FastQCFastQC Report
Sat 18 Jun 2016
SRR3553372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220881
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120880.9901046866975569No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT27360.22410046515589974Illumina PCR Primer Index 8 (95% over 24bp)
ACGTTAGGGGAAAGGTATTTTTTTCCAGATAAACTATAATGTAAACATGCA25080.20542542639290806No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24650.20190337960865964No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22100.18101682309741898No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG14280.11696471646294765No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12410.10164790835470452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT302.1656124E-645.00000441
GTCGAAC302.1656124E-645.00000436
TAGCGGA351.2120108E-745.0000043
GCGTTAT351.2120108E-745.00000434
TCTAGCG950.045.0000041
CGCGTTA351.2120108E-745.00000433
CGTCCAA351.2120108E-745.00000442
ATCGCGT302.1656124E-645.00000410
AACCGCG207.0334587E-445.01
ACGCATA207.0334587E-445.027
CTATACG207.0334587E-445.01
CATTACG207.0334587E-445.01
ATATCGT207.0334587E-445.019
ATATCCG253.8909486E-545.01
GTTCGTA207.0334587E-445.030
TGCGATC207.0334587E-445.022
CGTAGAC207.0334587E-445.022
CGAATTG406.8157533E-945.01
CGTACGG551.8189894E-1245.02
TACTCGA253.8909486E-545.044