##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553392_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1093587
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50653400232446	31.0	31.0	34.0	30.0	34.0
2	31.705276306320393	31.0	31.0	34.0	30.0	34.0
3	31.820535540382245	31.0	31.0	34.0	30.0	34.0
4	35.512450312595156	37.0	35.0	37.0	33.0	37.0
5	35.24539428504545	37.0	35.0	37.0	33.0	37.0
6	33.98820761402614	37.0	35.0	37.0	30.0	37.0
7	34.63455125198087	37.0	35.0	37.0	31.0	37.0
8	35.31785125463269	37.0	35.0	37.0	32.0	37.0
9	37.052878280374586	39.0	37.0	39.0	34.0	39.0
10	36.49072273170768	38.0	35.0	39.0	32.0	39.0
11	36.48958336190902	38.0	35.0	39.0	32.0	39.0
12	36.278393945794896	38.0	35.0	39.0	32.0	39.0
13	36.35125234663543	38.0	35.0	39.0	32.0	39.0
14	37.45049456513291	39.0	36.0	41.0	32.0	41.0
15	37.52410919295858	39.0	36.0	41.0	32.0	41.0
16	37.249628973277844	39.0	36.0	41.0	31.0	41.0
17	37.2717945622982	39.0	36.0	40.0	32.0	41.0
18	37.085121714138886	39.0	36.0	40.0	31.0	41.0
19	36.989068085118056	39.0	35.0	40.0	31.0	41.0
20	37.083385226781225	39.0	35.0	40.0	31.0	41.0
21	37.11240806629925	39.0	35.0	40.0	31.0	41.0
22	37.00799113376439	39.0	35.0	40.0	31.0	41.0
23	36.92125729365839	39.0	35.0	40.0	31.0	41.0
24	36.82527224628676	39.0	35.0	40.0	31.0	41.0
25	36.8371944801831	39.0	35.0	40.0	31.0	41.0
26	36.68565555369623	39.0	35.0	40.0	30.0	41.0
27	36.5836947586246	39.0	35.0	40.0	30.0	41.0
28	36.48625669471199	39.0	35.0	40.0	30.0	41.0
29	36.3338060895018	38.0	35.0	40.0	30.0	41.0
30	36.35363167265156	38.0	35.0	40.0	30.0	41.0
31	36.18142223709682	38.0	35.0	40.0	30.0	41.0
32	36.13386863596586	38.0	35.0	40.0	30.0	41.0
33	36.05763510356286	38.0	35.0	40.0	30.0	41.0
34	35.9880485046	38.0	35.0	40.0	29.0	41.0
35	35.73398915678405	38.0	35.0	40.0	28.0	41.0
36	35.75220444281068	38.0	35.0	40.0	28.0	41.0
37	35.60921719076763	38.0	35.0	40.0	27.0	41.0
38	35.615906187619274	38.0	35.0	40.0	28.0	41.0
39	35.61127189697756	38.0	35.0	40.0	28.0	41.0
40	35.45616672473246	38.0	34.0	40.0	27.0	41.0
41	35.38000177397866	38.0	34.0	40.0	27.0	41.0
42	35.307820959832185	38.0	34.0	40.0	27.0	41.0
43	35.33223054041425	38.0	34.0	40.0	27.0	41.0
44	35.16227424064112	38.0	34.0	40.0	26.0	41.0
45	34.98265432928519	38.0	34.0	40.0	26.0	41.0
46	34.72652655892947	37.0	33.0	40.0	25.0	41.0
47	34.68835309856463	37.0	33.0	40.0	25.0	41.0
48	34.61945048724976	37.0	33.0	40.0	24.0	41.0
49	34.39488399185433	37.0	33.0	40.0	24.0	41.0
50	34.29916961339153	37.0	33.0	40.0	24.0	41.0
51	32.90486445065642	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	8.0
11	15.0
12	16.0
13	19.0
14	47.0
15	58.0
16	145.0
17	306.0
18	669.0
19	1495.0
20	3128.0
21	5290.0
22	7051.0
23	8009.0
24	8541.0
25	9254.0
26	10555.0
27	12500.0
28	15598.0
29	19995.0
30	26444.0
31	34459.0
32	44512.0
33	57946.0
34	80597.0
35	96529.0
36	110798.0
37	144184.0
38	197837.0
39	197546.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.49501960063534	39.219193351786366	17.880973347342277	23.404813700236012
2	20.29321855508524	38.28684869150785	19.15613481140504	22.26379794200187
3	19.758464575749347	28.20735798797901	28.768813089402123	23.26536434686952
4	26.606022200337055	28.604948668921633	18.171119444543507	26.617909686197805
5	16.95676704276843	38.194949281584364	17.62950730028795	27.218776375359255
6	19.29192647681437	35.82120123959045	25.028095615620888	19.85877666797429
7	76.04351551362626	13.120583913305481	6.361450895081965	4.474449677986296
8	77.34574386857196	5.466963305159992	4.935226918388752	12.2520659078793
9	76.13258021538294	9.02516214987925	8.320417122734634	6.52184051200316
10	37.63001937660195	32.889838668528434	13.874524843473814	15.6056171113958
11	25.089453331102145	26.476905815449527	25.74911735417484	22.684523499273492
12	27.440980918756345	23.43105761132859	31.540334696736522	17.587626773178542
13	24.2942719692169	28.252164665454142	24.079748570529823	23.373814794799134
14	18.757721150672054	25.73951592328731	31.511073193079287	23.991689732961348
15	18.87787620006456	29.035824310274354	26.069347934823657	26.01695155483743
16	20.633109208503758	29.59499335672425	28.149932287051694	21.621965147720303
17	20.117649533141854	28.42041831148322	26.623121891536748	24.838810263838177
18	20.118198186335427	28.68496059298437	25.58900206385043	25.60783915682977
19	23.478881881368377	29.286284493140464	27.232218378601793	20.002615246889366
20	22.742955064389026	31.786131327457255	25.817058907978975	19.653854700174747
21	22.090149206235992	26.99108530002643	28.883847375654614	22.03491811808297
22	23.088332249743278	30.045437628647743	25.846869064829775	21.019361056779204
23	22.803947011074566	28.199951169865773	24.26866815351682	24.72743366554284
24	19.183110260089045	33.62165058655599	25.468298361264353	21.726940792090616
25	21.225563215363753	27.865089837388336	27.147451460194755	23.761895487053156
26	24.854629764252866	29.763521329350112	22.009131417985035	23.372717488411986
27	19.302899540685836	28.832822628652316	28.69346471748476	23.170813113177093
28	24.326185296643065	26.98203252233247	27.027022084205466	21.664760096819
29	22.86987683650226	30.66395266220246	25.662978802783865	20.803191698511412
30	22.545531356901645	27.249683838597207	25.882531522412027	24.322253282089125
31	25.761462051030232	30.873355297749516	23.4710178522605	19.894164798959753
32	24.0524073530501	25.626676249809115	26.53286844119398	23.788047955946805
33	24.67293411498125	27.867010123565844	24.10800420999884	23.352051551454068
34	23.208030088141136	26.493456853455648	27.097524019579605	23.200989038823614
35	25.401271229449506	26.704414006384496	26.99995519332252	20.89435957084347
36	23.581205701969758	29.286375935339393	26.933110945905536	20.199307416785313
37	21.730232711252054	28.02739974048704	27.81808854713891	22.424279001121995
38	22.302295107750915	29.0790764703677	26.374490552649217	22.24413786923217
39	21.76232892307608	25.67075138969282	30.20747320514966	22.359446482081445
40	23.228696025099055	27.229566554832857	24.98667229950612	24.555065120561967
41	19.4150076765726	25.465829421893275	32.73164366438153	22.3875192371526
42	24.497365093037864	26.254244061057786	24.74581354752754	24.50257729837681
43	22.2061893566767	25.644050267605596	27.898191913400577	24.251568462317127
44	21.96185580113882	24.63169368326434	26.785797563431167	26.62065295216567
45	25.13087664721691	28.083270924032565	25.377496257728012	21.408356171022515
46	19.461826082424167	27.041835720431934	31.54554690207546	21.95079129506843
47	22.02860860635688	27.621487819441892	26.225439768395198	24.12446380580603
48	20.528225006332374	24.463988690428838	32.30799195674418	22.69979434649461
49	21.949602546482357	26.68886883256659	27.765143513959107	23.596385106991946
50	21.285640740060003	25.51584830470735	27.735333357108306	25.463177598124336
51	20.028767715783015	24.321796071094482	31.247171006970635	24.402265206151867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9308.0
1	7348.0
2	5388.0
3	3501.0
4	1614.0
5	1408.0
6	1202.0
7	1315.0
8	1428.0
9	1555.5
10	1683.0
11	2078.5
12	2474.0
13	2671.5
14	2869.0
15	3266.5
16	3664.0
17	3469.0
18	3274.0
19	3672.0
20	4070.0
21	3834.5
22	3599.0
23	4674.5
24	5750.0
25	7298.5
26	10331.0
27	11815.0
28	13365.5
29	14916.0
30	18343.0
31	21770.0
32	24279.0
33	26788.0
34	30213.0
35	33638.0
36	37749.0
37	41860.0
38	41159.0
39	40458.0
40	43610.5
41	46763.0
42	48804.0
43	50845.0
44	79054.5
45	107264.0
46	103385.0
47	99506.0
48	90543.0
49	81580.0
50	77523.0
51	73466.0
52	68576.5
53	63687.0
54	60875.0
55	58063.0
56	55716.5
57	53370.0
58	50838.0
59	48306.0
60	44535.0
61	40764.0
62	37069.5
63	33375.0
64	29847.0
65	26319.0
66	23006.0
67	19693.0
68	16755.5
69	13818.0
70	12742.5
71	11667.0
72	9916.0
73	8165.0
74	6290.5
75	3541.0
76	2666.0
77	2142.0
78	1618.0
79	1211.0
80	804.0
81	700.5
82	597.0
83	388.0
84	179.0
85	121.5
86	64.0
87	58.0
88	52.0
89	63.5
90	75.0
91	58.0
92	41.0
93	23.0
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1093587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.69210373602572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48444460994534	17.58285055017219
2	9.015023163244775	4.091396816060504
3	3.266014989259494	2.2233825281907413
4	1.6900055705926214	1.5339912688939643
5	1.0240777385398219	1.161923913835013
6	0.694894779449111	0.9461174652529167
7	0.5097291340377652	0.8096778470801671
8	0.3941389887495903	0.7155074255294384
9	0.3249778566410674	0.6636988111329356
>10	3.7360554716401824	19.83488105693794
>50	1.2197789081583532	19.76487335928461
>100	0.6288839798820312	21.298856533102043
>500	0.005780942690970739	0.8933477259139436
>1k	0.0037163203013383323	1.8193971883858868
>5k	0.001238773433779444	1.7143580861465821
>10k+	0.001238773433779444	4.945739424081159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT	18179	1.662327734327493	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	17422	1.5931059897383564	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	17181	1.5710684197965046	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7975	0.7292515364575475	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	5184	0.4740363592471381	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	5137	0.4697385758974823	TruSeq Adapter, Index 16 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	3788	0.34638304954246896	No Hit
AACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	3092	0.28273927908799207	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTAT	2694	0.24634528391431135	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATCTGTC	2216	0.20263591282632293	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1792	0.16386442048049218	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1675	0.1531656832058172	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	1629	0.14895934205509026	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1507	0.13780339378577103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8288439785769215E-4	0.0	0.0	0.5834926713649669	0.0
2	1.8288439785769215E-4	0.0	0.0	0.6846277433802707	0.0
3	1.8288439785769215E-4	0.0	0.0	1.1338832667176915	0.0
4	1.8288439785769215E-4	0.0	0.0	3.36708464895797	0.0
5	1.8288439785769215E-4	0.0	0.0	3.6265061673191066	0.0
6	1.8288439785769215E-4	0.0	0.0	4.554918813043681	0.0
7	1.8288439785769215E-4	0.0	0.0	5.241649726999315	0.0
8	1.8288439785769215E-4	0.0	0.0	5.480130981805746	0.0
9	1.8288439785769215E-4	0.0	0.0	7.742410983305398	0.0
10	1.8288439785769215E-4	0.0	0.0	8.339894311106478	0.0
11	1.8288439785769215E-4	0.0	0.0	10.7203176336222	0.0
12	1.8288439785769215E-4	0.0	0.0	11.097791030800476	0.0
13	1.8288439785769215E-4	0.0	0.0	11.351360248430167	0.0
14	1.8288439785769215E-4	0.0	0.0	11.790374245487556	0.0
15	1.8288439785769215E-4	0.0	0.0	12.00242870480355	0.0
16	1.8288439785769215E-4	0.0	0.0	12.300530273311589	0.0
17	1.8288439785769215E-4	0.0	0.0	12.628807767466146	0.0
18	1.8288439785769215E-4	0.0	0.0	13.388143787371284	0.0
19	1.8288439785769215E-4	0.0	0.0	13.612817270139459	0.0
20	3.657687957153843E-4	0.0	0.0	13.866020718973433	0.0
21	3.657687957153843E-4	0.0	0.0	14.124527815345282	0.0
22	3.657687957153843E-4	0.0	0.0	14.39565393516931	0.0
23	3.657687957153843E-4	0.0	0.0	14.634683843169313	0.0
24	3.657687957153843E-4	0.0	0.0	14.822597561968092	0.0
25	3.657687957153843E-4	0.0	0.0	14.990668323599312	0.0
26	3.657687957153843E-4	0.0	0.0	15.149503423138716	0.0
27	3.657687957153843E-4	0.0	0.0	15.339611754711788	0.0
28	3.657687957153843E-4	0.0	0.0	15.510791551106587	0.0
29	3.657687957153843E-4	0.0	0.0	15.697973732313935	0.0
30	3.657687957153843E-4	0.0	0.0	15.949531221567192	0.0
31	3.657687957153843E-4	0.0	0.0	16.11056093388089	0.0
32	3.657687957153843E-4	0.0	0.0	16.28567274482963	0.0
33	3.657687957153843E-4	0.0	0.0	16.455755234837284	0.0
34	3.657687957153843E-4	0.0	0.0	16.63123281458174	0.0
35	3.657687957153843E-4	0.0	0.0	16.81987807097195	0.0
36	3.657687957153843E-4	0.0	0.0	16.994441228727116	0.0
37	3.657687957153843E-4	0.0	0.0	17.178057164176238	0.0
38	3.657687957153843E-4	0.0	0.0	17.36725107376002	0.0
39	3.657687957153843E-4	0.0	0.0	17.558731038317024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.033137E-4	45.0	20
TAATACG	35	1.2118471E-7	45.0	1
CTCGTCG	40	6.8139343E-9	45.0	30
AAGCGTA	20	7.033137E-4	45.0	22
CGTTCGA	20	7.033137E-4	45.0	44
CCGCTAA	20	7.033137E-4	45.0	35
TCTAGCG	40	6.8139343E-9	45.0	1
ATCGTAG	25	3.8906815E-5	45.0	1
ATATCGT	40	6.8139343E-9	45.0	13
CGTAGCG	25	3.8906815E-5	45.0	1
AACGAAT	20	7.033137E-4	45.0	24
CGTACAT	25	3.8906815E-5	45.0	35
TTAGTCG	35	1.2118471E-7	45.0	1
TTACGTA	20	7.033137E-4	45.0	24
CATAACG	35	1.2118471E-7	45.0	1
TATAGCG	20	7.033137E-4	45.0	1
TACTACG	20	7.033137E-4	45.0	1
ATCGGCC	25	3.8906815E-5	45.0	4
GATTCGA	25	3.8906815E-5	45.0	9
CGTTTAG	35	1.2118471E-7	45.0	1
>>END_MODULE