##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1201155
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.655939491572695	31.0	31.0	34.0	30.0	34.0
2	31.869230032760136	33.0	31.0	34.0	30.0	34.0
3	32.042390865458664	33.0	31.0	34.0	30.0	34.0
4	35.75497750082213	37.0	35.0	37.0	35.0	37.0
5	35.49794239710945	37.0	35.0	37.0	33.0	37.0
6	34.11268404160995	37.0	35.0	37.0	32.0	37.0
7	34.94930129750116	37.0	35.0	37.0	32.0	37.0
8	35.665911560123384	37.0	35.0	37.0	33.0	37.0
9	37.33966473935504	39.0	37.0	39.0	34.0	39.0
10	36.705395223763794	39.0	37.0	39.0	32.0	39.0
11	36.77321244968385	39.0	37.0	39.0	32.0	39.0
12	36.75396763948034	39.0	37.0	39.0	32.0	39.0
13	36.71259745827974	39.0	35.0	39.0	32.0	39.0
14	37.90458600263913	40.0	37.0	41.0	33.0	41.0
15	37.93699314409881	40.0	37.0	41.0	33.0	41.0
16	37.80605667045469	40.0	37.0	41.0	33.0	41.0
17	37.80148523712593	39.0	37.0	41.0	33.0	41.0
18	37.7197181046576	39.0	37.0	41.0	33.0	41.0
19	37.71542223942789	39.0	36.0	41.0	33.0	41.0
20	37.60916784261815	39.0	36.0	41.0	32.0	41.0
21	37.57568007459487	39.0	36.0	41.0	32.0	41.0
22	37.529505351099566	39.0	36.0	41.0	32.0	41.0
23	37.3828856392389	39.0	36.0	41.0	32.0	41.0
24	37.23144556697512	39.0	36.0	41.0	31.0	41.0
25	37.21664897536122	39.0	36.0	41.0	32.0	41.0
26	37.19859884860821	39.0	36.0	41.0	32.0	41.0
27	37.13522984127777	39.0	36.0	41.0	31.0	41.0
28	37.028555015797295	39.0	35.0	41.0	31.0	41.0
29	36.865153955984034	39.0	35.0	40.0	31.0	41.0
30	36.91797311754103	39.0	35.0	40.0	31.0	41.0
31	36.837756992228314	39.0	35.0	40.0	31.0	41.0
32	36.77076064288123	39.0	35.0	40.0	31.0	41.0
33	36.74133479858969	39.0	35.0	40.0	31.0	41.0
34	36.69234361926645	39.0	35.0	40.0	30.0	41.0
35	36.556425274007104	39.0	35.0	40.0	30.0	41.0
36	36.48305089684512	39.0	35.0	40.0	30.0	41.0
37	36.440494357514225	39.0	35.0	40.0	30.0	41.0
38	36.377456697928245	39.0	35.0	40.0	30.0	41.0
39	36.312922145768034	39.0	35.0	40.0	30.0	41.0
40	36.16815898031478	38.0	35.0	40.0	30.0	41.0
41	36.06404002813958	38.0	35.0	40.0	29.0	41.0
42	35.979831911784906	38.0	35.0	40.0	29.0	41.0
43	35.93428158730555	38.0	35.0	40.0	29.0	41.0
44	35.80349413689324	38.0	35.0	40.0	28.0	41.0
45	35.66735350558421	38.0	34.0	40.0	28.0	41.0
46	35.52465751713975	38.0	34.0	40.0	28.0	41.0
47	35.39725763952196	38.0	34.0	40.0	27.0	41.0
48	35.25668127760364	38.0	34.0	40.0	27.0	41.0
49	35.13017720444073	38.0	34.0	40.0	26.0	41.0
50	34.993716048303504	37.0	34.0	40.0	26.0	41.0
51	33.82725293571604	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	5.0
10	9.0
11	10.0
12	20.0
13	11.0
14	28.0
15	71.0
16	162.0
17	251.0
18	534.0
19	1005.0
20	1711.0
21	2854.0
22	3827.0
23	5023.0
24	6469.0
25	8114.0
26	9681.0
27	11960.0
28	14880.0
29	19061.0
30	24609.0
31	31596.0
32	40825.0
33	54366.0
34	77942.0
35	102467.0
36	119366.0
37	165586.0
38	242561.0
39	256105.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.29937851484613	33.29961578647219	21.80085001519371	24.600155683487976
2	23.354271513668095	30.92023926970291	20.93609900470797	24.789390211921024
3	20.871661026262224	31.856421527613005	20.25691938176172	27.01499806436305
4	20.023727162605994	32.49239273865571	20.134786934242456	27.34909316449584
5	18.20322939171048	34.989988802444316	17.353963476820226	29.452818329024982
6	19.03384658932444	42.09548309751864	17.40616323455341	21.46450707860351
7	85.54532928722772	7.37148827586781	3.6996890492900585	3.383493387614421
8	87.14170943799925	6.442299286936323	3.325049639721768	3.0909416353426495
9	79.96561642752185	10.452023260944674	5.888915252402895	3.6934450591305867
10	32.65656805324875	42.66501825326456	11.998617996844704	12.679795696641982
11	23.735404673002236	30.808097206438802	27.926787134050144	17.529710986508817
12	26.010881193517903	25.767199070894264	30.215917179714523	18.006002555873305
13	25.19191944420162	27.76111326181883	27.27083515449713	19.776132139482414
14	19.817342474534925	30.317985605521354	27.0990837985106	22.76558812143312
15	20.299628274452505	30.798190075385772	27.807651801807427	21.09452984835429
16	21.636175181387916	31.936011588845737	26.981363770704032	19.446449459062322
17	22.084160662029465	30.39724265394558	26.69031057607053	20.828286107954426
18	23.090775128938397	29.373561280600757	26.283285670875117	21.252377919585733
19	22.458550311991374	31.39220167255683	26.669580528741086	19.47966748671071
20	23.667303553662933	29.524332829651463	27.126057835999518	19.68230578068609
21	25.344855576507612	29.432254788099787	27.157361039999	18.0655285953936
22	23.761879191278396	28.67165353347403	26.478597683063388	21.087869592184187
23	22.02080497521136	29.240855676411453	26.77789294470739	21.9604464036698
24	21.200094908650424	29.266164649857846	27.983732324304523	21.550008117187208
25	21.612614525186174	31.526822100395037	25.436517352048654	21.424046022370135
26	23.82540138450075	30.542019972443192	24.170569160516337	21.462009482539724
27	20.746531463466415	29.894393313102807	26.553442311774916	22.805632911655866
28	21.126915343981416	30.71019144073829	27.374402137942234	20.788491077338062
29	21.29300548222336	30.47883079202934	26.36370826412911	21.864455461618192
30	22.307029484121532	29.51450895180056	26.650182532645662	21.528279031432245
31	22.77033355395432	30.83099183702353	24.13368799197439	22.264986617047757
32	22.080997040348667	29.916372158464142	25.845457080892974	22.157173720294217
33	21.51337670825164	29.369065607685936	26.321915156661714	22.795642527400712
34	20.507511520161845	29.731799809350168	27.037975948149906	22.722712722338084
35	21.574234799005957	28.672902331505924	28.021029758857104	21.73183311063102
36	21.693952903663558	30.93980377220259	25.93045860026391	21.43578472386994
37	20.554216566554693	31.635134516361337	25.584708051833445	22.22594086525053
38	21.93538718982979	29.793490432125747	26.43439023273433	21.83673214531014
39	22.212786859314576	29.062111051446315	26.129766766154244	22.595335323084864
40	23.159042754681952	29.995129687675615	25.59594723412049	21.249880323521943
41	20.118469306625705	28.50806099129588	28.92058060783163	22.452889094246785
42	21.67930034008933	28.766645437100124	27.63898081429957	21.915073408510974
43	21.61452934883508	28.623200169836533	27.301222573273225	22.46104790805516
44	21.980843438190742	28.393338078765858	26.354883424703722	23.27093505833968
45	21.674971173578765	28.578160187486212	26.93557450953457	22.81129412940045
46	20.713729701828658	29.075514816988644	27.799659494403308	22.41109598677939
47	21.39673897207271	29.608501817001137	27.163105510945712	21.831653699980436
48	21.18244522979965	28.33847421856463	28.569002335252318	21.910078216383397
49	21.837314917725024	28.30483992490561	27.621830654661554	22.23601450270781
50	21.169873996278582	28.354375580170753	27.47580453813205	22.99994588541862
51	21.107101081875363	26.794710091536896	28.907842867906307	23.190345958681434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15944.0
1	12198.0
2	8452.0
3	5355.0
4	2258.0
5	1990.0
6	1722.0
7	1826.0
8	1930.0
9	2009.5
10	2089.0
11	2315.0
12	2541.0
13	2906.0
14	3271.0
15	3035.5
16	2800.0
17	2940.0
18	3080.0
19	3275.5
20	3471.0
21	4156.5
22	4842.0
23	6625.0
24	8408.0
25	11215.5
26	13999.5
27	13976.0
28	19738.0
29	25500.0
30	26534.0
31	27568.0
32	34870.5
33	42173.0
34	44645.0
35	47117.0
36	46472.5
37	45828.0
38	50317.5
39	54807.0
40	55231.0
41	55655.0
42	58288.0
43	60921.0
44	66152.5
45	71384.0
46	78374.5
47	85365.0
48	87692.5
49	90020.0
50	89028.5
51	88037.0
52	83192.5
53	78348.0
54	72598.5
55	66849.0
56	61826.5
57	56804.0
58	51114.5
59	45425.0
60	44315.0
61	43205.0
62	40495.0
63	37785.0
64	32805.5
65	27826.0
66	23860.5
67	19895.0
68	17299.5
69	14704.0
70	12405.0
71	10106.0
72	8227.5
73	6349.0
74	5274.0
75	3708.5
76	3218.0
77	2403.0
78	1588.0
79	1309.0
80	1030.0
81	720.0
82	410.0
83	259.0
84	108.0
85	79.0
86	50.0
87	44.5
88	39.0
89	21.5
90	4.0
91	6.0
92	8.0
93	5.5
94	3.0
95	3.5
96	4.0
97	3.0
98	2.0
99	8.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1201155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.703596100545017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67212064925111	16.080922141948975
2	9.22590970288316	3.820190162971844
3	3.349182697053066	2.0802037748016207
4	1.7049422376705619	1.4119374185396294
5	1.0148162456270364	1.0505172832866823
6	0.6779324923096858	0.8421384302529344
7	0.49409364058962557	0.7160660619430823
8	0.39095353689320644	0.6475315297533176
9	0.2735833346433783	0.5097742974267074
>10	2.9639704360729784	14.435112697724554
>50	1.1176904928898488	16.741615140990078
>100	1.106146658666513	38.39184038859628
>500	0.0045350776165977045	0.6139710390505002
>1k	0.003710518049943576	1.492035835201271
>5k	0.0	0.0
>10k+	4.1227978332706406E-4	1.1661437975125724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13662	1.13740524744933	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3309	0.27548484583588295	No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	2911	0.2423500713896208	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2521	0.209881322560369	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2164	0.1801599294012846	No Hit
ACCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTC	1661	0.13828356873176234	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1583	0.131789818965912	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGT	1227	0.10215167900895387	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.497596063788603E-4	0.0	0.0	0.3059555178141039	0.0
2	2.497596063788603E-4	0.0	0.0	0.39395415246158905	0.0
3	2.497596063788603E-4	0.0	0.0	0.5116741802681586	0.0
4	2.497596063788603E-4	0.0	0.0	1.0620610995250406	0.0
5	2.497596063788603E-4	0.0	0.0	1.1387372986833506	0.0
6	2.497596063788603E-4	0.0	0.0	1.5982117212183273	0.0
7	2.497596063788603E-4	0.0	0.0	1.9474589041381005	0.0
8	2.497596063788603E-4	0.0	0.0	2.271313860409356	0.0
9	2.497596063788603E-4	0.0	0.0	2.8511724132189435	0.0
10	2.497596063788603E-4	0.0	0.0	3.667303553662933	0.0
11	2.497596063788603E-4	0.0	0.0	4.419995754086692	0.0
12	2.497596063788603E-4	0.0	0.0	4.748179876868514	0.0
13	2.497596063788603E-4	0.0	0.0	4.902198300802144	0.0
14	2.497596063788603E-4	0.0	0.0	5.061794689278236	0.0
15	2.497596063788603E-4	0.0	0.0	5.195166319084548	0.0
16	2.497596063788603E-4	0.0	0.0	5.526680569951422	0.0
17	2.497596063788603E-4	0.0	0.0	5.853782401105603	0.0
18	3.3301280850514715E-4	0.0	0.0	6.4797632278931525	0.0
19	3.3301280850514715E-4	0.0	0.0	6.661754727741216	0.0
20	3.3301280850514715E-4	0.0	0.0	6.9911043953528065	0.0
21	3.3301280850514715E-4	0.0	0.0	7.223963601700031	0.0
22	3.3301280850514715E-4	0.0	0.0	7.4921221657488	0.0
23	3.3301280850514715E-4	0.0	0.0	7.769355328829335	0.0
24	3.3301280850514715E-4	0.0	0.0	7.949348751826367	0.0
25	3.3301280850514715E-4	0.0	0.0	8.119435043770372	0.0
26	3.3301280850514715E-4	0.0	0.0	8.274952025342275	0.0
27	3.3301280850514715E-4	0.0	0.0	8.470264037530544	0.0
28	3.3301280850514715E-4	0.0	0.0	8.646677572836145	0.0
29	4.162660106314339E-4	0.0	0.0	8.830916909141617	0.0
30	4.162660106314339E-4	0.0	0.0	9.094413293871316	0.0
31	4.162660106314339E-4	0.0	0.0	9.278236364166156	0.0
32	4.162660106314339E-4	0.0	0.0	9.474880427588445	0.0
33	4.162660106314339E-4	0.0	0.0	9.665863273266147	0.0
34	4.162660106314339E-4	0.0	0.0	9.860675766241659	0.0
35	4.162660106314339E-4	0.0	0.0	10.063980085834052	0.0
36	4.162660106314339E-4	0.0	0.0	10.243473989618325	0.0
37	4.162660106314339E-4	0.0	0.0	10.442782155508656	0.0
38	4.162660106314339E-4	0.0	0.0	10.63617934404802	0.0
39	4.162660106314339E-4	0.0	0.0	10.856800329682681	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	30	2.1655833E-6	45.000004	1
CGAAACG	45	3.8562575E-10	45.000004	1
CCTACGC	30	2.1655833E-6	45.000004	19
CGACATT	30	2.1655833E-6	45.000004	32
TATGCGA	45	3.8562575E-10	45.000004	45
CGTAGCA	30	2.1655833E-6	45.000004	16
CGTAGAA	30	2.1655833E-6	45.000004	21
TCTAACG	45	3.8562575E-10	45.000004	1
CCGCACG	45	3.8562575E-10	45.000004	1
CGAAGTT	30	2.1655833E-6	45.000004	25
ACGCGAT	80	0.0	45.0	22
TCACGCG	20	7.0334144E-4	45.0	1
CGAACTA	25	3.8909107E-5	45.0	26
GCGACGT	20	7.0334144E-4	45.0	37
GTCGAGT	20	7.0334144E-4	45.0	13
CGACGTC	20	7.0334144E-4	45.0	38
TTTACGC	25	3.8909107E-5	45.0	33
CGACGCT	25	3.8909107E-5	45.0	18
CGTTGCA	20	7.0334144E-4	45.0	19
TTATGCG	20	7.0334144E-4	45.0	1
>>END_MODULE