FastQCFastQC Report
Sat 18 Jun 2016
SRR3553365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616150
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171032.7757851172604076No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39940.648218777894993No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32620.5294165381806378No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT29540.47942871054126435No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16890.27412156130812304No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC13810.2241337336687495No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGT13690.22218615596851415No Hit
CTTTTTGGGAGAGCATAATCACATACATAACTACACACTCATTTGTGTAGC13510.2192647894181612No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT12190.1978414347155725No Hit
CAATATGGGAAAAACAAACAAACAAACAAACAAACAAACAAACCCATAAAC7330.11896453785604155No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6800.11036273634666882No Hit
TTGCTGGGGCCGATGGCGTGCGGGATGCCAGTATTACAAAATTTTAAAAAA6640.10776596607968839No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT253.888691E-545.00000436
TGATCGG253.888691E-545.0000042
CGGCGTG253.888691E-545.0000041
AGCCGTC502.1827873E-1145.00000438
CGTATAT502.1827873E-1145.00000412
GTCGAGA253.888691E-545.00000438
CACGGTT253.888691E-545.00000414
CAACGCT253.888691E-545.00000422
TCCGATA253.888691E-545.00000424
TTGTCGA253.888691E-545.00000436
CCGTGCG253.888691E-545.0000041
CATTCGC253.888691E-545.00000417
ATCGTTA253.888691E-545.00000438
AAGCCGT502.1827873E-1145.00000437
TCGGAAC253.888691E-545.00000424
CGCTCAG253.888691E-545.0000041
CACTACG253.888691E-545.0000041
AACGCTA253.888691E-545.00000423
GATATCG253.888691E-545.00000420
ACGCTAG253.888691E-545.0000041