FastQCFastQC Report
Sat 18 Jun 2016
SRR3553354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472189
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186783.9556194659341917No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40020.8475419800122409No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT38270.8104805491021603Illumina Paired End PCR Primer 2 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36360.7700306445088725No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT26100.5527447695732005No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC25830.5470267202327881No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT22350.47332741762302805RNA PCR Primer, Index 23 (95% over 21bp)
GAAACAGGGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA20200.4277948025049292No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18860.39941633540806754No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCATTCTAGAATTTGCAGT12140.2571004407133584No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8360.17704774994758454No Hit
GCTCCCGGGACTAATTATTTTTTTTAATTGACTAATTGCCCTGCTGTTTAC6700.14189233548430819No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT6530.13829208219590036No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT5990.12685598351507552RNA PCR Primer, Index 23 (95% over 21bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5970.1264324243046746No Hit
ATATACGGGGACTTTTCAACATTTTAATAAAGATTTTTTAATTATTGGCTA5420.1147845460186493No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT5240.11097251312504103No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5220.11054895391464012No Hit
GCTATAGGGGGTTTGTAATAACTGTGGCACCTCAATATTTTTTATTTGCTT4820.1020777697066217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTG1200.045.0000041
ACTATCA302.1627748E-645.00000437
CAAACCG302.1627748E-645.0000041
TCCGCTC302.1627748E-645.00000416
CTGTACG302.1627748E-645.00000410
CTCCGCT302.1627748E-645.00000415
TAGACTG302.1627748E-645.0000041
TCTTCGG302.1627748E-645.00000412
TAGCGGT302.1627748E-645.0000043
GTCTCCG302.1627748E-645.00000413
GCCATAC302.1627748E-645.00000417
TAGCAAC302.1627748E-645.00000441
AGACGCG302.1627748E-645.0000041
ACCTATC302.1627748E-645.00000434
TCGGACT302.1627748E-645.00000415
AGTTGCG302.1627748E-645.0000041
CCGAGTT302.1627748E-645.00000432
ACTACAC302.1627748E-645.00000424
TACCTAC600.045.00000417
GCGGAAT302.1627748E-645.0000045
Produced by FastQC (version 0.11.5)