FastQCFastQC Report
Sat 18 Jun 2016
SRR3553394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029309
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198511.928575384068341No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45120.438352331515609No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35210.3420741487735947No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT32420.3149685857211003No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA27590.2680439012968895No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21360.2075178590685596No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19740.19177914503807894No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG14200.13795662915606488No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10300.1000671324160189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTT351.2117562E-745.00000441
AACGGTT650.045.0000047
CGTTACG351.2117562E-745.0000041
ACGATAG700.045.0000041
ATATCCC351.2117562E-745.00000415
GGTGCGT351.2117562E-745.00000440
ACGTCAT351.2117562E-745.00000432
CACGTTA351.2117562E-745.00000416
CGACTCG351.2117562E-745.00000416
GCTCGAA351.2117562E-745.00000429
TCGCAAC207.0329447E-445.016
CGTATAG406.8139343E-945.01
CGACGGT207.0329447E-445.028
CGAAAAC502.1827873E-1145.011
CGACGCA207.0329447E-445.039
CTATCGA207.0329447E-445.034
ACCGCCG207.0329447E-445.044
CGCATTT800.045.023
ACATTCG207.0329447E-445.010
TGAACGT207.0329447E-445.09