FastQCFastQC Report
Sat 18 Jun 2016
SRR3553379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674874
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA265403.9325859345596363No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT97951.4513820357577858TruSeq Adapter, Index 10 (96% over 25bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66370.9834428352551735No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50790.7525849269641444No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31730.4701618376170959No Hit
AAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT29450.43637775347694535RNA PCR Primer, Index 35 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGT27950.4141513823321094No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT23560.3491022027815563No Hit
AATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT21800.32302326063828213TruSeq Adapter, Index 10 (95% over 22bp)
AAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT13250.19633294511271737No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC12770.18922050634636983RNA PCR Primer, Index 10 (95% over 24bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC12540.18581246277082833No Hit
AAATACGGGGTATATACTCAATATACATAATTATTACCACCCAACGCGGCA12420.18403435307924146No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG10290.15247290605357444TruSeq Adapter, Index 10 (95% over 23bp)
GAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT9830.14565681890249144No Hit
TACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT8890.13172829298506092RNA PCR Primer, Index 10 (95% over 22bp)
AACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT8530.12639396391030028TruSeq Adapter, Index 10 (95% over 22bp)
TGCACGGGATTCTGCTTTTCTGCTGTAGGTGCTCTGACTTGCTCTCTGTCA7870.11661436060657249No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7870.11661436060657249No Hit
CTGTCACTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT7300.10816833957153482TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC7280.10787198795627036RNA PCR Primer, Index 35 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT7270.10772381214863812No Hit
CAAAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT7040.1043157685730966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCA1250.045.0000045
GACTAGG950.045.0000042
AACGTAG406.8084773E-945.01
CGGGTCG351.2109376E-745.06
GAGTGGT207.031215E-445.022
AGCCGGA207.031215E-445.036
CTGTCGG253.889088E-545.02
ACCGGCA253.889088E-545.038
CGAACAT253.889088E-545.030
ACGCCGA253.889088E-545.041
GATCGTA207.031215E-445.021
CATATAG800.045.01
ATTAGCG207.031215E-445.01
CTGTACG453.8380676E-1045.01
GTCTGCG351.2109376E-745.012
CTCCGCA207.031215E-445.018
TTTACGG850.045.02
TTAGCTT351.2109376E-745.025
TGCCACG453.8380676E-1045.01
CGCGGAC253.889088E-545.04
Produced by FastQC (version 0.11.5)