Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553383_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 426023 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 11201 | 2.6292007708504 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC | 6065 | 1.4236320574241297 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGT | 6012 | 1.4111914145480409 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT | 4448 | 1.044075085147985 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2484 | 0.5830671114000887 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2147 | 0.5039634010370333 | No Hit |
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT | 2145 | 0.503493942815294 | TruSeq Adapter, Index 14 (95% over 22bp) |
AATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT | 1227 | 0.2880126190370003 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1202 | 0.2821443912652603 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT | 1152 | 0.2704079357217803 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT | 872 | 0.20468378467829199 | No Hit |
AACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG | 851 | 0.19975447335003038 | TruSeq Adapter, Index 15 (95% over 22bp) |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 777 | 0.18238451914567994 | No Hit |
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTATC | 730 | 0.1713522509348087 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTCGGT | 30 | 2.1622727E-6 | 45.000004 | 41 |
ATCTCGC | 30 | 2.1622727E-6 | 45.000004 | 29 |
GTCGAGC | 30 | 2.1622727E-6 | 45.000004 | 27 |
ATAGGCG | 30 | 2.1622727E-6 | 45.000004 | 26 |
CAACGAC | 30 | 2.1622727E-6 | 45.000004 | 12 |
CGTTGTC | 30 | 2.1622727E-6 | 45.000004 | 45 |
CTATACG | 30 | 2.1622727E-6 | 45.000004 | 1 |
GACCGAG | 30 | 2.1622727E-6 | 45.000004 | 1 |
CTCCACG | 30 | 2.1622727E-6 | 45.000004 | 1 |
CGTTACT | 30 | 2.1622727E-6 | 45.000004 | 31 |
TGCGAAG | 30 | 2.1622727E-6 | 45.000004 | 1 |
CGGGCAC | 30 | 2.1622727E-6 | 45.000004 | 6 |
GAGCCGA | 30 | 2.1622727E-6 | 45.000004 | 41 |
ACAGACG | 30 | 2.1622727E-6 | 45.000004 | 1 |
TTCGGTG | 30 | 2.1622727E-6 | 45.000004 | 30 |
ACCGTTG | 30 | 2.1622727E-6 | 45.000004 | 43 |
TCGAGCG | 30 | 2.1622727E-6 | 45.000004 | 1 |
TCGGTGA | 30 | 2.1622727E-6 | 45.000004 | 31 |
TCGAACG | 30 | 2.1622727E-6 | 45.000004 | 1 |
TACCGTG | 30 | 2.1622727E-6 | 45.000004 | 27 |