
2	2.311182192803441E-4	0.0	0.0	1.2582075857621933	0.0
3	2.311182192803441E-4	0.0	0.0	1.5313893209515599	0.0
4	2.311182192803441E-4	0.0	0.0	2.970793590629543	0.0
5	2.311182192803441E-4	0.0	0.0	3.1889691896301877	0.0
6	2.311182192803441E-4	0.0	0.0	4.451105785120147	0.0
7	2.311182192803441E-4	0.0	0.0	5.469412659269343	0.0
8	2.311182192803441E-4	0.0	0.0	6.137806549428098	0.0
9	2.311182192803441E-4	0.0	0.0	7.4570293450803025	0.0
10	2.311182192803441E-4	0.0	0.0	9.448806158838307	0.0
11	2.311182192803441E-4	0.0	0.0	11.420013451080361	0.0
12	2.311182192803441E-4	0.0	0.0	12.436933615913876	0.0
13	2.311182192803441E-4	0.0	0.0	12.854102001714898	0.0
14	2.311182192803441E-4	0.0	0.0	13.247696329149322	0.0
15	2.311182192803441E-4	0.0	0.0	13.57495972765029	0.0
16	2.311182192803441E-4	0.0	0.0	14.250287164387457	0.0
17	2.311182192803441E-4	0.0	0.0	15.198102981656147	0.0
18	2.311182192803441E-4	0.0	0.0	16.42326066206125	0.0
19	2.311182192803441E-4	0.0	0.0	16.915311350909104	0.0
20	2.311182192803441E-4	0.0	0.0	17.478315333076022	0.0
21	2.311182192803441E-4	0.0	0.0	18.037852541953736	0.0
22	2.311182192803441E-4	0.0	0.0	18.657942724282897	0.0
23	2.311182192803441E-4	0.0	0.0	19.24428964659713	0.0
24	2.311182192803441E-4	0.0	0.0	19.656373431573986	0.0
25	2.311182192803441E-4	0.0	0.0	20.00258852405594	0.0
26	2.311182192803441E-4	0.0	0.0	20.334012050503954	0.0
27	2.311182192803441E-4	0.0	0.0	20.73500216095535	0.0
28	2.311182192803441E-4	0.0	0.0	21.103635720707498	0.0
29	2.311182192803441E-4	0.0	0.0	21.48821643758999	0.0
30	2.311182192803441E-4	0.0	0.0	21.97703147136792	0.0
31	2.311182192803441E-4	0.0	0.0	22.344509440023668	0.0
32	2.311182192803441E-4	0.0	0.0	22.672235074963194	0.0
33	2.311182192803441E-4	0.0	0.0	23.002734128534087	0.0
34	4.622364385606882E-4	0.0	0.0	23.35287823074381	0.0
35	4.622364385606882E-4	0.0	0.0	23.71134258884762	0.0
36	4.622364385606882E-4	0.0	0.0	24.049237425435486	0.0
37	4.622364385606882E-4	0.0	0.0	24.39429692682104	0.0
38	4.622364385606882E-4	0.0	0.0	24.775179752195044	0.0
39	4.622364385606882E-4	0.0	0.0	25.124861617966207	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTC	20	7.0284033E-4	45.000004	20
GTCGCTC	40	6.7975634E-9	45.000004	33
TCGTTAA	20	7.0284033E-4	45.000004	16
CGAACAG	30	2.1623528E-6	45.000004	1
CTCGTCG	40	6.7975634E-9	45.000004	37
ATCTACG	20	7.0284033E-4	45.000004	1
CGTGCTT	40	6.7975634E-9	45.000004	45
CGAAACG	20	7.0284033E-4	45.000004	1
ACGTTAG	20	7.0284033E-4	45.000004	1
TAGACAG	20	7.0284033E-4	45.000004	1
ACGCAAC	20	7.0284033E-4	45.000004	37
TGGCGCT	20	7.0284033E-4	45.000004	15
TCTTACG	20	7.0284033E-4	45.000004	1
CGCGCAG	40	6.7975634E-9	45.000004	1
TTCGTTA	20	7.0284033E-4	45.000004	15
GTTAGCG	20	7.0284033E-4	45.000004	1
TTTCCGG	20	7.0284033E-4	45.000004	2
ACGGGCG	20	7.0284033E-4	45.000004	5
GCACCGT	20	7.0284033E-4	45.000004	24
CTGCGAC	40	6.7975634E-9	45.000004	30
>>END_MODULE