##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520097
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69823129147063	31.0	31.0	34.0	30.0	34.0
2	31.905248444040247	33.0	31.0	34.0	30.0	34.0
3	32.07247878761077	33.0	31.0	34.0	30.0	34.0
4	35.80024687702486	37.0	35.0	37.0	35.0	37.0
5	35.558370842362095	37.0	35.0	37.0	33.0	37.0
6	32.76367485296012	37.0	35.0	37.0	26.0	37.0
7	34.254291026481596	37.0	35.0	37.0	28.0	37.0
8	35.334366473946204	37.0	35.0	37.0	32.0	37.0
9	37.16399248601703	39.0	37.0	39.0	34.0	39.0
10	36.77397870012709	39.0	37.0	39.0	32.0	39.0
11	36.83637859860757	39.0	37.0	39.0	32.0	39.0
12	36.757847093907486	39.0	35.0	39.0	32.0	39.0
13	36.74380163700233	39.0	37.0	39.0	33.0	39.0
14	37.90132033063063	40.0	37.0	41.0	33.0	41.0
15	37.991668861770016	40.0	37.0	41.0	33.0	41.0
16	37.89577328844427	40.0	37.0	41.0	33.0	41.0
17	37.886965316085266	40.0	37.0	41.0	33.0	41.0
18	37.76030817328306	39.0	37.0	41.0	33.0	41.0
19	37.72691824794221	39.0	36.0	41.0	33.0	41.0
20	37.580755128370285	39.0	36.0	41.0	33.0	41.0
21	37.47840691255669	39.0	36.0	41.0	32.0	41.0
22	37.40781046612459	39.0	36.0	41.0	32.0	41.0
23	37.261126289903615	39.0	35.0	41.0	32.0	41.0
24	37.15784747845114	39.0	35.0	41.0	32.0	41.0
25	37.13498443559567	39.0	35.0	41.0	32.0	41.0
26	37.07808543406326	39.0	35.0	40.0	32.0	41.0
27	36.974210579949506	39.0	35.0	40.0	31.0	41.0
28	36.871448979709555	39.0	35.0	40.0	31.0	41.0
29	36.671488203162106	39.0	35.0	40.0	31.0	41.0
30	36.72301512987001	39.0	35.0	40.0	31.0	41.0
31	36.34810814136594	39.0	35.0	40.0	30.0	41.0
32	36.4542287304099	39.0	35.0	40.0	30.0	41.0
33	36.45969117299273	39.0	35.0	40.0	30.0	41.0
34	36.3884909930263	39.0	35.0	40.0	30.0	41.0
35	36.31597375105029	39.0	35.0	40.0	30.0	41.0
36	36.22354099331471	38.0	35.0	40.0	30.0	41.0
37	36.163694464686394	38.0	35.0	40.0	30.0	41.0
38	36.038958117428095	38.0	35.0	40.0	30.0	41.0
39	35.9703939841991	38.0	35.0	40.0	29.0	41.0
40	35.865613529783865	38.0	35.0	40.0	29.0	41.0
41	35.77543804328808	38.0	35.0	40.0	29.0	41.0
42	35.67143052161424	38.0	35.0	40.0	29.0	41.0
43	35.60414884146611	38.0	34.0	40.0	28.0	41.0
44	35.4087602889461	38.0	34.0	40.0	28.0	41.0
45	35.27050530958648	38.0	34.0	40.0	27.0	41.0
46	35.01608161554479	37.0	34.0	40.0	26.0	41.0
47	34.94068606433031	37.0	34.0	40.0	26.0	41.0
48	34.91274512254445	37.0	34.0	40.0	26.0	41.0
49	34.72806803346299	37.0	33.0	40.0	26.0	41.0
50	34.57611753192193	37.0	33.0	40.0	26.0	41.0
51	33.46012378460172	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	6.0
12	12.0
13	22.0
14	26.0
15	48.0
16	92.0
17	160.0
18	308.0
19	549.0
20	936.0
21	1434.0
22	2010.0
23	2537.0
24	3115.0
25	3691.0
26	4334.0
27	5274.0
28	6535.0
29	8357.0
30	10809.0
31	14058.0
32	18623.0
33	25567.0
34	37551.0
35	49821.0
36	54473.0
37	74194.0
38	98571.0
39	96962.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.245838757001096	33.668334945212145	21.207582431738693	24.878243866048063
2	23.37083274850653	32.34704295544869	19.800729479308668	24.48139481673611
3	20.868607202118067	33.73582235621432	19.530010747995085	25.865559693672523
4	21.94840577815292	33.28802127295486	19.022028583129686	25.741544365762543
5	17.844171375724144	35.80601310909311	16.556334683722458	29.793480831460283
6	17.216980678604184	46.37846401728908	17.002213048719756	19.402342255386976
7	82.32156693847494	9.555140675681653	4.381105832181305	3.7421865536621053
8	83.52403493963627	8.867576625129542	3.7070008094643883	3.901387625769808
9	76.436510881624	12.52285631334155	6.166926554085103	4.8737062509493425
10	36.08596088806511	37.825251828024385	11.904317848401357	14.184469435509145
11	27.162817705158844	31.560651186221033	21.022424663091694	20.25410644552843
12	26.178001411275204	27.62004010790295	27.30759839030027	18.894360090521577
13	25.314124096082075	28.592358733082484	23.933612383843784	22.159904786991657
14	20.12778385570384	31.994800969819092	25.91132038831218	21.966094786164888
15	21.458304893125703	31.031519120471756	26.422763446049487	21.08741254035305
16	21.514448266381077	32.2070690659627	24.862669848124487	21.41581281953174
17	21.259495824817293	31.165340311518815	25.854792471404377	21.720371392259523
18	23.36814094293949	31.27070527228575	24.07454763246087	21.286606152313894
19	23.76210591485819	32.3257007827386	23.655395051307735	20.256798251095468
20	25.190493311824525	30.18783034703142	23.001286298517392	21.620390042626664
21	24.81633233800618	31.031134576819326	24.130691005716244	20.021842079458256
22	23.20355625969771	29.45104470896775	23.623670199981927	23.72172883135261
23	22.70864857901507	29.426626187038185	24.700584698623526	23.16414053532322
24	21.156245854138746	30.708117908774707	25.5875346329627	22.548101604123847
25	22.346023914769745	32.30378179454986	23.89496574677416	21.455228543906234
26	23.636744684164686	30.743303652972426	23.18009909689923	22.439852565963655
27	22.429085343695505	30.312422490420055	24.326231452978963	22.932260712905478
28	23.31372801612007	31.43182906265562	25.716356756528107	19.5380861646962
29	22.544063895773288	32.17111423446011	22.804976763949803	22.4798451058168
30	23.653856876698	30.699081132942506	24.589259311243865	21.057802679115625
31	23.37064047668031	31.191104736231896	22.042042157520616	23.396212629567177
32	22.646544779146968	30.827326441029268	23.76287500216306	22.763253777660704
33	23.6448201008658	31.118618257748075	23.22759023797484	22.008971403411287
34	22.029544488816512	31.38203065966541	24.96322801323599	21.62519683828209
35	23.200095366825803	30.971530310692042	24.1178087933597	21.71056552912245
36	25.57772876982563	31.631791761921335	22.659811535155942	20.130667933097097
37	23.86304862362213	32.23091077241361	22.312376345181764	21.593664258782496
38	23.019359850181793	31.108812394611007	24.201062494111675	21.670765261095525
39	22.445620720750167	30.322997440861993	24.1528022657312	23.078579572656636
40	24.09954296986908	29.226471215946255	25.10608598011525	21.567899834069415
41	20.486370811598604	31.312812802227274	25.521777668396474	22.679038717777644
42	22.55752292360848	29.919226605806227	24.95534486836109	22.5679056022242
43	21.992243754530406	30.094770783142376	25.08647425384111	22.826511208486107
44	23.762298186684408	27.798852906284786	25.31585454251803	23.12299436451277
45	23.150296963835594	29.38067322057232	25.24259897672934	22.22643083886275
46	21.523677314039496	29.54122019546354	25.847293870181907	23.087808620315055
47	22.55386975891036	30.330303770258237	25.02610090040896	22.08972557042244
48	22.991480435380325	29.0666933283599	25.568307450340992	22.37351878591878
49	21.867267067489333	29.645046981620737	25.693860952860714	22.793824998029212
50	21.757095311066973	28.153594425655214	25.77538420717674	24.313926056101074
51	23.04608563402596	27.668300336283423	26.20222766137855	23.083386368312063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12051.0
1	9257.0
2	6463.0
3	4062.0
4	1661.0
5	1366.0
6	1071.0
7	1111.5
8	1152.0
9	1167.0
10	1182.0
11	1307.0
12	1432.0
13	1479.0
14	1526.0
15	1640.0
16	1754.0
17	1842.0
18	1930.0
19	1702.0
20	1474.0
21	1645.5
22	1817.0
23	2863.5
24	3910.0
25	4410.5
26	4744.5
27	4578.0
28	6696.0
29	8814.0
30	9532.0
31	10250.0
32	12047.5
33	13845.0
34	14298.0
35	14751.0
36	14378.5
37	14006.0
38	15671.5
39	17337.0
40	18271.0
41	19205.0
42	20691.0
43	22177.0
44	26608.0
45	31039.0
46	33144.0
47	35249.0
48	37102.0
49	38955.0
50	38004.5
51	37054.0
52	35162.0
53	33270.0
54	30298.5
55	27327.0
56	25479.5
57	23632.0
58	23280.0
59	22928.0
60	23470.5
61	24013.0
62	21720.0
63	19427.0
64	17113.0
65	14799.0
66	13646.5
67	12494.0
68	11231.0
69	9968.0
70	8742.0
71	7516.0
72	6599.5
73	5683.0
74	5069.5
75	3234.0
76	2012.0
77	1691.5
78	1371.0
79	1095.0
80	819.0
81	562.0
82	305.0
83	314.0
84	323.0
85	203.0
86	83.0
87	67.0
88	51.0
89	30.0
90	9.0
91	10.5
92	12.0
93	7.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	520097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.31685517986062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41924909021458	18.825926682603043
2	8.700791694351041	4.231517831633369
3	3.23406284409914	2.3592671346758087
4	1.7203211355282582	1.6733119966197614
5	1.102349538026972	1.340283703689407
6	0.7922320839550379	1.1558755712624305
7	0.5299073041490332	0.9019975421619678
8	0.4613542188707546	0.8974946981518288
9	0.386448825328583	0.8457498107948165
>10	3.7871820064025394	21.901432669619254
>50	1.304358565800286	22.509438745173213
>100	0.5553683081018933	19.400425935895324
>500	7.967981464876518E-4	0.1199351195901167
>1k	0.0047807888789259114	1.8610287942860597
>5k	0.0	0.0
>10k+	7.967981464876518E-4	1.9763137638436032
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10200	1.9611726274137324	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2412	0.4637596448354826	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2146	0.41261533906175196	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	1347	0.25899014991434294	Illumina Single End Adapter 1 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1251	0.2405320545975078	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1241	0.23860933633533746	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1208	0.2322643660701754	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	619	0.11901626042834317	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30571220368508184	0.0
2	0.0	0.0	0.0	0.40088675766251297	0.0
3	0.0	0.0	0.0	0.5097126113013534	0.0
4	0.0	0.0	0.0	1.0280774547824734	0.0
5	0.0	0.0	0.0	1.1217138341501682	0.0
6	0.0	0.0	0.0	1.657383141990821	0.0
7	0.0	0.0	0.0	2.089994750979144	0.0
8	0.0	0.0	0.0	2.5354885723240086	0.0
9	0.0	0.0	0.0	3.096922304877744	0.0
10	0.0	0.0	0.0	4.045206951780148	0.0
11	0.0	0.0	0.0	4.762765407222115	0.0
12	0.0	0.0	0.0	5.172688940716828	0.0
13	0.0	0.0	0.0	5.349579020836498	0.0
14	0.0	0.0	0.0	5.5356981486145855	0.0
15	0.0	0.0	0.0	5.677979300015189	0.0
16	0.0	0.0	0.0	5.990421017617868	0.0
17	0.0	0.0	0.0	6.359198380302136	0.0
18	0.0	0.0	0.0	6.931014791471591	0.0
19	0.0	0.0	0.0	7.124055704993491	0.0
20	0.0	0.0	0.0	7.404388027617925	0.0
21	0.0	0.0	0.0	7.652034139785463	0.0
22	0.0	0.0	0.0	7.942556869199399	0.0
23	0.0	0.0	0.0	8.234040957744421	0.0
24	0.0	0.0	0.0	8.433426841531483	0.0
25	0.0	0.0	0.0	8.612239639913323	0.0
26	0.0	0.0	0.0	8.759327586969354	0.0
27	0.0	0.0	0.0	8.98120927442381	0.0
28	0.0	0.0	0.0	9.159637529153215	0.0
29	0.0	0.0	0.0	9.337873512056404	0.0
30	0.0	0.0	0.0	9.579751469437431	0.0
31	0.0	0.0	0.0	9.764140150779566	0.0
32	0.0	0.0	0.0	9.956604248822815	0.0
33	0.0	0.0	0.0	10.153682870695274	0.0
34	0.0	0.0	0.0	10.34114790125688	0.0
35	0.0	0.0	0.0	10.5174611658979	0.0
36	0.0	0.0	0.0	10.68012313087751	0.0
37	0.0	0.0	0.0	10.863742724914776	0.0
38	0.0	0.0	0.0	11.051400027302599	0.0
39	0.0	0.0	0.0	11.258861327790777	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATAC	20	7.029721E-4	45.0	25
AGCGTGC	25	3.8878505E-5	45.0	12
CTATGCA	20	7.029721E-4	45.0	11
AGCGTAA	45	3.8380676E-10	45.0	37
ATTAGCG	50	2.1827873E-11	45.0	1
TCCGATA	40	6.8030204E-9	45.0	30
CCGATAT	40	6.8030204E-9	45.0	31
GACGTGA	20	7.029721E-4	45.0	10
TATGGCA	25	3.8878505E-5	45.0	18
CGTTGAC	20	7.029721E-4	45.0	15
ATTACCG	50	2.1827873E-11	45.0	1
ACTCTAT	25	3.8878505E-5	45.0	38
TTGTCGG	20	7.029721E-4	45.0	10
TCTTACC	35	1.21021E-7	45.0	4
ATAGCGC	25	3.8878505E-5	45.0	20
CCAATCG	20	7.029721E-4	45.0	11
ATTCGGA	25	3.8878505E-5	45.0	40
GTAGGTC	20	7.029721E-4	45.0	21
TCGATTG	25	3.8878505E-5	45.0	1
GCTTGTA	25	3.8878505E-5	45.0	25
>>END_MODULE