Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553357_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 520097 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 10200 | 1.9611726274137324 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2412 | 0.4637596448354826 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2146 | 0.41261533906175196 | No Hit |
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT | 1347 | 0.25899014991434294 | Illumina Single End Adapter 1 (95% over 22bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1251 | 0.2405320545975078 | No Hit |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 1241 | 0.23860933633533746 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 1208 | 0.2322643660701754 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 619 | 0.11901626042834317 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTGATAC | 20 | 7.029721E-4 | 45.0 | 25 |
AGCGTGC | 25 | 3.8878505E-5 | 45.0 | 12 |
CTATGCA | 20 | 7.029721E-4 | 45.0 | 11 |
AGCGTAA | 45 | 3.8380676E-10 | 45.0 | 37 |
ATTAGCG | 50 | 2.1827873E-11 | 45.0 | 1 |
TCCGATA | 40 | 6.8030204E-9 | 45.0 | 30 |
CCGATAT | 40 | 6.8030204E-9 | 45.0 | 31 |
GACGTGA | 20 | 7.029721E-4 | 45.0 | 10 |
TATGGCA | 25 | 3.8878505E-5 | 45.0 | 18 |
CGTTGAC | 20 | 7.029721E-4 | 45.0 | 15 |
ATTACCG | 50 | 2.1827873E-11 | 45.0 | 1 |
ACTCTAT | 25 | 3.8878505E-5 | 45.0 | 38 |
TTGTCGG | 20 | 7.029721E-4 | 45.0 | 10 |
TCTTACC | 35 | 1.21021E-7 | 45.0 | 4 |
ATAGCGC | 25 | 3.8878505E-5 | 45.0 | 20 |
CCAATCG | 20 | 7.029721E-4 | 45.0 | 11 |
ATTCGGA | 25 | 3.8878505E-5 | 45.0 | 40 |
GTAGGTC | 20 | 7.029721E-4 | 45.0 | 21 |
TCGATTG | 25 | 3.8878505E-5 | 45.0 | 1 |
GCTTGTA | 25 | 3.8878505E-5 | 45.0 | 25 |