##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631744
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.421301983081754	31.0	31.0	34.0	30.0	34.0
2	31.634141361057644	31.0	31.0	34.0	30.0	34.0
3	31.810503938304123	31.0	31.0	34.0	30.0	34.0
4	35.57037660824638	37.0	35.0	37.0	33.0	37.0
5	35.292096165535405	37.0	35.0	37.0	33.0	37.0
6	33.90727098318306	37.0	35.0	37.0	31.0	37.0
7	34.8156753368453	37.0	35.0	37.0	32.0	37.0
8	35.53756743237767	37.0	35.0	37.0	32.0	37.0
9	37.15972925742073	39.0	37.0	39.0	34.0	39.0
10	36.44440944433188	38.0	35.0	39.0	32.0	39.0
11	36.551451220747644	38.0	35.0	39.0	32.0	39.0
12	36.65697656012562	39.0	35.0	39.0	32.0	39.0
13	36.641128368453046	39.0	35.0	39.0	32.0	39.0
14	37.76941134383548	39.0	37.0	41.0	33.0	41.0
15	37.77295233512309	39.0	37.0	41.0	33.0	41.0
16	37.626309074561846	39.0	36.0	41.0	32.0	41.0
17	37.63004951372708	39.0	37.0	40.0	32.0	41.0
18	37.48522344493972	39.0	36.0	40.0	32.0	41.0
19	37.41184562101104	39.0	36.0	40.0	32.0	41.0
20	37.33500595177794	39.0	36.0	40.0	32.0	41.0
21	37.25280493364401	39.0	36.0	40.0	32.0	41.0
22	37.22244928325398	39.0	35.0	40.0	32.0	41.0
23	37.072804807010435	39.0	35.0	40.0	31.0	41.0
24	36.88413502937899	39.0	35.0	40.0	31.0	41.0
25	36.91833559163205	39.0	35.0	40.0	31.0	41.0
26	36.89317191773883	39.0	35.0	40.0	31.0	41.0
27	36.85253836997265	39.0	35.0	40.0	31.0	41.0
28	36.74579101661433	39.0	35.0	40.0	31.0	41.0
29	36.57486418549286	39.0	35.0	40.0	30.0	41.0
30	36.60311296981055	39.0	35.0	40.0	30.0	41.0
31	36.52295075220342	39.0	35.0	40.0	30.0	41.0
32	36.41026586718671	38.0	35.0	40.0	30.0	41.0
33	36.362878001215684	38.0	35.0	40.0	30.0	41.0
34	36.34201037128964	38.0	35.0	40.0	30.0	41.0
35	36.23505407253571	38.0	35.0	40.0	30.0	41.0
36	36.17251133370479	38.0	35.0	40.0	30.0	41.0
37	36.13480460439672	38.0	35.0	40.0	30.0	41.0
38	36.08187177084389	38.0	35.0	40.0	30.0	41.0
39	35.99530031151859	38.0	35.0	40.0	30.0	41.0
40	35.80371004710769	38.0	35.0	40.0	29.0	41.0
41	35.74260459933137	38.0	35.0	40.0	29.0	41.0
42	35.68300292523554	38.0	35.0	40.0	28.0	41.0
43	35.63046898743795	38.0	35.0	40.0	28.0	41.0
44	35.516315152973355	38.0	34.0	40.0	27.0	41.0
45	35.37400117769223	38.0	34.0	40.0	27.0	41.0
46	35.19567103130382	38.0	34.0	40.0	26.0	41.0
47	35.12337750734475	38.0	34.0	40.0	26.0	41.0
48	34.98753767348799	38.0	33.0	40.0	26.0	41.0
49	34.813150579981766	37.0	33.0	40.0	26.0	41.0
50	34.73168878533077	37.0	33.0	40.0	25.0	41.0
51	33.581892348799514	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	1.0
11	5.0
12	4.0
13	8.0
14	9.0
15	33.0
16	59.0
17	116.0
18	282.0
19	578.0
20	1047.0
21	1714.0
22	2431.0
23	3103.0
24	3705.0
25	4284.0
26	5252.0
27	6763.0
28	8465.0
29	11034.0
30	14245.0
31	18616.0
32	24103.0
33	31391.0
34	43447.0
35	62341.0
36	68422.0
37	88040.0
38	117867.0
39	114350.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.51240375848445	35.726021932934856	20.422987792523553	25.338586516057138
2	19.74122429338466	34.41932175058252	21.834793840543004	24.004660115489816
3	19.264290598723534	35.209673538648566	21.400124100901632	24.125911761726268
4	18.539155100800325	34.859690001013064	19.616331931921792	26.984822966264815
5	16.712465808935264	37.524376962820384	18.705203373518387	27.057953854725962
6	18.278131648262587	42.35560606828082	18.84228421639145	20.52397806706514
7	81.68957679059872	9.736222267247491	4.507838618174451	4.066362323979334
8	83.39390639246277	8.662369567419715	3.564576790598724	4.379147249518792
9	75.98790016209097	13.298741262283457	7.0338618174450405	3.679496758180529
10	26.49554249822713	50.12647528112654	10.627880913787864	12.750101306858475
11	16.647882686657887	31.877785938608046	34.285406747036774	17.188924627697293
12	21.922645881876203	27.491515550602774	33.222317900921894	17.36352066659913
13	22.47144412926755	29.40368250430554	28.924691014081656	19.200182352345255
14	16.217961706007497	32.62587377165434	29.640803869921996	21.515360652416167
15	15.426660166143247	36.29666447168473	28.97328664775605	19.303388714415966
16	16.774991135649884	35.60524516259751	27.56258864350116	20.057175058251445
17	16.56113868908925	33.82604346064228	29.099445344949853	20.51337250531861
18	18.285571370681794	32.90256812886233	27.27655506027758	21.535305440178302
19	17.619953652112248	36.28985791713099	27.796227585857565	18.2939608448992
20	19.376677894843482	33.63704285280114	28.453772414142435	18.532506838212946
21	19.22535077499747	35.81434885016716	26.42130356600142	18.538996808833957
22	18.201518336541383	33.074631496302295	26.96266209097356	21.76118807618276
23	17.513739742680578	33.905347735791715	26.288813190152972	22.292099331374736
24	14.944186252659305	36.337503799007195	27.215137777327524	21.50317217100598
25	16.0422576233411	36.77533937797589	25.501943825346974	21.680459173336033
26	19.28566001418296	34.87694382534697	24.16295208185594	21.674444078614123
27	15.950448282848747	35.412603839529936	25.957824688481413	22.679123189139904
28	17.25936455273022	33.96106650795259	28.370035963934757	20.409532975382433
29	18.326885573903354	33.70684960996859	25.61892158849154	22.34734322763651
30	17.893323878026543	33.75971912673488	26.7760358626279	21.57092113261068
31	19.32649934150542	34.69934023908419	22.77932200384966	23.194838415560735
32	19.331564684429136	32.91507319420525	24.76446155404721	22.988900567318407
33	16.335256053084795	33.463554857663866	27.337655759294904	22.863533329956436
34	16.827069192584336	32.19278695167663	26.22612957147199	24.754014284267043
35	18.54912749468139	32.35202866984095	26.788066052071724	22.310777783405936
36	17.356714112045385	34.80856169587681	27.716923310708136	20.117800881369668
37	16.967157582818356	34.1315469557289	26.63151529733563	22.269780164117112
38	16.804275149427617	33.448358828892715	28.4941368655658	21.253229156113868
39	16.462681086009525	33.525288724546655	27.241889119643396	22.770141069800427
40	19.633427464289333	34.46665104852599	26.1555313544727	19.744390132711985
41	16.354409381015095	33.28341859993922	28.65242883193192	21.709743187113766
42	18.76836186809847	33.25635067369061	24.34419638334515	23.63109107486577
43	18.219247036774387	33.154568939317194	26.379514486880762	22.246669537027657
44	19.033975787660825	30.840498683010843	25.370403201296725	24.75512232803161
45	19.793777226218214	32.46109183466721	26.354504356194912	21.390626582919666
46	16.37878634383548	33.557421993718975	27.569553490021274	22.49423817242427
47	17.5954183973255	32.60355460439672	27.746682200384964	22.054344797892817
48	17.892057542295614	31.515297335629622	29.793872201398035	20.79877292067673
49	18.57160495390538	32.40094088744808	26.25921259244251	22.768241566204033
50	17.50186784520312	30.403929439773076	27.38894235639753	24.70526035862628
51	17.47448333502178	29.78738223077702	28.92611564177895	23.812018792422247
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19629.0
1	14245.0
2	8861.0
3	5447.0
4	2033.0
5	1743.5
6	1454.0
7	1531.5
8	1609.0
9	1868.5
10	2128.0
11	2745.5
12	3363.0
13	3659.0
14	3955.0
15	4013.5
16	4072.0
17	3913.0
18	3754.0
19	4240.0
20	4726.0
21	4446.5
22	4167.0
23	4626.0
24	5085.0
25	5463.5
26	6605.0
27	7368.0
28	8873.0
29	10378.0
30	13179.0
31	15980.0
32	18637.5
33	21295.0
34	23636.5
35	25978.0
36	27547.0
37	29116.0
38	29710.0
39	30304.0
40	31808.5
41	33313.0
42	35422.5
43	37532.0
44	41325.5
45	45119.0
46	48208.5
47	51298.0
48	55613.0
49	59928.0
50	56715.0
51	53502.0
52	46618.5
53	39735.0
54	34006.0
55	28277.0
56	24712.0
57	21147.0
58	18457.0
59	15767.0
60	13342.5
61	10918.0
62	9946.0
63	8974.0
64	7383.0
65	5792.0
66	4596.5
67	3401.0
68	2760.5
69	2120.0
70	1769.0
71	1418.0
72	1215.0
73	1012.0
74	814.5
75	510.5
76	404.0
77	266.0
78	128.0
79	90.5
80	53.0
81	74.5
82	96.0
83	78.0
84	60.0
85	38.5
86	17.0
87	10.5
88	4.0
89	5.0
90	6.0
91	6.5
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	631744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.971899124321567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83794448679667	21.971692774673723
2	9.856757673623461	5.711379780262028
3	3.8306656653934885	3.3294497771034695
4	1.998239204249844	2.3157113860716434
5	1.3186349527553158	1.9101679416515773
6	0.9257691175246274	1.609277369120142
7	0.714014267028164	1.448044452236646
8	0.5504513396659176	1.2758096548518907
9	0.44188517406994704	1.1522027418919005
>10	3.5034950356862002	21.617480252950795
>50	0.6230948566110623	12.713266736729079
>100	0.39013094778867907	18.59526179211189
>500	0.0044586394032991895	0.7219281841609916
>1k	0.003901309477886791	2.830875626193358
>5k	0.0	0.0
>10k+	5.573299254123987E-4	2.7974515299908695
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17325	2.742408317293081	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3939	0.6235120555161584	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	3890	0.6157557491642185	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2612	0.41345861614831325	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGT	2023	0.3202246479586668	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1764	0.27922702866984095	No Hit
AATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	1745	0.27621948130888463	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCC	1559	0.24677717556478573	No Hit
AAACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	650	0.10288977813797993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7357410596697397	0.0
2	0.0	0.0	0.0	0.9654227028669841	0.0
3	0.0	0.0	0.0	1.2769412926755142	0.0
4	0.0	0.0	0.0	2.6881141728295006	0.0
5	0.0	0.0	0.0	2.885662546854422	0.0
6	0.0	0.0	0.0	3.9842087934353154	0.0
7	0.0	0.0	0.0	4.872701600648364	0.0
8	0.0	0.0	0.0	5.7032595481714115	0.0
9	0.0	0.0	0.0	7.157962719076082	0.0
10	1.58291966366123E-4	0.0	0.0	9.08959958464188	0.0
11	1.58291966366123E-4	0.0	0.0	10.800102573194206	0.0
12	1.58291966366123E-4	0.0	0.0	11.608974521325093	0.0
13	1.58291966366123E-4	0.0	0.0	11.953259548171411	0.0
14	1.58291966366123E-4	0.0	0.0	12.281082210515653	0.0
15	1.58291966366123E-4	0.0	0.0	12.582944990375848	0.0
16	1.58291966366123E-4	0.0	0.0	13.180338871441597	0.0
17	1.58291966366123E-4	0.0	0.0	14.02007775301388	0.0
18	1.58291966366123E-4	0.0	0.0	15.194287559517779	0.0
19	1.58291966366123E-4	0.0	0.0	15.622783912470874	0.0
20	1.58291966366123E-4	0.0	0.0	16.167466568736703	0.0
21	1.58291966366123E-4	0.0	0.0	16.64440026339783	0.0
22	1.58291966366123E-4	0.0	0.0	17.21156037888765	0.0
23	1.58291966366123E-4	0.0	0.0	17.75671791105258	0.0
24	1.58291966366123E-4	0.0	0.0	18.125538192685646	0.0
25	1.58291966366123E-4	0.0	0.0	18.4397477459224	0.0
26	1.58291966366123E-4	0.0	0.0	18.747783912470876	0.0
27	3.16583932732246E-4	0.0	0.0	19.133066558606018	0.0
28	3.16583932732246E-4	0.0	0.0	19.482733512308783	0.0
29	3.16583932732246E-4	0.0	0.0	19.857885472596493	0.0
30	3.16583932732246E-4	0.0	0.0	20.37834945800831	0.0
31	3.16583932732246E-4	0.0	0.0	20.738938557390334	0.0
32	3.16583932732246E-4	0.0	0.0	21.073409482321953	0.0
33	3.16583932732246E-4	0.0	0.0	21.416586465403707	0.0
34	3.16583932732246E-4	0.0	0.0	21.78144944787762	0.0
35	3.16583932732246E-4	0.0	0.0	22.15707628406443	0.0
36	3.16583932732246E-4	0.0	0.0	22.527796069293892	0.0
37	3.16583932732246E-4	0.0	0.0	22.8557770236045	0.0
38	3.16583932732246E-4	0.0	0.0	23.220006838212946	0.0
39	3.16583932732246E-4	0.0	0.0	23.567932580285685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	35	1.2107739E-7	45.000004	13
ATATCCG	55	1.8189894E-12	45.000004	1
GTCCGCA	35	1.2107739E-7	45.000004	36
CTATTCG	35	1.2107739E-7	45.000004	1
CCGGTGT	35	1.2107739E-7	45.000004	41
GTCGGAT	35	1.2107739E-7	45.000004	17
TATCCGG	70	0.0	45.000004	2
ACGCGGA	55	1.8189894E-12	45.000004	3
GCCCCGA	20	7.030872E-4	45.0	35
CGGGTTA	40	6.8066583E-9	45.0	6
AGCGTTG	20	7.030872E-4	45.0	1
AACGTCA	20	7.030872E-4	45.0	40
ACTATCG	25	3.8888043E-5	45.0	2
AATCCGT	20	7.030872E-4	45.0	28
AATCCCG	50	2.1827873E-11	45.0	1
TCGTTCA	20	7.030872E-4	45.0	16
ACCGGTC	20	7.030872E-4	45.0	16
TACCCGA	25	3.8888043E-5	45.0	34
ACGTTGA	20	7.030872E-4	45.0	17
ACTTGCG	20	7.030872E-4	45.0	1
>>END_MODULE