SRR3553380_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686531
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26729	3.8933420340814906	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	10327	1.5042292336398502	TruSeq Adapter, Index 10 (96% over 25bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6736	0.9811647252636807	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5090	0.7414086181104713	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	3309	0.48198843169499994	RNA PCR Primer, Index 35 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3217	0.4685877258273843	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGT	2980	0.4340663422336355	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2597	0.3782786210673662	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	2384	0.3472530737869084	TruSeq Adapter, Index 10 (95% over 22bp)
AAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	1355	0.19736909185455576	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC	1330	0.19372759569487757	No Hit
AAATACGGGGTATATACTCAATATACATAATTATTACCACCCAACGCGGCA	1284	0.18702724276106977	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1282	0.18673592306829553	RNA PCR Primer, Index 10 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1089	0.15862357271558022	TruSeq Adapter, Index 10 (95% over 23bp)
GAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	992	0.144494567616029	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	948	0.13808553437499546	RNA PCR Primer, Index 10 (95% over 22bp)
AACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	912	0.1328417799050589	TruSeq Adapter, Index 10 (95% over 22bp)
TGCACGGGATTCTGCTTTTCTGCTGTAGGTGCTCTGACTTGCTCTCTGTCA	841	0.12249993081157295	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	825	0.12016937326937896	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	784	0.11419731956750678	RNA PCR Primer, Index 35 (95% over 23bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	774	0.11274072110363552	No Hit
CTATTTGGGGTATATTTTGTGATTTCTTTTACTCCTATTAAACCTGTCTCT	767	0.11172110217892565	No Hit
GAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	735	0.10705998709453761	No Hit
CTATTTGGGGTATATTTTGTGATTTCTTTTACTCCTGTCTCTTATACACAT	732	0.10662300755537622	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	729	0.10618602801621485	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGCG	55	1.8189894E-12	45.000004	1
CTATACG	55	1.8189894E-12	45.000004	1
TATACGG	110	0.0	45.000004	2
TAGCTCG	55	1.8189894E-12	45.000004	1
ATGCACG	75	0.0	45.000004	1
CTTCGTC	25	3.889159E-5	45.0	4
TCGCCCA	20	7.0313E-4	45.0	38
CAGGTCG	20	7.0313E-4	45.0	34
CAGAACG	20	7.0313E-4	45.0	1
CGGCGGA	20	7.0313E-4	45.0	31
AACCGCC	20	7.0313E-4	45.0	33
TCGTTGG	40	6.8084773E-9	45.0	34
CTGTCGG	20	7.0313E-4	45.0	2
AGCCGAT	45	3.8380676E-10	45.0	44
AATCCCG	35	1.210974E-7	45.0	1
CCGGGTC	35	1.210974E-7	45.0	5
GTCGCAG	25	3.889159E-5	45.0	39
TCGCAGT	25	3.889159E-5	45.0	40
AGATCAC	25	3.889159E-5	45.0	45
CGAACAT	35	1.210974E-7	45.0	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content