SRR3553370_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422398
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22983	5.441076899038348	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5318	1.2590021733057448	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4173	0.9879308140663545	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGT	3000	0.7102306355617214	No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	2618	0.6197946013001956	RNA PCR Primer, Index 30 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2356	0.5577677924611385	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	2212	0.5236767219541759	No Hit
AATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT	2197	0.5201255687763673	RNA PCR Primer, Index 23 (95% over 23bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	742	0.17566371052893243	No Hit
AAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT	683	0.16169584136288526	RNA PCR Primer, Index 23 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT	675	0.1598018930013873	RNA PCR Primer, Index 23 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT	608	0.14394007547384222	RNA PCR Primer, Index 23 (95% over 21bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	593	0.14038892229603359	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT	580	0.13731125620859946	No Hit
TTTACTGGGGTTATTTTTTTGGTGGGATTAGCATGTCACTAAAGCGGGCCT	503	0.11908200322918196	No Hit
TGCGGAGGGAGAGCTGTGTGTGTATGTGTGTGTGTGTGTCTGTGTGTCTGT	463	0.10961226142169234	No Hit
AACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG	452	0.1070080824246327	RNA PCR Primer, Index 30 (95% over 22bp)
ACAACGATTACTTTAAATGTCTTCTCTGCCTTAGAATTGATATTTTTATTT	435	0.10298344215644961	No Hit
TTGAAAGGGACCGATGTCTTGAGGGAAGAAATTTTTTTTGGCCTATGGAAT	425	0.1006160067045772	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGATC	55	1.8189894E-12	45.000004	11
TGGGCAT	110	0.0	45.000004	6
CGATAGG	55	1.8189894E-12	45.000004	2
GCATAAC	20	7.0282136E-4	45.0	33
GTTGATA	45	3.8380676E-10	45.0	39
CTTCGTG	20	7.0282136E-4	45.0	41
TATTTCG	20	7.0282136E-4	45.0	19
ATTTACG	25	3.8866005E-5	45.0	1
ACAACGA	50	2.1827873E-11	45.0	1
TTGATCG	35	1.2094824E-7	45.0	26
CCTTACG	20	7.0282136E-4	45.0	1
TCGTTAA	20	7.0282136E-4	45.0	12
ATCTCCC	20	7.0282136E-4	45.0	35
CGAACTG	45	3.8380676E-10	45.0	27
ACACGTC	20	7.0282136E-4	45.0	38
GCAAGTC	20	7.0282136E-4	45.0	3
AGATCCG	25	3.8866005E-5	45.0	17
ATTAGTA	20	7.0282136E-4	45.0	13
CATATGC	20	7.0282136E-4	45.0	40
TCACCGA	25	3.8866005E-5	45.0	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content