FastQCFastQC Report
Sat 18 Jun 2016
SRR3553367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578916
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133870.8478601774888594No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28600.1811369319203808No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23780.1506096587785544No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT23740.1503563204122322No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA21850.1383860826035077No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18710.11849902084721416No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG16250.10291871131839819No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA302.1660198E-645.00000417
GTCCGAT302.1660198E-645.00000442
CGTAATG850.045.0000041
GTATTCG207.034089E-445.021
TAGACGC207.034089E-445.029
TTTGTCG253.8914703E-545.013
TAGCGAT253.8914703E-545.045
ATAGCGC207.034089E-445.036
TAGTTCG253.8914703E-545.01
CCGTAAC207.034089E-445.036
CTACGAT406.8175723E-945.033
CGAATTA406.8175723E-945.037
TTGCGAC253.8914703E-545.024
GCGATTA253.8914703E-545.023
CGTAACA207.034089E-445.037
ATACGCG406.8175723E-945.01
GCTCGTA406.8175723E-945.032
TTCGATA207.034089E-445.035
TAAGACG1050.045.01
CGATAGC207.034089E-445.045