##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553383_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426023
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477727728315138	31.0	31.0	34.0	30.0	34.0
2	31.70048565453039	31.0	31.0	34.0	30.0	34.0
3	31.836276445168455	31.0	31.0	34.0	30.0	34.0
4	35.55686899533593	37.0	35.0	37.0	33.0	37.0
5	35.30616656847165	37.0	35.0	37.0	33.0	37.0
6	32.51176814397345	37.0	35.0	37.0	26.0	37.0
7	34.03261326266422	37.0	35.0	37.0	26.0	37.0
8	35.151123296160065	37.0	35.0	37.0	32.0	37.0
9	37.02953126943851	39.0	37.0	39.0	34.0	39.0
10	36.50592808369501	38.0	35.0	39.0	32.0	39.0
11	36.594362276215136	38.0	35.0	39.0	32.0	39.0
12	36.50139311727301	38.0	35.0	39.0	32.0	39.0
13	36.585942543008244	39.0	35.0	39.0	32.0	39.0
14	37.63855472591855	39.0	37.0	41.0	32.0	41.0
15	37.786666447586164	39.0	37.0	41.0	33.0	41.0
16	37.58434169047211	39.0	36.0	41.0	32.0	41.0
17	37.56922748302322	39.0	36.0	41.0	32.0	41.0
18	37.348345042403814	39.0	36.0	40.0	32.0	41.0
19	37.23628301758356	39.0	35.0	41.0	32.0	41.0
20	37.26343178654673	39.0	35.0	40.0	32.0	41.0
21	37.28248709576713	39.0	35.0	40.0	32.0	41.0
22	37.14150644448774	39.0	35.0	40.0	31.0	41.0
23	37.01426214077644	39.0	35.0	40.0	31.0	41.0
24	36.947385469798576	39.0	35.0	40.0	31.0	41.0
25	36.99831933956617	39.0	35.0	40.0	31.0	41.0
26	36.87850186492279	39.0	35.0	40.0	31.0	41.0
27	36.81490670691488	39.0	35.0	40.0	31.0	41.0
28	36.748452548336594	39.0	35.0	40.0	31.0	41.0
29	36.56905378348117	39.0	35.0	40.0	30.0	41.0
30	36.664079169434515	39.0	35.0	40.0	31.0	41.0
31	36.176175934163176	38.0	35.0	40.0	30.0	41.0
32	36.228297533231775	38.0	35.0	40.0	30.0	41.0
33	36.26100468754034	38.0	35.0	40.0	30.0	41.0
34	36.069334284768665	38.0	35.0	40.0	30.0	41.0
35	36.163026409372264	38.0	35.0	40.0	30.0	41.0
36	36.055950030866875	38.0	35.0	40.0	30.0	41.0
37	35.98061137544217	38.0	35.0	40.0	30.0	41.0
38	35.98063954293548	38.0	35.0	40.0	30.0	41.0
39	35.748701361194115	38.0	35.0	40.0	29.0	41.0
40	35.65976719566784	38.0	35.0	40.0	28.0	41.0
41	35.64788990265784	38.0	35.0	40.0	28.0	41.0
42	35.63614171065881	38.0	35.0	40.0	28.0	41.0
43	35.654490485255494	38.0	35.0	40.0	28.0	41.0
44	35.509087537527314	38.0	35.0	40.0	27.0	41.0
45	35.306206472420506	38.0	34.0	40.0	26.0	41.0
46	34.93609969414797	38.0	33.0	40.0	25.0	41.0
47	34.98482006840006	38.0	34.0	40.0	26.0	41.0
48	34.955387385188125	38.0	34.0	40.0	26.0	41.0
49	34.887780706675464	37.0	34.0	40.0	26.0	41.0
50	34.73646493264448	37.0	33.0	40.0	25.0	41.0
51	33.49827826197177	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	1.0
13	9.0
14	5.0
15	20.0
16	39.0
17	96.0
18	199.0
19	455.0
20	956.0
21	1587.0
22	2081.0
23	2507.0
24	2892.0
25	3271.0
26	3611.0
27	4340.0
28	5598.0
29	7291.0
30	9388.0
31	12741.0
32	17054.0
33	22330.0
34	30767.0
35	42449.0
36	44850.0
37	57554.0
38	76710.0
39	77200.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.536431131652517	40.375754360680055	20.507578229344425	22.580236278323003
2	20.265103057816127	38.18127190316016	19.879443128657375	21.674181910366343
3	17.932130424883162	32.70339864279628	26.059391159632227	23.305079772688327
4	22.523666562603427	33.81624935742906	18.425531015931064	25.234553064036447
5	16.85801001354387	41.28251291596933	17.173720667663485	24.685756402823323
6	16.531032362102515	43.12490170718482	21.6683136825946	18.67575224811806
7	77.1108602117726	14.042199599552138	5.072965544113815	3.7739746445614437
8	78.29694640899669	8.737321693899155	3.589008105196198	9.37672379190795
9	74.849245228544	13.063613936336772	7.335519443785898	4.751621391333332
10	30.19156242738068	45.92193379230699	12.409423904343193	11.477079875969137
11	19.648469683561686	28.999373273273974	32.413038732650584	18.939118310513752
12	22.871065646690436	26.848550430375823	34.12656124199867	16.15382268093507
13	22.68070033777519	30.631444781150314	26.55279175068013	20.135063130394368
14	16.326348577424223	31.205592186337356	31.460977458963484	21.007081777274937
15	15.750323339350222	34.241109048103034	26.97647779579976	23.032089816746986
16	17.873448147165764	34.063184382063874	29.483854158108834	18.579513312661522
17	17.26409137534828	34.15637183907911	27.817512200045535	20.762024585527072
18	18.21028442126364	33.78456092746166	26.669217389671452	21.335937261603245
19	18.211927525039727	35.189414656016226	29.8516746748415	16.746983144102547
20	19.24121467620293	37.52168310161658	26.04530741298005	17.19179480920044
21	20.226842212744383	32.29215324055274	28.03393244026731	19.447072106435566
22	19.449654126655133	34.72113007983137	26.448337296343155	19.38087849717034
23	19.40646397025513	33.59912492987468	24.865793630860306	22.12861746900989
24	15.492121317393662	38.255446302194954	26.85840905303235	19.39402332737904
25	16.85824474265474	33.78057053257688	26.791041798212774	22.570142926555608
26	19.620302190257334	37.5261429547231	22.425549794259933	20.42800506075963
27	16.69323017771341	34.318100196468265	27.760942484325962	21.227727141492363
28	18.615661595735443	33.03201939801372	26.452797149449676	21.899521856801158
29	18.606037702189788	37.240947085016536	24.973769021860324	19.179246190933352
30	21.907972104792464	32.04498348680705	23.96349492867756	22.083549479722926
31	20.4683784678292	35.682580517953255	22.36334657987949	21.48569443433805
32	19.901507665079116	33.88643336157907	23.68064635007969	22.531412623262124
33	21.46409935613805	32.489090964572334	23.348739387310076	22.69807029197954
34	17.337326857939594	34.301434429596526	26.565232393556215	21.796006318907665
35	19.892822687976942	34.8359126150466	23.851998601014497	21.419266095961955
36	18.77598157845938	35.18260751180101	26.38848137307141	19.65292953666821
37	20.149616335268284	35.59666966337498	24.39187555601458	19.861838445342155
38	21.091114798966252	32.73250505254411	26.67062576433667	19.50575438415297
39	18.90602150588114	34.06717477694866	27.115437429434564	19.91136628773564
40	21.796945235351142	34.9687692917988	24.41041915577328	18.823866317076778
41	17.567361386591802	31.279531856261283	30.94574706060471	20.207359696542206
42	19.405994512033388	34.31364034336175	24.761339176523332	21.519025968081536
43	19.047093701513766	32.019163284611395	27.82338042781728	21.11036258605756
44	20.681747229609666	29.518594066517533	25.92183990066264	23.877818803210154
45	22.273445330416433	33.04234747889198	25.155449353673394	19.52875783701819
46	17.765238027054878	33.197268692065926	30.253061454428515	18.784431826450685
47	20.106895637090016	32.913715926135445	25.578665940571284	21.400722496203255
48	18.57458400133326	30.74951352391772	31.197376667456922	19.478525807292094
49	21.08078671808799	31.076021717137337	26.84056964060626	21.002621924168412
50	19.57664257563559	30.176539764285025	26.18567542128007	24.061142238799313
51	17.955133877748384	28.319832497306486	30.375355321191577	23.349678303753553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12794.0
1	9692.5
2	6591.0
3	4015.5
4	1440.0
5	1140.0
6	840.0
7	927.5
8	1015.0
9	1149.5
10	1284.0
11	1585.5
12	1887.0
13	2100.5
14	2314.0
15	2264.0
16	2214.0
17	2401.0
18	2588.0
19	2524.5
20	2461.0
21	2324.5
22	2188.0
23	3115.5
24	4043.0
25	4363.5
26	5573.5
27	6463.0
28	7489.5
29	8516.0
30	9340.0
31	10164.0
32	11969.5
33	13775.0
34	16191.0
35	18607.0
36	18697.5
37	18788.0
38	18095.0
39	17402.0
40	17477.5
41	17553.0
42	20783.0
43	24013.0
44	31624.0
45	39235.0
46	40482.0
47	41729.0
48	39573.5
49	37418.0
50	33960.5
51	30503.0
52	26890.5
53	23278.0
54	20922.5
55	18567.0
56	18044.0
57	17521.0
58	14055.5
59	10590.0
60	9757.0
61	8924.0
62	7700.0
63	6476.0
64	5369.5
65	4263.0
66	3252.5
67	2242.0
68	1903.5
69	1565.0
70	1228.0
71	891.0
72	784.0
73	677.0
74	471.0
75	193.5
76	122.0
77	80.0
78	38.0
79	34.5
80	31.0
81	41.0
82	51.0
83	28.5
84	6.0
85	5.5
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	426023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.60858776672658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34542233442166	18.049272628042427
2	10.603359298456422	5.2186739583640245
3	4.188304016798145	3.092047409733318
4	2.2822596520115694	2.246527478119422
5	1.4682286858268219	1.8065517238397482
6	1.0165676862200732	1.5009777076298902
7	0.7444701275123513	1.2824250930815675
8	0.5640810043799585	1.1104989523061988
9	0.4803654913291283	1.0639004718131628
>10	4.009357086162104	21.92669639340473
>50	0.8132327552807673	14.194166758891006
>100	0.4703993388388752	18.376192668985162
>500	0.003986435074905721	0.7921618396436856
>1k	0.006976261381085013	3.6309461411531783
>5k	0.0019932175374528607	2.9619004759680467
>10k+	9.966087687264303E-4	2.747060299024434
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11201	2.6292007708504	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC	6065	1.4236320574241297	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGT	6012	1.4111914145480409	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	4448	1.044075085147985	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2484	0.5830671114000887	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2147	0.5039634010370333	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	2145	0.503493942815294	TruSeq Adapter, Index 14 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	1227	0.2880126190370003	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1202	0.2821443912652603	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	1152	0.2704079357217803	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	872	0.20468378467829199	No Hit
AACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG	851	0.19975447335003038	TruSeq Adapter, Index 15 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	777	0.18238451914567994	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTATC	730	0.1713522509348087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6504343662196642	0.0
2	0.0	0.0	0.0	0.8145100147175153	0.0
3	0.0	0.0	0.0	1.215192606971924	0.0
4	0.0	0.0	0.0	3.139736586991782	0.0
5	0.0	0.0	0.0	3.3258767719113758	0.0
6	0.0	0.0	0.0	4.307044455346308	0.0
7	0.0	0.0	0.0	5.167796104905134	0.0
8	0.0	0.0	0.0	5.772458294505226	0.0
9	0.0	0.0	0.0	8.244860019294732	0.0
10	0.0	0.0	0.0	9.509111010438403	0.0
11	0.0	0.0	0.0	12.261779293606214	0.0
12	0.0	0.0	0.0	12.907049619386747	0.0
13	0.0	0.0	0.0	13.207033423078096	0.0
14	0.0	0.0	0.0	13.676726373928169	0.0
15	0.0	0.0	0.0	13.92906016811299	0.0
16	0.0	0.0	0.0	14.342887590576096	0.0
17	0.0	0.0	0.0	14.878774150691395	0.0
18	0.0	0.0	0.0	15.871913018780676	0.0
19	0.0	0.0	0.0	16.208045105545946	0.0
20	0.0	0.0	0.0	16.560373500961216	0.0
21	0.0	0.0	0.0	16.897209775059093	0.0
22	0.0	0.0	0.0	17.285686453548283	0.0
23	0.0	0.0	0.0	17.667121258711383	0.0
24	0.0	0.0	0.0	17.937059736211424	0.0
25	0.0	0.0	0.0	18.160756578870156	0.0
26	0.0	0.0	0.0	18.356285928224533	0.0
27	0.0	0.0	0.0	18.597352725087614	0.0
28	0.0	0.0	0.0	18.844757207944173	0.0
29	0.0	0.0	0.0	19.14004642941813	0.0
30	0.0	0.0	0.0	19.503172363933402	0.0
31	0.0	0.0	0.0	19.753393596120397	0.0
32	0.0	0.0	0.0	20.000798078976956	0.0
33	0.0	0.0	0.0	20.241864875840037	0.0
34	0.0	0.0	0.0	20.4923208371379	0.0
35	0.0	0.0	0.0	20.77469995751403	0.0
36	0.0	0.0	0.0	21.025390647922766	0.0
37	0.0	0.0	0.0	21.285235773655412	0.0
38	0.0	0.0	0.0	21.55705208404241	0.0
39	2.3472911086960094E-4	0.0	0.0	21.837788100642452	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGT	30	2.1622727E-6	45.000004	41
ATCTCGC	30	2.1622727E-6	45.000004	29
GTCGAGC	30	2.1622727E-6	45.000004	27
ATAGGCG	30	2.1622727E-6	45.000004	26
CAACGAC	30	2.1622727E-6	45.000004	12
CGTTGTC	30	2.1622727E-6	45.000004	45
CTATACG	30	2.1622727E-6	45.000004	1
GACCGAG	30	2.1622727E-6	45.000004	1
CTCCACG	30	2.1622727E-6	45.000004	1
CGTTACT	30	2.1622727E-6	45.000004	31
TGCGAAG	30	2.1622727E-6	45.000004	1
CGGGCAC	30	2.1622727E-6	45.000004	6
GAGCCGA	30	2.1622727E-6	45.000004	41
ACAGACG	30	2.1622727E-6	45.000004	1
TTCGGTG	30	2.1622727E-6	45.000004	30
ACCGTTG	30	2.1622727E-6	45.000004	43
TCGAGCG	30	2.1622727E-6	45.000004	1
TCGGTGA	30	2.1622727E-6	45.000004	31
TCGAACG	30	2.1622727E-6	45.000004	1
TACCGTG	30	2.1622727E-6	45.000004	27
>>END_MODULE