##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580249
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.427683632371618	31.0	31.0	34.0	30.0	34.0
2	31.631103198799135	31.0	31.0	34.0	30.0	34.0
3	31.810712297651527	31.0	31.0	34.0	30.0	34.0
4	35.56343914422946	37.0	35.0	37.0	33.0	37.0
5	35.27090438759912	37.0	35.0	37.0	33.0	37.0
6	33.915407006302466	37.0	35.0	37.0	31.0	37.0
7	34.74747909949004	37.0	35.0	37.0	32.0	37.0
8	35.4489555346067	37.0	35.0	37.0	32.0	37.0
9	37.05991738029708	39.0	37.0	39.0	33.0	39.0
10	36.462663442763365	38.0	35.0	39.0	32.0	39.0
11	36.53856189325617	38.0	35.0	39.0	32.0	39.0
12	36.5501327878204	38.0	35.0	39.0	32.0	39.0
13	36.5630272520935	39.0	35.0	39.0	32.0	39.0
14	37.645160956761664	39.0	37.0	41.0	32.0	41.0
15	37.68876120424163	39.0	37.0	41.0	32.0	41.0
16	37.54825083714061	39.0	36.0	41.0	32.0	41.0
17	37.46258933664685	39.0	36.0	40.0	32.0	41.0
18	37.26297847992844	39.0	36.0	40.0	32.0	41.0
19	37.12283002641969	39.0	35.0	40.0	32.0	41.0
20	37.0617648630157	39.0	35.0	40.0	31.0	41.0
21	37.00409307038875	39.0	35.0	40.0	31.0	41.0
22	36.95426618572372	39.0	35.0	40.0	31.0	41.0
23	36.76450627230724	39.0	35.0	40.0	31.0	41.0
24	36.63569605462482	38.0	35.0	40.0	31.0	41.0
25	36.66804940637554	38.0	35.0	40.0	31.0	41.0
26	36.61831903200178	38.0	35.0	40.0	31.0	41.0
27	36.570301715298086	38.0	35.0	40.0	31.0	41.0
28	36.407294109942455	38.0	35.0	40.0	30.0	41.0
29	36.25341879089839	38.0	35.0	40.0	30.0	41.0
30	36.22666475943948	38.0	35.0	40.0	30.0	41.0
31	36.05182257961668	38.0	35.0	40.0	30.0	41.0
32	36.0159035172831	38.0	35.0	40.0	30.0	41.0
33	35.8651423785306	38.0	35.0	40.0	29.0	41.0
34	35.83184977483804	38.0	35.0	40.0	29.0	41.0
35	35.7062157797773	38.0	35.0	40.0	29.0	41.0
36	35.59130993763022	38.0	34.0	40.0	28.0	41.0
37	35.52763382616773	38.0	34.0	40.0	28.0	41.0
38	35.426389360429745	38.0	34.0	40.0	27.0	41.0
39	35.29495613090242	38.0	34.0	40.0	26.0	41.0
40	35.14597353894621	38.0	34.0	40.0	26.0	41.0
41	35.12878264331347	38.0	34.0	40.0	26.0	41.0
42	35.115703775448125	38.0	34.0	40.0	26.0	41.0
43	35.072529207288596	38.0	34.0	40.0	25.0	41.0
44	34.920073968244665	38.0	34.0	40.0	25.0	41.0
45	34.70165739191278	37.0	33.0	40.0	24.0	41.0
46	34.508254215000804	37.0	33.0	40.0	24.0	41.0
47	34.41188696576815	37.0	33.0	40.0	23.0	41.0
48	34.321475780225384	37.0	33.0	40.0	23.0	41.0
49	34.14301963467408	37.0	33.0	40.0	23.0	41.0
50	34.04864463359696	37.0	33.0	40.0	23.0	41.0
51	32.87369215629842	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	6.0
14	10.0
15	36.0
16	83.0
17	177.0
18	337.0
19	730.0
20	1271.0
21	2146.0
22	2933.0
23	3635.0
24	4561.0
25	5156.0
26	6093.0
27	7274.0
28	9196.0
29	11208.0
30	14423.0
31	18493.0
32	23784.0
33	30748.0
34	41997.0
35	62789.0
36	61514.0
37	76187.0
38	101675.0
39	93768.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.81829697250663	37.30208927546622	20.710419147641787	25.16919460438536
2	18.394861516349014	39.158533663995975	21.247602322451222	21.199002497203786
3	17.1733169725411	37.62574343083745	22.106543914767627	23.094395681853825
4	20.46966043888055	36.08967874136793	18.701109351330206	24.73955146842132
5	15.3704702636282	42.22308009147797	16.77486734143445	25.63158230345938
6	17.593653758989674	42.99533476145586	19.18331612807605	20.227695351478417
7	75.85036768697576	14.992184389805066	5.175709049046185	3.9817388741729847
8	77.97221537650215	12.287655816726957	3.9250390780509745	5.8150897287199115
9	71.18978231759124	17.409594846350444	7.34219274828565	4.058430087772663
10	25.226928439342423	51.540804034130176	12.085673564280162	11.146593962247241
11	17.2817187104157	34.52535032374032	32.56412333325866	15.628807632585321
12	20.953418273878974	30.510005187428156	33.150768032344736	15.385808506348136
13	22.04501860408204	33.060806653695224	26.891730963775895	18.00244377844684
14	15.341861855858433	35.60126773161178	29.16523768244323	19.891632730086567
15	13.90851169067073	38.561548576559375	27.434084332760587	20.095855400009306
16	16.464655690918896	37.61712644054535	27.13834922593576	18.779868642599986
17	15.586239700542354	36.54741326568421	28.000909954174848	19.865437079598586
18	16.782794972503183	36.97826278028915	26.334901051100474	19.90404119610719
19	17.029585574468893	38.99929168339799	27.873723177463468	16.09739956466965
20	17.580900613357368	37.97852301339597	26.760063352112628	17.680513021134033
21	17.625019603652913	38.09985023670872	25.73222875007109	18.54290140956727
22	17.322907924011933	36.26356960546248	27.33464426478977	19.078878205735812
23	16.93790079776096	36.25029944041265	25.819949711244654	20.991850050581736
24	14.03500910815874	38.14396922700427	26.67268707055075	21.148334594286247
25	15.653279885014882	38.69545660569859	25.003231371359536	20.648032137926993
26	16.43484090450824	38.17274997457988	24.46915031305526	20.923258807856627
27	14.931520778148691	37.65090504249038	25.848902798626106	21.568671380734823
28	16.277322321968672	35.93922609086789	26.77695265308514	21.0064989340783
29	18.02622667165303	36.2180718967202	24.34885712857756	21.40684430304921
30	18.26681304060843	35.989721653979586	24.712494118904125	21.03097118650786
31	17.991414030872953	38.18774353768813	22.893964487659606	20.92687794377931
32	19.945747429120946	35.309151760709625	22.524640283740258	22.22046052642917
33	17.092144923989526	35.54853175102413	26.01848516757461	21.34083815741173
34	16.815194856001476	35.22539461507043	24.710598381039866	23.248812147888234
35	18.948072293101756	33.78480617803736	26.201682381184625	21.06543914767626
36	18.36470205032667	35.500104265582536	27.359978216248543	18.775215467842255
37	19.131958865935143	36.42591370256562	26.261656633617637	18.180470797881597
38	19.339973011586405	33.123883022633386	30.883465546687717	16.652678419092492
39	18.31765328333181	34.39868056644647	27.610560293942772	19.67310585627894
40	18.908089458146414	35.821173323866134	26.056744604471525	19.21399261351592
41	16.101535720009856	32.91311144008865	30.455718148587934	20.529634691313557
42	18.829502506682477	33.463564779947916	25.590393089863145	22.11653962350646
43	17.817006147360875	33.68347037220228	26.980485963784513	21.519037516652332
44	19.712399332010914	31.795143119591764	25.316717478186092	23.175740070211237
45	21.008567011748404	33.5337070809256	25.740156381139823	19.71756952618617
46	17.735661759003463	34.03711165379001	28.282168517308946	19.945058069897577
47	17.786502001726845	34.37834446935712	26.44950702198539	21.385646506930645
48	17.14126176865449	32.35714322644244	29.692252808707988	20.809342196195082
49	18.28094490468747	31.70707747880651	28.263555818278014	21.748421798228
50	18.134111390110107	30.52861788645909	27.34429529391692	23.99297542951388
51	17.190378613319453	29.751537701917623	29.957828449510473	23.10025523525245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27108.0
1	20445.5
2	13783.0
3	8513.5
4	3244.0
5	2597.5
6	1951.0
7	2034.5
8	2118.0
9	2405.5
10	2693.0
11	3011.0
12	3329.0
13	3862.5
14	4396.0
15	4600.0
16	4804.0
17	5022.0
18	5240.0
19	4615.0
20	3990.0
21	4039.5
22	4089.0
23	4610.5
24	5132.0
25	5684.5
26	6521.0
27	6805.0
28	8724.0
29	10643.0
30	11585.5
31	12528.0
32	15470.0
33	18412.0
34	21200.0
35	23988.0
36	24041.0
37	24094.0
38	24980.5
39	25867.0
40	26134.5
41	26402.0
42	29573.0
43	32744.0
44	41028.5
45	49313.0
46	48773.5
47	48234.0
48	50951.5
49	53669.0
50	48926.0
51	44183.0
52	38883.0
53	33583.0
54	27923.5
55	22264.0
56	19930.5
57	17597.0
58	15050.0
59	12503.0
60	11331.5
61	10160.0
62	8626.5
63	7093.0
64	5945.0
65	4797.0
66	3856.0
67	2915.0
68	2251.0
69	1587.0
70	1277.0
71	967.0
72	719.5
73	472.0
74	562.0
75	537.0
76	422.0
77	256.0
78	90.0
79	81.5
80	73.0
81	59.0
82	45.0
83	30.0
84	15.0
85	10.0
86	5.0
87	5.5
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	580249.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.30701738813024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.98306288100267	20.74870819294933
2	10.14055208228315	5.5381646407229255
3	3.9244264924597227	3.214931474041098
4	2.080634055514567	2.2726364132908885
5	1.2570135475479634	1.7162645400003758
6	0.8592337481160055	1.4077866540163246
7	0.6530911650625627	1.2483780260278343
8	0.5027203064034551	1.0982233718660246
9	0.4265265568167178	1.0482451293144088
>10	3.084904266778536	17.883019046067275
>50	0.6119866502281414	11.778849255388277
>100	0.46185271108693415	21.613115684070316
>500	0.00890625062702077	1.558758415582907
>1k	0.0038169645544374724	3.7288252970564026
>5k	6.36160759072912E-4	0.9754183108768553
>10k+	6.36160759072912E-4	4.168675548728744
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23997	4.135638320789868	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5615	0.9676880098026882	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGT	4629	0.797760961242501	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4481	0.7722546699778888	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	4099	0.7064208641462544	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC	3040	0.5239130097596032	No Hit
AATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	2879	0.49616630101904524	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2337	0.40275812625269497	No Hit
CTACAGGGATTTACACACTTTTTTTTTTAATTATTTTTTTTAAAGATTTAT	864	0.14890159224746619	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT	855	0.1473505339948884	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	734	0.12649741748800947	No Hit
TCTACAGGGGGATTTTGGAACATATTTCCTGACATGAAAGCAACTTCCCAA	697	0.1201208446718564	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	623	0.10736769903955026	No Hit
AACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	609	0.10495494175776263	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTAT	604	0.10409324272855273	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	603	0.10392090292271076	No Hit
AAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	601	0.10357622331102682	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	595	0.10254218447597496	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7233980584197475E-4	0.0	0.0	0.87359047581297	0.0
2	1.7233980584197475E-4	0.0	0.0	1.1515745826360753	0.0
3	1.7233980584197475E-4	0.0	0.0	1.5379604273337826	0.0
4	1.7233980584197475E-4	0.0	0.0	3.3146114857587	0.0
5	1.7233980584197475E-4	0.0	0.0	3.5464085246161563	0.0
6	3.446796116839495E-4	0.0	0.0	4.753821204345031	0.0
7	3.446796116839495E-4	0.0	0.0	5.714960301525724	0.0
8	3.446796116839495E-4	0.0	0.0	6.370196243336912	0.0
9	3.446796116839495E-4	0.0	0.0	8.307985020224077	0.0
10	3.446796116839495E-4	0.0	0.0	10.013976758253785	0.0
11	3.446796116839495E-4	0.0	0.0	11.993299428348864	0.0
12	3.446796116839495E-4	0.0	0.0	12.817256040079345	0.0
13	3.446796116839495E-4	0.0	0.0	13.157454816811404	0.0
14	3.446796116839495E-4	0.0	0.0	13.580376700347609	0.0
15	3.446796116839495E-4	0.0	0.0	13.893518127562478	0.0
16	3.446796116839495E-4	0.0	0.0	14.487056418882238	0.0
17	3.446796116839495E-4	0.0	0.0	15.31980236071066	0.0
18	3.446796116839495E-4	0.0	0.0	16.605457312291794	0.0
19	3.446796116839495E-4	0.0	0.0	17.053540807480925	0.0
20	3.446796116839495E-4	0.0	0.0	17.565045351219908	0.0
21	3.446796116839495E-4	0.0	0.0	18.061211652238953	0.0
22	3.446796116839495E-4	0.0	0.0	18.633207467828466	0.0
23	3.446796116839495E-4	0.0	0.0	19.180731030988422	0.0
24	3.446796116839495E-4	0.0	0.0	19.53488933199368	0.0
25	3.446796116839495E-4	0.0	0.0	19.86061156503501	0.0
26	3.446796116839495E-4	0.0	0.0	20.15807006991826	0.0
27	3.446796116839495E-4	0.0	0.0	20.54324953597507	0.0
28	3.446796116839495E-4	0.0	0.0	20.91222906028274	0.0
29	3.446796116839495E-4	0.0	0.0	21.289825574882506	0.0
30	3.446796116839495E-4	0.0	0.0	21.762726002112885	0.0
31	3.446796116839495E-4	0.0	0.0	22.103958817679995	0.0
32	3.446796116839495E-4	0.0	0.0	22.44295121577116	0.0
33	3.446796116839495E-4	0.0	0.0	22.788837206096005	0.0
34	3.446796116839495E-4	0.0	0.0	23.128174283798852	0.0
35	3.446796116839495E-4	0.0	0.0	23.48336662363916	0.0
36	3.446796116839495E-4	0.0	0.0	23.820290944060222	0.0
37	3.446796116839495E-4	0.0	0.0	24.17100244894864	0.0
38	3.446796116839495E-4	0.0	0.0	24.532916041216787	0.0
39	5.170194175259242E-4	0.0	0.0	24.916544449021025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCTCG	20	7.0303946E-4	45.000004	34
CGGGTTA	60	0.0	45.000004	6
CTGCTAC	20	7.0303946E-4	45.000004	21
TTTAGCG	30	2.1636351E-6	45.000004	1
AGGTATC	20	7.0303946E-4	45.000004	33
AACGTAG	35	1.2105374E-7	45.000004	1
CGGGTCC	35	1.2105374E-7	45.000004	6
TTAGGCG	20	7.0303946E-4	45.000004	1
CGTGGAC	20	7.0303946E-4	45.000004	17
TCGTTGA	70	0.0	45.000004	24
TTGGCCG	60	0.0	45.000004	1
GGTACCC	20	7.0303946E-4	45.000004	14
CGAACAG	30	2.1636351E-6	45.000004	1
ACGCCTA	30	2.1636351E-6	45.000004	21
GCGACTC	20	7.0303946E-4	45.000004	36
GTCGATG	30	2.1636351E-6	45.000004	1
CGAGTTG	20	7.0303946E-4	45.000004	1
AGTCACG	35	1.2105374E-7	45.000004	1
GTACAAT	30	2.1636351E-6	45.000004	35
TACCACG	40	6.8048394E-9	45.000004	1
>>END_MODULE