##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1029309
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624803630396702	31.0	31.0	34.0	30.0	34.0
2	31.839271783303168	33.0	31.0	34.0	30.0	34.0
3	32.01232477322165	33.0	31.0	34.0	30.0	34.0
4	35.72654275829707	37.0	35.0	37.0	33.0	37.0
5	35.45627309194809	37.0	35.0	37.0	33.0	37.0
6	34.041970875606836	37.0	35.0	37.0	32.0	37.0
7	34.905751334147475	37.0	35.0	37.0	32.0	37.0
8	35.633465752266815	37.0	35.0	37.0	33.0	37.0
9	37.32103381977618	39.0	37.0	39.0	34.0	39.0
10	36.69929535251319	38.0	37.0	39.0	32.0	39.0
11	36.722594478431645	39.0	37.0	39.0	32.0	39.0
12	36.70602511004956	39.0	35.0	39.0	32.0	39.0
13	36.69063031606641	39.0	35.0	39.0	32.0	39.0
14	37.87429333659766	40.0	37.0	41.0	33.0	41.0
15	37.87500643635682	40.0	37.0	41.0	33.0	41.0
16	37.795462781341655	40.0	37.0	41.0	33.0	41.0
17	37.75826889690074	39.0	37.0	41.0	33.0	41.0
18	37.64603049230114	39.0	36.0	41.0	33.0	41.0
19	37.61142086584301	39.0	36.0	41.0	32.0	41.0
20	37.489220438177455	39.0	36.0	41.0	32.0	41.0
21	37.43358311255415	39.0	36.0	41.0	32.0	41.0
22	37.42104946133765	39.0	36.0	41.0	32.0	41.0
23	37.28895696044628	39.0	36.0	41.0	32.0	41.0
24	37.10364137494183	39.0	35.0	40.0	31.0	41.0
25	37.15778740883447	39.0	35.0	40.0	32.0	41.0
26	37.05669143085313	39.0	35.0	40.0	31.0	41.0
27	37.02583383609781	39.0	35.0	40.0	31.0	41.0
28	36.98031106305298	39.0	35.0	40.0	31.0	41.0
29	36.81081579972584	39.0	35.0	40.0	31.0	41.0
30	36.82448807889565	39.0	35.0	40.0	31.0	41.0
31	36.72893368269392	39.0	35.0	40.0	31.0	41.0
32	36.633982603863366	39.0	35.0	40.0	30.0	41.0
33	36.54133404060394	39.0	35.0	40.0	30.0	41.0
34	36.470466108816694	39.0	35.0	40.0	30.0	41.0
35	36.362541277692124	39.0	35.0	40.0	30.0	41.0
36	36.246950138393814	38.0	35.0	40.0	30.0	41.0
37	36.23658298917041	38.0	35.0	40.0	30.0	41.0
38	36.13512657520725	38.0	35.0	40.0	30.0	41.0
39	36.084615018424984	38.0	35.0	40.0	30.0	41.0
40	35.92881632240658	38.0	35.0	40.0	29.0	41.0
41	35.82257611659861	38.0	35.0	40.0	29.0	41.0
42	35.7209817460063	38.0	35.0	40.0	28.0	41.0
43	35.60707620355015	38.0	34.0	40.0	28.0	41.0
44	35.49795931056661	38.0	34.0	40.0	27.0	41.0
45	35.3509189174485	38.0	34.0	40.0	27.0	41.0
46	35.17818070181063	38.0	34.0	40.0	26.0	41.0
47	35.09171201262206	38.0	34.0	40.0	26.0	41.0
48	34.945924887473055	37.0	34.0	40.0	26.0	41.0
49	34.78630032380947	37.0	33.0	40.0	26.0	41.0
50	34.69709581865116	37.0	33.0	40.0	26.0	41.0
51	33.55794032695721	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	6.0
11	20.0
12	9.0
13	13.0
14	35.0
15	80.0
16	136.0
17	268.0
18	522.0
19	977.0
20	1635.0
21	2396.0
22	3401.0
23	4465.0
24	5707.0
25	7250.0
26	8659.0
27	10776.0
28	13380.0
29	17299.0
30	22013.0
31	28685.0
32	37013.0
33	49035.0
34	70067.0
35	95061.0
36	106555.0
37	142627.0
38	198525.0
39	202666.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.112725138903865	32.616153166833286	22.15058840445386	26.12053328980899
2	23.187011869127737	32.24337881044468	20.375708363572066	24.193900956855522
3	20.955806273917744	33.59943418351535	20.19947362745298	25.245285915113925
4	20.979220039851977	34.566782181055444	19.1052443921116	25.348753386980977
5	18.891994532254163	37.34427659721231	17.113325541698362	26.65040332883517
6	17.83060286075416	43.66929658635065	17.736753491905734	20.76334706098946
7	83.5958881152307	8.983793982176392	3.86968344782762	3.550634454765284
8	85.71653410200435	7.952228145289704	3.400825213808487	2.9304125388974542
9	78.17292960617269	12.373446652074353	5.583163073479393	3.87046066827357
10	32.68202259962752	44.27426555096672	11.322838914261899	11.720872935143868
11	23.127360199901098	32.71116836635063	27.36311447777101	16.798356955977262
12	25.739695271293655	27.764160227881035	28.53147111314484	17.964673387680474
13	24.15503993455804	28.705179882814587	26.488061408187434	20.65171877443994
14	19.75694373604039	31.1644996789108	26.60503308530286	22.473523499745944
15	18.40108266808121	34.429214162122356	26.835576100082676	20.33412706971376
16	21.48655068594562	33.09200638486596	25.92982282288409	19.491620106304328
17	20.638408874303053	33.77401732618679	25.9574141487153	19.630159650794855
18	21.523954419906946	32.81094404109942	25.515370020081434	20.149731518912205
19	20.872255075978156	34.618273035599614	25.147258986368527	19.362212902053706
20	22.90750396625309	32.82639129746266	25.59280060700917	18.673304129275074
21	23.269785846621375	32.792582208063855	25.875806001890588	18.061825943424182
22	22.690950919500363	31.056660342035286	25.5050718491726	20.74731688929175
23	21.365692906600447	32.19198510845626	25.50264303527901	20.93967894966429
24	19.862645716689546	33.027011325073424	27.0174456844349	20.092897273802134
25	21.083367579609234	34.12240639108373	24.76593520507447	20.028290824232567
26	22.040125948573266	34.12881845976281	23.977250757547054	19.85380483411687
27	19.91258213034181	33.47517606471915	25.219054725063124	21.393187079875915
28	20.57856289996493	33.66005737829943	25.954693877154476	19.80668584458117
29	19.75402915936808	33.62313940711682	25.11898759264711	21.503843840868
30	21.03615143751779	34.2779476328294	24.21566312934211	20.470237800310695
31	21.970661871216514	32.9609475871677	23.701046041567693	21.36734450004809
32	22.048481068367227	32.40805239243026	25.149882105373607	20.39358443382891
33	20.850687208603052	33.38501849298899	24.498959981890764	21.265334316517198
34	21.343833581558115	32.550380886594795	25.43619068715031	20.669594844696782
35	22.202467869221003	31.12476428361163	25.887464308579833	20.785303538587538
36	21.308275746155918	34.30252722943256	25.564432060732006	18.824764963679517
37	20.686499389396186	34.26541495313846	24.213040010337032	20.835045647128318
38	21.120091245680356	32.23434362276051	26.56811511412025	20.077450017438885
39	21.654333149715	31.315766208203755	25.787494328719557	21.242406313361684
40	22.28805927083121	32.54076278357616	24.759717441506876	20.411460504085753
41	20.529792316981588	31.888771981980145	26.818768707939018	20.762666993099256
42	21.613237618635413	30.789102203517118	25.93953807845846	21.658122099389008
43	20.46489440974479	31.949880939542936	26.495250697312468	21.089973953399806
44	21.99096675536695	31.263983895992357	24.234316420044905	22.510732928595786
45	20.96095535937216	32.456725822857855	25.812656840657176	20.7696619771128
46	20.331601103264425	32.299435835108795	26.447451639886566	20.921511421740217
47	21.213940614528777	31.969408603247423	26.685475401458646	20.131175380765153
48	20.604211174681268	30.792794000635375	27.962059983930963	20.64093484075239
49	20.61713246459518	32.1040620455082	25.81061663698656	21.468188852910057
50	20.191409965326255	29.857700651602194	27.047271519048216	22.90361786402334
51	21.077149816041636	28.341635019221634	27.41839428198918	23.16282088274755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22773.0
1	17076.0
2	11379.0
3	7160.0
4	2941.0
5	2391.5
6	1842.0
7	2197.5
8	2553.0
9	2838.0
10	3123.0
11	3601.0
12	4079.0
13	4670.5
14	5262.0
15	4893.0
16	4524.0
17	4869.0
18	5214.0
19	5237.0
20	5260.0
21	5620.5
22	5981.0
23	8590.0
24	11199.0
25	9668.0
26	10949.5
27	13762.0
28	17666.5
29	21571.0
30	23418.0
31	25265.0
32	28249.0
33	31233.0
34	33982.0
35	36731.0
36	38663.5
37	40596.0
38	43132.5
39	45669.0
40	49119.0
41	52569.0
42	55653.0
43	58737.0
44	62486.0
45	66235.0
46	68783.0
47	71331.0
48	74515.0
49	77699.0
50	75541.5
51	73384.0
52	67052.5
53	60721.0
54	55726.0
55	50731.0
56	48192.0
57	45653.0
58	40598.0
59	35543.0
60	33843.0
61	32143.0
62	29210.0
63	26277.0
64	23015.0
65	19753.0
66	18071.0
67	16389.0
68	13716.0
69	11043.0
70	9776.5
71	8510.0
72	6643.5
73	4777.0
74	3795.5
75	2619.5
76	2425.0
77	2076.5
78	1728.0
79	1247.5
80	767.0
81	642.5
82	518.0
83	345.5
84	173.0
85	181.0
86	189.0
87	131.5
88	74.0
89	44.5
90	15.0
91	13.0
92	11.0
93	8.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1029309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.554893462164056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36643249859752	17.2243674909141
2	9.681206981464777	4.367171841041938
3	3.686848093046617	2.4946939784954747
4	1.898054241482581	1.7124164480819288
5	1.1896029707854026	1.3415684134169308
6	0.8406442177926767	1.1376384463138836
7	0.6126917569099355	0.9673438111574797
8	0.47581377972059546	0.8585543287542102
9	0.3929961523848561	0.7977587713272739
>10	3.030618441676117	15.29510128205487
>50	0.9396376581100142	15.312723162291903
>100	0.877083252331169	33.87103142356444
>500	0.004405239840940075	0.6010269177752001
>1k	0.0035241918727520606	2.046213976634563
>5k	0.0	0.0
>10k+	4.405239840940076E-4	1.972389708175814
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19851	1.928575384068341	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4512	0.438352331515609	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3521	0.3420741487735947	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT	3242	0.3149685857211003	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2759	0.2680439012968895	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2136	0.2075178590685596	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1974	0.19177914503807894	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1420	0.13795662915606488	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1030	0.1000671324160189	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.91457667231123E-4	0.0	0.0	0.39560520698837764	0.0
2	2.91457667231123E-4	0.0	0.0	0.5071363409821541	0.0
3	2.91457667231123E-4	0.0	0.0	0.6474246314760679	0.0
4	2.91457667231123E-4	0.0	0.0	1.2550167150972158	0.0
5	2.91457667231123E-4	0.0	0.0	1.3382764553695732	0.0
6	2.91457667231123E-4	0.0	0.0	1.9045787027996453	0.0
7	2.91457667231123E-4	0.0	0.0	2.345359848208847	0.0
8	2.91457667231123E-4	0.0	0.0	2.7538863451111375	0.0
9	2.91457667231123E-4	0.0	0.0	3.3435052059196995	0.0
10	2.91457667231123E-4	0.0	0.0	4.215838003942451	0.0
11	2.91457667231123E-4	0.0	0.0	5.083118869066529	0.0
12	2.91457667231123E-4	0.0	0.0	5.51603065746049	0.0
13	2.91457667231123E-4	0.0	0.0	5.718010820851659	0.0
14	2.91457667231123E-4	0.0	0.0	5.903280744654909	0.0
15	2.91457667231123E-4	0.0	0.0	6.068537241974956	0.0
16	2.91457667231123E-4	0.0	0.0	6.414400340422556	0.0
17	2.91457667231123E-4	0.0	0.0	6.904049221370842	0.0
18	2.91457667231123E-4	0.0	0.0	7.488130386502013	0.0
19	2.91457667231123E-4	0.0	0.0	7.75481415201849	0.0
20	2.91457667231123E-4	0.0	0.0	8.032184698666775	0.0
21	2.91457667231123E-4	0.0	0.0	8.35220521728655	0.0
22	2.91457667231123E-4	0.0	0.0	8.717693131994377	0.0
23	2.91457667231123E-4	0.0	0.0	9.093576370166781	0.0
24	2.91457667231123E-4	0.0	0.0	9.36103735612921	0.0
25	2.91457667231123E-4	0.0	0.0	9.597215219142162	0.0
26	2.91457667231123E-4	0.0	0.0	9.800944128536717	0.0
27	2.91457667231123E-4	0.0	0.0	10.034596025100335	0.0
28	2.91457667231123E-4	0.0	0.0	10.27767171957109	0.0
29	2.91457667231123E-4	0.0	0.0	10.533085788621298	0.0
30	2.91457667231123E-4	0.0	0.0	10.849608815234298	0.0
31	2.91457667231123E-4	0.0	0.0	11.092587357149311	0.0
32	2.91457667231123E-4	0.0	0.0	11.328570915050777	0.0
33	2.91457667231123E-4	0.0	0.0	11.571355151854302	0.0
34	2.91457667231123E-4	0.0	0.0	11.81239064265444	0.0
35	2.91457667231123E-4	0.0	0.0	12.086555154963184	0.0
36	2.91457667231123E-4	0.0	0.0	12.340706240788723	0.0
37	2.91457667231123E-4	0.0	0.0	12.600395022291654	0.0
38	2.91457667231123E-4	0.0	0.0	12.879805772610558	0.0
39	2.91457667231123E-4	0.0	0.0	13.156204793701406	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTT	35	1.2117562E-7	45.000004	41
AACGGTT	65	0.0	45.000004	7
CGTTACG	35	1.2117562E-7	45.000004	1
ACGATAG	70	0.0	45.000004	1
ATATCCC	35	1.2117562E-7	45.000004	15
GGTGCGT	35	1.2117562E-7	45.000004	40
ACGTCAT	35	1.2117562E-7	45.000004	32
CACGTTA	35	1.2117562E-7	45.000004	16
CGACTCG	35	1.2117562E-7	45.000004	16
GCTCGAA	35	1.2117562E-7	45.000004	29
TCGCAAC	20	7.0329447E-4	45.0	16
CGTATAG	40	6.8139343E-9	45.0	1
CGACGGT	20	7.0329447E-4	45.0	28
CGAAAAC	50	2.1827873E-11	45.0	11
CGACGCA	20	7.0329447E-4	45.0	39
CTATCGA	20	7.0329447E-4	45.0	34
ACCGCCG	20	7.0329447E-4	45.0	44
CGCATTT	80	0.0	45.0	23
ACATTCG	20	7.0329447E-4	45.0	10
TGAACGT	20	7.0329447E-4	45.0	9
>>END_MODULE