##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	616150
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.530177716465147	31.0	31.0	34.0	30.0	34.0
2	31.743901647326137	31.0	31.0	34.0	30.0	34.0
3	31.921773918688633	33.0	31.0	34.0	30.0	34.0
4	35.6561308123022	37.0	35.0	37.0	33.0	37.0
5	35.42134382861316	37.0	35.0	37.0	33.0	37.0
6	32.73104601152317	37.0	35.0	37.0	26.0	37.0
7	34.269538261786906	37.0	35.0	37.0	28.0	37.0
8	35.35751359246937	37.0	35.0	37.0	32.0	37.0
9	37.177271768238256	39.0	37.0	39.0	34.0	39.0
10	36.61090805810274	38.0	35.0	39.0	32.0	39.0
11	36.69085287673456	39.0	37.0	39.0	32.0	39.0
12	36.769536638805484	39.0	37.0	39.0	32.0	39.0
13	36.78685547350483	39.0	37.0	39.0	33.0	39.0
14	37.915332305445105	40.0	37.0	41.0	33.0	41.0
15	37.95502231599448	40.0	37.0	41.0	33.0	41.0
16	37.85589872595959	40.0	37.0	41.0	33.0	41.0
17	37.849315913332795	39.0	37.0	41.0	33.0	41.0
18	37.70776434309827	39.0	37.0	41.0	33.0	41.0
19	37.66573237036436	39.0	36.0	41.0	33.0	41.0
20	37.539511482593525	39.0	36.0	41.0	32.0	41.0
21	37.4715393978739	39.0	36.0	41.0	32.0	41.0
22	37.41441532094458	39.0	36.0	41.0	32.0	41.0
23	37.28829343504017	39.0	36.0	40.0	32.0	41.0
24	37.17255538424085	39.0	35.0	40.0	32.0	41.0
25	37.2245524628743	39.0	35.0	40.0	32.0	41.0
26	37.13083502393898	39.0	35.0	40.0	32.0	41.0
27	37.06570964862453	39.0	35.0	40.0	32.0	41.0
28	37.038276393735295	39.0	35.0	40.0	31.0	41.0
29	36.77869999188509	39.0	35.0	40.0	31.0	41.0
30	36.85537450296194	39.0	35.0	40.0	31.0	41.0
31	36.4273245151343	39.0	35.0	40.0	30.0	41.0
32	36.479149557737564	39.0	35.0	40.0	30.0	41.0
33	36.503106386431874	39.0	35.0	40.0	30.0	41.0
34	36.458932078227704	39.0	35.0	40.0	30.0	41.0
35	36.39500284021748	38.0	35.0	40.0	30.0	41.0
36	36.33305526251724	38.0	35.0	40.0	30.0	41.0
37	36.23811734155644	38.0	35.0	40.0	30.0	41.0
38	36.164849468473584	38.0	35.0	40.0	30.0	41.0
39	36.078042684411265	38.0	35.0	40.0	30.0	41.0
40	36.016736184370686	38.0	35.0	40.0	29.0	41.0
41	35.977979388136006	38.0	35.0	40.0	29.0	41.0
42	35.886603911385215	38.0	35.0	40.0	29.0	41.0
43	35.81960723849712	38.0	35.0	40.0	29.0	41.0
44	35.63617787876329	38.0	35.0	40.0	28.0	41.0
45	35.48166355595228	38.0	34.0	40.0	27.0	41.0
46	35.17646352349266	38.0	34.0	40.0	26.0	41.0
47	35.185294165381805	38.0	34.0	40.0	26.0	41.0
48	35.17056885498661	38.0	34.0	40.0	26.0	41.0
49	35.03085125375314	37.0	34.0	40.0	26.0	41.0
50	34.909612918932076	37.0	34.0	40.0	26.0	41.0
51	33.863680921853444	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	8.0
11	7.0
12	2.0
13	12.0
14	15.0
15	26.0
16	65.0
17	141.0
18	283.0
19	516.0
20	924.0
21	1465.0
22	2002.0
23	2688.0
24	3441.0
25	4099.0
26	4889.0
27	6260.0
28	7979.0
29	9988.0
30	13052.0
31	16831.0
32	22224.0
33	29418.0
34	41987.0
35	60502.0
36	66115.0
37	87843.0
38	116745.0
39	116593.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.660147691308936	31.927452730666232	22.54580865049095	25.866590927533878
2	21.163190781465552	34.388866347480324	20.759717601233465	23.688225269820663
3	19.93069869349996	35.081554816197354	20.93353891097947	24.05420757932322
4	19.4937920960805	35.76012334658768	19.940598880142822	24.805485677188997
5	16.500365170818796	39.9715978252049	17.35697476263897	26.171062241337335
6	18.50523411506938	44.43982796396981	17.500770916173007	19.554167004787796
7	82.53850523411506	10.287105412643026	3.9125213016310965	3.261868051610809
8	83.94563012253509	9.14192972490465	3.206362087154102	3.706078065406151
9	76.60212610565608	14.544997159782522	5.658849306175444	3.1940274283859447
10	28.445183802645456	49.633855392355755	10.904325245475938	11.016635559522843
11	17.99139819849063	31.285401282155316	33.62411750385458	17.099083015499474
12	22.488842002759068	28.26503286537369	32.110525034488354	17.135600097378887
13	22.141037085125376	30.452649517163028	27.88363223241094	19.522681165300657
14	17.225188671589713	33.055262517244174	29.759636452162624	19.95991235900349
15	16.096729692445024	36.253834293597336	28.242148827395923	19.407287186561714
16	18.18712975736428	35.59117098109227	27.41231842895399	18.80938083258947
17	17.129108171711437	35.30536395358273	28.247991560496633	19.317536314209203
18	17.535340420352185	34.4706646108902	28.080337580134707	19.913657388622898
19	18.202872677107848	36.16213584354459	28.432686845735617	17.202304633611945
20	20.259514728556358	35.38910979469285	27.104763450458492	17.2466120262923
21	20.12253509697314	35.67167085936866	26.82983039844194	17.375963645216263
22	18.787145987178448	33.47821147447862	27.60107116773513	20.133571370607804
23	17.928751115799724	35.16773512943277	26.078227704292782	20.825286050474723
24	15.844193783981172	36.4399902621115	28.10354621439585	19.612269739511483
25	17.83348210662988	36.99326462712002	25.571370607806543	19.60188265844356
26	19.2182098514972	36.366306905785926	24.999431956504097	19.416051286212774
27	17.537612594335794	36.772214558143304	25.894506207903916	19.795666639616975
28	18.877546052097706	35.81968676458654	26.929806053720682	18.372961129595065
29	18.70599691633531	37.65300657307474	24.461089020530714	19.17990749005924
30	20.144769942384162	35.229083826990184	25.917877140306743	18.708269090318915
31	20.779031080094132	36.27395926316644	23.01030593199708	19.93670372474235
32	19.691795828937757	36.783413129919666	24.357867402418243	19.166923638724338
33	20.481213990099814	35.72847520895886	24.726771078471153	19.06353972247018
34	18.852227541994644	35.78885011766616	24.81814493224053	20.540777408098677
35	20.553598961291893	37.02718493873245	24.4115880873164	18.007628012659254
36	20.086991803943846	36.91211555627688	25.572506694798346	17.428385944980928
37	20.27947739998377	37.036598230950254	24.011685466201413	18.672238902864564
38	19.827152479104114	35.1851010305932	26.78487381319484	18.202872677107848
39	18.764099651058995	35.04860829343504	26.4115880873164	19.775703968189564
40	20.021910249127647	36.18469528523898	24.18274770753875	19.610646758094617
41	17.745516513835916	34.716708593686604	27.120830966485432	20.416943925992047
42	20.20611863994157	35.542643836728075	24.115231680597258	20.136005842733102
43	18.859693256512212	35.14550028402175	26.42424734236793	19.57055911709811
44	20.782601639211233	32.999269658362415	25.22437718088128	20.99375152154508
45	21.411182341962185	33.623630609429526	25.824880305120505	19.140306743487788
46	18.206930130650004	34.91357623955206	27.893857015337176	18.985636614460766
47	19.57575265763207	34.45914144283048	25.363466688306417	20.60163921123103
48	18.87462468554735	33.68692688468717	28.169439259920477	19.269009169845006
49	20.021423354702588	34.04235981498012	25.808325894668506	20.127890935648786
50	19.2595958776272	33.07457599610484	25.679298872027918	21.98652925424004
51	18.124969569098432	32.16619329708675	28.09900186642863	21.609835267386188
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19298.0
1	14800.5
2	10303.0
3	6359.5
4	2416.0
5	1892.5
6	1369.0
7	1390.5
8	1412.0
9	1948.5
10	2485.0
11	3087.0
12	3689.0
13	4160.0
14	4631.0
15	5222.5
16	5814.0
17	5400.0
18	4986.0
19	5192.0
20	5398.0
21	4924.0
22	4450.0
23	5260.5
24	6071.0
25	6663.0
26	8591.0
27	9927.0
28	12390.5
29	14854.0
30	14665.0
31	14476.0
32	17122.0
33	19768.0
34	22573.5
35	25379.0
36	26726.0
37	28073.0
38	27916.5
39	27760.0
40	29158.5
41	30557.0
42	33945.5
43	37334.0
44	40242.5
45	43151.0
46	45753.5
47	48356.0
48	50700.0
49	53044.0
50	50421.0
51	47798.0
52	42421.5
53	37045.0
54	32261.5
55	27478.0
56	23229.5
57	18981.0
58	16800.0
59	14619.0
60	13512.5
61	12406.0
62	10744.0
63	9082.0
64	7616.0
65	6150.0
66	5139.5
67	4129.0
68	3394.5
69	2660.0
70	2042.0
71	1424.0
72	1192.0
73	960.0
74	752.5
75	410.5
76	276.0
77	238.0
78	200.0
79	138.0
80	76.0
81	48.0
82	20.0
83	16.0
84	12.0
85	9.0
86	6.0
87	6.5
88	7.0
89	6.5
90	6.0
91	5.5
92	5.0
93	5.0
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	616150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.944354373696633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07057343871166	20.977987072079767
2	9.815582963979393	5.485802574597194
3	3.6685725830124	3.0754767691597835
4	2.0522201742669797	2.2939187121026374
5	1.3784034256606603	1.9259296898289444
6	1.0021163805543134	1.6802097157137579
7	0.7376954993477329	1.44300971175579
8	0.5947252524286524	1.3295370567081957
9	0.4921829046758928	1.2378360154444648
>10	4.109981579611162	23.725167855787785
>50	0.6779016079590378	13.31529380782333
>100	0.3918200801634993	17.348753927556384
>500	0.002937182010221134	0.5269385448025358
>1k	0.004699491216353814	2.82659028129435
>5k	0.0	0.0
>10k+	5.874364020442268E-4	2.8075482653450994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17103	2.7757851172604076	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3994	0.648218777894993	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3262	0.5294165381806378	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	2954	0.47942871054126435	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1689	0.27412156130812304	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	1381	0.2241337336687495	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGT	1369	0.22218615596851415	No Hit
CTTTTTGGGAGAGCATAATCACATACATAACTACACACTCATTTGTGTAGC	1351	0.2192647894181612	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	1219	0.1978414347155725	No Hit
CAATATGGGAAAAACAAACAAACAAACAAACAAACAAACAAACCCATAAAC	733	0.11896453785604155	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	680	0.11036273634666882	No Hit
TTGCTGGGGCCGATGGCGTGCGGGATGCCAGTATTACAAAATTTTAAAAAA	664	0.10776596607968839	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6229814168627768E-4	0.0	0.0	0.5766452974113446	0.0
2	1.6229814168627768E-4	0.0	0.0	0.7657226324758581	0.0
3	1.6229814168627768E-4	0.0	0.0	0.9882333847277449	0.0
4	1.6229814168627768E-4	0.0	0.0	2.0383023614379616	0.0
5	1.6229814168627768E-4	0.0	0.0	2.188915036922827	0.0
6	1.6229814168627768E-4	0.0	0.0	3.029781708999432	0.0
7	1.6229814168627768E-4	0.0	0.0	3.7489247748113286	0.0
8	1.6229814168627768E-4	0.0	0.0	4.359165787551732	0.0
9	1.6229814168627768E-4	0.0	0.0	5.506938245557088	0.0
10	1.6229814168627768E-4	0.0	0.0	6.884200275906841	0.0
11	1.6229814168627768E-4	0.0	0.0	8.340988395682869	0.0
12	1.6229814168627768E-4	0.0	0.0	8.983526738618842	0.0
13	1.6229814168627768E-4	0.0	0.0	9.234926560090887	0.0
14	1.6229814168627768E-4	0.0	0.0	9.532256755660148	0.0
15	1.6229814168627768E-4	0.0	0.0	9.785766452974114	0.0
16	1.6229814168627768E-4	0.0	0.0	10.247504666071574	0.0
17	1.6229814168627768E-4	0.0	0.0	10.911466363710135	0.0
18	1.6229814168627768E-4	0.0	0.0	11.727339121967054	0.0
19	1.6229814168627768E-4	0.0	0.0	12.1028970218291	0.0
20	1.6229814168627768E-4	0.0	0.0	12.505234115069383	0.0
21	1.6229814168627768E-4	0.0	0.0	12.901403878925587	0.0
22	1.6229814168627768E-4	0.0	0.0	13.3743406637994	0.0
23	1.6229814168627768E-4	0.0	0.0	13.837701858313721	0.0
24	1.6229814168627768E-4	0.0	0.0	14.163434228678081	0.0
25	1.6229814168627768E-4	0.0	0.0	14.421001379534204	0.0
26	1.6229814168627768E-4	0.0	0.0	14.671752008439503	0.0
27	1.6229814168627768E-4	0.0	0.0	14.94960642700641	0.0
28	1.6229814168627768E-4	0.0	0.0	15.244502150450378	0.0
29	1.6229814168627768E-4	0.0	0.0	15.557088371338148	0.0
30	1.6229814168627768E-4	0.0	0.0	15.946603911385214	0.0
31	1.6229814168627768E-4	0.0	0.0	16.24068814412075	0.0
32	1.6229814168627768E-4	0.0	0.0	16.522113121804754	0.0
33	1.6229814168627768E-4	0.0	0.0	16.801590521788526	0.0
34	1.6229814168627768E-4	0.0	0.0	17.077497362655198	0.0
35	1.6229814168627768E-4	0.0	0.0	17.405501907003163	0.0
36	1.6229814168627768E-4	0.0	0.0	17.687089182828856	0.0
37	1.6229814168627768E-4	0.0	0.0	17.984581676539804	0.0
38	1.6229814168627768E-4	0.0	0.0	18.274608455733183	0.0
39	1.6229814168627768E-4	0.0	0.0	18.58378641564554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	25	3.888691E-5	45.000004	36
TGATCGG	25	3.888691E-5	45.000004	2
CGGCGTG	25	3.888691E-5	45.000004	1
AGCCGTC	50	2.1827873E-11	45.000004	38
CGTATAT	50	2.1827873E-11	45.000004	12
GTCGAGA	25	3.888691E-5	45.000004	38
CACGGTT	25	3.888691E-5	45.000004	14
CAACGCT	25	3.888691E-5	45.000004	22
TCCGATA	25	3.888691E-5	45.000004	24
TTGTCGA	25	3.888691E-5	45.000004	36
CCGTGCG	25	3.888691E-5	45.000004	1
CATTCGC	25	3.888691E-5	45.000004	17
ATCGTTA	25	3.888691E-5	45.000004	38
AAGCCGT	50	2.1827873E-11	45.000004	37
TCGGAAC	25	3.888691E-5	45.000004	24
CGCTCAG	25	3.888691E-5	45.000004	1
CACTACG	25	3.888691E-5	45.000004	1
AACGCTA	25	3.888691E-5	45.000004	23
GATATCG	25	3.888691E-5	45.000004	20
ACGCTAG	25	3.888691E-5	45.000004	1
>>END_MODULE