##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443684
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45873414412059	31.0	31.0	34.0	30.0	34.0
2	31.669151918933295	31.0	31.0	34.0	30.0	34.0
3	31.840548678789407	31.0	31.0	34.0	30.0	34.0
4	35.57882637192236	37.0	35.0	37.0	33.0	37.0
5	35.33969672108979	37.0	35.0	37.0	33.0	37.0
6	32.4418910756304	37.0	35.0	37.0	25.0	37.0
7	34.01123547389584	37.0	35.0	37.0	26.0	37.0
8	35.1465164396282	37.0	35.0	37.0	32.0	37.0
9	37.02802895754636	39.0	37.0	39.0	34.0	39.0
10	36.51958150395326	38.0	35.0	39.0	32.0	39.0
11	36.625783215081	39.0	35.0	39.0	32.0	39.0
12	36.55057428259752	38.0	35.0	39.0	32.0	39.0
13	36.60604844889606	39.0	35.0	39.0	32.0	39.0
14	37.67761514952083	39.0	37.0	41.0	32.0	41.0
15	37.80001983393586	39.0	37.0	41.0	33.0	41.0
16	37.633065424942075	39.0	36.0	41.0	32.0	41.0
17	37.63918689878382	39.0	36.0	41.0	32.0	41.0
18	37.44945276367866	39.0	36.0	40.0	32.0	41.0
19	37.34365900054994	39.0	36.0	40.0	32.0	41.0
20	37.33756682684072	39.0	36.0	40.0	32.0	41.0
21	37.32604511318866	39.0	36.0	40.0	32.0	41.0
22	37.243644125098044	39.0	35.0	40.0	32.0	41.0
23	37.06057915092724	39.0	35.0	40.0	31.0	41.0
24	36.97414826768601	39.0	35.0	40.0	31.0	41.0
25	37.01446074233013	39.0	35.0	40.0	31.0	41.0
26	36.94718313033601	39.0	35.0	40.0	31.0	41.0
27	36.89178784900965	39.0	35.0	40.0	31.0	41.0
28	36.807367405631034	39.0	35.0	40.0	31.0	41.0
29	36.6187331524238	39.0	35.0	40.0	31.0	41.0
30	36.66521217803662	39.0	35.0	40.0	31.0	41.0
31	36.20720828337285	38.0	35.0	40.0	30.0	41.0
32	36.372199583487344	38.0	35.0	40.0	30.0	41.0
33	36.27343109059601	38.0	35.0	40.0	30.0	41.0
34	36.256606052956606	38.0	35.0	40.0	30.0	41.0
35	36.24465385274204	38.0	35.0	40.0	30.0	41.0
36	36.09707133906113	38.0	35.0	40.0	30.0	41.0
37	36.10687110646316	38.0	35.0	40.0	30.0	41.0
38	35.93406568638941	38.0	35.0	40.0	29.0	41.0
39	35.86005129777048	38.0	35.0	40.0	29.0	41.0
40	35.67678122267199	38.0	35.0	40.0	28.0	41.0
41	35.6690166875524	38.0	35.0	40.0	28.0	41.0
42	35.61407668520839	38.0	35.0	40.0	28.0	41.0
43	35.586237051595276	38.0	35.0	40.0	28.0	41.0
44	35.46408705294759	38.0	34.0	40.0	27.0	41.0
45	35.254742113756635	38.0	34.0	40.0	26.0	41.0
46	34.93593413330208	38.0	33.0	40.0	25.0	41.0
47	34.99416927362718	38.0	34.0	40.0	26.0	41.0
48	35.03510832033609	38.0	34.0	40.0	26.0	41.0
49	34.92209545532406	37.0	34.0	40.0	26.0	41.0
50	34.788577005255995	37.0	33.0	40.0	26.0	41.0
51	33.66724064875001	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	0.0
12	3.0
13	5.0
14	7.0
15	16.0
16	42.0
17	102.0
18	214.0
19	444.0
20	912.0
21	1380.0
22	1977.0
23	2507.0
24	2941.0
25	3232.0
26	3731.0
27	4504.0
28	5728.0
29	7288.0
30	9644.0
31	13045.0
32	17464.0
33	23120.0
34	32277.0
35	45097.0
36	47558.0
37	61115.0
38	80566.0
39	78743.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.96075585326494	38.1981320038586	20.797234067489477	23.043878075386985
2	19.872251422183357	38.04689824289359	20.76951163440647	21.311338700516583
3	18.006283751498813	33.660668403638624	24.75725967129759	23.57578817356497
4	21.66970185988226	34.21669476474247	19.31306966219201	24.800533713183256
5	16.154289990173186	40.95662678843502	17.27873892229605	25.610344299095754
6	16.0810396588563	43.98490817789237	20.736605331722576	19.197446831528744
7	77.87051144508254	13.242307588283552	4.729492161087621	4.1576888055462895
8	79.34273041173448	9.768889570054364	3.1874036476411143	7.700976370570045
9	75.72709405793312	13.706601996015182	6.519054101567782	4.047249844483912
10	29.097285455414212	46.88269128478827	11.962342568134078	12.057680691663435
11	19.527411400906953	30.143750957888948	32.57566195760947	17.75317568359463
12	22.77882456883728	28.845304315684135	33.023277828364336	15.352593287114253
13	22.3778635244904	31.61754762398464	26.145409796161236	19.859179055363725
14	16.586579637760206	32.97684838759117	29.552789823387815	20.883782151260807
15	16.28884521416143	35.191713020978895	26.42376105516539	22.09568070969429
16	17.820565988406162	35.16894907186195	28.088684739589436	18.921800200142442
17	17.347932312186153	34.016326935386445	27.987035818285083	20.648704934142316
18	18.214765463708403	33.54189017408786	25.67638229009836	22.566962072105373
19	17.706520857186646	35.98709892626283	27.48081968247672	18.8255605340738
20	19.84633207417892	36.29407416088928	25.98989370813462	17.869700056797182
21	18.69325916643377	34.08980265233815	27.23672703996538	19.9802111412627
22	20.264647812407027	34.16823685325593	25.69193389890102	19.875181435436033
23	18.776877236952423	35.61859341333021	23.39007041047232	22.21445893924505
24	14.768619107292578	38.65791869889381	26.295516628952136	20.277945564861476
25	15.59307975946845	36.22690924171257	26.653203631413348	21.52680736740563
26	20.659298058978912	36.16988667610281	22.649002443180283	20.521812821737996
27	15.757836658522734	35.4376087485688	27.015849117840624	21.78870547506784
28	18.1487274727058	34.62374122122952	28.20791374040984	19.019617565654837
29	19.141325808458273	35.22281623858422	24.692348608469093	20.94350934448842
30	19.12374572894222	34.35034844619143	24.55103181543621	21.974874009430135
31	21.362501239620993	37.45435940894871	22.197329630998638	18.98580972043166
32	19.06446930698425	31.654510868095308	24.66913388808251	24.61188593683793
33	18.937126423310282	35.13784585425663	24.892040280920654	21.032987441512425
34	18.577636335770503	33.580431117642284	26.24998873071826	21.59194381586895
35	19.76429170310401	31.76923215621929	25.684946944221565	22.781529196455136
36	18.916165559271914	35.68395524742835	25.704330108816187	19.695549084483552
37	18.384706232363573	34.72110781547227	24.220841860423185	22.67334409174097
38	18.203496181967346	36.21406226052776	26.23646559262899	19.3459759648759
39	19.042156129136952	33.85224619323663	24.28710523706061	22.81849244056581
40	21.75624994365359	35.58410941120257	22.881375032680918	19.778265612462924
41	19.055904652861045	32.022790995393116	28.63028642006473	20.291017931681107
42	22.818041669296164	30.691212664869592	24.91390268749831	21.576842978335932
43	20.73480224664401	32.21076261483398	26.15983447678979	20.894600661732223
44	20.421290828607745	31.561877372183805	23.372715716591088	24.64411608261736
45	21.898693664860577	35.11891346093166	23.706962613030896	19.275430261176872
46	17.493531432280633	32.810063017823495	28.20723758350538	21.489167966390493
47	19.37099377034105	34.28340891264954	25.667141479070686	20.678455837938714
48	18.551491602131247	31.863668737209366	28.942220138657245	20.642619522002146
49	20.457577915813957	31.46856771936784	25.987414466151588	22.08643989866662
50	18.95042417576473	30.478448625598396	25.720332488888488	24.85079470974838
51	17.54694782773325	29.4993734279352	29.173465799983774	23.780212944347777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13102.0
1	10358.0
2	7614.0
3	4647.5
4	1681.0
5	1360.5
6	1040.0
7	1121.5
8	1203.0
9	1332.0
10	1461.0
11	1691.0
12	1921.0
13	2251.5
14	2582.0
15	2606.5
16	2631.0
17	2539.5
18	2448.0
19	2676.5
20	2905.0
21	2821.5
22	2738.0
23	3113.0
24	3488.0
25	4151.5
26	5264.0
27	5713.0
28	5841.0
29	5969.0
30	7619.5
31	9270.0
32	12752.5
33	16235.0
34	16287.0
35	16339.0
36	17934.5
37	19530.0
38	19457.0
39	19384.0
40	19894.0
41	20404.0
42	21876.0
43	23348.0
44	31588.0
45	39828.0
46	40127.0
47	40426.0
48	40959.5
49	41493.0
50	38914.5
51	36336.0
52	32108.5
53	27881.0
54	24528.0
55	21175.0
56	17449.0
57	13723.0
58	12304.0
59	10885.0
60	10069.0
61	9253.0
62	7510.5
63	5768.0
64	4905.5
65	4043.0
66	3197.0
67	2351.0
68	2016.5
69	1682.0
70	1305.0
71	928.0
72	810.0
73	692.0
74	649.5
75	448.0
76	289.0
77	259.0
78	229.0
79	169.0
80	109.0
81	81.0
82	53.0
83	49.0
84	45.0
85	38.0
86	31.0
87	27.5
88	24.0
89	15.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.771332177361828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40003543316655	21.21730762376682
2	9.462612468777763	5.255787082721463
3	3.7117613752120735	3.0924167434234753
4	2.0837942782796905	2.31478972365565
5	1.2936756000254388	1.796354740902717
6	0.90699339914391	1.511304898218003
7	0.6623789490392487	1.287660207474256
8	0.5347951254494913	1.188157846055337
9	0.4129508254591684	1.0321375092068101
>10	3.4409779732891588	20.77374403040419
>50	0.6541782878664832	12.708325166154472
>100	0.42194499567388166	18.626810727175858
>500	0.006541782878664832	1.1922357913959896
>1k	0.006541782878664832	5.382320093690733
>5k	0.0	0.0
>10k+	8.17722859833104E-4	2.620647815754232
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11540	2.600950225836406	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	4768	1.0746387068273817	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT	4635	1.044662417396165	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	3348	0.7545911053813075	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	3112	0.7014000955635091	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2932	0.660830681295697	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2676	0.6031319587814751	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1168	0.2632504214711371	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTT	1062	0.23935954418009214	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT	804	0.18121005039622795	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	773	0.17422309571677141	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTAT	692	0.1559668592962559	No Hit
AACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	636	0.14334526374626988	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGTC	613	0.13816139414538275	No Hit
CGAAATGGGAACTTGGATATTTCAAACTCTATTTCCCCTCTTTTGAGAAAG	602	0.1356821521623498	No Hit
AAAGTTGGGGCTTTGAATTCTGGTTTGTCTTTGTTTTTCCTATGTTTCCAT	583	0.13139982510074738	No Hit
GTGCGGGATGGTAATTGTTACTATATTCTTTTTATCTTTAGTAGACACTTT	547	0.12328594224718493	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	494	0.11134050360166244	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2538563482117903E-4	0.0	0.0	0.8355045482821107	0.0
2	2.2538563482117903E-4	0.0	0.0	1.0200953832006563	0.0
3	2.2538563482117903E-4	0.0	0.0	1.3719223591565168	0.0
4	2.2538563482117903E-4	0.0	0.0	3.034817572867176	0.0
5	2.2538563482117903E-4	0.0	0.0	3.2205353359598274	0.0
6	2.2538563482117903E-4	0.0	0.0	4.238827634081914	0.0
7	2.2538563482117903E-4	0.0	0.0	5.047962063089947	0.0
8	2.2538563482117903E-4	0.0	0.0	5.681971853841923	0.0
9	2.2538563482117903E-4	0.0	0.0	7.755068922927128	0.0
10	2.2538563482117903E-4	0.0	0.0	9.12203279811758	0.0
11	2.2538563482117903E-4	0.0	0.0	11.577383903859504	0.0
12	2.2538563482117903E-4	0.0	0.0	12.269993959664987	0.0
13	2.2538563482117903E-4	0.0	0.0	12.587336933493207	0.0
14	2.2538563482117903E-4	0.0	0.0	13.015569639653448	0.0
15	2.2538563482117903E-4	0.0	0.0	13.29324474175314	0.0
16	2.2538563482117903E-4	0.0	0.0	13.80396859025793	0.0
17	2.2538563482117903E-4	0.0	0.0	14.448796891481324	0.0
18	2.2538563482117903E-4	0.0	0.0	15.573245823604188	0.0
19	2.2538563482117903E-4	0.0	0.0	15.980743051360879	0.0
20	2.2538563482117903E-4	0.0	0.0	16.39770647578006	0.0
21	2.2538563482117903E-4	0.0	0.0	16.809260644963533	0.0
22	2.2538563482117903E-4	0.0	0.0	17.281443549913902	0.0
23	2.2538563482117903E-4	0.0	0.0	17.721170923450025	0.0
24	2.2538563482117903E-4	0.0	0.0	18.043246995609486	0.0
25	2.2538563482117903E-4	0.0	0.0	18.314385914299365	0.0
26	2.2538563482117903E-4	0.0	0.0	18.585750218624067	0.0
27	2.2538563482117903E-4	0.0	0.0	18.89385238142462	0.0
28	2.2538563482117903E-4	0.0	0.0	19.199024530972494	0.0
29	2.2538563482117903E-4	0.0	0.0	19.50780285067751	0.0
30	2.2538563482117903E-4	0.0	0.0	19.93941634136007	0.0
31	2.2538563482117903E-4	0.0	0.0	20.242334634559732	0.0
32	2.2538563482117903E-4	0.0	0.0	20.549535254821	0.0
33	2.2538563482117903E-4	0.0	0.0	20.836901939218002	0.0
34	2.2538563482117903E-4	0.0	0.0	21.13080480702482	0.0
35	2.2538563482117903E-4	0.0	0.0	21.47654637084051	0.0
36	2.2538563482117903E-4	0.0	0.0	21.789156246337484	0.0
37	2.2538563482117903E-4	0.0	0.0	22.091849153902327	0.0
38	2.2538563482117903E-4	0.0	0.0	22.406262114477872	0.0
39	2.2538563482117903E-4	0.0	0.0	22.7055742375204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATC	20	7.028598E-4	45.000004	44
TTCTACG	40	6.7993824E-9	45.000004	1
GTCGCTT	20	7.028598E-4	45.000004	40
TTGGCAT	20	7.028598E-4	45.000004	16
ACCGGCA	20	7.028598E-4	45.000004	29
ATTAGTT	20	7.028598E-4	45.000004	12
ACGCCCT	20	7.028598E-4	45.000004	25
CGTATAG	20	7.028598E-4	45.000004	1
AGTCACG	20	7.028598E-4	45.000004	1
CGAGTCG	20	7.028598E-4	45.000004	28
CGAGTCC	20	7.028598E-4	45.000004	19
GCGGTTG	20	7.028598E-4	45.000004	36
CGCGGAG	20	7.028598E-4	45.000004	4
CGGTTGG	20	7.028598E-4	45.000004	15
GGCCGTA	20	7.028598E-4	45.000004	42
CGGCAAT	20	7.028598E-4	45.000004	31
CAACCGT	40	6.7993824E-9	45.000004	32
CGCGCGA	20	7.028598E-4	45.000004	35
GTTAGTC	20	7.028598E-4	45.000004	41
GTCTATC	20	7.028598E-4	45.000004	45
>>END_MODULE