##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686531
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18505209524406	31.0	31.0	34.0	28.0	34.0
2	31.386119490598386	31.0	31.0	34.0	28.0	34.0
3	31.602100997624287	31.0	31.0	34.0	30.0	34.0
4	35.39598357539572	37.0	35.0	37.0	33.0	37.0
5	35.090922332713305	37.0	35.0	37.0	32.0	37.0
6	33.774119449813625	36.0	35.0	37.0	30.0	37.0
7	34.646521424378506	37.0	35.0	37.0	32.0	37.0
8	35.32050555619484	37.0	35.0	37.0	32.0	37.0
9	36.91193260027588	39.0	37.0	39.0	33.0	39.0
10	36.28903865957983	38.0	35.0	39.0	32.0	39.0
11	36.40323160935194	38.0	35.0	39.0	32.0	39.0
12	36.48541289468356	38.0	35.0	39.0	32.0	39.0
13	36.41849093485946	38.0	35.0	39.0	32.0	39.0
14	37.48414419742153	39.0	36.0	40.0	32.0	41.0
15	37.47508706817318	39.0	36.0	40.0	32.0	41.0
16	37.36989007051393	39.0	36.0	40.0	32.0	41.0
17	37.315461355714454	39.0	36.0	40.0	32.0	41.0
18	37.147273757485095	38.0	36.0	40.0	32.0	41.0
19	36.95538001925623	39.0	35.0	40.0	32.0	41.0
20	36.87472087931936	39.0	35.0	40.0	31.0	41.0
21	36.79169622347716	38.0	35.0	40.0	31.0	41.0
22	36.755750286585744	38.0	35.0	40.0	31.0	41.0
23	36.569090106637574	38.0	35.0	40.0	31.0	41.0
24	36.30960000349584	38.0	35.0	40.0	30.0	41.0
25	36.39002026128463	38.0	35.0	40.0	30.0	41.0
26	36.32576824644481	38.0	35.0	40.0	30.0	41.0
27	36.18921068385841	38.0	35.0	40.0	30.0	41.0
28	36.16533703503556	38.0	35.0	40.0	30.0	41.0
29	35.95401227329866	38.0	35.0	40.0	30.0	41.0
30	35.86379202104494	38.0	35.0	40.0	30.0	41.0
31	35.77887378719971	38.0	34.0	40.0	29.0	41.0
32	35.69990867127632	38.0	34.0	40.0	29.0	41.0
33	35.6276249725067	38.0	34.0	40.0	29.0	41.0
34	35.65250221767116	38.0	35.0	40.0	29.0	41.0
35	35.551274450825964	38.0	34.0	40.0	28.0	41.0
36	35.54858993985705	38.0	35.0	40.0	28.0	41.0
37	35.46538612240379	38.0	34.0	40.0	27.0	41.0
38	35.36973712767522	38.0	34.0	40.0	27.0	41.0
39	35.27618126493924	38.0	34.0	40.0	27.0	41.0
40	35.12200905712925	38.0	34.0	40.0	26.0	41.0
41	35.036314456302776	38.0	34.0	40.0	25.0	41.0
42	34.88319682578063	38.0	33.0	40.0	25.0	41.0
43	34.778783478094944	38.0	33.0	40.0	24.0	41.0
44	34.666207352617725	38.0	33.0	40.0	24.0	41.0
45	34.48142472808948	37.0	33.0	40.0	23.0	41.0
46	34.28578753180847	37.0	33.0	40.0	23.0	41.0
47	34.260665578102085	37.0	33.0	40.0	23.0	41.0
48	34.11126518685973	37.0	33.0	40.0	23.0	41.0
49	33.941395217404605	37.0	33.0	40.0	22.0	41.0
50	33.84139973285984	37.0	33.0	40.0	22.0	41.0
51	32.50720797749847	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	4.0
11	0.0
12	4.0
13	5.0
14	21.0
15	35.0
16	89.0
17	259.0
18	492.0
19	1042.0
20	1646.0
21	2617.0
22	3468.0
23	4520.0
24	5506.0
25	6512.0
26	7442.0
27	9364.0
28	11380.0
29	14205.0
30	18037.0
31	23352.0
32	29701.0
33	37918.0
34	51703.0
35	77464.0
36	76734.0
37	95154.0
38	118212.0
39	89628.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.24714543116043	36.049938021735365	21.427000383085396	25.275916164018813
2	16.77579016825169	39.40113410756397	21.373834539154096	22.449241185030246
3	17.419606689282784	38.68536162241764	20.918064879808778	22.976966808490804
4	16.74520160051039	37.5645091044687	20.22049987546083	25.46978941956008
5	14.062584209598691	41.40555925369721	18.646645235247934	25.885211301456163
6	17.604740354040825	42.4012899635996	19.409320190930927	20.584649491428646
7	71.36618739721877	14.06316684898424	8.110194586988788	6.460451166808199
8	74.01705094161808	12.67546549245409	6.2489530698540925	7.05853049607374
9	65.72638380495563	17.7362711953284	11.647106976960982	4.890238022754981
10	23.27964796928325	48.12339136907146	13.006113343752867	15.590847317892417
11	14.764373349491866	33.27584624729255	35.220696516253454	16.739083886962135
12	19.589647080758187	29.422997650506677	33.96569127978198	17.021663988953158
13	19.608582860788516	32.61950297947216	28.838901666494305	18.933012493245023
14	13.71911829181785	34.6047010258823	31.63309449973854	20.04308618256131
15	12.598557093561688	37.810819904709334	29.33006666851169	20.26055633321729
16	13.459989425095154	37.15374833765701	27.661970107686322	21.72429212956152
17	13.558018501713686	36.629664210356125	29.937031248406846	19.875286039523342
18	13.860845322352521	34.59275691847855	27.307725361272833	24.23867239789609
19	14.176781529166199	40.25513778693169	28.689891643640276	16.878189040261837
20	16.421691081684585	37.083248972005634	27.516018941606426	18.979041004703355
21	15.226260722385444	38.95672591623685	25.265137335386168	20.551876025991543
22	14.397019216903534	36.03784825448523	27.201830652949393	22.363301875661843
23	17.191649029686936	36.236236965264496	23.154817480929484	23.417296524119084
24	12.07243372841139	37.58592110188761	26.636816108813733	23.704829060887274
25	14.344581672204168	36.29785108028625	25.578451664964874	23.77911558254471
26	17.100465965848592	37.02469371375801	25.450271000144202	20.424569320249194
27	12.42828073313514	35.85344288895913	25.63962880044747	26.078647577458263
28	16.367942598367733	33.577507789160286	27.794520567898612	22.260029044573372
29	16.590510843647262	34.4146149263471	28.23033482828889	20.764539401716746
30	15.444896151812518	34.50026291602273	22.817760596389675	27.237080335775076
31	14.873763894128597	35.46890089449712	27.564378010606948	22.092957200767334
32	15.424795093011095	32.617026762083576	25.10986393913749	26.84831420576784
33	13.881092041000334	31.661061190244872	28.103173782392933	26.35467298636187
34	12.673134934911898	30.182176769876378	31.077984825157202	26.06670347005452
35	13.910078350431371	29.381047614747185	27.765242938774797	28.943631096046644
36	15.073026563986186	29.162849164859274	33.42004949521581	22.34407477593874
37	14.147358240196	32.011081801113136	28.602204416115224	25.23935554257564
38	15.66775571678482	30.446112411529853	32.01982139189636	21.866310479788968
39	15.07229826475425	29.860705488899992	30.515883477949284	24.551112768396475
40	16.62925636278624	31.200193436276003	26.13560057739563	26.034949623542126
41	15.283505042015582	27.96741880556013	30.346626736447448	26.402449415976847
42	18.361588915868328	30.663728222032216	26.81685167894822	24.157831183151234
43	14.899400027092732	30.250491237831937	29.474561236127723	25.375547498947608
44	17.876687287245588	28.068798058645566	26.810879625246347	27.2436350288625
45	19.507640587242236	29.561957143959994	28.890028272576185	22.040373996221582
46	14.558701646393244	30.079632238019844	32.08871849923747	23.272947616349445
47	16.031614013059862	29.068898563939577	27.450326350885828	27.449161072114737
48	16.37799312776845	27.068988873044336	34.14397893175982	22.4090390674274
49	16.892026725668615	26.75203304730595	30.254424053684392	26.101516173341043
50	15.870368563109313	26.168228382986346	27.602395230513988	30.35900782339035
51	16.11056164980168	25.319614117934954	34.26458528456836	24.305238947695006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30922.0
1	23430.5
2	15939.0
3	9894.5
4	3850.0
5	3062.0
6	2274.0
7	2475.5
8	2677.0
9	2849.5
10	3022.0
11	3616.0
12	4210.0
13	4656.5
14	5103.0
15	5557.0
16	6011.0
17	5697.5
18	5384.0
19	5273.0
20	5162.0
21	5078.5
22	4995.0
23	5855.0
24	6715.0
25	5863.0
26	5813.0
27	6615.0
28	7906.5
29	9198.0
30	10401.0
31	11604.0
32	13231.5
33	14859.0
34	16992.5
35	19126.0
36	20390.0
37	21654.0
38	23689.0
39	25724.0
40	27346.5
41	28969.0
42	35816.0
43	42663.0
44	54646.0
45	66629.0
46	70033.5
47	73438.0
48	77680.0
49	81922.0
50	72213.5
51	62505.0
52	52575.0
53	42645.0
54	33170.5
55	23696.0
56	20024.0
57	16352.0
58	14599.5
59	12847.0
60	11101.5
61	9356.0
62	7569.0
63	5782.0
64	5037.0
65	4292.0
66	3320.5
67	2349.0
68	1769.5
69	1190.0
70	959.5
71	729.0
72	717.5
73	706.0
74	477.0
75	160.0
76	72.0
77	50.0
78	28.0
79	23.0
80	18.0
81	19.0
82	20.0
83	15.5
84	11.0
85	8.5
86	6.0
87	3.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686531.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.770068567119694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62512965647875	21.182000284799713
2	10.644870784508315	6.125073247168668
3	4.581155494052318	3.9540047304156687
4	2.5564049495606587	2.9419178273673725
5	1.548203677606895	2.2270962980308617
6	1.0839604497144435	1.871136987739826
7	0.7828461233833593	1.5765775653070162
8	0.6132798846583766	1.41152834659654
9	0.4890365532941325	1.2662653653090066
>10	3.235721208386047	18.283326781670116
>50	0.49672997820499937	10.041175218561728
>100	0.32101947761583344	16.029441378017953
>500	0.014427841690599255	2.7563458113282326
>1k	0.005152800603785448	3.0875283285394017
>5k	0.0010305601207570897	1.7531622419602906
>10k+	0.0010305601207570897	5.493419587187597
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26729	3.8933420340814906	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	10327	1.5042292336398502	TruSeq Adapter, Index 10 (96% over 25bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6736	0.9811647252636807	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5090	0.7414086181104713	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	3309	0.48198843169499994	RNA PCR Primer, Index 35 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3217	0.4685877258273843	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGT	2980	0.4340663422336355	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2597	0.3782786210673662	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	2384	0.3472530737869084	TruSeq Adapter, Index 10 (95% over 22bp)
AAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	1355	0.19736909185455576	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC	1330	0.19372759569487757	No Hit
AAATACGGGGTATATACTCAATATACATAATTATTACCACCCAACGCGGCA	1284	0.18702724276106977	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1282	0.18673592306829553	RNA PCR Primer, Index 10 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1089	0.15862357271558022	TruSeq Adapter, Index 10 (95% over 23bp)
GAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	992	0.144494567616029	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	948	0.13808553437499546	RNA PCR Primer, Index 10 (95% over 22bp)
AACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	912	0.1328417799050589	TruSeq Adapter, Index 10 (95% over 22bp)
TGCACGGGATTCTGCTTTTCTGCTGTAGGTGCTCTGACTTGCTCTCTGTCA	841	0.12249993081157295	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	825	0.12016937326937896	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	784	0.11419731956750678	RNA PCR Primer, Index 35 (95% over 23bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	774	0.11274072110363552	No Hit
CTATTTGGGGTATATTTTGTGATTTCTTTTACTCCTATTAAACCTGTCTCT	767	0.11172110217892565	No Hit
GAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	735	0.10705998709453761	No Hit
CTATTTGGGGTATATTTTGTGATTTCTTTTACTCCTGTCTCTTATACACAT	732	0.10662300755537622	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	729	0.10618602801621485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.91319692774252E-4	0.0	0.0	1.8006470210376515	0.0
2	2.91319692774252E-4	0.0	0.0	2.3439582480616314	0.0
3	2.91319692774252E-4	0.0	0.0	2.997533978800666	0.0
4	2.91319692774252E-4	0.0	0.0	6.20496379624518	0.0
5	2.91319692774252E-4	0.0	0.0	6.637136560475783	0.0
6	2.91319692774252E-4	0.0	0.0	9.147729672804287	0.0
7	2.91319692774252E-4	0.0	0.0	11.247707678167483	0.0
8	2.91319692774252E-4	0.0	0.0	12.430465630830946	0.0
9	2.91319692774252E-4	0.0	0.0	14.640125500523647	0.0
10	2.91319692774252E-4	0.0	0.0	17.56628615459462	0.0
11	2.91319692774252E-4	0.0	0.0	20.25647785751845	0.0
12	2.91319692774252E-4	0.0	0.0	21.690790364892482	0.0
13	2.91319692774252E-4	0.0	0.0	22.1997258681691	0.0
14	2.91319692774252E-4	0.0	0.0	22.719731519771138	0.0
15	2.91319692774252E-4	0.0	0.0	23.16661592848684	0.0
16	2.91319692774252E-4	0.0	0.0	24.136419185732326	0.0
17	2.91319692774252E-4	0.0	0.0	25.56650755756113	0.0
18	2.91319692774252E-4	0.0	0.0	27.469407790762546	0.0
19	2.91319692774252E-4	0.0	0.0	28.208777171023595	0.0
20	2.91319692774252E-4	0.0	0.0	28.922947397859673	0.0
21	2.91319692774252E-4	0.0	0.0	29.653577187337497	0.0
22	2.91319692774252E-4	0.0	0.0	30.53583887690432	0.0
23	2.91319692774252E-4	0.0	0.0	31.308273042295248	0.0
24	2.91319692774252E-4	0.0	0.0	31.906935010946338	0.0
25	2.91319692774252E-4	0.0	0.0	32.40421772651199	0.0
26	2.91319692774252E-4	0.0	0.0	32.854452311694594	0.0
27	2.91319692774252E-4	0.0	0.0	33.35537652341992	0.0
28	2.91319692774252E-4	0.0	0.0	33.81376806000021	0.0
29	2.91319692774252E-4	0.0	0.0	34.32503412081902	0.0
30	2.91319692774252E-4	0.0	0.0	34.99142791804012	0.0
31	2.91319692774252E-4	0.0	0.0	35.48958459268409	0.0
32	2.91319692774252E-4	0.0	0.0	35.93821691955644	0.0
33	2.91319692774252E-4	0.0	0.0	36.39529751751924	0.0
34	2.91319692774252E-4	0.0	0.0	36.83912306946081	0.0
35	2.91319692774252E-4	0.0	0.0	37.44768990766622	0.0
36	2.91319692774252E-4	0.0	0.0	37.89224375883973	0.0
37	2.91319692774252E-4	0.0	0.0	38.326892740458916	0.0
38	2.91319692774252E-4	0.0	0.0	38.742605942047774	0.0
39	4.36979539161378E-4	0.0	0.0	39.1507448316245	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGCG	55	1.8189894E-12	45.000004	1
CTATACG	55	1.8189894E-12	45.000004	1
TATACGG	110	0.0	45.000004	2
TAGCTCG	55	1.8189894E-12	45.000004	1
ATGCACG	75	0.0	45.000004	1
CTTCGTC	25	3.889159E-5	45.0	4
TCGCCCA	20	7.0313E-4	45.0	38
CAGGTCG	20	7.0313E-4	45.0	34
CAGAACG	20	7.0313E-4	45.0	1
CGGCGGA	20	7.0313E-4	45.0	31
AACCGCC	20	7.0313E-4	45.0	33
TCGTTGG	40	6.8084773E-9	45.0	34
CTGTCGG	20	7.0313E-4	45.0	2
AGCCGAT	45	3.8380676E-10	45.0	44
AATCCCG	35	1.210974E-7	45.0	1
CCGGGTC	35	1.210974E-7	45.0	5
GTCGCAG	25	3.889159E-5	45.0	39
TCGCAGT	25	3.889159E-5	45.0	40
AGATCAC	25	3.889159E-5	45.0	45
CGAACAT	35	1.210974E-7	45.0	30
>>END_MODULE