##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472189
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34251962667491	31.0	31.0	34.0	30.0	34.0
2	31.536630459413498	31.0	31.0	34.0	30.0	34.0
3	31.715889188439373	31.0	31.0	34.0	30.0	34.0
4	35.48958785571032	37.0	35.0	37.0	33.0	37.0
5	35.202097041650696	37.0	35.0	37.0	32.0	37.0
6	33.819604014494196	37.0	35.0	37.0	30.0	37.0
7	34.671875033090565	37.0	35.0	37.0	32.0	37.0
8	35.38312201258395	37.0	35.0	37.0	32.0	37.0
9	36.98142481082787	39.0	37.0	39.0	33.0	39.0
10	36.376963461664715	38.0	35.0	39.0	32.0	39.0
11	36.47394581407021	38.0	35.0	39.0	32.0	39.0
12	36.50028272577294	38.0	35.0	39.0	32.0	39.0
13	36.4924701761371	38.0	35.0	39.0	32.0	39.0
14	37.56957277700243	39.0	36.0	40.0	32.0	41.0
15	37.6189364851786	39.0	36.0	40.0	32.0	41.0
16	37.45262172562258	39.0	36.0	40.0	32.0	41.0
17	37.40839367287251	39.0	36.0	40.0	32.0	41.0
18	37.17788851498023	39.0	36.0	40.0	32.0	41.0
19	37.04367530798049	39.0	35.0	40.0	32.0	41.0
20	36.998674259671446	39.0	35.0	40.0	31.0	41.0
21	36.96811869823312	39.0	35.0	40.0	31.0	41.0
22	36.92542816541681	39.0	35.0	40.0	31.0	41.0
23	36.758588192439895	39.0	35.0	40.0	31.0	41.0
24	36.5689077890421	38.0	35.0	40.0	31.0	41.0
25	36.60226943024933	38.0	35.0	40.0	31.0	41.0
26	36.544521367503265	38.0	35.0	40.0	31.0	41.0
27	36.45234852993187	38.0	35.0	40.0	31.0	41.0
28	36.328381220231726	38.0	35.0	40.0	30.0	41.0
29	36.0374532231797	38.0	35.0	40.0	30.0	41.0
30	35.958347187249174	38.0	35.0	40.0	30.0	41.0
31	35.88307647996883	38.0	35.0	40.0	30.0	41.0
32	35.79734809578368	38.0	35.0	40.0	29.0	41.0
33	35.67346126233352	38.0	35.0	40.0	29.0	41.0
34	35.692091514203	38.0	35.0	40.0	29.0	41.0
35	35.47370862091239	38.0	34.0	40.0	27.0	41.0
36	35.41783480767235	38.0	34.0	40.0	27.0	41.0
37	35.31706795372192	38.0	34.0	40.0	27.0	41.0
38	35.22686466647889	38.0	34.0	40.0	27.0	41.0
39	35.141140517885844	38.0	34.0	40.0	26.0	41.0
40	35.00341176943978	38.0	34.0	40.0	26.0	41.0
41	34.890514179703466	37.0	33.0	40.0	25.0	41.0
42	34.83212866034575	37.0	33.0	40.0	25.0	41.0
43	34.732359288335815	37.0	33.0	40.0	24.0	41.0
44	34.54970573223857	37.0	33.0	40.0	24.0	41.0
45	34.408264487313346	37.0	33.0	40.0	23.0	41.0
46	34.17087861004809	37.0	33.0	40.0	23.0	41.0
47	34.114814195163376	37.0	33.0	40.0	23.0	41.0
48	34.0209682987109	37.0	33.0	40.0	23.0	41.0
49	33.75511500691461	36.0	32.0	40.0	22.0	41.0
50	33.61441710840363	36.0	32.0	40.0	22.0	41.0
51	32.42383028829558	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	4.0
12	5.0
13	12.0
14	16.0
15	37.0
16	58.0
17	166.0
18	269.0
19	650.0
20	1073.0
21	1726.0
22	2395.0
23	3125.0
24	3801.0
25	4455.0
26	5313.0
27	6287.0
28	7813.0
29	9749.0
30	12633.0
31	15976.0
32	20223.0
33	26233.0
34	35552.0
35	52457.0
36	52206.0
37	62949.0
38	78502.0
39	68492.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.580060103051956	37.21645358108724	20.850337470800888	23.353148845059923
2	20.64914684586045	37.84776858418981	19.291427796920303	22.211656773029446
3	18.81217055035166	37.76390386053043	21.10892036875065	22.31500522036727
4	18.982017793722427	38.30965990313201	17.90151824798968	24.806804055155883
5	15.355292054664552	40.31775411964277	18.377810580085516	25.94914324560716
6	16.97561781405327	44.609467819030094	18.96274584964919	19.452168517267452
7	75.91049346765809	14.486148554921863	4.935100139986319	4.66825783743374
8	77.66614639476988	11.966394812246792	4.445889251973256	5.9215695410100615
9	71.20178572563105	16.927755623277967	7.896414359504352	3.9740442915866314
10	25.736940081196302	49.34041241960317	11.51932806566862	13.403319433531912
11	17.322089248161223	36.02604042025545	29.966178797049487	16.68569153453384
12	21.81774670735659	28.680676593482694	33.65199104595829	15.849585653202425
13	23.75277696007319	31.544148635398113	27.728939047711826	16.97413535681687
14	16.117486853781006	36.20287639059783	29.866854162210473	17.812782593410688
15	14.575519548316457	37.205229262011606	28.87996120197633	19.339289987695604
16	17.20878715937898	37.343944903417906	27.387126764918285	18.060141172284826
17	16.272721304392945	36.04298278867149	26.422258883624988	21.262037023310583
18	18.19102096829871	35.13953099288633	26.77063633417975	19.898811704635218
19	18.188479613036304	39.03161657726037	27.517159442511367	15.26274436719195
20	17.772332688817404	38.465106133349146	25.829699548274103	17.932861629559348
21	17.98220627757106	36.98730804826034	26.659028482239105	18.3714571919295
22	16.289875452414183	37.228948577794064	25.98451043967564	20.49666553011612
23	17.189303435700534	36.09931616365481	24.49781761116841	22.21356278947625
24	14.404825186524887	39.49689636988579	25.065810512316045	21.03246793127328
25	15.97135892619269	39.045382251598404	24.223774802039014	20.75948402016989
26	17.351526613284086	39.10362164302853	22.51916075978051	21.025690983906866
27	14.400589594420879	38.482048501765185	25.352983656967865	21.76437824684607
28	20.761601816221894	35.92036239726042	24.598412923638627	18.71962286287906
29	18.89179968190703	37.98648422559611	22.986134789247526	20.135581303249335
30	20.059552424982368	38.433762751779476	22.22457532894667	19.28210949429148
31	20.562317207728263	38.528640014909286	20.74974215833067	20.159300619031786
32	19.575212467888917	38.65274286355675	21.070588260209366	20.701456408344963
33	18.705857188541028	38.058912850574664	23.910976325157936	19.32425363572637
34	19.59956712248697	35.385195334918855	23.320958345069453	21.694279197524722
35	18.78993369180561	40.04116995525097	21.715457158044764	19.453439194898653
36	19.847561040176707	36.38479507146503	26.430306508622607	17.337337379735658
37	20.238294411771555	41.33366088578938	21.090919102308607	17.337125600130456
38	21.06783512534176	37.73086624211915	24.164264732977685	17.037033899561404
39	20.54410416168102	37.07562014362893	23.948037756068015	18.432237938622034
40	20.92043652012224	37.67855667963464	23.14094568064906	18.260061119594063
41	16.971594001554465	36.0508186340639	27.106730567632876	19.87085679674876
42	20.63474583270682	37.755009117112	23.43044840095809	18.179796649223086
43	18.553587652401898	35.82866182820862	25.37119670301511	20.246553816374377
44	19.57669492512532	34.459718460192846	23.694537568643064	22.26904904603877
45	20.659735826120475	35.46990717699904	24.215938956646596	19.65441804023389
46	18.9222959450559	35.76915175914729	27.395174389915905	17.91337790588091
47	18.814076566798466	36.97290703510671	23.047127315545257	21.165889082549572
48	18.97714686280282	33.319285286188375	28.339076090294352	19.364491760714458
49	20.75990757938029	33.70641840449481	24.416282463166233	21.11739155295867
50	18.833348510871705	33.144567111897985	24.561139713123346	23.460944664106957
51	18.735506333269093	32.658321138357735	27.773412764803922	20.832759763569246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21176.0
1	15938.5
2	10701.0
3	6640.5
4	2580.0
5	2182.5
6	1785.0
7	1828.5
8	1872.0
9	2248.5
10	2625.0
11	3391.5
12	4158.0
13	4585.5
14	5013.0
15	5016.5
16	5020.0
17	4757.5
18	4495.0
19	4287.5
20	4080.0
21	3606.0
22	3132.0
23	3892.5
24	4653.0
25	4576.5
26	5885.5
27	7271.0
28	7737.0
29	8203.0
30	8385.0
31	8567.0
32	9948.5
33	11330.0
34	13040.5
35	14751.0
36	17630.5
37	20510.0
38	18763.0
39	17016.0
40	18523.0
41	20030.0
42	21638.5
43	23247.0
44	30530.5
45	37814.0
46	40131.0
47	42448.0
48	43768.0
49	45088.0
50	42023.5
51	38959.0
52	33043.5
53	27128.0
54	23339.0
55	19550.0
56	17378.5
57	15207.0
58	13244.5
59	11282.0
60	10156.5
61	9031.0
62	7906.5
63	6782.0
64	5635.5
65	4489.0
66	3640.0
67	2791.0
68	2181.0
69	1571.0
70	1555.0
71	1539.0
72	1141.0
73	743.0
74	626.5
75	379.5
76	249.0
77	163.0
78	77.0
79	87.5
80	98.0
81	70.5
82	43.0
83	34.5
84	26.0
85	24.0
86	22.0
87	16.5
88	11.0
89	11.0
90	11.0
91	8.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	472189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.316922397399264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53823461653612	23.204037193034637
2	9.670981637583376	5.86388799626577
3	3.7330691503996176	3.3952550321037127
4	2.0689225382940912	2.5089345615876915
5	1.3090089358085584	1.984256116220513
6	0.9338706760024497	1.6987250888143839
7	0.7295989996422826	1.5483437377362652
8	0.548295447157016	1.3298104417845267
9	0.44403355042043147	1.2115557620944106
>10	3.1508008530132745	19.46537921037098
>50	0.5488180533532369	11.776200180408498
>100	0.3117005097890819	15.852626392189942
>500	0.00562890311131525	1.0544099824613125
>1k	0.006332516000229656	5.122303643302345
>5k	0.0	0.0
>10k+	7.036128889144062E-4	3.9842746616250038
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18678	3.9556194659341917	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4002	0.8475419800122409	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	3827	0.8104805491021603	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3636	0.7700306445088725	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT	2610	0.5527447695732005	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	2583	0.5470267202327881	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	2235	0.47332741762302805	RNA PCR Primer, Index 23 (95% over 21bp)
GAAACAGGGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	2020	0.4277948025049292	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1886	0.39941633540806754	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCATTCTAGAATTTGCAGT	1214	0.2571004407133584	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	836	0.17704774994758454	No Hit
GCTCCCGGGACTAATTATTTTTTTTAATTGACTAATTGCCCTGCTGTTTAC	670	0.14189233548430819	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	653	0.13829208219590036	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	599	0.12685598351507552	RNA PCR Primer, Index 23 (95% over 21bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	597	0.1264324243046746	No Hit
ATATACGGGGACTTTTCAACATTTTAATAAAGATTTTTTAATTATTGGCTA	542	0.1147845460186493	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	524	0.11097251312504103	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	522	0.11054895391464012	No Hit
GCTATAGGGGGTTTGTAATAACTGTGGCACCTCAATATTTTTTATTTGCTT	482	0.1020777697066217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9930345687849569	0.0
2	0.0	0.0	0.0	1.2590297529167347	0.0
3	0.0	0.0	0.0	1.5889823778190513	0.0
4	0.0	0.0	0.0	3.23662770627863	0.0
5	0.0	0.0	0.0	3.5297306798760664	0.0
6	0.0	0.0	0.0	4.802949666341232	0.0
7	0.0	0.0	0.0	5.806784994991412	0.0
8	0.0	0.0	0.0	6.747933560502257	0.0
9	0.0	0.0	0.0	8.579191806670634	0.0
10	0.0	0.0	0.0	10.711812431039267	0.0
11	0.0	0.0	0.0	13.01999834811908	0.0
12	0.0	0.0	0.0	13.946745900476293	0.0
13	0.0	0.0	0.0	14.43828636414656	0.0
14	0.0	0.0	0.0	14.890859380459943	0.0
15	0.0	0.0	0.0	15.22589471588707	0.0
16	0.0	0.0	0.0	15.913331314367763	0.0
17	0.0	0.0	0.0	16.81551243252172	0.0
18	0.0	0.0	0.0	18.158830468308242	0.0
19	0.0	0.0	0.0	18.616062635936036	0.0
20	0.0	0.0	0.0	19.23488264233178	0.0
21	0.0	0.0	0.0	19.756707589545712	0.0
22	0.0	0.0	0.0	20.372139122258247	0.0
23	0.0	0.0	0.0	20.92975482275106	0.0
24	0.0	0.0	0.0	21.307146079218278	0.0
25	0.0	0.0	0.0	21.626721503465774	0.0
26	0.0	0.0	0.0	21.92977811850763	0.0
27	0.0	0.0	0.0	22.31098140786846	0.0
28	0.0	0.0	0.0	22.657664621581613	0.0
29	0.0	0.0	0.0	23.059622312252085	0.0
30	0.0	0.0	0.0	23.54438582855594	0.0
31	0.0	0.0	0.0	23.874550233063456	0.0
32	0.0	0.0	0.0	24.20810311125418	0.0
33	0.0	0.0	0.0	24.544832683522912	0.0
34	0.0	0.0	0.0	24.918623686701725	0.0
35	0.0	0.0	0.0	25.29453248593254	0.0
36	2.1177960520046E-4	0.0	0.0	25.600554015447205	0.0
37	2.1177960520046E-4	0.0	0.0	25.918223423247895	0.0
38	2.1177960520046E-4	0.0	0.0	26.261094604067438	0.0
39	2.1177960520046E-4	0.0	0.0	26.66241695592231	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	120	0.0	45.000004	1
ACTATCA	30	2.1627748E-6	45.000004	37
CAAACCG	30	2.1627748E-6	45.000004	1
TCCGCTC	30	2.1627748E-6	45.000004	16
CTGTACG	30	2.1627748E-6	45.000004	10
CTCCGCT	30	2.1627748E-6	45.000004	15
TAGACTG	30	2.1627748E-6	45.000004	1
TCTTCGG	30	2.1627748E-6	45.000004	12
TAGCGGT	30	2.1627748E-6	45.000004	3
GTCTCCG	30	2.1627748E-6	45.000004	13
GCCATAC	30	2.1627748E-6	45.000004	17
TAGCAAC	30	2.1627748E-6	45.000004	41
AGACGCG	30	2.1627748E-6	45.000004	1
ACCTATC	30	2.1627748E-6	45.000004	34
TCGGACT	30	2.1627748E-6	45.000004	15
AGTTGCG	30	2.1627748E-6	45.000004	1
CCGAGTT	30	2.1627748E-6	45.000004	32
ACTACAC	30	2.1627748E-6	45.000004	24
TACCTAC	60	0.0	45.000004	17
GCGGAAT	30	2.1627748E-6	45.000004	5
>>END_MODULE