##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1214952
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68796545048693	31.0	31.0	34.0	30.0	34.0
2	31.89550533683635	33.0	31.0	34.0	30.0	34.0
3	32.0676076091895	33.0	31.0	34.0	30.0	34.0
4	35.79073494261502	37.0	35.0	37.0	35.0	37.0
5	35.55028182183329	37.0	35.0	37.0	33.0	37.0
6	32.761222665586786	37.0	35.0	37.0	26.0	37.0
7	34.27667759713964	37.0	35.0	37.0	28.0	37.0
8	35.38956024600149	37.0	35.0	37.0	32.0	37.0
9	37.247979344039926	39.0	37.0	39.0	34.0	39.0
10	36.74710852774431	39.0	37.0	39.0	32.0	39.0
11	36.786690338383735	39.0	37.0	39.0	32.0	39.0
12	36.71418130098967	39.0	35.0	39.0	32.0	39.0
13	36.69577810481402	39.0	35.0	39.0	32.0	39.0
14	37.86110397777031	40.0	37.0	41.0	33.0	41.0
15	37.914600741428465	40.0	37.0	41.0	33.0	41.0
16	37.82945663697002	40.0	37.0	41.0	33.0	41.0
17	37.854796732710426	40.0	37.0	41.0	33.0	41.0
18	37.76569527026582	39.0	37.0	41.0	33.0	41.0
19	37.79157530503263	40.0	36.0	41.0	33.0	41.0
20	37.69422824934647	39.0	36.0	41.0	33.0	41.0
21	37.653048021650235	39.0	36.0	41.0	33.0	41.0
22	37.59618486985494	39.0	36.0	41.0	33.0	41.0
23	37.46558794092277	39.0	36.0	41.0	32.0	41.0
24	37.32746149642126	39.0	36.0	41.0	32.0	41.0
25	37.350520020544025	39.0	36.0	41.0	32.0	41.0
26	37.289547241372496	39.0	36.0	41.0	32.0	41.0
27	37.214997794151536	39.0	35.0	41.0	32.0	41.0
28	37.176253876696364	39.0	35.0	41.0	32.0	41.0
29	36.969489329619606	39.0	35.0	40.0	31.0	41.0
30	37.00604632940232	39.0	35.0	40.0	31.0	41.0
31	36.664563702928184	39.0	35.0	40.0	30.0	41.0
32	36.75479195885928	39.0	35.0	40.0	31.0	41.0
33	36.76303096747855	39.0	35.0	40.0	31.0	41.0
34	36.694067749178565	39.0	35.0	40.0	31.0	41.0
35	36.64913593294221	39.0	35.0	40.0	31.0	41.0
36	36.579044275000165	39.0	35.0	40.0	30.0	41.0
37	36.48487018417188	39.0	35.0	40.0	30.0	41.0
38	36.375339108047065	38.0	35.0	40.0	30.0	41.0
39	36.28137407897596	38.0	35.0	40.0	30.0	41.0
40	36.197067044623985	38.0	35.0	40.0	30.0	41.0
41	36.170608386174926	38.0	35.0	40.0	30.0	41.0
42	36.08153161606384	38.0	35.0	40.0	30.0	41.0
43	35.98561836187767	38.0	35.0	40.0	30.0	41.0
44	35.803119794033016	38.0	35.0	40.0	29.0	41.0
45	35.63209904588823	38.0	34.0	40.0	28.0	41.0
46	35.37774167209898	38.0	34.0	40.0	27.0	41.0
47	35.35407489349373	38.0	34.0	40.0	28.0	41.0
48	35.31833520995068	37.0	34.0	40.0	28.0	41.0
49	35.224687065826465	37.0	34.0	40.0	27.0	41.0
50	35.10825777479275	37.0	34.0	40.0	27.0	41.0
51	33.95452166011497	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	3.0
10	11.0
11	19.0
12	11.0
13	21.0
14	44.0
15	77.0
16	149.0
17	236.0
18	517.0
19	896.0
20	1533.0
21	2376.0
22	3421.0
23	4594.0
24	5919.0
25	7211.0
26	8753.0
27	11354.0
28	14241.0
29	18366.0
30	24663.0
31	31942.0
32	42447.0
33	58125.0
34	85767.0
35	107618.0
36	128121.0
37	176805.0
38	237951.0
39	241710.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.70131165675681	32.67363648934279	20.326893572750198	26.2981582811502
2	23.40166525097288	30.81241069606042	20.365825151940157	25.420098901026545
3	21.029143538180932	31.930479558040155	20.698183961177065	26.342192942601848
4	21.25409069658719	32.06875662577616	19.591473572618508	27.08567910501814
5	18.854489724696943	34.94360271023053	17.27187576134695	28.93003180372558
6	18.130675121321666	42.37080971100093	17.91173643073965	21.586778736937756
7	86.0043030506555	6.406508240654775	3.905339470201292	3.683849238488434
8	86.57304979949825	6.076783280327124	3.64195457927556	3.708212340899064
9	80.34967636581527	9.435599101857523	5.931756974761143	4.28296755756606
10	36.52778052137039	35.94668760576549	12.786677992216976	14.738853880647138
11	27.002465941041294	29.146089722063095	25.79731544949924	18.054128887396377
12	27.561664987587985	25.560762894336563	28.017485464446334	18.860086653629114
13	25.16593248128321	27.23794849508458	26.019628758996237	21.576490264635968
14	21.238616834245303	28.3962658607089	27.23597310840264	23.12914419664316
15	20.203925751799247	29.90266282124726	28.742946223389893	21.1504652035636
16	22.636861373947283	28.367128907150242	27.536396499614803	21.459613219287675
17	21.776333550625868	29.786197314791035	26.819907288518397	21.6175618460647
18	23.30536515022816	29.19530977355484	25.68010917303729	21.819215903179714
19	22.83604619770987	30.788459132541863	25.623563729266667	20.7519309404816
20	24.066959023895595	29.395976137328883	26.167206605693067	20.369858233082457
21	24.383020893006474	29.665451803857273	25.71681844221006	20.234708860926194
22	23.674515536416255	27.34774707148924	26.296429817803503	22.681307574291
23	23.249148937571196	29.174732828951267	26.564506252098845	21.01161198137869
24	21.301582284732238	29.82463504731051	27.191773831394162	21.682008836563092
25	22.204333998380182	30.22950700933041	25.271286437653508	22.2948725546359
26	23.15251960571282	29.844059683016283	24.670439655229178	22.33298105604172
27	20.963544238784742	29.224199803778255	26.37923144288828	23.433024514548723
28	22.128281611125377	30.39980180286958	26.794638800545208	20.67727778545984
29	21.225447589699016	31.073161738076895	25.27902336882445	22.42236730339964
30	21.698964238916435	30.215350071443154	26.159881213414195	21.925804476226222
31	23.570560812279005	29.69203721628509	24.343430851589197	22.393971119846707
32	22.254294819877657	29.159423582166212	25.75188155581455	22.834400042141585
33	22.30112794579539	29.439599259888453	25.250709493049932	23.00856330126622
34	20.504842989681897	29.185679763480366	27.060657540380195	23.24881970645754
35	21.984407614457197	29.13728278977277	26.62401477589238	22.254294819877657
36	22.12490699221039	31.949657270410682	24.065312868327307	21.86012286905162
37	22.04251690601769	32.02472196432451	24.920326070494966	21.012435059162833
38	21.791231258518856	30.87496460765528	26.593396282322264	20.740407851503598
39	21.784564328467297	29.33292837906353	26.019711066774654	22.862796225694513
40	22.461545805924843	29.508408562642803	26.57158472104248	21.458460910389874
41	20.84000026338489	27.489563373697067	27.969170798517144	23.7012655644009
42	21.23417221421093	28.162347154455485	26.493556947105727	24.109923684227855
43	21.166926759246458	29.01579650883327	26.45083921010871	23.366437521811562
44	22.14194470234215	28.480137486913065	25.589159077889494	23.78875873285529
45	22.717605304571702	28.710681574251495	26.139057345475376	22.432655775701427
46	20.67102239430035	28.87126404993777	27.773195978112714	22.684517577649157
47	21.362819271872468	28.90566870131495	27.866779922169766	21.86473210464282
48	20.43010752688172	28.823525538457485	27.67278048844728	23.07358644621351
49	21.451382441446246	29.073905800393764	26.475449235854587	22.999262522305408
50	21.001405816855314	28.02950240009482	27.381904799531178	23.58718698351869
51	21.033176619323232	26.878757350084616	28.194529495815473	23.893536534776683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13869.0
1	10550.0
2	7231.0
3	4679.0
4	2127.0
5	1811.0
6	1495.0
7	1588.0
8	1681.0
9	2005.5
10	2330.0
11	2523.0
12	2716.0
13	3246.5
14	3777.0
15	3902.5
16	4028.0
17	4246.0
18	4464.0
19	4852.0
20	5240.0
21	5594.0
22	5948.0
23	7389.5
24	8831.0
25	8156.5
26	10564.0
27	13646.0
28	18842.5
29	24039.0
30	27742.0
31	31445.0
32	32183.5
33	32922.0
34	36385.0
35	39848.0
36	45790.5
37	51733.0
38	50922.5
39	50112.0
40	51951.5
41	53791.0
42	56571.0
43	59351.0
44	66076.5
45	72802.0
46	78499.5
47	84197.0
48	85474.0
49	86751.0
50	87265.0
51	87779.0
52	80889.0
53	73999.0
54	68998.0
55	63997.0
56	60770.5
57	57544.0
58	55228.5
59	52913.0
60	52209.5
61	51506.0
62	46796.5
63	42087.0
64	36995.5
65	31904.0
66	28229.5
67	24555.0
68	21278.5
69	18002.0
70	15393.0
71	12784.0
72	11555.0
73	10326.0
74	8731.0
75	5816.5
76	4497.0
77	3304.0
78	2111.0
79	1582.0
80	1053.0
81	738.0
82	423.0
83	356.0
84	289.0
85	193.5
86	98.0
87	65.5
88	33.0
89	31.5
90	30.0
91	22.5
92	15.0
93	11.5
94	8.0
95	6.0
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1214952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.53125065779667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79766451896747	16.320185140334694
2	9.237955669486265	3.9780947817064516
3	3.477096719027941	2.24598723056379
4	1.9252173946498883	1.6580935317982797
5	1.2109011893944046	1.3036108515337521
6	0.8887264157627276	1.1481234734395502
7	0.6603536853031137	0.9952768504742783
8	0.5198350072290127	0.8954158273076332
9	0.4124923544149964	0.7993328649600587
>10	3.7730692127173824	18.029376976065706
>50	1.1765977272510477	18.221072904082398
>100	0.9134930869014204	31.7485744739325
>500	0.0034925394146613354	0.5598128539048459
>1k	0.0027164195447365942	1.114612458371738
>5k	0.0	0.0
>10k+	3.8805993496237055E-4	0.9824297815243551
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11758	0.9677748585952366	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	2507	0.20634560048462822	Illumina PCR Primer Index 8 (95% over 24bp)
ACGTTAGGGGAAAGGTATTTTTTTCCAGATAAACTATAATGTAAACATGCA	2497	0.20552252270048527	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2437	0.2005840559956278	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2244	0.1846986547616696	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1400	0.11523088978000777	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2589402708913603	0.0
2	0.0	0.0	0.0	0.3437995904364946	0.0
3	0.0	0.0	0.0	0.4459435434486301	0.0
4	0.0	0.0	0.0	0.9128755703929045	0.0
5	0.0	0.0	0.0	0.9881871876419809	0.0
6	0.0	0.0	0.0	1.4592346035069699	0.0
7	0.0	0.0	0.0	1.8244342163311802	0.0
8	0.0	0.0	0.0	2.172843042358875	0.0
9	0.0	0.0	0.0	2.7346759378148273	0.0
10	0.0	0.0	0.0	3.538493701808796	0.0
11	0.0	0.0	0.0	4.290210642066518	0.0
12	0.0	0.0	0.0	4.625367915769512	0.0
13	0.0	0.0	0.0	4.772534223574264	0.0
14	0.0	0.0	0.0	4.9244743825270465	0.0
15	0.0	0.0	0.0	5.057648368001369	0.0
16	0.0	0.0	0.0	5.297493234300615	0.0
17	0.0	0.0	0.0	5.648700524794395	0.0
18	0.0	0.0	0.0	6.058181722405494	0.0
19	0.0	0.0	0.0	6.238764988246449	0.0
20	0.0	0.0	0.0	6.448979054316549	0.0
21	0.0	0.0	0.0	6.693186232871751	0.0
22	0.0	0.0	0.0	6.955089583786026	0.0
23	0.0	0.0	0.0	7.222507555854058	0.0
24	0.0	0.0	0.0	7.414284679559357	0.0
25	0.0	0.0	0.0	7.587707168678269	0.0
26	0.0	0.0	0.0	7.73676655538655	0.0
27	0.0	0.0	0.0	7.906567502255233	0.0
28	0.0	0.0	0.0	8.086821536982532	0.0
29	0.0	0.0	0.0	8.273495578426143	0.0
30	0.0	0.0	0.0	8.515398139185745	0.0
31	0.0	0.0	0.0	8.693759095009515	0.0
32	0.0	0.0	0.0	8.883972370924942	0.0
33	0.0	0.0	0.0	9.080111806886197	0.0
34	0.0	0.0	0.0	9.261353534954468	0.0
35	0.0	0.0	0.0	9.46934529100738	0.0
36	0.0	0.0	0.0	9.663756263621938	0.0
37	0.0	0.0	0.0	9.857344158452351	0.0
38	8.230777841429126E-5	0.0	0.0	10.064924375613193	0.0
39	8.230777841429126E-5	0.0	0.0	10.273245362779765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	45	3.8562575E-10	45.0	1
CGAGTAT	20	7.0334465E-4	45.0	22
ACGCATA	35	1.2120108E-7	45.0	27
AACCCGT	55	1.8189894E-12	45.0	16
TCTTACG	50	2.1827873E-11	45.0	1
ATTCGCC	20	7.0334465E-4	45.0	29
CCGCTAA	45	3.8562575E-10	45.0	14
ACGATAG	35	1.2120108E-7	45.0	1
ATATCCG	20	7.0334465E-4	45.0	1
CCGTAGA	25	3.8909384E-5	45.0	38
TATCTCG	25	3.8909384E-5	45.0	1
CGCTAAA	25	3.8909384E-5	45.0	40
TATTCGC	20	7.0334465E-4	45.0	28
TATTACG	20	7.0334465E-4	45.0	1
CGGTGTA	20	7.0334465E-4	45.0	14
CGTCGAT	25	3.8909384E-5	45.0	30
ACCGTAC	20	7.0334465E-4	45.0	18
AGCTCGA	25	3.8909384E-5	45.0	30
TTCGCAC	20	7.0334465E-4	45.0	15
TCGCGAC	20	7.0334465E-4	45.0	30
>>END_MODULE