##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553368_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1592164
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7856056285659	31.0	31.0	34.0	30.0	34.0
2	31.99217480108833	33.0	31.0	34.0	30.0	34.0
3	32.15557819420612	34.0	31.0	34.0	30.0	34.0
4	35.84971020573258	37.0	35.0	37.0	35.0	37.0
5	35.60034456249482	37.0	35.0	37.0	33.0	37.0
6	34.260909680158576	37.0	35.0	37.0	32.0	37.0
7	35.036155823143844	37.0	35.0	37.0	32.0	37.0
8	35.718411545544306	37.0	35.0	37.0	33.0	37.0
9	37.40899932419022	39.0	37.0	39.0	35.0	39.0
10	36.85244736095025	39.0	37.0	39.0	32.0	39.0
11	36.87470888677297	39.0	37.0	39.0	33.0	39.0
12	36.812339683600435	39.0	37.0	39.0	33.0	39.0
13	36.80063423114704	39.0	37.0	39.0	33.0	39.0
14	38.036229308036106	40.0	37.0	41.0	33.0	41.0
15	38.04404006119972	40.0	37.0	41.0	33.0	41.0
16	37.94411568155039	40.0	37.0	41.0	33.0	41.0
17	37.939153881132846	40.0	37.0	41.0	33.0	41.0
18	37.87514602767052	40.0	37.0	41.0	33.0	41.0
19	37.89896015737072	40.0	37.0	41.0	33.0	41.0
20	37.77901020246658	40.0	37.0	41.0	33.0	41.0
21	37.75129823309659	39.0	36.0	41.0	33.0	41.0
22	37.70866254983783	39.0	36.0	41.0	33.0	41.0
23	37.58881057479003	39.0	36.0	41.0	33.0	41.0
24	37.42322084910851	39.0	36.0	41.0	32.0	41.0
25	37.44977590248241	39.0	36.0	41.0	32.0	41.0
26	37.41812024389447	39.0	36.0	41.0	32.0	41.0
27	37.35531955250841	39.0	36.0	41.0	32.0	41.0
28	37.2664310962941	39.0	36.0	41.0	32.0	41.0
29	37.13023469944051	39.0	36.0	41.0	31.0	41.0
30	37.16046085704739	39.0	36.0	41.0	31.0	41.0
31	37.115949110769996	39.0	35.0	41.0	31.0	41.0
32	37.045762245597814	39.0	35.0	41.0	31.0	41.0
33	36.99580508038117	39.0	35.0	41.0	31.0	41.0
34	36.913184822669024	39.0	35.0	40.0	31.0	41.0
35	36.838087031235474	39.0	35.0	40.0	31.0	41.0
36	36.760346924060585	39.0	35.0	40.0	31.0	41.0
37	36.67495433887464	39.0	35.0	40.0	31.0	41.0
38	36.5856061310267	39.0	35.0	40.0	30.0	41.0
39	36.541876339372074	39.0	35.0	40.0	30.0	41.0
40	36.38046394718132	39.0	35.0	40.0	30.0	41.0
41	36.2928178252994	38.0	35.0	40.0	30.0	41.0
42	36.22858323640027	38.0	35.0	40.0	30.0	41.0
43	36.14293314005341	38.0	35.0	40.0	30.0	41.0
44	36.01326622131891	38.0	35.0	40.0	30.0	41.0
45	35.869625867687	38.0	35.0	40.0	29.0	41.0
46	35.71124519835897	38.0	34.0	40.0	29.0	41.0
47	35.609184732226076	38.0	34.0	40.0	28.0	41.0
48	35.514464590331144	38.0	34.0	40.0	28.0	41.0
49	35.340320469499375	37.0	34.0	40.0	28.0	41.0
50	35.23174245869144	37.0	34.0	40.0	27.0	41.0
51	34.07268283920501	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	21.0
10	27.0
11	37.0
12	39.0
13	59.0
14	84.0
15	113.0
16	207.0
17	334.0
18	666.0
19	1137.0
20	1877.0
21	2960.0
22	4031.0
23	5286.0
24	6931.0
25	8821.0
26	10952.0
27	13591.0
28	17323.0
29	22666.0
30	29295.0
31	38499.0
32	51419.0
33	69681.0
34	104211.0
35	130792.0
36	160099.0
37	226003.0
38	332023.0
39	352921.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.91597599242289	32.912187437977494	21.414000065319904	25.75783650427971
2	23.460774141357295	31.215188887576907	20.95424843169422	24.369788539371573
3	21.712901434776818	31.96197125421753	20.192015395398965	26.133111915606683
4	21.501616667629715	32.371288384864876	18.99841976077841	27.128675186726998
5	18.589730706133288	34.49638353837921	16.654942581291877	30.258943174195625
6	18.95200494421429	40.21269165739208	18.892023686002197	21.943279712391437
7	86.32370785924063	6.5358844943108885	3.781645609371899	3.3587620370765827
8	87.38503068779346	5.909064644094453	3.571114533427461	3.1347901346846183
9	80.46576860172696	9.832278584366936	5.614685421853528	4.087267392052577
10	35.711584987476165	38.14682407088717	12.607872053381437	13.53371888825523
11	26.49909180210079	29.78141698970709	25.055521918596323	18.663969289595794
12	27.045329501232285	26.355073974791544	27.19085471094686	19.408741813029312
13	24.965895473079406	26.656676071058005	26.171361744141937	22.206066711720652
14	21.24806238553315	27.696393085134446	26.426674639044723	24.62886989028768
15	20.75565079979198	30.09287987920842	26.310040925432304	22.84142839556729
16	22.760909052082575	29.67345072492532	25.953356563771067	21.612283659221035
17	22.326092035745063	29.62232533834454	25.75720842827749	22.29437419763291
18	23.478234654219037	29.23329506256893	25.08397376149693	22.2044965217151
19	23.0757635519959	29.932218037840325	25.257448353310334	21.73457005685344
20	24.701852321745747	29.146746189462895	25.849472793003734	20.30192869578762
21	25.405360251833354	28.7967194334252	25.395687881399155	20.40223243334229
22	24.60136016139041	27.635030059717465	25.053574882989444	22.710034895902684
23	23.253132215023076	28.927798895088696	25.496180041754492	22.322888848133733
24	21.694750038312634	28.719905738353585	27.124090231910785	22.461253991422993
25	21.963064106461395	30.630575744709716	25.236973075637938	22.16938707319095
26	23.95626329950935	29.799631193771496	24.40012461027884	21.843980896440314
27	22.18132051723315	28.9772912840637	26.073570310596146	22.767817888107004
28	22.011739996633512	30.122901912114582	27.28531734168088	20.580040749571022
29	21.749455458106073	29.314630904856536	25.444929039973267	23.49098459706412
30	22.40033061920757	29.542999339264046	25.517032165028226	22.53963787650016
31	23.945962853072924	29.68149009775375	23.767966114043528	22.604580935129796
32	22.878296456897658	29.100394180498995	25.349461487635693	22.671847874967654
33	22.359882524664545	29.153906255888213	25.232576543622393	23.253634675824852
34	21.819171894352593	29.659130592074685	26.63387691217739	21.887820601395333
35	22.993485595704964	28.006034554229338	26.905519783137915	22.09496006692778
36	23.22436633412136	30.407043495519304	25.269381797352537	21.0992083730068
37	22.043709065146555	31.02023409648755	24.982099833936704	21.953957004429192
38	22.1888574292598	29.22902414575383	26.48948223926681	22.09263618571956
39	22.502895430370238	27.685904215897356	25.912720046427378	23.898480307305025
40	22.851163573601717	29.395150248341253	26.460653550764874	21.293032627292163
41	21.474986245135554	27.486427277592007	28.243698513469717	22.794887963802722
42	22.17742644601938	28.124364073047754	26.099949502689423	23.598259978243448
43	21.740976432076092	28.035679741534164	26.844596410922495	23.378747415467252
44	22.855560105617258	27.477006137558696	25.641579636268624	24.02585412055542
45	22.377343037526284	28.154009260352574	26.897417602709268	22.57123009941187
46	21.25622737356202	28.89444805937077	27.606389794016195	22.24293477305102
47	21.522657213704115	28.614012124379144	27.554636331433198	22.308694330483544
48	21.733941980851217	27.32727281862924	28.2575789931188	22.681206207400745
49	21.50180509043038	29.093485344474562	26.704598270027457	22.700111295067593
50	21.27701668923553	28.015581309463094	27.443152841038987	23.264249160262384
51	21.971543132491377	26.7399589489525	27.35264708911896	23.935850829437168
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15648.0
1	11982.0
2	8316.0
3	5421.5
4	2527.0
5	2183.0
6	1839.0
7	2031.5
8	2224.0
9	2143.0
10	2062.0
11	2303.0
12	2544.0
13	2689.5
14	2835.0
15	3483.5
16	4132.0
17	3709.0
18	3286.0
19	3929.5
20	4573.0
21	5150.0
22	5727.0
23	8364.0
24	11001.0
25	10451.5
26	12434.5
27	14967.0
28	21997.5
29	29028.0
30	32108.0
31	35188.0
32	43367.0
33	51546.0
34	52864.5
35	54183.0
36	57421.0
37	60659.0
38	64216.0
39	67773.0
40	72209.0
41	76645.0
42	76876.5
43	77108.0
44	87336.0
45	97564.0
46	100496.5
47	103429.0
48	108126.0
49	112823.0
50	111825.0
51	110827.0
52	105950.0
53	101073.0
54	97617.0
55	94161.0
56	88806.0
57	83451.0
58	80566.0
59	77681.0
60	71107.0
61	64533.0
62	60460.0
63	56387.0
64	50615.0
65	44843.0
66	38257.5
67	31672.0
68	27796.0
69	23920.0
70	20527.5
71	17135.0
72	13874.5
73	10614.0
74	9495.0
75	6381.0
76	4386.0
77	3680.5
78	2975.0
79	2034.5
80	1094.0
81	908.5
82	723.0
83	600.5
84	478.0
85	315.5
86	153.0
87	112.5
88	72.0
89	51.0
90	30.0
91	27.0
92	24.0
93	19.0
94	14.0
95	13.0
96	12.0
97	6.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1592164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.54112328534607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44885386095596	14.359887477447353
2	9.036681530827249	3.351004527069558
3	3.3443016849746168	1.860213295335149
4	1.668581909788238	1.2374953160432767
5	1.0311049284193923	0.9558921798975945
6	0.6478216943211603	0.7206805140798247
7	0.49672675624586127	0.6446910418679193
8	0.3702701470228617	0.5492179555867275
9	0.28261007262001875	0.4715917378315536
>10	3.0496378660179153	13.426847406931694
>50	1.2488780183891874	16.96386564770701
>100	1.3647855484344047	42.73234425694395
>500	0.0062652741320630215	0.7160295094439905
>1k	0.0031326370660315107	1.1351923453014683
>5k	0.0	0.0
>10k+	3.4807078511461234E-4	0.8750467885129396
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13559	0.8516082514112868	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2732	0.17159036380674353	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	2536	0.15928007416321435	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2378	0.1493564733281245	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2256	0.14169394610102978	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1932	0.12134428362907339	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1652	0.10375815556688883	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.512304008883507E-4	0.0	0.0	0.19137475787670113	0.0
2	2.512304008883507E-4	0.0	0.0	0.2543707808994551	0.0
3	2.512304008883507E-4	0.0	0.0	0.3313728987717346	0.0
4	3.140380011104384E-4	0.0	0.0	0.6932702912514037	0.0
5	3.140380011104384E-4	0.0	0.0	0.7487294022475072	0.0
6	3.140380011104384E-4	0.0	0.0	1.124758504777146	0.0
7	3.140380011104384E-4	0.0	0.0	1.4072670905760964	0.0
8	3.7684560133252604E-4	0.0	0.0	1.6740109687193028	0.0
9	3.7684560133252604E-4	0.0	0.0	2.0542481804638215	0.0
10	3.7684560133252604E-4	0.0	0.0	2.625546112083931	0.0
11	4.3965320155461374E-4	0.0	0.0	3.1516225715441375	0.0
12	4.3965320155461374E-4	0.0	0.0	3.39267814119651	0.0
13	4.3965320155461374E-4	0.0	0.0	3.498948600772282	0.0
14	4.3965320155461374E-4	0.0	0.0	3.6201672692009113	0.0
15	4.3965320155461374E-4	0.0	0.0	3.7261864983757955	0.0
16	4.3965320155461374E-4	0.0	0.0	3.9324466575051313	0.0
17	5.024608017767014E-4	0.0	0.0	4.1880107828088065	0.0
18	5.024608017767014E-4	0.0	0.0	4.5123492303556665	0.0
19	5.024608017767014E-4	0.0	0.0	4.6529754472529214	0.0
20	5.024608017767014E-4	0.0	0.0	4.8224303526521135	0.0
21	5.024608017767014E-4	0.0	0.0	5.007901196107938	0.0
22	5.024608017767014E-4	0.0	0.0	5.2179298112506	0.0
23	5.024608017767014E-4	0.0	0.0	5.421112397969053	0.0
24	5.024608017767014E-4	0.0	0.0	5.573609251308282	0.0
25	5.024608017767014E-4	0.0	0.0	5.718820423021749	0.0
26	5.024608017767014E-4	0.0	0.0	5.8433050866619265	0.0
27	5.024608017767014E-4	0.0	0.0	5.990902947183833	0.0
28	5.024608017767014E-4	0.0	0.0	6.134229890890637	0.0
29	5.024608017767014E-4	0.0	0.0	6.293572772654073	0.0
30	5.024608017767014E-4	0.0	0.0	6.519994171454699	0.0
31	5.024608017767014E-4	0.0	0.0	6.665142535567944	0.00540145361909954
32	5.65268401998789E-4	0.0	0.0	6.821659075321387	0.00540145361909954
33	5.65268401998789E-4	0.0	0.0	6.976354194668389	0.00540145361909954
34	5.65268401998789E-4	0.0	0.0	7.122256249984298	0.00540145361909954
35	5.65268401998789E-4	0.0	0.0	7.291459924982602	0.00540145361909954
36	5.65268401998789E-4	0.0	0.0	7.447599619134713	0.00540145361909954
37	5.65268401998789E-4	0.0	0.0	7.603299660085268	0.00540145361909954
38	5.65268401998789E-4	0.0	0.0	7.766599420662695	0.00540145361909954
39	5.65268401998789E-4	0.0	0.0	7.94905549930786	0.00540145361909954
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAC	35	1.21232E-7	45.000004	19
CGGCACG	85	0.0	45.000004	1
GCGCCTA	35	1.21232E-7	45.000004	32
CGTAATG	70	0.0	45.000004	1
GCGCGTA	20	7.0341065E-4	45.0	17
CGGCATA	20	7.0341065E-4	45.0	39
ATAGCGC	25	3.8914863E-5	45.0	36
TACGGTT	25	3.8914863E-5	45.0	28
CGTTCGA	25	3.8914863E-5	45.0	20
CCGTCGA	25	3.8914863E-5	45.0	36
TAGTTCG	25	3.8914863E-5	45.0	1
TTGAGCG	75	0.0	45.0	1
AATGCGA	20	7.0341065E-4	45.0	27
CCGTAGA	20	7.0341065E-4	45.0	10
TACCTCG	20	7.0341065E-4	45.0	1
TATAGCG	40	6.8175723E-9	45.0	1
CGCCGTA	20	7.0341065E-4	45.0	34
CGGTACC	20	7.0341065E-4	45.0	34
ACATATC	55	1.8189894E-12	45.0	32
CGATAGC	25	3.8914863E-5	45.0	45
>>END_MODULE