FastQCFastQC Report
Sat 18 Jun 2016
SRR3553383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426023
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112012.6292007708504No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC60651.4236320574241297No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGT60121.4111914145480409No Hit
AATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT44481.044075085147985No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24840.5830671114000887No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21470.5039634010370333No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT21450.503493942815294TruSeq Adapter, Index 14 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT12270.2880126190370003No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12020.2821443912652603No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT11520.2704079357217803No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT8720.20468378467829199No Hit
AACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG8510.19975447335003038TruSeq Adapter, Index 15 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7770.18238451914567994No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTATC7300.1713522509348087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGT302.1622727E-645.00000441
ATCTCGC302.1622727E-645.00000429
GTCGAGC302.1622727E-645.00000427
ATAGGCG302.1622727E-645.00000426
CAACGAC302.1622727E-645.00000412
CGTTGTC302.1622727E-645.00000445
CTATACG302.1622727E-645.0000041
GACCGAG302.1622727E-645.0000041
CTCCACG302.1622727E-645.0000041
CGTTACT302.1622727E-645.00000431
TGCGAAG302.1622727E-645.0000041
CGGGCAC302.1622727E-645.0000046
GAGCCGA302.1622727E-645.00000441
ACAGACG302.1622727E-645.0000041
TTCGGTG302.1622727E-645.00000430
ACCGTTG302.1622727E-645.00000443
TCGAGCG302.1622727E-645.0000041
TCGGTGA302.1622727E-645.00000431
TCGAACG302.1622727E-645.0000041
TACCGTG302.1622727E-645.00000427