SRR3553371_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1214952
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11758	0.9677748585952366	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	2507	0.20634560048462822	Illumina PCR Primer Index 8 (95% over 24bp)
ACGTTAGGGGAAAGGTATTTTTTTCCAGATAAACTATAATGTAAACATGCA	2497	0.20552252270048527	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2437	0.2005840559956278	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2244	0.1846986547616696	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1400	0.11523088978000777	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	45	3.8562575E-10	45.0	1
CGAGTAT	20	7.0334465E-4	45.0	22
ACGCATA	35	1.2120108E-7	45.0	27
AACCCGT	55	1.8189894E-12	45.0	16
TCTTACG	50	2.1827873E-11	45.0	1
ATTCGCC	20	7.0334465E-4	45.0	29
CCGCTAA	45	3.8562575E-10	45.0	14
ACGATAG	35	1.2120108E-7	45.0	1
ATATCCG	20	7.0334465E-4	45.0	1
CCGTAGA	25	3.8909384E-5	45.0	38
TATCTCG	25	3.8909384E-5	45.0	1
CGCTAAA	25	3.8909384E-5	45.0	40
TATTCGC	20	7.0334465E-4	45.0	28
TATTACG	20	7.0334465E-4	45.0	1
CGGTGTA	20	7.0334465E-4	45.0	14
CGTCGAT	25	3.8909384E-5	45.0	30
ACCGTAC	20	7.0334465E-4	45.0	18
AGCTCGA	25	3.8909384E-5	45.0	30
TTCGCAC	20	7.0334465E-4	45.0	15
TCGCGAC	20	7.0334465E-4	45.0	30