##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448875
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.471195767195766	31.0	31.0	34.0	30.0	34.0
2	31.67920022277917	31.0	31.0	34.0	30.0	34.0
3	31.842570871623504	31.0	31.0	34.0	30.0	34.0
4	35.57763519910888	37.0	35.0	37.0	33.0	37.0
5	35.312991367307156	37.0	35.0	37.0	33.0	37.0
6	33.87893065998329	37.0	35.0	37.0	30.0	37.0
7	34.712187134502926	37.0	35.0	37.0	32.0	37.0
8	35.45259147869674	37.0	35.0	37.0	32.0	37.0
9	37.16747869674185	39.0	37.0	39.0	34.0	39.0
10	36.57851963241437	38.0	35.0	39.0	32.0	39.0
11	36.62236480089112	39.0	35.0	39.0	32.0	39.0
12	36.54025508214982	38.0	35.0	39.0	32.0	39.0
13	36.573201893622944	39.0	35.0	39.0	32.0	39.0
14	37.6863380673907	39.0	37.0	41.0	32.0	41.0
15	37.75807964355333	39.0	37.0	41.0	33.0	41.0
16	37.56592815371763	39.0	36.0	41.0	32.0	41.0
17	37.533197438039544	39.0	36.0	40.0	32.0	41.0
18	37.34950041771094	39.0	36.0	40.0	32.0	41.0
19	37.25500417710944	39.0	35.0	40.0	32.0	41.0
20	37.26688276246171	39.0	35.0	40.0	32.0	41.0
21	37.2689902534113	39.0	35.0	40.0	32.0	41.0
22	37.228335282651074	39.0	35.0	40.0	32.0	41.0
23	37.039073238652186	39.0	35.0	40.0	31.0	41.0
24	36.88478529657477	39.0	35.0	40.0	31.0	41.0
25	36.96514174324701	39.0	35.0	40.0	31.0	41.0
26	36.88390086326928	39.0	35.0	40.0	31.0	41.0
27	36.86802784739627	39.0	35.0	40.0	31.0	41.0
28	36.746486215538845	39.0	35.0	40.0	31.0	41.0
29	36.59100417710944	39.0	35.0	40.0	31.0	41.0
30	36.64184238373712	39.0	35.0	40.0	31.0	41.0
31	36.477152882205516	38.0	35.0	40.0	30.0	41.0
32	36.465140629351154	38.0	35.0	40.0	30.0	41.0
33	36.31941409078251	38.0	35.0	40.0	30.0	41.0
34	36.266684489000276	38.0	35.0	40.0	30.0	41.0
35	36.176182678919524	38.0	35.0	40.0	30.0	41.0
36	36.04396992481203	38.0	35.0	40.0	30.0	41.0
37	36.08499248120301	38.0	35.0	40.0	30.0	41.0
38	35.9332754107491	38.0	35.0	40.0	29.0	41.0
39	35.87514341409078	38.0	35.0	40.0	29.0	41.0
40	35.67313394597605	38.0	35.0	40.0	28.0	41.0
41	35.56902032859928	38.0	34.0	40.0	28.0	41.0
42	35.54293511556669	38.0	34.0	40.0	28.0	41.0
43	35.489274296853246	38.0	34.0	40.0	27.0	41.0
44	35.403780562517404	38.0	34.0	40.0	27.0	41.0
45	35.19761403508772	38.0	34.0	40.0	26.0	41.0
46	34.995484266221105	38.0	34.0	40.0	26.0	41.0
47	34.97944193817878	38.0	34.0	40.0	26.0	41.0
48	34.90588916736285	38.0	33.0	40.0	25.0	41.0
49	34.74105931495405	37.0	33.0	40.0	25.0	41.0
50	34.62227123363965	37.0	33.0	40.0	24.0	41.0
51	33.423235867446394	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	7.0
13	3.0
14	7.0
15	19.0
16	54.0
17	110.0
18	227.0
19	433.0
20	866.0
21	1375.0
22	1900.0
23	2461.0
24	2858.0
25	3310.0
26	3943.0
27	4682.0
28	5813.0
29	7529.0
30	10295.0
31	13068.0
32	17386.0
33	22840.0
34	32420.0
35	45117.0
36	47101.0
37	60695.0
38	82655.0
39	81681.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.04110275689223	38.26120857699805	20.77037037037037	22.927318295739347
2	19.734670008354218	38.05246449456976	20.91183514341409	21.301030353661933
3	18.058702311333892	33.59888610414926	24.78707880813144	23.555332776385406
4	21.82099693678641	34.006349206349206	19.36285157337789	24.809802283486494
5	16.013366750208853	40.89468114731273	17.41865775549986	25.673294346978558
6	16.955945419103312	41.41241993873573	21.65012531328321	19.98150932887775
7	77.76931216931217	13.1885268727374	4.834753550543025	4.207407407407407
8	79.39537733221944	9.553216374269006	3.2552492341966026	7.796157059314954
9	75.56914508493456	13.538290169869116	6.739961013645224	4.1526037315511
10	28.97956001113896	46.84778613199666	11.984628237259816	12.188025619604566
11	19.544862155388472	30.051127819548874	32.751211361737674	17.65279866332498
12	22.653077137287664	28.759899749373435	33.22751322751323	15.359509885825675
13	22.382846003898635	31.478919521024785	26.158952937900303	19.979281537176274
14	16.434196602617654	33.02834864940128	29.841269841269842	20.696184906711224
15	16.21097187412977	35.09997215260373	26.581787802840434	22.107268170426064
16	17.73299916457811	35.13294346978558	28.065274296853243	19.06878306878307
17	17.254469507101085	33.97894736842105	28.302534113060428	20.464049011417433
18	18.27056530214425	33.57415761626288	25.641214146477303	22.514062935115568
19	17.650348092453356	36.1624060150376	27.519465329991643	18.667780562517404
20	19.870788081314398	36.251294903926485	26.133778891673625	17.74413812308549
21	18.57287663603453	34.0807574491785	27.11155666945141	20.234809245335562
22	20.132553606237817	34.31601225285436	25.636313004734056	19.91512113617377
23	18.936675020885545	35.35950988582567	23.257031467557784	22.446783625730994
24	14.67557783347257	38.695850737956	26.263436368699526	20.365135059871903
25	15.44238373712058	36.137900306321356	26.71746031746032	21.702255639097746
26	20.821832358674463	36.204956836535786	22.510275689223057	20.462935115566694
27	15.686995265942635	35.11066555277081	27.138290169869116	22.064049011417435
28	18.17699805068226	34.52297410192147	28.239042049568365	19.060985797827904
29	19.396491228070175	35.147201336675025	24.575884154831524	20.88042328042328
30	19.01576162628794	34.30754664438875	24.539793929267613	22.136897800055692
31	21.276747424115843	37.48148148148148	22.182121971595656	19.059649122807016
32	19.163909774436092	31.45084934558619	24.643163464216094	24.742077415761628
33	18.984349763297132	35.02422723475355	24.98780284043442	21.0036201615149
34	18.411584516847675	33.59688109161794	26.289501531606795	21.7020328599276
35	19.739793929267613	31.51901977165135	25.642105263157895	23.09908103592314
36	18.90949596212754	35.68499025341131	25.76931216931217	19.636201615148984
37	18.243609022556388	34.61030353661933	24.2729044834308	22.873182957393485
38	18.271901977165136	36.154163185742135	26.305096073517127	19.268838763575605
39	19.150543024227236	33.825229741019214	24.31991088833194	22.70431634642161
40	21.7526037315511	35.621498189919244	22.850236702868283	19.775661375661375
41	19.0197716513506	31.73266499582289	28.83519910888332	20.41236424394319
42	22.962740183792814	30.553494848231686	24.742968532442216	21.740796435533277
43	20.823614592035643	32.20629351155667	26.012364243943193	20.957727652464495
44	20.264884433305486	31.519242550821495	23.462211083263714	24.7536619326093
45	22.105485937064884	34.93199665831245	23.73667502088555	19.22584238373712
46	17.570593149540517	32.46872737399053	28.557170704539125	21.403508771929825
47	19.527039821776665	33.95221386800334	25.6969089390142	20.82383737120579
48	18.682483987747144	31.491395154553047	29.086271233639653	20.73984962406015
49	20.460930103035366	31.213143971038708	26.047563352826508	22.278362573099415
50	19.129379003063214	30.23091060985798	25.58084099136731	25.058869395711504
51	17.53806739069897	29.260261765524927	29.428460038986355	23.773210804789752
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13253.0
1	10349.5
2	7446.0
3	4522.0
4	1598.0
5	1309.0
6	1020.0
7	1121.5
8	1223.0
9	1338.5
10	1454.0
11	1711.0
12	1968.0
13	2341.0
14	2714.0
15	2678.0
16	2642.0
17	2583.5
18	2525.0
19	2661.5
20	2798.0
21	2787.5
22	2777.0
23	3074.5
24	3372.0
25	4073.5
26	5222.5
27	5670.0
28	5883.0
29	6096.0
30	7745.0
31	9394.0
32	12756.0
33	16118.0
34	16310.5
35	16503.0
36	17992.5
37	19482.0
38	19471.0
39	19460.0
40	19890.5
41	20321.0
42	21944.0
43	23567.0
44	32412.0
45	41257.0
46	41573.0
47	41889.0
48	41867.5
49	41846.0
50	39259.0
51	36672.0
52	32423.0
53	28174.0
54	24780.0
55	21386.0
56	17628.5
57	13871.0
58	12404.0
59	10937.0
60	10158.5
61	9380.0
62	7732.0
63	6084.0
64	5008.5
65	3933.0
66	3237.0
67	2541.0
68	2080.5
69	1620.0
70	1282.0
71	944.0
72	827.5
73	711.0
74	689.0
75	472.5
76	278.0
77	246.0
78	214.0
79	165.0
80	116.0
81	86.5
82	57.0
83	55.0
84	53.0
85	41.5
86	30.0
87	26.0
88	22.0
89	15.0
90	8.0
91	7.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	448875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.254118461070547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29370001321139	20.793175379934432
2	9.644803669332498	5.257212434755116
3	3.800549523707412	3.107418808088611
4	2.0443782363968914	2.228709065359815
5	1.2612374592088458	1.7186957560408758
6	0.8598680747296007	1.406096782174392
7	0.6922556831361582	1.3206772875479518
8	0.5255432173239236	1.1458573681086686
9	0.39579756264304167	0.9708402292988803
>10	3.333070094772203	19.894136240061822
>50	0.6832013290404841	13.008298120207392
>100	0.45081433851349256	19.50942589816704
>500	0.007390398992024468	1.3562199846616223
>1k	0.004926932661349645	3.3545183746028147
>5k	0.0016423108871165485	2.3178829011782875
>10k+	8.211554435582743E-4	2.6108353698122913
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11666	2.598941798941799	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	5194	1.1571150097465888	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT	5163	1.1502088554720133	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	3735	0.8320802005012531	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	3396	0.7565580618212198	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2956	0.6585352269562795	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2557	0.5696463380673907	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1198	0.2668894458368143	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTT	1147	0.2555277081592871	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT	842	0.1875800612642718	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	840	0.18713450292397663	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTAT	767	0.17087162350320245	No Hit
AACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	622	0.13856864383180173	No Hit
GTGCGGGATGGTAATTGTTACTATATTCTTTTTATCTTTAGTAGACACTTT	609	0.13567251461988306	No Hit
AAAGTTGGGGCTTTGAATTCTGGTTTGTCTTTGTTTTTCCTATGTTTCCAT	606	0.13500417710944027	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGTC	598	0.13322194374825955	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	593	0.1321080478975216	No Hit
CGAAATGGGAACTTGGATATTTCAAACTCTATTTCCCCTCTTTTGAGAAAG	583	0.12988025619604565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.227791701475912E-4	0.0	0.0	0.9203007518796993	0.0
2	2.227791701475912E-4	0.0	0.0	1.1185742133110554	0.0
3	2.227791701475912E-4	0.0	0.0	1.4703425229741018	0.0
4	2.227791701475912E-4	0.0	0.0	3.2933444722918406	0.0
5	2.227791701475912E-4	0.0	0.0	3.481815650236703	0.0
6	2.227791701475912E-4	0.0	0.0	4.548705096073517	0.0
7	2.227791701475912E-4	0.0	0.0	5.39192425508215	0.0
8	2.227791701475912E-4	0.0	0.0	6.051796157059315	0.0
9	2.227791701475912E-4	0.0	0.0	8.258646616541354	0.0
10	2.227791701475912E-4	0.0	0.0	9.698468393205236	0.0
11	2.227791701475912E-4	0.0	0.0	12.173990531885268	0.0
12	2.227791701475912E-4	0.0	0.0	12.861487050960735	0.0
13	2.227791701475912E-4	0.0	0.0	13.194987468671679	0.0
14	2.227791701475912E-4	0.0	0.0	13.635421888053466	0.0
15	2.227791701475912E-4	0.0	0.0	13.915900863269284	0.0
16	2.227791701475912E-4	0.0	0.0	14.4416597048176	0.0
17	2.227791701475912E-4	0.0	0.0	15.101308827624617	0.0
18	2.227791701475912E-4	0.0	0.0	16.20629351155667	0.0
19	2.227791701475912E-4	0.0	0.0	16.608187134502923	0.0
20	2.227791701475912E-4	0.0	0.0	17.0359231411863	0.0
21	2.227791701475912E-4	0.0	0.0	17.46031746031746	0.0
22	2.227791701475912E-4	0.0	0.0	17.939961013645224	0.0
23	2.227791701475912E-4	0.0	0.0	18.39242550821498	0.0
24	2.227791701475912E-4	0.0	0.0	18.725480367585632	0.0
25	2.227791701475912E-4	0.0	0.0	19.02088554720134	0.0
26	2.227791701475912E-4	0.0	0.0	19.281759955444166	0.0
27	2.227791701475912E-4	0.0	0.0	19.596324143692566	0.0
28	2.227791701475912E-4	0.0	0.0	19.90754664438875	0.0
29	2.227791701475912E-4	0.0	0.0	20.236145920356446	0.0
30	2.227791701475912E-4	0.0	0.0	20.686382623224727	0.0
31	2.227791701475912E-4	0.0	0.0	20.999832915622388	0.0
32	2.227791701475912E-4	0.0	0.0	21.3130604288499	0.0
33	2.227791701475912E-4	0.0	0.0	21.597772208298522	0.0
34	2.227791701475912E-4	0.0	0.0	21.89228627123364	0.0
35	2.227791701475912E-4	0.0	0.0	22.235588972431078	0.0
36	2.227791701475912E-4	0.0	0.0	22.543915343915344	0.0
37	2.227791701475912E-4	0.0	0.0	22.839766081871346	0.0
38	2.227791701475912E-4	0.0	0.0	23.16324143692565	0.0
39	2.227791701475912E-4	0.0	0.0	23.45686438318017	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATC	30	2.1625347E-6	45.000004	44
CTATGCG	30	2.1625347E-6	45.000004	1
CGAACCG	30	2.1625347E-6	45.000004	22
ACGCCAT	30	2.1625347E-6	45.000004	22
CTACTAG	60	0.0	45.000004	1
AATCACG	60	0.0	45.000004	1
AGTCACG	35	1.2097189E-7	45.000004	1
GGTCGGA	30	2.1625347E-6	45.000004	8
GCAACTT	35	1.2097189E-7	45.000004	9
TTTGTCG	30	2.1625347E-6	45.000004	21
GAACTTC	35	1.2097189E-7	45.000004	9
CGCATAG	35	1.2097189E-7	45.000004	1
GGCAACG	30	2.1625347E-6	45.000004	1
GGCAACC	95	0.0	45.000004	8
TCGGGCT	30	2.1625347E-6	45.000004	5
ACTTCGT	30	2.1625347E-6	45.000004	23
GCGCCAT	30	2.1625347E-6	45.000004	37
CCAGATA	35	1.2097189E-7	45.000004	18
GCCATAC	30	2.1625347E-6	45.000004	39
GCCGCGA	35	1.2097189E-7	45.000004	27
>>END_MODULE