##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422398
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54428051269182	31.0	31.0	34.0	30.0	34.0
2	31.735065033451864	31.0	31.0	34.0	30.0	34.0
3	31.922653516351875	33.0	31.0	34.0	30.0	34.0
4	35.65463378140995	37.0	35.0	37.0	33.0	37.0
5	35.37192647692461	37.0	35.0	37.0	33.0	37.0
6	34.09549761125763	37.0	35.0	37.0	32.0	37.0
7	34.752908394452625	37.0	35.0	37.0	32.0	37.0
8	35.37789241426333	37.0	35.0	37.0	32.0	37.0
9	37.03399400565343	39.0	37.0	39.0	33.0	39.0
10	36.60795742404083	38.0	35.0	39.0	32.0	39.0
11	36.621385517923855	39.0	35.0	39.0	32.0	39.0
12	36.531214636432935	38.0	35.0	39.0	32.0	39.0
13	36.479720547919264	38.0	35.0	39.0	32.0	39.0
14	37.58638298476792	39.0	36.0	41.0	32.0	41.0
15	37.63031074957741	39.0	36.0	41.0	32.0	41.0
16	37.547433936713716	39.0	36.0	41.0	32.0	41.0
17	37.43388463013556	39.0	36.0	40.0	32.0	41.0
18	37.18069924573506	39.0	36.0	40.0	32.0	41.0
19	36.951822688554394	39.0	35.0	40.0	32.0	41.0
20	36.83223168670306	39.0	35.0	40.0	31.0	41.0
21	36.788971538690994	38.0	35.0	40.0	31.0	41.0
22	36.731348159792425	38.0	35.0	40.0	31.0	41.0
23	36.50003787896723	38.0	35.0	40.0	31.0	41.0
24	36.34659018271867	38.0	35.0	40.0	30.0	41.0
25	36.40197633511522	38.0	35.0	40.0	31.0	41.0
26	36.3125535632271	38.0	35.0	40.0	30.0	41.0
27	36.24775685490935	38.0	35.0	40.0	30.0	41.0
28	36.14852106307322	38.0	35.0	40.0	30.0	41.0
29	35.95924223126057	38.0	35.0	40.0	30.0	41.0
30	35.88794217775652	38.0	35.0	40.0	30.0	41.0
31	35.79636030473629	38.0	35.0	40.0	29.0	41.0
32	35.69885037334457	38.0	35.0	40.0	29.0	41.0
33	35.54948887068594	38.0	34.0	40.0	28.0	41.0
34	35.50181108812068	38.0	34.0	40.0	28.0	41.0
35	35.26266933082069	38.0	34.0	40.0	27.0	41.0
36	35.17692318618933	38.0	34.0	40.0	27.0	41.0
37	35.09783427004863	37.0	34.0	40.0	26.0	41.0
38	35.03588321914403	37.0	34.0	40.0	26.0	41.0
39	34.96396526498705	37.0	34.0	40.0	26.0	41.0
40	34.73930984521707	37.0	33.0	40.0	25.0	41.0
41	34.68116326308363	37.0	33.0	40.0	24.0	41.0
42	34.5985634401678	37.0	33.0	40.0	24.0	41.0
43	34.47206899653881	37.0	33.0	40.0	24.0	41.0
44	34.35640083523123	37.0	33.0	40.0	23.0	41.0
45	34.243043290924675	36.0	33.0	40.0	23.0	41.0
46	34.00173769762167	36.0	33.0	40.0	23.0	41.0
47	33.90904076250361	36.0	33.0	40.0	23.0	41.0
48	33.7622313552621	36.0	33.0	40.0	23.0	41.0
49	33.52628563582214	35.0	33.0	39.0	22.0	41.0
50	33.430430541811276	35.0	32.0	39.0	21.0	41.0
51	32.24282311942765	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	11.0
12	4.0
13	8.0
14	18.0
15	48.0
16	97.0
17	224.0
18	356.0
19	755.0
20	1227.0
21	1801.0
22	2447.0
23	3067.0
24	3724.0
25	4332.0
26	5018.0
27	5878.0
28	7133.0
29	8729.0
30	11024.0
31	13892.0
32	17807.0
33	23113.0
34	33365.0
35	49594.0
36	42342.0
37	53460.0
38	69773.0
39	63130.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.35093916164376	38.23526626546527	19.25648322198495	24.15731135090602
2	19.318746774369195	40.480778791566244	19.66368211970701	20.536792314357548
3	17.97333320707011	38.09085270290106	21.368472388600324	22.56734170142851
4	19.444220853318434	38.75207742460901	17.52044280512692	24.28325891694563
5	15.036529529022392	42.44456649889441	16.61797641087316	25.90092756121004
6	16.409878834653576	43.971325621806926	20.56851594941264	19.050279594126867
7	72.07302117907756	18.537729818796493	5.285536389850331	4.1037126122756264
8	72.7309314911529	15.771618236828772	5.081463453898929	6.415986818119404
9	68.17101406730146	17.940899341379456	8.678071392383487	5.210015198935601
10	30.6608932807447	41.82216771859715	12.269234229328738	15.24770477132941
11	21.18594311526096	36.24662048589245	24.106174745145573	18.461261653701012
12	23.97430859047628	31.151189162827475	28.37797527450414	16.4965269721921
13	22.167955340697638	34.27123234485012	23.63884298694596	19.921969327506286
14	15.887385830425332	35.112855647990756	28.60288164243202	20.39687687915189
15	15.72734719387876	37.860501233433865	26.085114039365713	20.327037533321654
16	19.500329073527812	35.86214896850837	24.7550888025038	19.882433155460017
17	16.658696300645364	36.04325778057662	25.076586536868074	22.221459381909952
18	18.950137074512664	35.42583061472829	24.35688615949886	21.267146151260185
19	19.090762740353885	38.20591006586206	24.030653554230845	18.672673639553217
20	20.394509443700017	37.14387852215209	23.757451503084766	18.70416053106312
21	19.840766291507062	36.82238078778782	23.87037817413908	19.466474746566035
22	19.756722332965595	35.88724378429822	23.446370484708734	20.909663398027455
23	19.685699269409422	35.1417383605036	23.14712664359206	22.025435726494916
24	18.492511801665728	36.93933209911032	23.544619055961437	21.023537043262515
25	18.267842177283036	37.37304627389334	22.864454850638495	21.494656698185125
26	20.019981155213802	36.635826874180275	21.680263637611922	21.663928332994
27	17.28156856803299	36.12919568747958	24.584159962878612	22.005075781608816
28	21.44257311824393	34.463468103542155	23.60806632607162	20.48589245214229
29	20.67978541565064	36.762958157945825	21.54792399585225	21.009332430551282
30	19.404684681272165	36.00348486498516	23.003896798753782	21.5879336549889
31	22.03608918602834	37.0231393141066	20.28276649037164	20.658005009493415
32	22.89712545987434	34.70423628899758	20.7287913295044	21.66984692162368
33	20.558809464059962	36.5778247056094	22.230692380172254	20.632673450158382
34	20.80431252041913	33.73666541981733	24.16725457980388	21.29176747995966
35	22.19518084839417	34.31810756679719	23.40470362075578	20.082007964052863
36	23.245611958389954	34.393628757711916	24.989464912239168	17.37129437165896
37	22.75555281985237	35.412099489107426	23.17340517710785	18.65894251393236
38	22.1778985695955	35.19027078726699	24.185957319873673	18.445873323263843
39	21.93428946159783	34.11095696475835	24.486858365806658	19.467895207837156
40	20.404926159688255	37.910454121468376	22.244423505793115	19.44019621305025
41	18.78204915742972	32.67818502928518	27.876552445797564	20.663213367487536
42	21.45488378259367	33.067391417573	23.609960274433117	21.867764525400215
43	20.05786012244376	32.8261497450272	25.589373055743636	21.5266170767854
44	20.818517133130364	30.483098878309082	25.90424197084267	22.794142017717885
45	21.866817551219466	32.32259622441394	24.684302482492814	21.126283741873777
46	19.309750519652084	33.78567133367108	27.41371881495651	19.49085933172032
47	19.875094105559214	32.97080005113661	25.272846935828298	21.881258907475885
48	20.65161293377336	29.300564870098817	28.92011799298292	21.1277042031449
49	19.006718781812417	32.9151653180176	25.313330082055312	22.764785818114667
50	20.246544727957993	30.967476171762176	25.662526811206494	23.123452289073338
51	20.585798228211306	29.266473799591854	28.66869634799407	21.479031624202765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26011.0
1	19692.5
2	13374.0
3	8229.5
4	3085.0
5	2355.5
6	1626.0
7	1595.0
8	1564.0
9	1575.5
10	1587.0
11	1680.5
12	1774.0
13	1840.5
14	1907.0
15	1872.0
16	1837.0
17	1896.0
18	1955.0
19	1996.5
20	2038.0
21	2227.0
22	2416.0
23	2513.5
24	2611.0
25	2489.5
26	3155.0
27	3942.0
28	4873.5
29	5805.0
30	6326.5
31	6848.0
32	8865.5
33	10883.0
34	10944.5
35	11006.0
36	12169.5
37	13333.0
38	13640.5
39	13948.0
40	14730.5
41	15513.0
42	16769.5
43	18026.0
44	24063.0
45	30100.0
46	30482.5
47	30865.0
48	31756.5
49	32648.0
50	29938.5
51	27229.0
52	25928.5
53	24628.0
54	22397.0
55	20166.0
56	18548.5
57	16931.0
58	17064.0
59	17197.0
60	16099.0
61	15001.0
62	13297.0
63	11593.0
64	11491.5
65	11390.0
66	9118.0
67	6846.0
68	5755.0
69	4664.0
70	4322.5
71	3981.0
72	3159.0
73	2337.0
74	2078.0
75	1292.5
76	766.0
77	559.0
78	352.0
79	288.5
80	225.0
81	153.5
82	82.0
83	71.5
84	61.0
85	44.0
86	27.0
87	19.0
88	11.0
89	9.5
90	8.0
91	8.5
92	9.0
93	6.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	422398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.029446242799658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25157321248298	18.803419719238615
2	9.335757511425784	4.486661665132379
3	3.5065479543711544	2.5278121670208225
4	1.8002459119282137	1.7303564945799543
5	1.0975187129106483	1.3186383456176545
6	0.7266803671490045	1.0477036090862955
7	0.5467594567020869	0.91968288807956
8	0.3958258565500876	0.7609180913184367
9	0.3088641152106791	0.6679650287506403
>10	2.5068970903730903	13.29925337677669
>50	0.7746591886119397	13.58363690572798
>100	0.7336772186337597	29.02681543404934
>500	0.006996921703591713	1.052987661256701
>1k	0.005997361460221468	3.976565629508654
>5k	9.995602433702448E-4	1.277323992372978
>10k+	9.995602433702448E-4	5.520258991483293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22983	5.441076899038348	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5318	1.2590021733057448	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4173	0.9879308140663545	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGT	3000	0.7102306355617214	No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	2618	0.6197946013001956	RNA PCR Primer, Index 30 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2356	0.5577677924611385	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	2212	0.5236767219541759	No Hit
AATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT	2197	0.5201255687763673	RNA PCR Primer, Index 23 (95% over 23bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	742	0.17566371052893243	No Hit
AAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT	683	0.16169584136288526	RNA PCR Primer, Index 23 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT	675	0.1598018930013873	RNA PCR Primer, Index 23 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT	608	0.14394007547384222	RNA PCR Primer, Index 23 (95% over 21bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	593	0.14038892229603359	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT	580	0.13731125620859946	No Hit
TTTACTGGGGTTATTTTTTTGGTGGGATTAGCATGTCACTAAAGCGGGCCT	503	0.11908200322918196	No Hit
TGCGGAGGGAGAGCTGTGTGTGTATGTGTGTGTGTGTGTCTGTGTGTCTGT	463	0.10961226142169234	No Hit
AACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG	452	0.1070080824246327	RNA PCR Primer, Index 30 (95% over 22bp)
ACAACGATTACTTTAAATGTCTTCTCTGCCTTAGAATTGATATTTTTATTT	435	0.10298344215644961	No Hit
TTGAAAGGGACCGATGTCTTGAGGGAAGAAATTTTTTTTGGCCTATGGAAT	425	0.1006160067045772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7481096027916799	0.0
2	0.0	0.0	0.0	0.9630727418216942	0.0
3	0.0	0.0	0.0	1.2845704761859669	0.0
4	0.0	0.0	0.0	2.825297468264528	0.0
5	0.0	0.0	0.0	3.042864786291602	0.0
6	0.0	0.0	0.0	4.174025445196237	0.0
7	0.0	0.0	0.0	5.07199371209144	0.0
8	0.0	0.0	0.0	5.757129531863313	0.0
9	0.0	0.0	0.0	7.336208978262207	0.0
10	0.0	0.0	0.0	8.842844899833805	0.0
11	0.0	0.0	0.0	10.443941495935114	0.0
12	0.0	0.0	0.0	11.060421687602688	0.0
13	0.0	0.0	0.0	11.35445717072524	0.0
14	0.0	0.0	0.0	11.689686030710373	0.0
15	0.0	0.0	0.0	11.928560267804299	0.0
16	0.0	0.0	0.0	12.38405484874455	0.0
17	0.0	0.0	0.0	12.92951197685595	0.0
18	0.0	0.0	0.0	13.93330460844985	0.0
19	0.0	0.0	0.0	14.207453633776675	0.0
20	0.0	0.0	0.0	14.575353102997648	0.0
21	0.0	0.0	0.0	14.871282534481697	0.0
22	0.0	0.0	0.0	15.188282141487413	0.0
23	0.0	0.0	0.0	15.500546877589382	0.0
24	0.0	0.0	0.0	15.706040274811908	0.0
25	0.0	0.0	0.0	15.897565802868385	0.0
26	0.0	0.0	0.0	16.080568563298122	0.0
27	0.0	0.0	0.0	16.29529495878295	0.0
28	0.0	0.0	0.0	16.492975819014294	0.0
29	0.0	0.0	0.0	16.705808266137623	0.0
30	0.0	0.0	0.0	17.009550234612853	0.0
31	0.0	0.0	0.0	17.216464093106502	0.0
32	0.0	0.0	0.0	17.41461844042822	0.0
33	0.0	0.0	0.0	17.619164863469997	0.0
34	0.0	0.0	0.0	17.818029441427278	0.0
35	0.0	0.0	0.0	18.036543733635103	0.0
36	0.0	0.0	0.0	18.23493482450201	0.0
37	0.0	0.0	0.0	18.42101525101918	0.0
38	0.0	0.0	0.0	18.621300290247586	0.0
39	0.0	0.0	0.0	18.828214148741235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGATC	55	1.8189894E-12	45.000004	11
TGGGCAT	110	0.0	45.000004	6
CGATAGG	55	1.8189894E-12	45.000004	2
GCATAAC	20	7.0282136E-4	45.0	33
GTTGATA	45	3.8380676E-10	45.0	39
CTTCGTG	20	7.0282136E-4	45.0	41
TATTTCG	20	7.0282136E-4	45.0	19
ATTTACG	25	3.8866005E-5	45.0	1
ACAACGA	50	2.1827873E-11	45.0	1
TTGATCG	35	1.2094824E-7	45.0	26
CCTTACG	20	7.0282136E-4	45.0	1
TCGTTAA	20	7.0282136E-4	45.0	12
ATCTCCC	20	7.0282136E-4	45.0	35
CGAACTG	45	3.8380676E-10	45.0	27
ACACGTC	20	7.0282136E-4	45.0	38
GCAAGTC	20	7.0282136E-4	45.0	3
AGATCCG	25	3.8866005E-5	45.0	17
ATTAGTA	20	7.0282136E-4	45.0	13
CATATGC	20	7.0282136E-4	45.0	40
TCACCGA	25	3.8866005E-5	45.0	32
>>END_MODULE