FastQCFastQC Report
Sat 18 Jun 2016
SRR3553397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797097
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223962.80969568321045No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48410.6073288445446414No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42150.5287938607220953No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT36100.45289343705973045No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24470.30698898628397797No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGT17020.2135248282204048No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT12790.16045725927961088No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC11840.14853901093593377No Hit
TGCACTGGGGAAAACCTGTTCTGTGCTGGTTTATTTTCTGGATTTGTTTTC10450.1311007317804483No Hit
AAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT10270.1288425373574358No Hit
GTACATGGGGAGTCAGACAAATCATCGTCCTGAATGCTTCATTTAAATATA9910.12432614851141079No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT8630.10826787705887742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAG551.8189894E-1245.0000041
CGATAGG1150.045.0000042
TACGTTG551.8189894E-1245.0000041
TAATCCG551.8189894E-1245.0000041
CGGGTTA406.8102963E-945.06
CGGCGTA207.0319837E-445.022
AGCGTAA351.2113014E-745.032
GTACCGT253.8897255E-545.013
CGAACAT207.0319837E-445.023
ACGCCTA253.8897255E-545.032
ATTAGGT253.8897255E-545.044
GTATTCG207.0319837E-445.09
ACGTTTC207.0319837E-445.039
ACGTTCC207.0319837E-445.019
CGTGCGT253.8897255E-545.023
TAGCGTA351.2113014E-745.031
CGCATCT253.8897255E-545.042
TGTGGCG253.8897255E-545.017
CGCATAC207.0319837E-445.030
CTCCCGT207.0319837E-445.023