FastQCFastQC Report
Sat 18 Jun 2016
SRR3553357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520097
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102001.9611726274137324No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24120.4637596448354826No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21460.41261533906175196No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT13470.25899014991434294Illumina Single End Adapter 1 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12510.2405320545975078No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12410.23860933633533746No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12080.2322643660701754No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6190.11901626042834317No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC207.029721E-445.025
AGCGTGC253.8878505E-545.012
CTATGCA207.029721E-445.011
AGCGTAA453.8380676E-1045.037
ATTAGCG502.1827873E-1145.01
TCCGATA406.8030204E-945.030
CCGATAT406.8030204E-945.031
GACGTGA207.029721E-445.010
TATGGCA253.8878505E-545.018
CGTTGAC207.029721E-445.015
ATTACCG502.1827873E-1145.01
ACTCTAT253.8878505E-545.038
TTGTCGG207.029721E-445.010
TCTTACC351.21021E-745.04
ATAGCGC253.8878505E-545.020
CCAATCG207.029721E-445.011
ATTCGGA253.8878505E-545.040
GTAGGTC207.029721E-445.021
TCGATTG253.8878505E-545.01
GCTTGTA253.8878505E-545.025