##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1193521
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63524479250889	31.0	31.0	34.0	30.0	34.0
2	31.853093493956116	33.0	31.0	34.0	30.0	34.0
3	32.029877982875874	33.0	31.0	34.0	30.0	34.0
4	35.75291511418735	37.0	35.0	37.0	35.0	37.0
5	35.52008720416315	37.0	35.0	37.0	33.0	37.0
6	32.658357079598936	37.0	35.0	37.0	26.0	37.0
7	34.22433203940274	37.0	35.0	37.0	27.0	37.0
8	35.37422969516246	37.0	35.0	37.0	32.0	37.0
9	37.21765012932324	39.0	37.0	39.0	34.0	39.0
10	36.664811930414295	38.0	37.0	39.0	32.0	39.0
11	36.76631496220008	39.0	37.0	39.0	32.0	39.0
12	36.766453208615516	39.0	37.0	39.0	32.0	39.0
13	36.742350574476696	39.0	37.0	39.0	32.0	39.0
14	37.908903152939914	40.0	37.0	41.0	33.0	41.0
15	37.96883842010321	40.0	37.0	41.0	33.0	41.0
16	37.871966224306064	40.0	37.0	41.0	33.0	41.0
17	37.88851809059078	40.0	37.0	41.0	33.0	41.0
18	37.795264599449865	39.0	37.0	41.0	33.0	41.0
19	37.7909898527131	40.0	37.0	41.0	33.0	41.0
20	37.668540394345804	39.0	36.0	41.0	33.0	41.0
21	37.61949643114784	39.0	36.0	41.0	32.0	41.0
22	37.541927624231164	39.0	36.0	41.0	32.0	41.0
23	37.41522687912487	39.0	36.0	41.0	32.0	41.0
24	37.30755973292468	39.0	36.0	41.0	32.0	41.0
25	37.276641131576234	39.0	36.0	41.0	32.0	41.0
26	37.26213196081175	39.0	36.0	41.0	32.0	41.0
27	37.17306188998769	39.0	36.0	41.0	31.0	41.0
28	37.087041618873904	39.0	36.0	41.0	31.0	41.0
29	36.90354505702036	39.0	35.0	40.0	31.0	41.0
30	36.96486111262391	39.0	35.0	40.0	31.0	41.0
31	36.589861426820306	39.0	35.0	40.0	30.0	41.0
32	36.70444340736359	39.0	35.0	40.0	30.0	41.0
33	36.713375801515014	39.0	35.0	40.0	30.0	41.0
34	36.70761302063391	39.0	35.0	40.0	31.0	41.0
35	36.64724625708303	39.0	35.0	40.0	30.0	41.0
36	36.54799454722623	39.0	35.0	40.0	30.0	41.0
37	36.4905870948228	39.0	35.0	40.0	30.0	41.0
38	36.42199341276777	39.0	35.0	40.0	30.0	41.0
39	36.34105809617091	39.0	35.0	40.0	30.0	41.0
40	36.21577249164447	39.0	35.0	40.0	30.0	41.0
41	36.183091876891986	38.0	35.0	40.0	30.0	41.0
42	36.07807487258289	38.0	35.0	40.0	30.0	41.0
43	36.036444268680654	38.0	35.0	40.0	29.0	41.0
44	35.86810537895856	38.0	35.0	40.0	29.0	41.0
45	35.72114943934795	38.0	34.0	40.0	28.0	41.0
46	35.490066785586514	38.0	34.0	40.0	27.0	41.0
47	35.43185499040235	38.0	34.0	40.0	27.0	41.0
48	35.385384924102716	38.0	34.0	40.0	27.0	41.0
49	35.3029297347931	38.0	34.0	40.0	27.0	41.0
50	35.1358819828055	38.0	34.0	40.0	27.0	41.0
51	34.03672411293978	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	10.0
11	5.0
12	16.0
13	27.0
14	50.0
15	69.0
16	134.0
17	258.0
18	521.0
19	943.0
20	1733.0
21	2808.0
22	3895.0
23	4871.0
24	6229.0
25	7610.0
26	9648.0
27	11562.0
28	14536.0
29	18777.0
30	23995.0
31	31855.0
32	40869.0
33	54528.0
34	78576.0
35	103184.0
36	122327.0
37	169374.0
38	238088.0
39	246987.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.290971000929183	33.19665091774674	21.965847270387368	24.546530810936716
2	23.48069284076275	30.754130006929074	20.97818136421563	24.786995788092543
3	20.998792647971843	31.804132478607418	20.2630703607226	26.934004512698145
4	19.938233177296418	32.55912547831165	20.220507221908957	27.282134122482972
5	18.275924763787145	34.95154253674632	17.403547989520085	29.368984709946456
6	18.263021765012933	44.506380700465265	16.603980994050378	20.626616540471428
7	85.63569472175186	7.346665873495313	3.67710329353233	3.3405361112204983
8	87.07689265626662	6.525901094325111	3.27116154638251	3.126044703025753
9	80.05439368054688	10.476564719012066	5.836344731261536	3.6326968691795116
10	32.73423760453314	42.59891531024591	12.000542931376993	12.666304153843964
11	23.849685091422774	30.788649717935417	27.91748113355358	17.44418405708823
12	26.060538524248837	25.921789394572865	30.106382711322215	17.911289369856082
13	25.168555894701477	27.78535107467736	27.283558479490516	19.762534551130646
14	19.879583182868167	30.313500977360263	27.04803685900793	22.75887898076364
15	20.317782426953528	30.865983924874385	27.755272006106303	21.060961642065788
16	21.62643137406045	31.959219821016976	26.97991907976483	19.43442972515775
17	22.130318611905448	30.491964531834796	26.59852654456855	20.779190311691206
18	23.106254519191534	29.428891489969594	26.26748921887424	21.197364771964633
19	22.512130075633358	31.261620030146098	26.61620532860335	19.610044565617198
20	23.729787745670166	29.47882777093993	27.14430663557658	19.647077847813318
21	25.346768092057033	29.413977634243555	27.137101064832542	18.102153208866874
22	23.747215172585985	28.623459495057062	26.566185261926684	21.063140070430265
23	22.05935211864726	29.268022933823534	26.77020345683067	21.90242149069853
24	21.130922706848057	29.375938923571514	28.092593259775068	21.400545109805357
25	21.580851949819067	31.55654571641387	25.38028237458746	21.482319959179605
26	23.729368817138532	30.699920654936108	24.16949513246939	21.401215395455967
27	20.826696807178088	29.952887297332847	26.52043826627265	22.699977629216413
28	21.11207092292469	30.826353285782155	27.36156297208009	20.700012819213068
29	21.236157553993603	30.379188971119902	26.47309934219842	21.91155413268807
30	22.369526803466382	29.431823989691008	26.764338457387847	21.434310749454767
31	22.843837686978276	30.743991936463622	24.22521262717623	22.18695774938187
32	22.106439685602517	29.973331009676414	25.844790330459205	22.075438974261868
33	21.457770747226064	29.32834864237831	26.402300420352887	22.811580190042736
34	20.502948837934145	29.773418314382404	27.14288227856904	22.58075056911441
35	21.67226215542081	28.64549513582082	28.054470763396704	21.627771945361665
36	21.703933152412063	30.982027128135993	25.871685542189876	21.442354177262068
37	20.57584240243783	31.728138842969667	25.558997286180972	22.137021468411533
38	21.964841841911454	29.953054868745504	26.364177923974523	21.71792536536852
39	22.266637956097966	29.081180808716393	26.024426884822304	22.627754350363336
40	23.182750869067238	29.83064395180311	25.622925780107764	21.363679399021883
41	20.083936520597458	28.595726426263134	28.965807891105396	22.354529162034016
42	21.708206223434694	28.758019339416734	27.707430367794117	21.826344069354455
43	21.612271589691343	28.66853620506049	27.363154900500287	22.356037304747883
44	22.061279189892762	28.44432565493192	26.3914082785305	23.102986876644817
45	21.61235537539767	28.595810211969457	26.924369156470647	22.867465256162227
46	20.65242253801986	29.198061869041265	27.819200500033098	22.33031509290578
47	21.365355113148407	29.84430102193426	27.12352778040772	21.666816084509616
48	21.166782989155617	28.41123030093312	28.60092114005535	21.821065569855914
49	21.89002120616227	28.41039244386986	27.573038094847096	22.126548255120774
50	21.151450204897944	28.461334153316116	27.534329098524452	22.85288654326149
51	21.0696753555237	27.012427933819342	28.8022581923569	23.115638518300056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16007.0
1	12276.5
2	8546.0
3	5365.0
4	2184.0
5	1957.0
6	1730.0
7	1818.5
8	1907.0
9	1991.0
10	2075.0
11	2293.5
12	2512.0
13	2857.5
14	3203.0
15	2885.0
16	2567.0
17	2819.5
18	3072.0
19	3327.0
20	3582.0
21	4224.5
22	4867.0
23	6649.0
24	8431.0
25	11230.0
26	14145.0
27	14261.0
28	20044.5
29	25828.0
30	26709.5
31	27591.0
32	34773.0
33	41955.0
34	44684.0
35	47413.0
36	46851.0
37	46289.0
38	50506.0
39	54723.0
40	55113.0
41	55503.0
42	58347.5
43	61192.0
44	66202.5
45	71213.0
46	77452.5
47	83692.0
48	86521.0
49	89350.0
50	87859.5
51	86369.0
52	81622.5
53	76876.0
54	71419.5
55	65963.0
56	61028.5
57	56094.0
58	50726.5
59	45359.0
60	44246.5
61	43134.0
62	40351.0
63	37568.0
64	32665.5
65	27763.0
66	23494.0
67	19225.0
68	16920.5
69	14616.0
70	12270.5
71	9925.0
72	8087.5
73	6250.0
74	5319.5
75	3773.5
76	3158.0
77	2363.5
78	1569.0
79	1248.5
80	928.0
81	668.5
82	409.0
83	256.5
84	104.0
85	72.0
86	40.0
87	33.0
88	26.0
89	18.0
90	10.0
91	8.0
92	6.0
93	5.5
94	5.0
95	4.0
96	3.0
97	3.5
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1193521.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.88915650199668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57862394768486	15.996628603507402
2	9.160571924791558	3.8271324116953562
3	3.4284520509708734	2.1485241435696625
4	1.8107717750412582	1.5130197999294068
5	1.1586430288052145	1.2101537779329785
6	0.8120571467680817	1.0177913296442007
7	0.5969078848327964	0.8728231556483668
8	0.4910957204654928	0.8206860289811595
9	0.3654152134457102	0.6869894023690166
>10	3.4568763142045085	16.230955263000467
>50	1.0918693329802565	16.519042963349793
>100	1.0413876779130793	36.06523629461792
>500	0.0036639910481695522	0.5548131557273192
>1k	0.0032568809317062683	1.3748719019993205
>5k	0.0	0.0
>10k+	4.0711011646328354E-4	1.1613317680276318
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13656	1.1441776055888417	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3425	0.2869660441667972	No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	2810	0.23543783477626284	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2515	0.21072105141007155	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2089	0.1750283405151648	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1601	0.13414091582804158	No Hit
ACCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTC	1588	0.13305170164580263	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6757141265214437E-4	0.0	0.0	0.287887686936384	0.0
2	1.6757141265214437E-4	0.0	0.0	0.36371375116147936	0.0
3	1.6757141265214437E-4	0.0	0.0	0.4702053839019171	0.0
4	1.6757141265214437E-4	0.0	0.0	0.9653789082890037	0.0
5	1.6757141265214437E-4	0.0	0.0	1.0367643300788172	0.0
6	1.6757141265214437E-4	0.0	0.0	1.470606717435219	0.0
7	1.6757141265214437E-4	0.0	0.0	1.7953601151550749	0.0
8	1.6757141265214437E-4	0.0	0.0	2.10519965714889	0.0
9	1.6757141265214437E-4	0.0	0.0	2.6577663903693356	0.0
10	1.6757141265214437E-4	0.0	0.0	3.443927672826871	0.0
11	1.6757141265214437E-4	0.0	0.0	4.172109246506764	0.0
12	1.6757141265214437E-4	0.0	0.0	4.504152000676989	0.0
13	1.6757141265214437E-4	0.0	0.0	4.6551338434765706	0.0
14	1.6757141265214437E-4	0.0	0.0	4.7988263298257845	0.0
15	1.6757141265214437E-4	0.0	0.0	4.933805102717087	0.0
16	1.6757141265214437E-4	0.0	0.0	5.248001501439857	0.0
17	1.6757141265214437E-4	0.0	0.0	5.572754899159714	0.0
18	1.6757141265214437E-4	0.0	0.0	6.176430913239063	0.0
19	1.6757141265214437E-4	0.0	0.0	6.355229610538902	0.0
20	1.6757141265214437E-4	0.0	0.0	6.66263936704926	0.0
21	1.6757141265214437E-4	0.0	0.0	6.884336345988047	0.0
22	1.6757141265214437E-4	0.0	0.0	7.1378718933307415	0.0
23	1.6757141265214437E-4	0.0	0.0	7.406740224931108	0.0
24	1.6757141265214437E-4	0.0	0.0	7.5889741361903145	0.0
25	1.6757141265214437E-4	0.0	0.0	7.756461763136133	0.0
26	1.6757141265214437E-4	0.0	0.0	7.907192248816736	0.0
27	1.6757141265214437E-4	0.0	0.0	8.103585944445049	0.0
28	1.6757141265214437E-4	0.0	0.0	8.27702235654002	0.0
29	1.6757141265214437E-4	0.0	0.0	8.453558839769054	0.0
30	1.6757141265214437E-4	0.0	0.0	8.714886457800072	0.0
31	1.6757141265214437E-4	0.0	0.0	8.891674298148084	0.0
32	1.6757141265214437E-4	0.0	0.0	9.084381422698051	0.0
33	1.6757141265214437E-4	0.0	0.0	9.27935076131882	0.0
34	1.6757141265214437E-4	0.0	0.0	9.463176600998223	0.0
35	1.6757141265214437E-4	0.0	0.0	9.6645974390061	0.0
36	1.6757141265214437E-4	0.0	0.0	9.850936849875286	0.0
37	1.6757141265214437E-4	0.0	0.0	10.048000831154207	0.0
38	1.6757141265214437E-4	0.0	0.0	10.24481345531415	0.0
39	1.6757141265214437E-4	0.0	0.0	10.461902220405003	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCGT	35	1.2119744E-7	45.000004	20
ATTCGCG	70	0.0	45.000004	1
GTATCGG	85	0.0	45.000004	2
CGCGTAG	35	1.2119744E-7	45.000004	1
TCGTTGC	25	3.8908966E-5	45.0	14
GTCGACT	20	7.033395E-4	45.0	17
ACGTTAC	25	3.8908966E-5	45.0	29
CTATCGA	20	7.033395E-4	45.0	1
CGCATTA	20	7.033395E-4	45.0	38
TTTCGAC	25	3.8908966E-5	45.0	13
ATTACGA	25	3.8908966E-5	45.0	24
ATATCCG	50	2.1827873E-11	45.0	1
CGTAGCG	50	2.1827873E-11	45.0	1
GTATCGC	20	7.033395E-4	45.0	42
CGTGTAT	20	7.033395E-4	45.0	30
TAGATCG	45	3.8562575E-10	45.0	1
ACCGTAA	25	3.8908966E-5	45.0	18
CACGTTA	25	3.8908966E-5	45.0	28
TCGACGC	40	6.8157533E-9	45.0	17
CGTTTAG	55	1.8189894E-12	45.0	29
>>END_MODULE