FastQCFastQC Report
Sat 18 Jun 2016
SRR3553382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580249
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239974.135638320789868No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56150.9676880098026882No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGT46290.797760961242501No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44810.7722546699778888No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT40990.7064208641462544No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC30400.5239130097596032No Hit
AATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT28790.49616630101904524No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23370.40275812625269497No Hit
CTACAGGGATTTACACACTTTTTTTTTTAATTATTTTTTTTAAAGATTTAT8640.14890159224746619No Hit
AATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT8550.1473505339948884No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT7340.12649741748800947No Hit
TCTACAGGGGGATTTTGGAACATATTTCCTGACATGAAAGCAACTTCCCAA6970.1201208446718564No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA6230.10736769903955026No Hit
AACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG6090.10495494175776263No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTAT6040.10409324272855273No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC6030.10392090292271076No Hit
AAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT6010.10357622331102682No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.10254218447597496No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTCG207.0303946E-445.00000434
CGGGTTA600.045.0000046
CTGCTAC207.0303946E-445.00000421
TTTAGCG302.1636351E-645.0000041
AGGTATC207.0303946E-445.00000433
AACGTAG351.2105374E-745.0000041
CGGGTCC351.2105374E-745.0000046
TTAGGCG207.0303946E-445.0000041
CGTGGAC207.0303946E-445.00000417
TCGTTGA700.045.00000424
TTGGCCG600.045.0000041
GGTACCC207.0303946E-445.00000414
CGAACAG302.1636351E-645.0000041
ACGCCTA302.1636351E-645.00000421
GCGACTC207.0303946E-445.00000436
GTCGATG302.1636351E-645.0000041
CGAGTTG207.0303946E-445.0000041
AGTCACG351.2105374E-745.0000041
GTACAAT302.1636351E-645.00000435
TACCACG406.8048394E-945.0000041