FastQCFastQC Report
Sat 18 Jun 2016
SRR3553368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592164
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135590.8516082514112868No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27320.17159036380674353No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT25360.15928007416321435No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23780.1493564733281245No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA22560.14169394610102978No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19320.12134428362907339No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG16520.10375815556688883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC351.21232E-745.00000419
CGGCACG850.045.0000041
GCGCCTA351.21232E-745.00000432
CGTAATG700.045.0000041
GCGCGTA207.0341065E-445.017
CGGCATA207.0341065E-445.039
ATAGCGC253.8914863E-545.036
TACGGTT253.8914863E-545.028
CGTTCGA253.8914863E-545.020
CCGTCGA253.8914863E-545.036
TAGTTCG253.8914863E-545.01
TTGAGCG750.045.01
AATGCGA207.0341065E-445.027
CCGTAGA207.0341065E-445.010
TACCTCG207.0341065E-445.01
TATAGCG406.8175723E-945.01
CGCCGTA207.0341065E-445.034
CGGTACC207.0341065E-445.034
ACATATC551.8189894E-1245.032
CGATAGC253.8914863E-545.045