##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623077
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.563026720613983	31.0	31.0	34.0	30.0	34.0
2	31.761711634356587	31.0	31.0	34.0	30.0	34.0
3	31.929609021035922	33.0	31.0	34.0	30.0	34.0
4	35.66144954796919	37.0	35.0	37.0	33.0	37.0
5	35.4055293326507	37.0	35.0	37.0	33.0	37.0
6	34.08535221168491	37.0	35.0	37.0	32.0	37.0
7	34.94141173562818	37.0	35.0	37.0	32.0	37.0
8	35.63221881083718	37.0	35.0	37.0	33.0	37.0
9	37.286834532489564	39.0	37.0	39.0	34.0	39.0
10	36.65322424034269	38.0	35.0	39.0	32.0	39.0
11	36.682259175029735	39.0	37.0	39.0	32.0	39.0
12	36.75616336343662	39.0	37.0	39.0	32.0	39.0
13	36.75243990710619	39.0	37.0	39.0	32.0	39.0
14	37.91512284998483	40.0	37.0	41.0	33.0	41.0
15	37.91225322070948	40.0	37.0	41.0	33.0	41.0
16	37.7856557054746	39.0	37.0	41.0	33.0	41.0
17	37.76338076995299	39.0	37.0	41.0	33.0	41.0
18	37.61661560288696	39.0	37.0	41.0	33.0	41.0
19	37.57135955909141	39.0	36.0	41.0	32.0	41.0
20	37.46208895529766	39.0	36.0	41.0	32.0	41.0
21	37.420742540649066	39.0	36.0	41.0	32.0	41.0
22	37.40394686371027	39.0	36.0	41.0	32.0	41.0
23	37.259128486527345	39.0	36.0	40.0	32.0	41.0
24	37.07140850970265	39.0	35.0	40.0	31.0	41.0
25	37.15510924010997	39.0	35.0	40.0	32.0	41.0
26	37.05533665983498	39.0	35.0	40.0	31.0	41.0
27	37.04009295801322	39.0	35.0	40.0	31.0	41.0
28	36.97516197837506	39.0	35.0	40.0	31.0	41.0
29	36.74971311731937	39.0	35.0	40.0	31.0	41.0
30	36.82754137931588	39.0	35.0	40.0	31.0	41.0
31	36.724311762430645	39.0	35.0	40.0	31.0	41.0
32	36.565450177104914	39.0	35.0	40.0	30.0	41.0
33	36.54671092015914	39.0	35.0	40.0	30.0	41.0
34	36.4550159932079	39.0	35.0	40.0	30.0	41.0
35	36.28876366805387	38.0	35.0	40.0	30.0	41.0
36	36.268764534720425	38.0	35.0	40.0	30.0	41.0
37	36.193992074815796	38.0	35.0	40.0	30.0	41.0
38	36.124551219191204	38.0	35.0	40.0	30.0	41.0
39	36.05671851151623	38.0	35.0	40.0	30.0	41.0
40	35.965216177133804	38.0	35.0	40.0	29.0	41.0
41	35.869038658143374	38.0	35.0	40.0	29.0	41.0
42	35.778707928554574	38.0	35.0	40.0	28.0	41.0
43	35.685520409195014	38.0	35.0	40.0	28.0	41.0
44	35.57417301553419	38.0	34.0	40.0	28.0	41.0
45	35.408886863100385	38.0	34.0	40.0	27.0	41.0
46	35.22854478659941	38.0	34.0	40.0	26.0	41.0
47	35.17105108999369	38.0	34.0	40.0	26.0	41.0
48	35.02856147795537	38.0	34.0	40.0	26.0	41.0
49	34.84908606801407	37.0	33.0	40.0	26.0	41.0
50	34.75777311632431	37.0	33.0	40.0	26.0	41.0
51	33.63563893387174	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	6.0
11	3.0
12	3.0
13	8.0
14	13.0
15	39.0
16	64.0
17	128.0
18	305.0
19	528.0
20	964.0
21	1408.0
22	2032.0
23	2797.0
24	3393.0
25	4282.0
26	5109.0
27	6397.0
28	7997.0
29	10209.0
30	13469.0
31	17205.0
32	22378.0
33	29574.0
34	41827.0
35	60863.0
36	65592.0
37	85834.0
38	120060.0
39	120562.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.729985218520344	31.94003309382307	22.44778735212502	25.882194335531562
2	20.996602346098474	34.52526734255959	20.842046809623852	23.636083501718087
3	20.007960492844383	34.98636605106592	20.996762839905823	24.008910616183872
4	19.44976303089345	35.67697090407766	20.0387753038549	24.83449076117398
5	16.458479449570437	40.074982706792255	17.2949731734601	26.1715646701772
6	19.264071695793618	42.15562442523155	18.17415825010392	20.40614562887091
7	82.4976688274483	10.261171572694867	3.9719007442097847	3.269258855647055
8	83.97694024976047	9.132258131819984	3.243900834086317	3.6469007843332366
9	76.45106463567103	14.567541411414641	5.7673449669944485	3.214048985919878
10	28.404675505595616	49.45183340100822	10.95562827708293	11.187862816313231
11	17.97145457142536	31.333045514438822	33.66261312807246	17.032886786063358
12	22.47298487987841	28.18544096476037	32.20211948121982	17.1394546741414
13	22.010281233298613	30.517255491696854	27.899761987683707	19.57270128732083
14	17.220825034466046	33.04567493263272	29.767909905196312	19.96559012770492
15	16.0992943087291	36.23324244034044	28.23679898311124	19.430664267819225
16	18.193096519370798	35.59399560567956	27.264046016784445	18.948861858165202
17	17.18984972964818	35.114119121713685	28.376428595502645	19.319602553135486
18	17.496071914065194	34.39799575333386	28.087058260857006	20.01887407174394
19	18.260503918456305	36.19231651946709	28.32876193472075	17.218417627355848
20	20.240676513496727	35.43960056301228	27.132601588567706	17.18712133492329
21	19.99512098825667	35.68579806348172	26.858799153234674	17.460281795026937
22	18.60989893704951	33.54994647531525	27.559033634687207	20.281120952948033
23	17.949145932204207	35.0529709811147	26.031935057785795	20.965948028895305
24	15.802862246560217	36.54764980893212	27.933626181033805	19.715861763473857
25	17.79282496384877	36.966538646106336	25.632144983685805	19.608491406359086
26	19.153972943953956	36.406254764659906	25.077317891689148	19.362454399696986
27	17.42914599640173	36.79176088990606	25.80242891328038	19.97666420041183
28	19.070676657941156	35.69173633435354	26.894428778465585	18.343158229239727
29	18.672010040492587	37.629859551869195	24.4364661189548	19.261664288683424
30	20.04696048802957	35.17446479327595	25.973354818104344	18.805219900590135
31	20.73917027911478	36.26100786901137	23.035997156049735	19.963824695824112
32	19.775725953614078	36.668020164441955	24.37387353408969	19.182380347854277
33	20.497145617636345	35.75192151210845	24.685713001763826	19.065219868491372
34	18.887071742336822	35.77808200270592	24.770614225850096	20.564232029107156
35	20.67465176856151	36.979859632116096	24.34305872307917	18.002429876243227
36	20.160269116016156	36.77137817637306	25.62925609515357	17.43909661245721
37	20.289306137122697	37.04678554977956	24.019182219854045	18.64472609324369
38	19.83719508182777	35.19115614923998	26.715638677081643	18.256010091850605
39	18.708763122374922	34.80147718500282	26.64052757524351	19.84923211737875
40	20.041503698579792	36.04642764858918	24.075194558617955	19.836874094213076
41	17.809355826005454	34.6510944875192	27.145120105540727	20.39442958093462
42	20.333602427950318	35.393217852689155	24.147737759538547	20.12544195982198
43	19.00134333316749	34.93966235312811	26.45210784541878	19.606886468285623
44	20.833380144027142	33.049045302587	25.2150215783924	20.902552974993462
45	21.373281311940577	33.526674873250016	25.973515311911687	19.126528502897717
46	18.062936041612833	34.90050186413558	28.02767555213882	19.008886542112773
47	19.607688937322354	34.393501926728156	25.332984526792035	20.665824609157454
48	18.962664325596997	33.45461315375146	28.282379224397623	19.300343296253914
49	20.01165185041335	33.8545637216588	25.934194329111808	20.199590098816035
50	19.33083711964974	32.86768730028552	25.61160177634546	22.189873803719284
51	18.19534343267365	31.93409482295126	28.21994713333986	21.65061461103523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19451.0
1	15013.0
2	10575.0
3	6426.0
4	2277.0
5	1822.5
6	1368.0
7	1434.0
8	1500.0
9	1989.5
10	2479.0
11	3082.5
12	3686.0
13	4169.0
14	4652.0
15	5201.5
16	5751.0
17	5386.5
18	5022.0
19	5237.5
20	5453.0
21	4828.5
22	4204.0
23	5066.0
24	5928.0
25	6631.5
26	8606.5
27	9878.0
28	12536.0
29	15194.0
30	14828.0
31	14462.0
32	17236.5
33	20011.0
34	22668.0
35	25325.0
36	26950.5
37	28576.0
38	28261.5
39	27947.0
40	29344.5
41	30742.0
42	34228.5
43	37715.0
44	40552.0
45	43389.0
46	46264.0
47	49139.0
48	51448.5
49	53758.0
50	51263.5
51	48769.0
52	43432.0
53	38095.0
54	32933.0
55	27771.0
56	23485.0
57	19199.0
58	16997.5
59	14796.0
60	13659.0
61	12522.0
62	10860.5
63	9199.0
64	7734.5
65	6270.0
66	5313.0
67	4356.0
68	3499.0
69	2642.0
70	2050.0
71	1458.0
72	1248.5
73	1039.0
74	796.5
75	417.5
76	281.0
77	226.5
78	172.0
79	120.0
80	68.0
81	42.0
82	16.0
83	14.0
84	12.0
85	11.0
86	10.0
87	9.5
88	9.0
89	8.5
90	8.0
91	8.0
92	8.0
93	6.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	623077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.433566000855723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50728410393647	20.714340620107055
2	9.707039278050827	5.325974054146125
3	3.661056669719155	3.0130751934484055
4	1.9357366152206295	2.124166327757128
5	1.2999609252534163	1.7831281920736537
6	0.9339094852643741	1.5372280501695244
7	0.7468142600243628	1.434144480493101
8	0.6004994126020392	1.317907221527451
9	0.48410877012105785	1.1952746907038219
>10	4.003077756687682	23.32178077030349
>50	0.7001722359813685	13.662558928668227
>100	0.4114475290186873	18.171692932672823
>500	0.0035571832480001783	0.6178834252349392
>1k	0.004742910997333572	2.9692234301366285
>5k	0.0	0.0
>10k+	5.928638746666965E-4	2.811621682557619
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17287	2.7744564475979696	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4202	0.6743949784697558	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3397	0.5451974635558687	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	3143	0.504432036489872	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1710	0.2744444105624184	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	1515	0.24314811812986195	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGT	1473	0.23640737822131133	No Hit
CTTTTTGGGAGAGCATAATCACATACATAACTACACACTCATTTGTGTAGC	1455	0.23351848968907538	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	1361	0.21843207179850968	No Hit
CAATATGGGAAAAACAAACAAACAAACAAACAAACAAACAAACCCATAAAC	766	0.12293825642737576	No Hit
TTGCTGGGGCCGATGGCGTGCGGGATGCCAGTATTACAAAATTTTAAAAAA	694	0.11138270229843182	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	662	0.10624690046334563	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.814814220393306E-4	0.0	0.0	0.6132468378707607	0.0
2	4.814814220393306E-4	0.0	0.0	0.8145060722832009	0.0
3	4.814814220393306E-4	0.0	0.0	1.030530736971514	0.0
4	4.814814220393306E-4	0.0	0.0	2.159604671653744	0.0
5	4.814814220393306E-4	0.0	0.0	2.3199379851928414	0.0
6	4.814814220393306E-4	0.0	0.0	3.2108391097729494	0.0
7	4.814814220393306E-4	0.0	0.0	3.9463822288417	0.0
8	4.814814220393306E-4	0.0	0.0	4.55786363483165	0.0
9	4.814814220393306E-4	0.0	0.0	5.75145608006715	0.0
10	4.814814220393306E-4	0.0	0.0	7.194776889533717	0.0
11	4.814814220393306E-4	0.0	0.0	8.67212238615773	0.0
12	4.814814220393306E-4	0.0	0.0	9.311690208433308	0.0
13	4.814814220393306E-4	0.0	0.0	9.570406225875775	0.0
14	4.814814220393306E-4	0.0	0.0	9.874060509375246	0.0
15	4.814814220393306E-4	0.0	0.0	10.13245553920302	0.0
16	4.814814220393306E-4	0.0	0.0	10.625332021563947	0.0
17	4.814814220393306E-4	0.0	0.0	11.3111220603553	0.0
18	4.814814220393306E-4	0.0	0.0	12.175220719108554	0.0
19	4.814814220393306E-4	0.0	0.0	12.551578697335962	0.0
20	4.814814220393306E-4	0.0	0.0	12.950726796206569	0.0
21	4.814814220393306E-4	0.0	0.0	13.35180082076533	0.0
22	4.814814220393306E-4	0.0	0.0	13.83697360037363	0.0
23	4.814814220393306E-4	0.0	0.0	14.296627864613844	0.0
24	4.814814220393306E-4	0.0	0.0	14.617294491692038	0.0
25	4.814814220393306E-4	0.0	0.0	14.884516680923866	0.0
26	4.814814220393306E-4	0.0	0.0	15.155269733917317	0.0
27	4.814814220393306E-4	0.0	0.0	15.43757834103971	0.0
28	4.814814220393306E-4	0.0	0.0	15.734331390823286	0.0
29	4.814814220393306E-4	0.0	0.0	16.06703505345246	0.0
30	4.814814220393306E-4	0.0	0.0	16.468590559433263	0.0
31	4.814814220393306E-4	0.0	0.0	16.770960892473965	0.0
32	4.814814220393306E-4	0.0	0.0	17.04460283399965	0.0
33	4.814814220393306E-4	0.0	0.0	17.329479342039587	0.0
34	4.814814220393306E-4	0.0	0.0	17.62591140420847	0.0
35	4.814814220393306E-4	0.0	0.0	17.958294079222952	0.0
36	4.814814220393306E-4	0.0	0.0	18.249750833364093	0.0
37	4.814814220393306E-4	0.0	0.0	18.538158205165654	0.0
38	4.814814220393306E-4	0.0	0.0	18.85063964806918	0.0
39	4.814814220393306E-4	0.0	0.0	19.154133437761303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCG	20	7.0307957E-4	45.000004	1
TCGCCCG	20	7.0307957E-4	45.000004	16
CGGCGTG	20	7.0307957E-4	45.000004	1
CCTTACG	20	7.0307957E-4	45.000004	1
CAAACGC	20	7.0307957E-4	45.000004	35
TCCGCTC	20	7.0307957E-4	45.000004	11
TCCGCAC	20	7.0307957E-4	45.000004	27
CGAAACG	20	7.0307957E-4	45.000004	1
CGGCCTA	40	6.8066583E-9	45.000004	42
CGTTGTC	35	1.2107375E-7	45.000004	45
TAGCGTT	20	7.0307957E-4	45.000004	31
CGGCATG	40	6.8066583E-9	45.000004	1
CGGCACT	20	7.0307957E-4	45.000004	1
TTGTCGA	20	7.0307957E-4	45.000004	36
CCGTGCG	30	2.1638953E-6	45.000004	1
CGATTAG	35	1.2107375E-7	45.000004	1
CGACAGA	20	7.0307957E-4	45.000004	38
CCGCTGA	35	1.2107375E-7	45.000004	36
TCTAGCG	20	7.0307957E-4	45.000004	1
GCGCAGC	40	6.8066583E-9	45.000004	35
>>END_MODULE