##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553393_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1020836
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61014697757524	31.0	31.0	34.0	30.0	34.0
2	31.833977250018613	33.0	31.0	34.0	30.0	34.0
3	32.00241272839124	33.0	31.0	34.0	30.0	34.0
4	35.72893099381292	37.0	35.0	37.0	33.0	37.0
5	35.47786226191083	37.0	35.0	37.0	33.0	37.0
6	32.58580614320028	37.0	35.0	37.0	26.0	37.0
7	34.181028098538846	37.0	35.0	37.0	26.0	37.0
8	35.336306713321235	37.0	35.0	37.0	32.0	37.0
9	37.19477075651721	39.0	37.0	39.0	34.0	39.0
10	36.66322014505758	38.0	37.0	39.0	32.0	39.0
11	36.73014372533884	39.0	37.0	39.0	32.0	39.0
12	36.722330521259046	39.0	35.0	39.0	32.0	39.0
13	36.72243337813322	39.0	37.0	39.0	32.0	39.0
14	37.887123886696784	40.0	37.0	41.0	33.0	41.0
15	37.9264348044152	40.0	37.0	41.0	33.0	41.0
16	37.867901406298365	40.0	37.0	41.0	33.0	41.0
17	37.854707318315576	39.0	37.0	41.0	33.0	41.0
18	37.728696871975515	39.0	37.0	41.0	33.0	41.0
19	37.69623034454114	39.0	36.0	41.0	33.0	41.0
20	37.560740412759735	39.0	36.0	41.0	32.0	41.0
21	37.497857638249435	39.0	36.0	41.0	32.0	41.0
22	37.44984600856553	39.0	36.0	41.0	32.0	41.0
23	37.33025774953078	39.0	36.0	41.0	32.0	41.0
24	37.19450528782292	39.0	35.0	41.0	32.0	41.0
25	37.20857708779863	39.0	35.0	41.0	32.0	41.0
26	37.12675101583408	39.0	35.0	40.0	32.0	41.0
27	37.06853990258964	39.0	35.0	40.0	31.0	41.0
28	37.05214941479336	39.0	35.0	40.0	31.0	41.0
29	36.84468122205722	39.0	35.0	40.0	31.0	41.0
30	36.870714786704234	39.0	35.0	40.0	31.0	41.0
31	36.49390303633493	39.0	35.0	40.0	30.0	41.0
32	36.58233447879973	39.0	35.0	40.0	30.0	41.0
33	36.53035845130854	39.0	35.0	40.0	30.0	41.0
34	36.497921311552496	39.0	35.0	40.0	30.0	41.0
35	36.47341590617886	39.0	35.0	40.0	30.0	41.0
36	36.33940319502839	38.0	35.0	40.0	30.0	41.0
37	36.29739448843889	38.0	35.0	40.0	30.0	41.0
38	36.200356374579265	38.0	35.0	40.0	30.0	41.0
39	36.12620146624923	38.0	35.0	40.0	30.0	41.0
40	35.99042745357726	38.0	35.0	40.0	29.0	41.0
41	35.95031131347249	38.0	35.0	40.0	29.0	41.0
42	35.82881285534601	38.0	35.0	40.0	29.0	41.0
43	35.73604966909474	38.0	35.0	40.0	28.0	41.0
44	35.590364172109915	38.0	34.0	40.0	28.0	41.0
45	35.42831855459643	38.0	34.0	40.0	27.0	41.0
46	35.15806946463487	38.0	34.0	40.0	26.0	41.0
47	35.13720813137468	38.0	34.0	40.0	26.0	41.0
48	35.08768597502439	37.0	34.0	40.0	26.0	41.0
49	34.97764871144826	37.0	34.0	40.0	26.0	41.0
50	34.85300087379363	37.0	33.0	40.0	26.0	41.0
51	33.784763664290836	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	12.0
11	14.0
12	14.0
13	16.0
14	40.0
15	69.0
16	121.0
17	273.0
18	534.0
19	925.0
20	1471.0
21	2360.0
22	3259.0
23	4376.0
24	5636.0
25	6835.0
26	8312.0
27	10668.0
28	13219.0
29	16755.0
30	21419.0
31	28076.0
32	36746.0
33	49571.0
34	71012.0
35	95145.0
36	108341.0
37	144936.0
38	194319.0
39	196312.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.1229541277933	32.59720464403685	22.127060566045866	26.152780662123988
2	23.24839641235223	32.201450575802575	20.272012350661615	24.278140661183578
3	20.934214702459553	33.52898996508743	20.17454321751976	25.36225211493325
4	20.992402305561324	34.63896257577123	19.02881559819599	25.33981952047146
5	18.904701636697766	37.430204263956206	17.13164504386601	26.53344905548002
6	17.037408555340917	46.19694054676755	16.922502733054085	19.843148164837444
7	83.65829574975804	8.990278556007038	3.86124705633422	3.490178637900701
8	85.59033968237797	8.037627983339146	3.355387153274375	3.016645181008507
9	78.27466899678302	12.391608446410588	5.510581523378877	3.8231410334275044
10	32.67390648448919	44.40605542907969	11.304460265899714	11.615577820531406
11	23.214796500123427	32.63021680269896	27.341904086454633	16.813082610722976
12	25.77603062587918	27.794964127440647	28.49409699501193	17.93490825166824
13	24.096818685861393	28.653182293727887	26.567244885564378	20.68275413484634
14	19.71237299625013	31.196685853555323	26.641889588533317	22.449051561661225
15	18.43263756372228	34.470277302132764	26.818215658538687	20.278869475606268
16	21.585543613273828	33.0393912440392	25.945205694156552	19.429859448530422
17	20.64650933156746	33.8463768910971	25.948536297701104	19.55857747963434
18	21.528727435160985	32.86355496867274	25.490970146037167	20.11674745012911
19	20.91814943830351	34.618783036648395	25.102073202747555	19.360994322300545
20	22.924250320325694	32.88324471315667	25.594806609484777	18.59769835703286
21	23.30002076729269	32.91586503610766	25.832357009353117	17.951757187246532
22	22.65447143321748	31.13232683800336	25.493712996015034	20.719488732764127
23	21.322034097543582	32.21790767566974	25.584912757778916	20.875145469007755
24	19.88370316093868	33.045464697561606	27.076729268952114	19.994102872547597
25	21.01395326967309	34.23752688972568	24.839347358439554	19.90917248216168
26	21.937999835429	34.1915841525965	24.070173857505026	19.800242154469476
27	20.05493536669945	33.41339843030614	25.257044226496717	21.27462197649769
28	20.47224039904549	33.76869546136696	25.966266863629418	19.792797275958137
29	19.7339239603619	33.63909579991301	25.125485386487156	21.501494853237936
30	21.002100239411618	34.26329008773202	24.30919364129008	20.425416031566286
31	22.007158838442216	32.95024861975871	23.652672907303426	21.389919634495648
32	22.084644350316797	32.496404907350446	25.214138216128738	20.204812526204012
33	20.985055385977766	33.429071858751065	24.486597259501035	21.099275495770133
34	21.324874906449224	32.6661677291945	25.365484759550018	20.643472604806256
35	22.142244199851884	31.128604398747694	25.937173062078532	20.79197833932189
36	21.23308739111865	34.44794266659875	25.553565900889076	18.765404041393523
37	20.672958242068265	34.267012526987685	24.383544467475676	20.676484763468373
38	21.14267130077701	32.34260939073465	26.523359285918602	19.991360022569737
39	21.647061819920143	31.357926248682453	25.854397768103787	21.140614163293613
40	22.308676418151396	32.5734985835139	24.776359767876524	20.341465230458173
41	20.473317947251076	32.02394899866384	26.852501283261955	20.65023177082313
42	21.511976458510475	30.912311086207772	25.994674952685838	21.581037502595912
43	20.45313840812824	32.12278955679463	26.44264112942725	20.98143090564988
44	22.0408567095988	31.295526411686108	24.268540686261066	22.395076192454027
45	20.985741098472232	32.66577589348338	25.708438965710457	20.64004404233393
46	20.36448557848665	32.36004607987963	26.48740835942306	20.788059982210658
47	21.09594489222559	31.99994906135755	26.726722019991456	20.1773840264254
48	20.57284421787633	30.909862113013254	27.954147385084383	20.563146284026036
49	20.56667280542614	32.242593325470494	25.811001963096913	21.379731906006448
50	20.06404554698306	30.008933854213605	27.213577891061835	22.7134427077415
51	21.054116430063203	28.417199236704032	27.410181459117823	23.11850287411494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22923.0
1	17252.5
2	11582.0
3	7272.0
4	2962.0
5	2406.5
6	1851.0
7	2148.0
8	2445.0
9	2746.5
10	3048.0
11	3508.0
12	3968.0
13	4545.0
14	5122.0
15	4925.0
16	4728.0
17	4896.5
18	5065.0
19	5133.5
20	5202.0
21	5682.5
22	6163.0
23	8722.5
24	11282.0
25	9789.5
26	11016.5
27	13736.0
28	17820.0
29	21904.0
30	23440.5
31	24977.0
32	28245.5
33	31514.0
34	34141.5
35	36769.0
36	38773.0
37	40777.0
38	42985.0
39	45193.0
40	48857.0
41	52521.0
42	55500.0
43	58479.0
44	62026.5
45	65574.0
46	67908.0
47	70242.0
48	73460.0
49	76678.0
50	74450.0
51	72222.0
52	65749.5
53	59277.0
54	54739.5
55	50202.0
56	47594.5
57	44987.0
58	40053.0
59	35119.0
60	33566.5
61	32014.0
62	28948.5
63	25883.0
64	22825.5
65	19768.0
66	17971.0
67	16174.0
68	13416.5
69	10659.0
70	9530.5
71	8402.0
72	6484.5
73	4567.0
74	3681.0
75	2532.0
76	2269.0
77	2033.0
78	1797.0
79	1273.5
80	750.0
81	641.5
82	533.0
83	341.5
84	150.0
85	177.5
86	205.0
87	121.0
88	37.0
89	23.0
90	9.0
91	6.5
92	4.0
93	7.0
94	10.0
95	5.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1020836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.805587096369205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40621015388571	17.196828932715604
2	9.900033429786538	4.515521492799273
3	3.6669179102406626	2.5087864733168876
4	1.998772164640072	1.8233269074599032
5	1.2994993371869241	1.4817922657945226
6	0.9045374681133457	1.2377104806592913
7	0.6855781092971542	1.0944507898058264
8	0.5349527212824352	0.9759928702117039
9	0.43491558963058513	0.8926654823000169
>10	3.4304007521156414	16.876146532204274
>50	0.8845291288914144	14.463491599890233
>100	0.846695287013662	32.47487159758135
>500	0.0030441022131975524	0.43974219167407447
>1k	0.00347897395794006	2.0284273897857115
>5k	0.0	0.0
>10k+	4.348717447425075E-4	1.9902449938013815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20068	1.9658397627042932	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4493	0.440129462518955	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3564	0.34912561861062896	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT	2949	0.2888808780254615	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2887	0.28280742450305435	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2078	0.20355865192841943	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2051	0.20091376087833895	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1411	0.1382200470986525	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.795892778076008E-5	0.0	0.0	0.35568886677193984	0.0
2	9.795892778076008E-5	0.0	0.0	0.4655987837419527	0.0
3	9.795892778076008E-5	0.0	0.0	0.5882433613234643	0.0
4	9.795892778076008E-5	0.0	0.0	1.148176592518289	0.0
5	9.795892778076008E-5	0.0	0.0	1.2319314757708388	0.0
6	9.795892778076008E-5	0.0	0.0	1.7587545893757666	0.0
7	9.795892778076008E-5	0.0	0.0	2.2042717929226634	0.0
8	9.795892778076008E-5	0.0	0.0	2.5880748719676814	0.0
9	9.795892778076008E-5	0.0	0.0	3.1542774745404745	0.0
10	1.9591785556152016E-4	0.0	0.0	3.9950589516827386	0.0
11	1.9591785556152016E-4	0.0	0.0	4.841522046636286	0.0
12	1.9591785556152016E-4	0.0	0.0	5.274598466355027	0.0
13	1.9591785556152016E-4	0.0	0.0	5.472671418327724	0.0
14	1.9591785556152016E-4	0.0	0.0	5.658303586472264	0.0
15	1.9591785556152016E-4	0.0	0.0	5.812686856654742	0.0
16	1.9591785556152016E-4	0.0	0.0	6.150743116426145	0.0
17	2.9387678334228025E-4	0.0	0.0	6.6350520553742225	0.0
18	2.9387678334228025E-4	0.0	0.0	7.197140382980224	0.0
19	2.9387678334228025E-4	0.0	0.0	7.45173563628242	0.0
20	2.9387678334228025E-4	0.0	0.0	7.717792084134964	0.0
21	2.9387678334228025E-4	0.0	0.0	8.029497392333342	0.0
22	2.9387678334228025E-4	0.0	0.0	8.387243396588678	0.0
23	2.9387678334228025E-4	0.0	0.0	8.74195267408281	0.0
24	2.9387678334228025E-4	0.0	0.0	8.995960173818322	0.0
25	2.9387678334228025E-4	0.0	0.0	9.219012652375111	0.0
26	2.9387678334228025E-4	0.0	0.0	9.425999866775859	0.0
27	2.9387678334228025E-4	0.0	0.0	9.649640098899333	0.0
28	2.9387678334228025E-4	0.0	0.0	9.88777825233436	0.0
29	2.9387678334228025E-4	0.0	0.0	10.135908216403026	0.0
30	2.9387678334228025E-4	0.0	0.0	10.456233910246112	0.0
31	2.9387678334228025E-4	0.0	0.0	10.684968006614186	0.0
32	2.9387678334228025E-4	0.0	0.0	10.91595515832122	0.0
33	2.9387678334228025E-4	0.0	0.0	11.15575861352852	0.0
34	2.9387678334228025E-4	0.0	0.0	11.395170233024697	0.0
35	2.9387678334228025E-4	0.0	0.0	11.65711240591045	0.0
36	3.9183571112304033E-4	0.0	0.0	11.913862755623821	0.0
37	3.9183571112304033E-4	0.0	0.0	12.16375598039254	0.0
38	3.9183571112304033E-4	0.0	0.0	12.430008346100648	0.0
39	3.9183571112304033E-4	0.0	0.0	12.697926013581025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	20	7.032914E-4	45.000004	18
GATACCC	30	2.1652613E-6	45.000004	27
TCGCAAC	30	2.1652613E-6	45.000004	16
GCGACGA	20	7.032914E-4	45.000004	41
TAGACTA	40	6.8139343E-9	45.000004	41
CGACGAT	20	7.032914E-4	45.000004	16
TCCGAAC	20	7.032914E-4	45.000004	25
CGCATTG	20	7.032914E-4	45.000004	1
TTTCGCA	30	2.1652613E-6	45.000004	14
TAGCGTA	20	7.032914E-4	45.000004	17
TAGAACG	40	6.8139343E-9	45.000004	1
ATTACGC	20	7.032914E-4	45.000004	29
CGCGCGT	20	7.032914E-4	45.000004	22
CGTCTAG	20	7.032914E-4	45.000004	1
TCGATAC	20	7.032914E-4	45.000004	31
ACGATAG	60	0.0	45.000004	1
ATATCCC	20	7.032914E-4	45.000004	15
TACGCCC	20	7.032914E-4	45.000004	31
TCGTGCG	20	7.032914E-4	45.000004	45
GCCGAAC	40	6.8139343E-9	45.000004	23
>>END_MODULE