FastQCFastQC Report
Sat 18 Jun 2016
SRR3553352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908603
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118811.3076117952505109No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24730.27217607690047246No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22400.2465323138928663No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT17610.1938140199845257Illumina Single End Adapter 1 (95% over 21bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14150.15573358221357403No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12270.1350424772975656No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA9990.10994900963347029No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9120.10037387065638127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCA302.1649976E-645.00000422
ATTACCG600.045.0000041
TGATTCG351.2115561E-745.0000041
TTAACGG700.045.0000042
TCCGTAT650.045.00000423
CGTCGAT302.1649976E-645.00000427
TATCCCG302.1649976E-645.0000041
CCTTACG406.8121153E-945.01
TCGTTAA253.890158E-545.038
ACACGTC207.032506E-445.027
CGTATTT253.890158E-545.09
CAACGAA253.890158E-545.014
CTATCGA207.032506E-445.01
TAGCGGA253.890158E-545.044
TAGCGCA207.032506E-445.023
ATAGCGC253.890158E-545.036
CACGATA253.890158E-545.035
TAACGTA207.032506E-445.034
CACTGCG253.890158E-545.01
TACGGAT207.032506E-445.031